1.
Braz J Microbiol
; 46(1): 113-6, 2015 Mar.
Artigo
em Inglês
| MEDLINE
| ID: mdl-26221095
RESUMO
A modified colorimetric high-throughput screen based on pH changes combined with an amidase inhibitor capable of distinguishing between nitrilases and nitrile hydratases. This enzymatic screening is based on a binary response and is suitable for the first step of hierarchical screening projects.
Assuntos
Aminoidrolases/análise , Colorimetria/métodos , Ensaios de Triagem em Larga Escala/métodos , Hidroliases/análise , Amidoidrolases/antagonistas & inibidores , Concentração de Íons de Hidrogênio
2.
Braz. j. microbiol
; 46(1): 113-116, 05/2015. tab, graf
Artigo
em Inglês
| LILACS
| ID: lil-748237
RESUMO
A modified colorimetric high-throughput screen based on pH changes combined with an amidase inhibitor capable of distinguishing between nitrilases and nitrile hydratases. This enzymatic screening is based on a binary response and is suitable for the first step of hierarchical screening projects.
