Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 46
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
Int J Mol Sci ; 24(10)2023 May 14.
Artigo em Inglês | MEDLINE | ID: mdl-37240095

RESUMO

Repeated anodal transcranial direct current stimulation (RA-tDCS) is a neuromodulatory technique consisting of stimulating the cerebral cortex with a weak electric anodal current in a non-invasive manner. RA-tDCS over the dorsolateral prefrontal cortex has antidepressant-like properties and improves memory both in humans and laboratory animals. However, the mechanisms of action of RA-tDCS remain poorly understood. Since adult hippocampal neurogenesis is thought to be involved in the pathophysiology of depression and memory functioning, the purpose of this work was to evaluate the impact of RA-tDCS on hippocampal neurogenesis levels in mice. RA-tDCS was applied for 20 min per day for five consecutive days over the left frontal cortex of young adult (2-month-old, high basal level of neurogenesis) and middle-aged (10-month-old, low basal level of neurogenesis) female mice. Mice received three intraperitoneal injections of bromodeoxyuridine (BrdU) on the final day of RA-tDCS. The brains were collected either 1 day or 3 weeks after the BrdU injections to quantify cell proliferation and cell survival, respectively. RA-tDCS increased hippocampal cell proliferation in young adult female mice, preferentially (but not exclusively) in the dorsal part of the dentate gyrus. However, the number of cells that survived after 3 weeks was the same in both the Sham and the tDCS groups. This was due to a lower survival rate in the tDCS group, which suppressed the beneficial effects of tDCS on cell proliferation. No modulation of cell proliferation or survival was observed in middle-aged animals. Our RA-tDCS protocol may, therefore, influence the behavior of naïve female mice, as we previously described, but its effect on the hippocampus is only transient in young adult animals. Future studies using animal models for depression in male and female mice should provide further insights into RA-tDCS detailed age- and sex-dependent effects on hippocampal neurogenesis.


Assuntos
Estimulação Transcraniana por Corrente Contínua , Humanos , Adulto Jovem , Masculino , Feminino , Camundongos , Animais , Lactente , Estimulação Transcraniana por Corrente Contínua/métodos , Córtex Pré-Frontal , Bromodesoxiuridina , Lobo Frontal , Proliferação de Células , Hipocampo
2.
J Chem Neuroanat ; 125: 102161, 2022 11.
Artigo em Inglês | MEDLINE | ID: mdl-36100131

Assuntos
Encéfalo , Hipotálamo
3.
Biomed Pharmacother ; 151: 113100, 2022 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-35597115

RESUMO

Chronic distress-induced hypothalamic-pituitary-adrenal axis deregulations have been associated with the development of neuropsychiatric disorders such as anxiety and depression. Currently available drugs treating such pathological conditions have limited efficacy and diverse side effects, revealing the need of new safer strategies. Aromatic plant-based compounds are largely used in herbal medicine due to their therapeutic properties on mood, physiology, and general well-being. The purpose of this study was to investigate the effects of 2-phenylethyl alcohol (PEA), one of the pharmacologically active constituents of rose essential oil, on chronic corticosterone (CORT)-induced behavioral and neurobiological changes in female mice. Animals followed a prolonged PEA inhalation exposure (30 min per day) for 15 consecutive days prior to behavioral evaluation with open-field, forced swim and novelty-suppressed feeding tests. CORT treatment induced an anxio-depressive-like phenotype, evidenced by a reduced locomotor activity in the open-field, and an increased latency to feed in the novelty-suppressed feeding paradigms. To elucidate the neural correlates of our behavioral results, immunohistochemistry was further performed to provide a global map of neural activity based on cerebral cFos expression. The altered feeding behavior was accompanied by a significant decrease in the number of cFos-positive cells in the olfactory bulb, and altered functional brain connectivity as shown by cross-correlation-based network analysis. CORT-induced behavioral and neurobiological alterations were reversed by prolonged PEA inhalation, suggesting a therapeutic action that allows regulating the activity of neural circuits involved in sensory, emotional and feeding behaviors. These findings might contribute to better understand the therapeutic potential of PEA on anxio-depressive symptoms.


