Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 4 de 4
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
Brain Struct Funct ; 223(1): 195-210, 2018 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-28752318

RESUMO

Compared to men, women disproportionally experience alcohol-related organ damage, including brain damage, and while men remain more likely to drink and to drink heavily, there is cause for concern because women are beginning to narrow the gender gap in alcohol use disorders. The hippocampus is a brain region that is particularly vulnerable to alcohol damage, due to cell loss and decreased neurogenesis. In the present study, we examined sex differences in hippocampal damage following binge alcohol. Consistent with our prior findings, we found a significant binge-induced decrement in dentate gyrus (DG) granule neurons in the female DG. However, in the present study, we found no significant decrement in granule neurons in the male DG. We show that the decrease in granule neurons in females is associated with both spatial navigation impairments and decreased expression of trophic support molecules. Finally, we show that post-binge exercise is associated with an increase in trophic support and repopulation of the granule neuron layer in the female hippocampus. We conclude that sex differences in alcohol-induced hippocampal damage are due in part to a paucity of trophic support and plasticity-related signaling in females.


Assuntos
Alcoolismo , Transtornos Cognitivos/etiologia , Hipocampo/patologia , Caracteres Sexuais , Alcoolismo/complicações , Alcoolismo/metabolismo , Alcoolismo/patologia , Animais , Fator Neurotrófico Derivado do Encéfalo/metabolismo , Proteína de Ligação a CREB/metabolismo , Modelos Animais de Doenças , Complexo IV da Cadeia de Transporte de Elétrons/metabolismo , Etanol/toxicidade , Comportamento Exploratório/fisiologia , Feminino , Fluoresceínas/metabolismo , Marcação In Situ das Extremidades Cortadas , Fator de Crescimento Insulin-Like I/metabolismo , Antígeno Ki-67/metabolismo , Masculino , Aprendizagem em Labirinto/fisiologia , Condicionamento Físico Animal , Ratos , Ratos Long-Evans , Navegação Espacial/fisiologia
2.
Brain Res ; 1574: 6-13, 2014 Jul 29.
Artigo em Inglês | MEDLINE | ID: mdl-24949562

RESUMO

Bortezomib is a first generation proteasome inhibitor that is the frontline chemotherapy for multiple myeloma with the chief dose-limiting side effect of painful peripheral neuropathy. The goal of this study was to define the behavioral phenotype in a preclinical model of bortezomib chemotherapy-induced peripheral neuropathy (CIPN) and to test whether this is matched by changes in the physiological responses of spinal wide dynamic range neurons. Sprague-Dawley rats were treated with four injections of bortezomib at four doses, 0.05mg/kg, 0.10mg/kg, 0.15mg/kg, 0.20mg/kg, or equal volume of saline. All doses of bortezomib above 0.05mg/kg produced showed significant dose-dependent mechanical hyperalgesia that was fully established at 30 days after treatment and that recovered to baseline levels by day 69 after treatment. Thermal, cold, and motor testing were all unaffected by treatment with bortezomib. Spinal wide dynamic range (WDR) neurons in rats with confirmed bortzomib-related CIPN showed an increase in number of evoked discharges to mechanical stimuli and exaggerated after-discharges in rats with bortezomib CIPN.


Assuntos
Antineoplásicos/efeitos adversos , Ácidos Borônicos/efeitos adversos , Neurônios/efeitos dos fármacos , Doenças do Sistema Nervoso Periférico/induzido quimicamente , Pirazinas/efeitos adversos , Medula Espinal/efeitos dos fármacos , Potenciais de Ação/efeitos dos fármacos , Potenciais de Ação/fisiologia , Animais , Bortezomib , Modelos Animais de Doenças , Relação Dose-Resposta a Droga , Hiperalgesia/induzido quimicamente , Hiperalgesia/fisiopatologia , Vértebras Lombares , Masculino , Atividade Motora/efeitos dos fármacos , Atividade Motora/fisiologia , Neurônios/fisiologia , Limiar da Dor/efeitos dos fármacos , Limiar da Dor/fisiologia , Doenças do Sistema Nervoso Periférico/fisiopatologia , Estimulação Física , Ratos Sprague-Dawley , Medula Espinal/fisiopatologia , Temperatura , Tato
3.
J Pain ; 14(11): 1255-69, 2013 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-24035349

