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Development ; 148(12)2021 06 15.
Artigo em Inglês | MEDLINE | ID: mdl-34128985

RESUMO

Epithelial attachment to the basement membrane (BM) is essential for mammary gland development, yet the exact roles of specific BM components remain unclear. Here, we show that Laminin α5 (Lama5) expression specifically in the luminal epithelial cells is necessary for normal mammary gland growth during puberty, and for alveologenesis during pregnancy. Lama5 loss in the keratin 8-expressing cells results in reduced frequency and differentiation of hormone receptor expressing (HR+) luminal cells. Consequently, Wnt4-mediated crosstalk between HR+ luminal cells and basal epithelial cells is compromised during gland remodeling, and results in defective epithelial growth. The effects of Lama5 deletion on gland growth and branching can be rescued by Wnt4 supplementation in the in vitro model of branching morphogenesis. Our results reveal a surprising role for BM-protein expression in the luminal mammary epithelial cells, and highlight the function of Lama5 in mammary gland remodeling and luminal differentiation.


Assuntos
Diferenciação Celular/genética , Epitélio/metabolismo , Laminina/genética , Glândulas Mamárias Animais/metabolismo , Transdução de Sinais , Proteína Wnt4/genética , Animais , Biomarcadores , Células Epiteliais , Feminino , Imunofluorescência , Regulação da Expressão Gênica no Desenvolvimento , Imuno-Histoquímica , Laminina/metabolismo , Glândulas Mamárias Animais/embriologia , Camundongos , Modelos Biológicos , Morfogênese/genética , Organogênese/genética , Proteína Wnt4/metabolismo
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