Assuntos
Sistema Hipotálamo-Hipofisário , Álcool Feniletílico , Animais , Ansiedade/induzido quimicamente , Comportamento Animal , Corticosterona/metabolismo , Depressão/induzido quimicamente , Depressão/tratamento farmacológico , Modelos Animais de Doenças , Feminino , Camundongos , Fenótipo , Álcool Feniletílico/farmacologia , Sistema Hipófise-Suprarrenal
4.
J Chem Neuroanat ; 121: 102089, 2022 04.
Artigo em Inglês | MEDLINE | ID: mdl-35283254

RESUMO

The lateral hypothalamus (LHA) is still a poorly understood brain region. Based on published Dlx and Gad gene expression patterns in the embryonic and adult hypothalamus respectively, three large areas are identified in the LHA. A central tuberal LHA region is already well described as it contains neurons producing the peptides melanin-concentrating hormone or hypocretin. This region is rich in GABAergic neurons and is specified by Dlx gene expression in the rodent embryo. Rostrally and caudally bordering the tuberal LHA, two Dlx-GAD-GABA poor regions are then easily delineated. The three regions show different organizational schema. The tuberal region is reticularly organized, connected with the cerebral cortex and the spinal cord, and its embryonic development occurs along the tractus postopticus. The region anterior to it is associated with the stria medullaris in both embryonic and adult subjects. The posterior LHA region is made of differentiated nuclei and includes the subthalamic nucleus. Therefore, the LHA is divided into three distinct parts: in addition to the well-known tuberal LHA, caudal and anterior LHA regions exist that have specific anatomical and functional characteristics. The hypothalamus is made up of several dozens of nuclei or areas that are more or less well differentiated and whose boundaries and arrangements are drawn differently according to authors and atlases (Allen Institute, 2004; Paxinos and Franklin, 2019; Paxinos and Watson, 2013; Swanson, 2004). The dominant hypothesis for more than 50 years is that these structures are distributed within three antero-posterior areas (anterior, tuberal, posterior) and more or less three longitudinal zones (lateral, medial and periventricular) (Fig. 1). In addition to these regions, several adjacent territories are often associated to the hypothalamus. The preoptic area is functionally related to the hypothalamus, but it is better seen as a telencephalic structure based on developmental data (Croizier et al., 2015; Puelles and Rubenstein, 2015). Lately, the zona incerta and the subthalamic nucleus (STN) have also been associated to the hypothalamus on the basis of their connections and development for the STN (Altman and Bayer, 1986; Barbier and Risold, 2021; Swaab et al., 2021). However, the zona incerta is still included in the 'pre-thalamus' or "ventral thalamus" in the embryo (Puelles and Rubenstein, 2015). Thus, the boundaries of the hypothalamus remain blurred around what we can call a 'core' made of the anterior to posterior regions (Brooks, 1988). In addition, unlike other large brain regions that are characterized early on by a molecular signature, i.e. by the embryonic expression of specific molecular markers, data illustrating the distribution of dozens of transcription factors involved in brain patterning and cell lineage specification confirmed the extremely heterogeneous and mosaic nature of the anterior and posterior regions of the hypothalamus (Alvarez-Bolado, 2019; Puelles et al., 2013; Puelles and Rubenstein, 2015). The rich nuclear organization of the medial and periventricular zones of the hypothalamus is consistent with the mosaic expression of developmental genes. The LHA, however, is often perceived as much more homogeneous in its cytoarchitectural organization. At the same time, there is little information regarding the expression of developmental genes in the anterior and posterior territories of the LHA. Most studies focus on the tuberal LHA which expresses many of these genes. Admittedly, even in the adult hypothalamus, the internal boundaries of the LHA are difficult to identify and the same is true in the embryo. Developmental data alone are insufficient to achieve a better understanding of the LHA anatomical organization and for this region as for medial and periventricular zones, a coherence must be established between development and adult anatomical organization. Among the most useful neurochemical markers to identify large regions of the forebrain, those involved in the identification of GABAergic and glutamatergic neurons have proven to be particularly efficient. Indeed, GABAergic neurons are not ubiquitously distributed. Large regions of the forebrain are rich in such cells, including the basal telencephalon, but others contain few or no GABAergic cells and are rich in glutamatergic neurons instead (for example the dorsal thalamus that is free of GABA-neurons in rodents). The same applies for the hypothalamus: several structures of the hypothalamus are free of GABAergic neurons, as, for example, the mammillary nuclei (Hahn et al., 2019). Recently, we also identified a GABA-poor posterior LHA territory that includes the (STN), and is localized caudal to the GABA-rich tuberal LHA (Barbier et al., 2020; Barbier and Risold, 2021; Chometton et al., 2016b). Therefore, the LHA seems partitioned into GABA-rich/GABA-poor regions. However, to define or confirm distinct neuroanatomical entities, these regions must have a specific embryological origin, and show specific hodological patterns and functions. Hence, the purpose of this short review is to identify divisions of the LHA based on developmental and neurochemical criteria. Such an analysis seems to us relevant in order to allow later functional studies on regions whose boundaries will be based on objective criteria.


