Antibody response in patients admitted to the hospital with suspected SARS-CoV-2 infection: results from a multicenter study across Spain.

AIM: To evaluate the serological response against SARS-CoV-2 in a multicenter study representative of the Spanish COVID pandemic. METHODS: IgG and IgM + IgA responses were measured on 1466 samples from 1236 Spanish COVID-19 patients admitted to the hospital, two commercial ELISA kits (Vircell SL, Spain) based on the detection of antibodies against the viral spike protein and nucleoprotein, were used. RESULTS: Approximately half of the patients presented antibodies (56.8% were IgM + IgA positive and 43.0% were IgG positive) as soon as 2 days after the first positive PCR result. Serological test positivity increased with time from the PCR test, and 10 days after the first PCR result, 91.5% and 88.0% of the patients presented IgM + IgA and IgG antibodies, respectively. CONCLUSION: The high values of sensitivity attained in the present study from a relatively early period of time after hospitalization support the use of the evaluated serological assays as supplementary diagnostic tests for the clinical management of COVID-19.

Diagnóstico serológico de los casos de toxoplasmosis congénita / Serologic diagnosis of congenital toxoplasmosis

Objetivo Conocer el diagnóstico serológico de los casos de toxoplasmosis congénita y la prevalencia de anticuerpos frente a toxoplasma en mujeres embarazadas en nuestra área.Método La seroprevalencia se estudió en 68.712 sueros correspondientes a 47.635 mujeres embarazadas pertenecientes al área del Hospital Miguel Servet durante el período 1992–2008. En el laboratorio de Microbiología se determinaron anticuerpos frente a toxoplasma: IgA, IgM, IgG y estudio de avidez.Resultados La seroprevalencia encontrada fue del 31,9%. La incidencia de infección primaria fue del 0,01% en mujeres susceptibles. Quince mujeres desarrollaron seroconversión durante el embarazo, de las que 9 tuvieron niños con toxoplasmosis congénita.Conclusión Los casos de toxoplasmosis congénita se detectaron mediante seroconversión materna durante el embarazo. La IgA fue el marcador serológico más sensible en la detección de la infección congénita en niños (AU)

Objective To investigate the serological diagnosis of congenital toxoplasmosis and the prevalence of antibodies against toxoplasma in pregnant women in our area.MethodToxoplasmosis seroprevalence was analyzed in 68 712 serum samples from 47 635 pregnant women living in the catchment area of Hospital Miguel Servet during the period of 1992 to 2008. Detection of toxoplasma-specific immunoglobulins (IgM, IgA, IgG) and IgG avidity studies were carried out in the microbiology laboratory.Results Toxoplasmosis seroprevalence was 31.9%. The incidence of primary infection was 0.01% in susceptible pregnant women. Fifteen women seroconverted during pregnancy, and 9 of them had infants with congenital toxoplasmosis. Conclusions The cases of congenital toxoplasmosis were detected by maternal seroconversion during pregnancy. IgA was the most sensitive marker for the detection of congenital infection in neonates (AU)

[Serologic diagnosis of congenital toxoplasmosis]. / Diagnóstico serológico de los casos de toxoplasmosis congénita.

OBJECTIVE: To investigate the serological diagnosis of congenital toxoplasmosis and the prevalence of antibodies against toxoplasma in pregnant women in our area. METHOD: Toxoplasmosis seroprevalence was analyzed in 68 712 serum samples from 47 635 pregnant women living in the catchment area of Hospital Miguel Servet during the period of 1992 to 2008. Detection of toxoplasma-specific immunoglobulins (IgM, IgA, IgG) and IgG avidity studies were carried out in the microbiology laboratory. RESULTS: Toxoplasmosis seroprevalence was 31.9%. The incidence of primary infection was 0.01% in susceptible pregnant women. Fifteen women seroconverted during pregnancy, and 9 of them had infants with congenital toxoplasmosis. CONCLUSIONS: The cases of congenital toxoplasmosis were detected by maternal seroconversion during pregnancy. IgA was the most sensitive marker for the detection of congenital infection in neonates.