RESUMO
Alarmed by research that reveals Hungary as having one of the lowest reporting rates in cases of sexual violence in Europe, this article provides an overview of the research that explains why, historically, sexual violence has been and continues to be underreported all over the globe, from law enforcement and criminal justice perspective. Furthermore, we describe the unique circumstances that might influence Hungarian victims of sexual violence to make formal reports. Among other possible factors, we discuss rape myth acceptance, victim blaming, feminist activism, institutional betrayal, and media representations of rape. In an effort to provide insight into Hungarian gender politics, this article raises salient theoretical works on gender ideology and gender policy in contemporary Hungary. This article concludes with a discussion on what implications such research in Hungary may have on a global understanding of sexual violence reporting.
Assuntos
Vítimas de Crime , Estupro , Delitos Sexuais , Humanos , Hungria , Aplicação da Lei , FeminismoRESUMO
BACKGROUND: Apple skins are a rich source of flavonols, in particular quercetin (Q) glycosides. The objective of the present study was to investigate the presence of Q metabolites in plasma, various tissues, and excreta when the commercial broiler chicken's diet was supplemented with Q (0, 50, 150, 300, or 600 mg kg(-1) body weight per day), an apple skin extract (ASE; 50, 150 mg total phenolics kg(-1) body weight per day), or a dried apple skin powder (ASP; 50 mg total phenolics kg(-1) body weight per day). RESULTS: When Q was supplemented for 3 days, Q sulfate, Q glucuronide, Q glucoside glucuronide, Q glucoside sulfate, and isorhamnetin glucoside were detected by liquid chromatography-tandem mass spectrometry in the liver and duodenum. Deconjugated Q was also detected in the breast and thigh tissues of ASE- and ASP-supplemented broilers. Regardless of the source or concentration of Q, the antioxidant capacity measured by ferric reducing ability of plasma (FRAP) assay in the plasma and tissues of the broilers did not change significantly. CONCLUSIONS: As far as is known, this is the first report to demonstrate that Q and its glycosides can be absorbed and metabolized by broiler chickens.
Assuntos
Ração Animal , Antioxidantes/metabolismo , Galinhas/metabolismo , Frutas/química , Glicosídeos/farmacocinética , Malus/química , Quercetina/farmacocinética , Animais , Suplementos Nutricionais , Duodeno/metabolismo , Flavonóis/metabolismo , Absorção Intestinal , Fígado/metabolismo , Carne , Distribuição TecidualRESUMO
We developed a quantitative enzyme immunoassay (EIA) for antibody to hepatitis E virus (HEV) by using truncated HEV capsid protein expressed in the baculovirus system to improve seroepidemiology, to contribute to hepatitis E diagnosis, and to enable vaccine evaluations. Five antigen lots were characterized; we used a reference antiserum to standardize antigen potency. We defined Walter Reed antibody units (WR U) with a reference antiserum by using the four-parameter logistic model, established other reference pools as assay standards, and determined the conversion factor: 1 WR U/ml = 0.125 World Health Organization unit (WHO U) per ml. The EIA performed consistently; median intra- and inter-test coefficients of variation were 9 and 12%, respectively. The accurate minimum detection limit with serum diluted 1:1,000 was 5.6 WR U/ml; the test could detect reliably a fourfold antibody change. In six people followed from health to onset of hepatitis E, the geometric mean antibody level rose from 7.1 WR U/ml to 1,924.6 WR U/ml. We used the presence of 56- and 180-kDa bands by Western blotting as a confirmatory test and to define true-negative and -positive serum specimens. A receiver-operating characteristics plot identified 30 WR U/ml as an optimum cut-point (sensitivity, 86%; specificity, 89%). The EIA detected antibody more sensitively than a commercially available test. The EIA was transferred to another laboratory, where four operators matched reference laboratory results for a panel of unknowns. Quantitation of antibody to HEV and confirmation of its specificity by Western blotting make HEV serology more meaningful.