Assuntos
Glutamato Descarboxilase , Roedores , Animais , Feminino , Glutamato Descarboxilase/metabolismo , Humanos , Hipotálamo/metabolismo , Gravidez , Prosencéfalo/metabolismo , Fatores de Transcrição/metabolismo , Ácido gama-Aminobutírico
6.
Front Behav Neurosci ; 15: 717701, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34588963

RESUMO

In humans and mammals, effort-based decision-making for monetary or food rewards paradigms contributes to the study of adaptive goal-directed behaviours acquired through reinforcement learning. Chronic distress modelled by repeated exposure to glucocorticoids in rodents induces suboptimal decision-making under uncertainty by impinging on instrumental acquisition and prompting negative valence behaviours. In order to further disentangle the motivational tenets of adaptive decision-making, this study addressed the consequences of enduring distress on relevant effort and reward-processing dimensions. Experimentally, appetitive and consummatory components of motivation were evaluated in adult C57BL/6JRj male mice experiencing chronic distress induced by oral corticosterone (CORT), using multiple complementary discrete behavioural tests. Behavioural data (from novelty suppressed feeding, operant effort-based choice, free feeding, and sucrose preference tasks) collectively show that behavioural initiation, effort allocation, and hedonic appreciation and valuation are altered in mice exposed to several weeks of oral CORT treatment. Additionally, data analysis from FosB immunohistochemical processing of postmortem brain samples highlights CORT-dependent dampening of neural activation in the anterior insular cortex (aIC) and basolateral amygdala (BLA), key telencephalic brain regions involved in appetitive and consummatory motivational processing. Combined, these results suggest that chronic distress-induced irregular aIC and BLA neural activations with reduced effort production and attenuated reward value processing during reinforcement-based instrumental learning could result in maladaptive decision-making under uncertainty. The current study further illustrates how effort and reward processing contribute to adjust the motivational threshold triggering goal-directed behaviours in versatile environments.

7.
eNeuro ; 8(5)2021.
Artigo em Inglês | MEDLINE | ID: mdl-34518367

RESUMO

The subthalamic nucleus (STN) is an essential component of the basal ganglia and has long been considered to be a part of the ventral thalamus. However, recent neurodevelopmental data indicated that this nucleus is of hypothalamic origin which is now commonly acknowledged. In this work, we aimed to verify whether the inclusion of the STN in the hypothalamus could influence the way we understand and conduct research on the organization of the whole ventral and posterior diencephalon. Developmental and neurochemical data indicate that the STN is part of a larger glutamatergic posterior hypothalamic region that includes the premammillary and mammillary nuclei. The main anatomic characteristic common to this region involves the convergent cortical and pallidal projections that it receives, which is based on the model of the hyperdirect and indirect pathways to the STN. This whole posterior hypothalamic region is then integrated into distinct functional networks that interact with the ventral mesencephalon to adjust behavior depending on external and internal contexts.


Assuntos
Núcleo Subtalâmico , Gânglios da Base , Globo Pálido , Hipotálamo , Vias Neurais
8.
Handb Clin Neurol ; 180: 173-184, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34225928

RESUMO

The zona incerta (ZI) is a large structure made of four neurochemically defined regions (at least, in rodents). It is globally involved in complex connections with telencephalic and brainstem centers. In this work, we focus on some of the anatomical links this structure develops with the cerebral cortex and the tectum. We also point to its integration within a larger basal ganglia network. The functions of this region are still mysterious, even if recent works suggest its participation in behavioral expression. Studies about the functional organization of the vibrissal system have provided the first integrated model, illustrating the ZI's role in sensory-motor programing. In addition, ZI connections with the superior colliculus and the cerebral cortex as well as recent behavioral studies point to this region playing a role in cognitive processes related to attention toward salient stimuli.


