1.
Plast Reconstr Surg
; 111(5): 1759-60, 2003 Apr 15.
Artigo
em Inglês
| MEDLINE
| ID: mdl-12655230
2.
Plast Reconstr Surg
; 111(2): 955, 2003 Feb.
Artigo
em Inglês
| MEDLINE
| ID: mdl-12560741
3.
Mol Gen Genet
; 240(2): 286-9, 1993 Aug.
Artigo
em Inglês
| MEDLINE
| ID: mdl-8355660
RESUMO
gdhA1 is a spontaneous mutant of Escherichia coli that causes complete loss of activity of the NADP-specific glutamate dehydrogenase (GDH) encoded by the gdhA gene. The gdhA1 mutational site has been identified by recombinational mapping, polymerase chain reaction (PCR) amplification and DNA sequencing, as an A to G transition at nucleotide 274 of the gdhA coding sequence, resulting in an amino acid change of lysine 92 to glutamic acid. The mutant enzyme forms hybrid hexamers with a wild-type GDH, providing a useful system for analysis of conformational integrity of mutational variants.