RESUMO

UNLABELLED: Pain is ultimately a perceptual phenomenon. It is built from information gathered by specialized pain receptors in tissue, modified by spinal and supraspinal mechanisms, and integrated into a discrete sensory experience with an emotional valence in the brain. Because of this, studying intact animals allows the multidimensional nature of pain to be examined. A number of animal models have been developed, reflecting observations that pain phenotypes are mediated by distinct mechanisms. Animal models of pain are designed to mimic distinct clinical diseases to better evaluate underlying mechanisms and potential treatments. Outcome measures are designed to measure multiple parts of the pain experience, including reflexive hyperalgesia measures, sensory and affective dimensions of pain, and impact of pain on function and quality of life. In this review, we discuss the common methods used for inducing each of the pain phenotypes related to clinical pain syndromes as well as the main behavioral tests for assessing pain in each model. PERSPECTIVE: Understanding animal models and outcome measures in animals will assist in translating data from basic science to the clinic.


Assuntos
Modelos Animais de Doenças , Manejo da Dor , Medição da Dor , Dor/fisiopatologia , Animais , Comportamento Animal/fisiologia , Hiperalgesia/fisiopatologia , Hiperalgesia/terapia , Neuralgia/fisiopatologia , Neuralgia/terapia
4.
J Pain ; 14(2): 205-14, 2013 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-23374942

RESUMO

UNLABELLED: Spinal glial cells contribute to the development of many types of inflammatory and neuropathic pain. Here the contribution of spinal astrocytes and astrocyte gap junctions to oxaliplatin-induced mechanical hypersensitivity was explored. The expression of glial fibrillary acidic protein (GFAP) in spinal dorsal horn was significantly increased at day 7 but recovered at day 14 after oxaliplatin treatment, suggesting a transient activation of spinal astrocytes by chemotherapy. Astrocyte-specific gap junction protein connexin 43 (Cx43) was significantly increased in dorsal horn at both day 7 and day 14 following chemotherapy, but neuronal (connexin 36 [Cx36]) and oligodendrocyte (connexin 32 [Cx32]) gap junction proteins did not show any change. Blockade of astrocyte gap junction with carbenoxolone (CBX) prevented oxaliplatin-induced mechanical hypersensitivity in a dose-dependent manner and the increase of spinal GFAP expression, but had no effect once the mechanical hypersensitivity induced by oxaliplatin had fully developed. These results suggest that oxaliplatin chemotherapy induces the activation of spinal astrocytes and this is accompanied by increased expression of astrocyte-astrocyte gap junction connections via Cx43. These alterations in spinal astrocytes appear to contribute to the induction but not the maintenance of oxaliplatin-induced mechanical hypersensitivity. Combined, these results suggest that targeting spinal astrocyte/astrocyte-specific gap junction could be a new therapeutic strategy to prevent oxaliplatin-induced neuropathy. PERSPECTIVE: Spinal astrocytes but not microglia were recently shown to be recruited in paclitaxel-related chemoneuropathy. Here, spinal astrocyte gap junctions are shown to play an important role in the induction of oxaliplatin neuropathy.


Assuntos
Antineoplásicos/toxicidade , Astrócitos/efeitos dos fármacos , Junções Comunicantes/efeitos dos fármacos , Hiperalgesia/induzido quimicamente , Compostos Organoplatínicos/toxicidade , Dor/induzido quimicamente , Medula Espinal/efeitos dos fármacos , Animais , Comportamento Animal/efeitos dos fármacos , Carbenoxolona/farmacologia , Conexina 43/metabolismo , Conexinas/metabolismo , Interpretação Estatística de Dados , Proteína Glial Fibrilar Ácida/biossíntese , Hiperalgesia/fisiopatologia , Hiperalgesia/psicologia , Imuno-Histoquímica , Masculino , Oxaliplatina , Dor/fisiopatologia , Dor/psicologia , Medição da Dor/efeitos dos fármacos , Estimulação Física , Células do Corno Posterior/efeitos dos fármacos , Células do Corno Posterior/metabolismo , Ratos , Ratos Sprague-Dawley , Medula Espinal/citologia
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...