Assuntos
Zona Incerta , Atenção , Tronco Encefálico , Córtex Cerebral , Humanos , Movimento
9.
J Chem Neuroanat ; 116: 101997, 2021 10.
Artigo em Inglês | MEDLINE | ID: mdl-34182088

RESUMO

In the last few years we assist to an unexpected deluge of genomic data on hypothalamic development and structure. Perhaps most surprisingly, the Lateral Zone has received much attention too. The new information focuses first of all on transcriptional heterogeneity. Many already known and a number of hitherto unknown lateral hypothalamic neurons have been described to an enormous degree of detail. Maybe the most surprising novel discoveries are two: First, some restricted regions of the embryonic forebrain neuroepithelium generate specific LHA neurons, either GABAergic or glutamatergic. Second, evidence is mounting that supports the existence of numerous kinds of "bilingual" lateral hypothalamic neurons, expressing (and releasing) glutamate and GABA both as well as assorted neuropeptides. This is not accepted by all, and it could be that genomic researchers need a common set of rules to interpret their data (sensitivity, significance, age of analysis). In any case, some of the new results appear to confirm hypotheses about the ability of the hypothalamus and in particular its Lateral Zone to achieve physiological flexibility on a fixed connectivity ("biochemical switching"). Furthermore, the results succinctly reviewed here are the basis for future advances, since the transcriptional databases generated can now be mined e.g. for adhesion genes, to figure out the causes of the peculiar histology of the Lateral Zone; or for ion channel genes, to clarify present and future electrophysiological data. And with the specific expression data about small subpopulations of neurons, their connections can now be specifically labeled, revealing novel relations with functional significance.


Assuntos
Neurônios GABAérgicos/química , Neurônios GABAérgicos/metabolismo , Ácido Glutâmico/metabolismo , Região Hipotalâmica Lateral/crescimento & desenvolvimento , Região Hipotalâmica Lateral/metabolismo , Neurogênese/fisiologia , Animais , Ácido Glutâmico/análise , Humanos , Região Hipotalâmica Lateral/química , Fatores de Transcrição/análise , Fatores de Transcrição/biossíntese
10.
Eur Neuropsychopharmacol ; 46: 56-67, 2021 05.
Artigo em Inglês | MEDLINE | ID: mdl-33531260

RESUMO

Anxio-depressive symptoms as well as severe cognitive dysfunction including aberrant decision-making (DM) are documented in neuropsychiatric patients with hypercortisolaemia. Yet, the influence of the hypothalamo-pituitary-adrenal (HPA) axis on DM processes remains poorly understood. As a tractable mean to approach this human condition, adult male C57BL/6JRj mice were chronically treated with corticosterone (CORT) prior to behavioural, physiological and neurobiological evaluation. The behavioural data indicate that chronic CORT delays the acquisition of contingencies required to orient responding towards optimal DM performance in a mouse Gambling Task (mGT). Specifically, CORT-treated animals show a longer exploration and a delayed onset of the optimal DM performance. Remarkably, the proportion of individuals performing suboptimally in the mGT is increased in the CORT condition. This variability seems to be better accounted for by variations in sensitivity to negative rather than to positive outcome. Besides, CORT-treated animals perform worse than control animals in a spatial working memory (WM) paradigm and in a motor learning task. Finally, Western blotting neurobiological analyses show that chronic CORT downregulates glucocorticoid receptor expression in the medial Prefrontal Cortex (mPFC). Besides, corticotropin-releasing factor signalling in the mPFC of CORT individuals negatively correlates with their DM performance. Collectively, this study describes how chronic exposure to glucocorticoids induces suboptimal DM under uncertainty in a mGT, hampers WM and motor learning processes, thus affecting specific emotional, motor, cognitive and neurobiological endophenotypic dimensions relevant for precision medicine in biological psychiatry.


