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Commun Biol ; 7(1): 674, 2024 Jun 01.
Artigo em Inglês | MEDLINE | ID: mdl-38824207

RESUMO

Studying cellular mechanoresponses during cancer metastasis is limited by sample variation or complex protocols that current techniques require. Metastasis is governed by mechanotransduction, whereby cells translate external stimuli, such as circulatory fluid shear stress (FSS), into biochemical cues. We present high-throughput, semi-automated methods to expose cells to FSS using the VIAFLO96 multichannel pipetting device custom-fitted with 22 G needles, increasing the maximum FSS 94-fold from the unmodified tips. Specifically, we develop protocols to semi-automatically stain live samples and to fix, permeabilize, and intracellularly process cells for flow cytometry analysis. Our first model system confirmed that the pro-apoptotic effects of TRAIL therapeutics in prostate cancer cells can be enhanced via FSS-induced Piezo1 activation. Our second system implements this multiplex methodology to show that FSS exposure (290 dyn cm-2) increases activation of murine bone marrow-derived dendritic cells. These methodologies greatly improve the mechanobiology workflow, offering a high-throughput, multiplex approach.


Assuntos
Mecanotransdução Celular , Neoplasias da Próstata , Animais , Humanos , Camundongos , Neoplasias da Próstata/patologia , Neoplasias da Próstata/imunologia , Masculino , Células Dendríticas/imunologia , Linhagem Celular Tumoral , Ensaios de Triagem em Larga Escala/métodos , Estresse Mecânico , Ligante Indutor de Apoptose Relacionado a TNF/metabolismo , Citometria de Fluxo/métodos , Canais Iônicos
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