Effectiveness of acidic calcium sulfate with propionic and lactic acid and lactates as postprocessing dipping solutions to control Listeria monocytogenes on frankfurters with or without potassium lactate and stored vacuum packaged at 4.5 degrees C.

The safety of ready-to-eat meat products such as frankfurters can be enhanced by treating with approved antimicrobial substances to control the growth of Listeria monocytogenes. We evaluated the effectiveness of acidic calcium sulfate with propionic and lactic acid, potassium lactate, or lactic acid postprocessing dipping solutions to control L. monocytogenes inoculated (ca. 10(8) CFU/ml) onto the surface of frankfurters with or without potassium lactate and stored in vacuum packages at 4.5 degrees C for up to 12 weeks. Two frankfurter formulations were manufactured without (control) or with potassium lactate (KL, 3.3% of a 60% [wt/wt] commercially available syrup). After cooking, chilling, and peeling, each batch was divided into inoculated (four strains of L. monocytogenes mixture) and noninoculated groups. Each group was treated with four different dips: (i) control (saline solution), (ii) acidic calcium sulfate with propionic and lactic acid (ACS, 1:2 water), (iii) KL, or (iv) lactic acid (LA, 3.4% of a 88% [wt/wt] commercially available syrup) for 30 s. Noninoculated frankfurters were periodically analyzed for pH, water activity, residual nitrite, and aerobic plate counts (APCs), and L. monocytogenes counts (modified Oxford medium) were determined on inoculated samples. Surface APC counts remained at or near the lower limit of detection (<2 log CFU per frank) on franks with or without KL and treated with ACS or LA throughout 12 weeks at 4.5 degrees C. L. monoctogenes counts remained at the minimum level of detection on all franks treated with the ACS dip, which indicated a residual bactericidal effect when L. monocytogenes populations were monitored over 12 weeks. L. monocytogenes numbers were also reduced, but not to the same degree in franks made without or with KL and treated with LA. These results revealed the effectiveness of ACS (bactericidal effect) or LA (bacteriostatic effect) as postprocessing dipping solutions to inhibit or control the growth of L. monocytogenes on vacuum-packaged frankfurters stored at 4.5 degrees C for up to 12 weeks.

Rate of Leaf Appearance in Crimson Clover.

Understanding factors that affect growth and development of crimson clover (Trifolium incarnatum L.) are important for the development of management practices to optimize forage utilization. In a 3-yr field experiment at College Station, TX, we evaluated the effects of planting date on rate of leaf appearance of an intermediate- and late-maturing crimson clover. We wanted to determine if growing degree days (GDD) or a photothermal index (PTI) could be used to predict growth. Leaf appearance rates (LAR) did not differ between 'Tibbee' and 'Columbus' crimson clover. Leaf appearance rate was primarily controlled by temperature or GDD, which accounted for 90 to 99% of the variability within each planting date. Photoperiod did not consistently influence the rate of leaf appearance under normal daylengths of 10 h 12 min to 14 h 6 min used in this study. Predictions of LAR were not improved when photoperiod was combined with temperature in a photothermal index than with predictions that used GDD alone. Leaf appearance rate of crimson clover was generally higher when planted in October, November, and December and lower when planted in September, February, and March.

Flowering in Crimson Clover as Affected by Planting Date.

Understanding factors that affect flowering of crimson clover (Trifolium incarnatum L.) could improve management decisions to optimize utilization by improving season of use. The experiment was a split-plot randomized complete block design with three replications at College Station, TX, in the 1997-1998 and 1999-2000 growing seasons, and Overton, TX, in the 1998-1999 growing season. Main plot treatments of two crimson clover cultivars and subplot treatments of six planting dates (PDs) were used to evaluate the effect of date to reach 50% budding and 50% flowering based on day of year (DOY), days after planting (DAP), photothermal index (PTI), and growing degree days (GDD) under field conditions. Correlations with 50% bud and 50% flower were almost identical. 'Columbus' planted in the autumn flowered an average of 49 d later than 'Tibbee'. Date to reach 50% flowering was best correlated with DOY (r = 0.93 and 0.97) and DAP (r = 0.92 and 0.98) for Columbus and Tibbee. Date to reach flowering was not as highly correlated with PTI (r = 0.66 and 0.82) or GDD (r = 0.71 and 0.85) for Columbus and Tibbee, thus temperature could not be used to predict flowering. Planting after 21 December delayed flowering in Tibbee 2 to 9 wks, whereas, Columbus planted after 21 December did not flower. It is important to plant early in the growing season or to use later-maturing cultivars to maximize the length of the growing season and possible total production in grazed environments.