Assuntos
Glucocorticoides , Sistema Hipófise-Suprarrenal , Animais , Corticosterona/metabolismo , Corticosterona/farmacologia , Glucocorticoides/metabolismo , Glucocorticoides/farmacologia , Humanos , Sistema Hipotálamo-Hipofisário/metabolismo , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Sistema Hipófise-Suprarrenal/metabolismo , Estresse Psicológico/psicologia
11.
J Comp Neurol ; 529(5): 929-956, 2021 04 01.
Artigo em Inglês | MEDLINE | ID: mdl-32678476

RESUMO

As stressful environment is a potent modulator of feeding, we seek in the present work to decipher the neuroanatomical basis for an interplay between stress and feeding behaviors. For this, we combined anterograde and retrograde tracing with immunohistochemical approaches to investigate the patterns of projections between the dorsomedial division of the bed nucleus of the stria terminalis (BNST), well connected to the amygdala, and hypothalamic structures such as the paraventricular (PVH) and dorsomedial (DMH), the arcuate (ARH) nuclei and the lateral hypothalamic areas (LHA) known to control feeding and motivated behaviors. We particularly focused our study on afferences to proopiomelanocortin (POMC), agouti-related peptide (AgRP), melanin-concentrating-hormone (MCH) and orexin (ORX) neurons characteristics of the ARH and the LHA, respectively. We found light to intense innervation of all these hypothalamic nuclei. We particularly showed an innervation of POMC, AgRP, MCH and ORX neurons by the dorsomedial and dorsolateral divisions of the BNST. Therefore, these results lay the foundation for a better understanding of the neuroanatomical basis of the stress-related feeding behaviors.


Assuntos
Tonsila do Cerebelo/anatomia & histologia , Hipotálamo/anatomia & histologia , Camundongos/anatomia & histologia , Vias Neurais/anatomia & histologia , Núcleos Septais/anatomia & histologia , Proteína Relacionada com Agouti/análise , Animais , Transporte Axonal , Comportamento Alimentar/fisiologia , Comportamento Alimentar/psicologia , Hormônios Hipotalâmicos/análise , Proteínas Luminescentes/análise , Masculino , Melaninas/análise , Camundongos Endogâmicos C57BL , Proteínas do Tecido Nervoso/análise , Neurônios/química , Neurônios/classificação , Neurônios/ultraestrutura , Orexinas/análise , Fito-Hemaglutininas/análise , Hormônios Hipofisários/análise , Pró-Proteína Convertases/análise , Vírus da Raiva , Especificidade da Espécie , Tirosina 3-Mono-Oxigenase/análise , Proteína Vermelha Fluorescente
12.
Proc Natl Acad Sci U S A ; 117(27): 15967-15976, 2020 07 07.
Artigo em Inglês | MEDLINE | ID: mdl-32571909

RESUMO

The insular cortex (INS) is extensively connected to the central nucleus of the amygdala (CEA), and both regions send convergent projections into the caudal lateral hypothalamus (LHA) encompassing the parasubthalamic nucleus (PSTN). However, the organization of the network between these structures has not been clearly delineated in the literature, although there has been an upsurge in functional studies related to these structures, especially with regard to the cognitive and psychopathological control of feeding. We conducted tract-tracing experiments from the INS and observed a pathway to the PSTN region that runs parallel to the canonical hyperdirect pathway from the isocortex to the subthalamic nucleus (STN) adjacent to the PSTN. In addition, an indirect pathway with a relay in the central amygdala was also observed that is similar in its structure to the classic indirect pathway of the basal ganglia that also targets the STN. C-Fos experiments showed that the PSTN complex reacts to neophobia and sickness induced by lipopolysaccharide or cisplatin. Chemogenetic (designer receptors exclusively activated by designer drugs [DREADD]) inhibition of tachykininergic neurons (Tac1) in the PSTN revealed that this nucleus gates a stop "no-eat" signal to refrain from feeding when the animal is subjected to sickness or exposed to a previously unknown source of food. Therefore, our anatomical findings in rats and mice indicate that the INS-PSTN network is organized in a similar manner as the hyperdirect and indirect basal ganglia circuitry. Functionally, the PSTN is involved in gating feeding behavior, which is conceptually homologous to the motor no-go response of the adjacent STN.


