RESUMO
The influence of various concentrations (0.003-8 mg/mL) of N-acetylcysteine on the formation of biofilms by 15 strains of Staphylococcus epidermidis has been studied. A dose-related decrease in biofilm formation was observed, except with the lowest concentrations. The 'slime' index relative to the control was 63%, 55%, 46%, 34%, 26% and 26% in the presence of 0.25, 0.5, 1, 2, 4, and 8 mg/mL of N-acetylcysteine, respectively. These data are statistically significant. The inhibitory effect of 2 mg/mL of N-acetylcysteine on slime formation was also verified by electron microscopy.
Assuntos
Acetilcisteína/farmacologia , Biofilmes/crescimento & desenvolvimento , Staphylococcus epidermidis/efeitos dos fármacos , Aderência Bacteriana/efeitos dos fármacos , Staphylococcus epidermidis/fisiologiaAssuntos
Antibacterianos/farmacologia , Aderência Bacteriana/efeitos dos fármacos , Staphylococcus/fisiologia , Biofilmes , Coagulase/análise , Humanos , Polissacarídeos Bacterianos/fisiologia , Infecções Estafilocócicas/microbiologia , Staphylococcus/efeitos dos fármacos , Staphylococcus/patogenicidade , Staphylococcus epidermidis/fisiologiaRESUMO
The influence of various incubation atmospheres on the growth and slime production of 23 Staphylococcus epidermidis strains was studied. The atmospheres evaluated were aerobiosis (control), anaerobiosis, candle jar, 5% CO2 and 10% CO2. As compared to the aerobic control, growth was 55.7 +/- 19% (p < 0.01) in anaerobic incubation, 113.7 +/- 12% (p < 0.01) in 5% CO2, 112.8 +/- 13% (p < 0.01) in 10% CO2 and 106.4 +/- 7% (p > 0.1) in the candle jar. The slime production in relation to the aerobic control was 20.3 +/- 19% in anaerobiosis (p < 0.01), 22.3 +/- 27% (p < 0.01) in 5% CO2, 29.4 +/- 39% (p < 0.01) in 10% CO2 and 68.3 +/- 26% (p > 0.1) in the candle jar. The results of this study may explain the discrepancies which have been noted on occasion between slime formation data and pathogenicity.
