Coevolution of rostrum and brain in pig species.

Domestic pigs have a prominent cortical gyrus (the rostrum gyrus) isomorphic to the contralateral hemirostrum. It is unclear, however, if the size and shape of the rostrum gyrus are of evolutionary/functional relevance. Here, we address this question by assessing the relationship of rostrum and rostrum gyrus across eight pig species. To this end, we quantified rostrum morphology in fresh and alcohol-preserved pig specimens by surface scans, microfocus computed tomography scans, and photography. We establish that the size and shape of the rostrum gyrus can be precisely inferred from endocasts. We then took advantage of the accessibility of pig skulls and endocasts to assess features of the rostrum gyrus across species. Our investigation led to the following results: (i) The rostra of pig species show basic similarities. (ii) A cortical rostrum gyrus is apparent in all pigs. (iii) The size of the rostrum gyrus differs across species and outgroups of the evolutionary dominant suinae (i.e., peccaries and the babirusa) have a noticeably smaller rostrum gyrus. (iv) Warthogs have a derived rostrum morphology with an extra fold and a very wide rostrum; the warthog rostrum gyrus recapitulates these rostrum features. (v) Domestic pigs have relatively smaller rostrum gyrus than wild boars. We also provide indications for a conserved cytoarchitectonic patterning of the rostrum gyrus. We conclude that the rostrum gyrus is a neural module that was putatively present in the common ancestor of pigs and that this neural module is conserved across pig species. Natural selection maintains the rostrum gyrus' size and its exact isomorphism to the rostrum.

An isomorphic three-dimensional cortical model of the pig rostrum.

Physiological studies of the last century mapped a somatosensory cortical gyrus representing the pig's rostrum. Here, we describe the extraordinary correspondence of this gyrus to the rostrum. The pig rostrum is packed with microvibrissae (~470 per hemi-rostrum) and innervated by a prominent infraorbital nerve, containing about 80,000 axons. The pig's rostrum has three major skin-folds. The nostrils have a rectangular medial wall and a funnel-like lateral opening, nasal channels run obliquely from lateral (surface) to medial (inside). The rostrum gyrus mimics rostrum geometry in great detail. The putative representation of skin folds coincides with blood sinus and folds of the rostrum gyrus. The putative nostril representation is an oblique sulcus running from lateral (surface) to medial (inside). As observed in rodents, Layer 4 is thin in the nostril sulcus. The side of the nostril sulcus representing the medial wall of the nostril is rectangular, whereas the side of the nostril sulcus representing the lateral wall is funnel-like. Proportions and geometry of the rostrum and the rostrum gyrus are similar, albeit with a collapsed nostril and a larger interindividual variability in the gyrus. The pig's cortical rostrum gyrus receives dense thalamic innervation, has a thin Layer 1 and contains roughly 8 million neurons. With all that, the rostrum gyrus looks like a model of the pig rostrum at a scale of ~1:2. Our findings are reminiscent of the raccoon cortex with its forepaw-like somatosensory forepaw-representation. Representing highly relevant afferents in three-dimensional body-part-models might facilitate isomorphic cortical computations in large-brained tactile specialists.

Phosphate restriction significantly reduces mortality in uremic rats with established vascular calcification.

The role of hyperphosphatemia in the pathogenesis of secondary hyperparathyroidism, cardiovascular disease, and progression of renal failure is widely known. Here we studied effects of dietary phosphate restriction on mortality and vascular calcification in uremic rats. Control and uremic rats were fed a high-phosphate diet and at 3 months a portion of rats of each group were killed. Serum phosphate and the calcium phosphate product increased in uremic rats, as did aortic calcium. Of the rats, 56% had positive aortic staining for calcium (von Kossa), RUNX2, and osteopontin. The remaining uremic rats were continued on diets containing high phosphate without and with sevelamer, or low phosphate, and after 3 more months they were killed. Serum phosphate was highest in uremic rats on high phosphate. Serum PTH and FGF-23 were markedly lower in rats on low phosphate. Mortality on high phosphate was 71.4%, with sevelamer reducing this to 37.5% and phosphate restriction to 5.9%. Positive aortic staining for von Kossa, RUNX2, and osteopontin was increased, but phosphate restriction inhibited this. Kidneys from low-phosphate and sevelamer-treated uremic rats had less interstitial fibrosis, glomerulosclerosis, and inflammation than those of uremic rats on high phosphate. Importantly, kidneys from rats on low phosphate showed improvement over kidneys from high-phosphate rats at 3 months. Left ventricles from rats on low phosphate had less perivascular fibrosis and smaller cardiomyocyte size compared to rats on high phosphate. Thus, intensive phosphate restriction significantly reduces mortality in uremic rats with severe vascular calcification.