Assuntos
Gânglios da Base/fisiologia , Córtex Cerebral/patologia , Comportamento Alimentar/fisiologia , Hipotálamo/fisiologia , Córtex Olfatório/fisiologia , Animais , Comportamento Animal , Núcleo Central da Amígdala , Masculino , Camundongos , Modelos Animais , Vias Neurais/fisiologia , Neurônios/metabolismo , Ratos , Ratos Sprague-Dawley , Núcleo Subtalâmico
13.
Neuroscience ; 409: 261-275, 2019 06 15.
Artigo em Inglês | MEDLINE | ID: mdl-30930128

RESUMO

Injection of the anterograde tracer Phaseolus vulgaris leucoagglutinin (PHAL) into the rat rostral and caudal supramammillary nucleus (SUM) provided expected patterns of projections into the hippocampus and the septal region. In addition, unexpectedly intense projections were observed into the claustrum defined by parvalbumin expression. Injections of the retrograde tracer fluorogold (FG) into the hippocampus and the region of the claustrum showed that the cells of origin of these projections distributed similarly within the borders of the SUM. The SUM is usually involved in control of hippocampal theta activity, but the observation of intense projections into the claustrum indicates that it may also influence isocortical processes. Therefore, the SUM may coordinate sensory processing in the isocortex with memory formation in the hippocampus.


Assuntos
Claustrum/fisiologia , Hipotálamo Posterior/fisiologia , Neurônios/fisiologia , Animais , Claustrum/efeitos dos fármacos , Hipotálamo Posterior/efeitos dos fármacos , Vias Neurais/efeitos dos fármacos , Vias Neurais/fisiologia , Técnicas de Rastreamento Neuroanatômico , Marcadores do Trato Nervoso/farmacologia , Neurônios/efeitos dos fármacos , Ratos
14.
Front Neurol ; 9: 688, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30210427

RESUMO

Projections from the central nucleus of the amygdala (CEA) into the lateral hypothalamic area (LHA) show a very complex pattern. After injection of an anterograde tracer (Phaseolus vulgaris leucoagglutinin-PHAL) into the medial and intermediate parts of the CEA, we observed that labeled axons converged onto the caudal lateral LHA but provided distinct patterns in rostral tuberal regions. These projections were compared to that of neurons containing the peptides "melanin-concentrating hormone" (MCH) or hypocretin (Hcrt). Because the distribution of these neurons is stereotyped, it was possible to characterize distinct divisions into the LHA. Some of them in the rostral tuberal LHA [the dorsal (LHAd) and suprafornical regions (LHAs)] received a distinct innervation by projections that originated from neurons in respectively anterior or posterior regions of the medial part (CEAm) or from the intermediate part (CEAi) of the central nucleus. Therefore, this work illustrates that projections from the CEAm and CEAi converge into the caudal lateral LHA but diverge into the rostral tuberal LHA.

15.
J Comp Neurol ; 526(14): 2165-2186, 2018 10 01.
Artigo em Inglês | MEDLINE | ID: mdl-29893014

RESUMO

The actual organization of the central nucleus of the amygdala (CEA) in the rat is mostly based on cytoarchitecture and the distribution of several cell types, as described by McDonald in 1982. Four divisions were identified by this author. However, since this original work, one of these divisions, the intermediate part, has not been consistently recognized based on Nissl-stained material. In the present study, we observed that a compact condensation of retrogradely labeled cells is found in the CEA after fluorogold injection in the anterior region of the tuberal lateral hypothalamic area (LHA) in the rat. We then searched for neurochemical markers of this cell condensation and found that it is quite specifically labeled for calbindin (Cb), but also contains calretinin (Cr), tyrosine hydroxylase (TH) and methionine-enkephalin (Met-Enk) immunohistochemical signals. These neurochemical features are specific to this cell group which, therefore, is distinct from the other parts of the CEA. We then performed cholera toxin injections in the mouse LHA to identify this cell group in this species. We found that neurons exist in the medial and rostral CEAl that project into the LHA but they have a less tight organization than in the rat.


