[Glutathione-dependent enzymes and glucose-6-phosphate dehydrogenase of blood in patients with lymphosarcoma (non-Hodgkin's disease)].

Activities of glutathione-dependent enzymes and glucose-6-phosphate dehydrogenase (GPDH) were studied in blood of the patients with lymphosarcoma (LS). The activity of glutathione reductase (GR), glutathione dehydroascorbate reductase (GDAR), gamma-glutamyltranspeptidase (GGT) and G6PDH in plasma, leucocytes, lymphocytes and erythrocytes of peripheral blood in 30 patients (42-56 years) with LS and in 20 healthy have been determined with spectrophotometric methods (Humalyzer 2000 DE). Leucocytes and lymphocytes were separated from blood using Boyum method. Spearmen method used for correlative analysis. The levels of enzymes activity and results of correlative analysis showed an imbalance of antioxidative system defense and metabolic disturbances in patients with LS. The strong functional interrelation was estimated only between GR and G6PDH in the patients' lymphocytes (r=+0,716; p<0.0005). Interrelation was found between the levels of activity of antioxidative enzymes and activity (stage) of pathologic process, this may be used as the additional biochemical test for differential diagnostics of LS and estimation of the cells proliferative activity.

[The state of enzymatic redox system of glutathione in the blood of patients with lymphosarcoma (non-Hodgkin's lymphoma].

Lipid peroxidation (LPO) processes are enhanced and metabolism is disturbed in patients with lymphosarcoma (LS) (non-Hodgkin's lymphoma). The blood enzymatic redox system was analyzed in patients with LS of two types: lymphoblastic LS (LB LS) and prolymphocytic LS (PL LS). The activities of glutathione reductase (GR), glutathione peroxidase (GP), glutathione dehydroascorbate reductase (GDAR), gamma-glutamyl transpeptidase (GGT), and glucose-6-phosphate dehydrogenase (G6PDH) were spectrophotometrically (Humalyzer 2000, DE) determined in the peripheral plasma, white blood cells, lymphocytes, and red blood cells of 32 aged 42-57 years who had LS and 25 healthy individuals. Peripheral blood lymphocytes and leukocytes were obtained by the method developed by A. Böyum. A search for a correlation was made by the Spearman method. The activities of the enzymes and the data of the correlation analysis suggested antioxidant defense system imbalance and metabolic disturbances in patients with LS. Close functional correlations between GR and GP, GR and G6PDH persisted in patients with both types of the disease. Functional relationships between GR and GDAR remained only in patients with PL LS (r = 0.946; p < 0.001). That between GR and GGT was impaired in patients with LS. A correlation between the activity of antioxidant enzymes and the proliferative activity of blood cells was found in patients with LB LS, which may be used as an additional biochemical test in the differential diagnosis of LS.

[Persistent diabetic macular edema--surgical versus laser therapy]. / Edemul macular diabetic persistent: tratament chirurgical versus terapia laser.

PURPOSE: To compare the benefit of pars plana vitrectomy versus conventional laser therapy in eyes with persistent diabetic macular edema. METHODS: In this interventional, comparative study we prospectively evaluate 22 eyes from 11 patients with bilateral, persistent diabetic macular edema. In one eye (study eye), at random, pars plana vitrectomy with internal limiting membrane (ILM) peeling was performed and in fellow eye (control eye) laser photocoagulation was continued. Cases with taut posterior hyaloid and epiretinal membranes were excluded. No dye was used for ILM staining. Cases were followed-up at least 12 months. RESULTS: Resolution of macular edema was noted in 72.70% of vitrectomized eyes as compared to 18,20% of laser treated eyes. Visual acuity increased in 45.45% of study eyes as compared to only 9.09% of control eyes. Significantly increase of vision (from 0.087 to 0.2) was noted only in vitrectomized eyes previously laser-treated less than 3 times. CONCLUSIONS: Pars plana vitrectomy with ILM peeling was effective in reducing persistent diabetic macular edema in most of the cases. Significant functional result was limited by numerous previous laser sessions.

[Acute retinal necrosis syndrome]. / Sindromul de necroza retiniana acuta.

We present the case of a 29 year old patient, mono-ophthalmic, diagnosed with Acute Retinal Necrosis Syndrome. The disease rapidly progressed despite the medical treatment, and the patient developed complex retinal detachment. After repeated surgical procedures, due to the recurrence of intraocular inflammatory process and the development of a complicated cataract, we finally obtained a good and stable functional result.

[Efficiency of air tamponade in prevention of early recurrence of vitreal hemorrhage in diabetic patients with vitrectomy]. / Eficienta endotamponadei cu aer in preventia recurentei precoce a hemoragiei vitreene la diabeticul vitrectomizat.

PURPOSE: To evaluate the efficacy and safety of air endo-tamponade in preventing early recurrence of vitreous hemorrhage in diabetic vitrectomy. MATERIALS AND METHODS: Prospective, consecutive, interventional, non-randomized study including 40 eyes (from 40 patients) with persistent or recurrent vitreous hemorrhage related to proliferative diabetic retinopathy. At the end of vitrectomy 20 cases (study group) were assigned to complete fluid--air exchange while the other 20 cases (control group) were left with BSS Plus. RESULTS: Early postoperative bleeding occurred in 9 out of 40 cases--22.5% (3 cases from the study group--15% and 6 cases from the control group--30%, p < 0.05). There was no statistically significant difference in visual acuity, intraocular pressure or incidence of late-onset hemorrhage recurrence between both groups during 6-month postoperative follow-up. In the study group there was a higher rate of lens opacities appearance. CONCLUSIONS: Air endo-tamponade seems a simple and efficient way of preventing early recurrence of vitreous hemorrhage in diabetic vitrectomy. Higher rate of lens opacities appearance may counteract this advantage thus this technique could be safely and electively used in pseudophakic patients.

RecA protein promotes the regression of stalled replication forks in vitro.

Replication forks are halted by many types of DNA damage. At the site of a leading-strand DNA lesion, forks may stall and leave the lesion in a single-strand gap. Fork regression is the first step in several proposed pathways that permit repair without generating a double-strand break. Using model DNA substrates designed to mimic one of the known structures of a fork stalled at a leading-strand lesion, we show here that RecA protein of Escherichia coli will promote a fork regression reaction in vitro. The regression process exhibits an absolute requirement for ATP hydrolysis and is enhanced when dATP replaces ATP. The reaction is not affected by the inclusion of the RecO and R proteins. We present this reaction as one of several potential RecA protein roles in the repair of stalled and/or collapsed replication forks in bacteria.