Assuntos
Núcleo Central da Amígdala/fisiologia , Animais , Calbindina 2/metabolismo , Calbindinas/metabolismo , Núcleo Central da Amígdala/anatomia & histologia , Encefalina Metionina/metabolismo , Região Hipotalâmica Lateral/anatomia & histologia , Região Hipotalâmica Lateral/fisiologia , Imuno-Histoquímica , Masculino , Camundongos , Vias Neurais/anatomia & histologia , Vias Neurais/metabolismo , Vias Neurais/fisiologia , Neurônios/fisiologia , Ratos , Ratos Sprague-Dawley , Tirosina 3-Mono-Oxigenase/metabolismo
16.
Brain Stimul ; 10(4): 748-756, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28416160

RESUMO

BACKGROUND: Transcranial direct current stimulation (tDCS) is a non-invasive method increasingly popular for the treatment of several brain disorders, such as major depression. Despite great enthusiasm and promising results, some studies report discrepant findings and no consensus exists for the clinical use of tDCS. OBJECTIVE: The present study aims to (i) determine the most effective stimulation parameters to optimize antidepressant-like effect of tDCS in the forced-swim test in mice and (ii) identify brain regions recruited by tDCS and possibly involved in its behavioral effect using Fos immunohistochemistry. RESULTS: We reported that tDCS induced long-lasting antidepressant-like effect, which varied as a function of stimulation settings including number, duration, intensity and polarity of stimulation. Interestingly, the present study also demonstrated that tDCS reduced depressive-like behaviors induced by chronic corticosterone exposure. Furthermore, behavioral outcomes induced by a single stimulation were associated with neuronal activation in the prefrontal cortex, dorsal hippocampus, ventral tegmental area and nucleus accumbens, whereas no overexpression of c-fos was associated with 10 stimulations. CONCLUSION: The strongest behavioral response was observed with an anodal stimulation of 200 µA during 20min. The repetition of this stimulation was necessary to induce long-lasting behavioral effects that are probably associated with plastic changes in the neuronal response.


Assuntos
Transtorno Depressivo Maior/terapia , Estimulação Transcraniana por Corrente Contínua , Animais , Transtorno Depressivo Maior/fisiopatologia , Feminino , Hipocampo/fisiopatologia , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Córtex Pré-Frontal/fisiopatologia , Natação
17.
Brain Struct Funct ; 222(7): 2961-2991, 2017 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-28258483

RESUMO

The parasubthalamic nucleus (PSTN) and the ventrally adjacent calbindin nucleus (CbN) form a nuclear complex in the posterior lateral hypothalamic area (LHA), recently characterized as connected with the central nucleus of the amygdala (CEA). The aim of the present work is to analyze in detail the projections from the amygdala into the PSTN/CbN, also focusing on pathways into the LHA. After fluorogold injections into the PSTN/CbN, the medial part of the CEA (CEAm) appears to be the main supplier of projections from the CEA. Other amygdalar nuclei contribute to the innervation of the PSTN/CbN complex, including the anterior part of the basomedial nucleus (BMAa). Injections of the anterograde tracer, Phaseolus vulgaris leucoagglutinin (PHAL), into the CEAm and BMAa revealed that projections from the CEAm follow two pathways into the LHA: a dorsal pathway formed by axons that also innervate the paraventricular hypothalamic nucleus, the anterior perifornical LHA and the PSTN, and a ventral pathway that runs laterally adjacent to the ventrolateral hypothalamic tract (vlt) and ends in the CbN. By contrast, the BMAa and other telencephalic structures, such as the fundus striatum project to the CbN via the ventral pathway. Confirming the microscopic observation, a semi-quantitative analysis of the density of these projections showed that the PSTN and the CbN are the major hypothalamic targets for the projections from the CEAm and the BMAa, respectively. PSTN and CbN receive these projections through distinct dorsal and ventral routes in the LHA. The ventral pathway forms a differentiated tract, named here the ventrolateral amygdalo-hypothalamic tract (vlah), that is distinct from, but runs adjacent to, the vlt. Both the vlt and the vlah had been previously described as forming an olfactory path into the LHA. These results help to better characterize the CbN within the PSTN/CbN complex and are discussed in terms of the functional organization of the network involving the PSTN and the CbN as well as the CEA and the BMAa.


Assuntos
Complexo Nuclear Basolateral da Amígdala/fisiologia , Mapeamento Encefálico , Calbindinas/metabolismo , Região Hipotalâmica Lateral/fisiologia , Vias Neurais/fisiologia , Neurônios/metabolismo , Animais , Complexo Nuclear Basolateral da Amígdala/citologia , Calbindina 2/metabolismo , Peptídeo Relacionado com Gene de Calcitonina/metabolismo , Região Hipotalâmica Lateral/metabolismo , Masculino , Parvalbuminas/metabolismo , Fito-Hemaglutininas/metabolismo , Ratos , Ratos Sprague-Dawley , Estilbamidinas/metabolismo
18.
J Comp Neurol ; 525(6): 1489-1498, 2017 Apr 15.
Artigo em Inglês | MEDLINE | ID: mdl-27580962

RESUMO

The claustrum is a small, elongated nucleus close to the external capsule and deep in the insular cortex. In rodents, this nucleus is characterized by a dense cluster of parvalbumin labeling. The claustrum is connected with the cerebral cortex. It does not project to the brainstem, but brainstem structures can influence this nucleus. To identify some specific projections from the lateral hypothalamus and midbrain, we analyzed the distribution of projections labeled with antibodies against tyrosine hydroxylase (TH), melanin-concentrating hormone (MCH), and hypocretin (Hcrt) in the region of the claustrum. The claustrum contains a significant projection by MCH axons, whereas it is devoid of TH projections. Unlike TH and MCH axons, Hcrt axons are scattered throughout the region. This observation is discussed mainly with regard to the role of the claustrum in cognitive functions and that of MCH in REM sleep. J. Comp. Neurol. 525:1489-1498, 2017. © 2016 Wiley Periodicals, Inc.


Assuntos
Axônios/metabolismo , Gânglios da Base/citologia , Gânglios da Base/metabolismo , Vias Neurais/citologia , Vias Neurais/metabolismo , Animais , Imunofluorescência , Hormônios Hipotalâmicos/metabolismo , Imageamento Tridimensional , Masculino , Melaninas/metabolismo , Orexinas/metabolismo , Hormônios Hipofisários/metabolismo , Ratos , Ratos Sprague-Dawley , Tirosina 3-Mono-Oxigenase/metabolismo
19.
J Chem Neuroanat ; 75(Pt A): 28-31, 2016 09.
Artigo em Inglês | MEDLINE | ID: mdl-26459022

RESUMO

The LHA contains neurons producing melanin-concentrating hormone (MCH) or hypocretin (Hcrt) that have emerged as being more conspicuous and representative of the posterior LHA. In this review, we focus on MCH neurons and show that they have unique qualities. Their distribution is conserved in the posterior hypothalamus of all vertebrates and their ontogenetic differentiation is very precocious in the rodent embryo. In mammals, interspecific differences in their medio-lateral distribution suggest that the LHA differentiation may follow species specific strategies. These characteristics make a very valuable tool of MCH neurons to study the development as well as the phylogenetical origin and differentiation of the LHA.


Assuntos
Evolução Biológica , Hormônios Hipotalâmicos/metabolismo , Hipotálamo/citologia , Hipotálamo/metabolismo , Melaninas/metabolismo , Neurônios/citologia , Hormônios Hipofisários/metabolismo , Animais , Diferenciação Celular , Humanos , Filogenia
20.
Brain Struct Funct ; 221(4): 2183-208, 2016 05.
Artigo em Inglês | MEDLINE | ID: mdl-25863939

RESUMO

The lateral hypothalamic area (LHA) has two major roles: arousal/waking and food intake controls. Here, it is shown that a premammillary part of the LHA is neurochemically and cytoarchitectonically distinct from the tuberal LHA in male rats. This part contains nuclear masses, namely the parasubthalamic nucleus and the calbindin nucleus, involved in pathways that predict its participation in the control of food intake. Analyzing c-Fos expression in experiments related to feeding behavior, this region responded specifically to the ingestion of palatable nutriments.


Assuntos
Condicionamento Clássico/fisiologia , Comportamento Alimentar/fisiologia , Região Hipotalâmica Lateral/citologia , Região Hipotalâmica Lateral/metabolismo , Animais , Nível de Alerta , Calbindinas/metabolismo , Núcleo Central da Amígdala/citologia , Córtex Cerebral/citologia , Ingestão de Alimentos , Glutamato Descarboxilase/metabolismo , Hormônios Hipotalâmicos/metabolismo , Masculino , Melaninas/metabolismo , Vias Neurais/citologia , Vias Neurais/metabolismo , Orexinas/metabolismo , Parvalbuminas/metabolismo , Hormônios Hipofisários/metabolismo , Proteínas Proto-Oncogênicas c-fos/metabolismo , Ratos , Ratos Sprague-Dawley , Substância P/metabolismo , Tirosina 3-Mono-Oxigenase/metabolismo
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...