Correlation Between Demographic Variables and Complications in Nonsyndromic Craniosynostosis.

PURPOSE: This retrospective cross-sectional, observational study aims to determine the correlation between demographic variables and nonsyndromic craniosynostosis. METHODS: A cross-sectional study was carried out. The inclusion criteria were nonsyndromic craniosynostosis and the exclusion criteria were patients who did not have complete records. RESULTS: The sample included 49 patients and mainly women. Patients with more than 4 days in the intensive care unit were related with more complications. Intracranial Hypertension was seen predominantly in patients between 6 and 8 months P =0007. CONCLUSION: Some variables can modificate complications risk but future studies are needed to further investigate the influence of such variables on craniofacial care.

The Morpho-Functional Three-Dimensional Analysis for Zygomatic Implants: A Clinical Tool With Surgical Implications.

ABSTRACT: Zygomatic implants (ZIs) have been used successfully for the rehabilitation of jaws with severe atrophy for the past 2 decades. The development of computed tomography, three-dimensional (3D) analysis software, and stereolithographic models has positively impacted the development of preoperative planning. This article describes the protocol developed by the Department of Oral and Maxillofacial Surgery of El Bosque University, Bogotá, Colombia, through 10 years of experience, for the installation of ZIs, covering from the times when the implants were placed through intraoperative guidance, until now, in which drilling guides developed by computer-aided design and computer-aided manufacturing are used, without neglecting in their design multiple factors that must be considered. To date, this protocol for the treatment of patients with atrophic jaws through ZIs includes a detailed clinical examination, in which variables such as bone and dental relationship between the jaws, oral opening and dynamic interaction between soft cervicofacial tissues are considered. It also includes a 3D computed tomography planning for the design and preparation of surgical guides whose insertion patterns must be executable during surgery. Together, clinical and imaging analysis converge in what it has been called morpho-functional 3D planning.

El potencial terapéutico del secretoma de las células troncales / The therapeutic potential of stem cells secretome

Resumen Las células madre -o troncales- mesenquimales (CTMs) juegan un papel importante en medicina regenerativa e ingeniería tisular, puesto que estas células indiferenciadas tienen la capacidad de autorrenovarse y de diferenciarse en varios linajes celulares adultos con funciones especializadas diferentes. Estas células cuentan con la capacidad de inmunomodulación y establecimiento celular sin riesgo de generar teratomas, en contraste con las células troncales embrionarias o las células troncales pluripotenciales inducidas. Varios autores también resaltan la capacidad regenerativa de los productos de estas células, conocido como secretoma. Estos productos juegan un papel muy importante en procesos de cicatrización y angiogénesis, por lo que resultan ideales para aplicarlos en terapias basadas en regeneración y reparación de tejidos y órganos. La presente revisión hace un recuento del estado del arte sobre los procesos de inmunomodulación producidos a partir de estos productos celulares.

Abstract Mesenchymal stem cells (MSC's) play an important role in regenerative medicine and tissue engineering, since these undifferentiated cells have the ability to self-renew and differentiate into several adult cell lineages with different, specialized functions. These cells have immunomodulation and homing capacities without the risk of generating teratomas, in contrast to embryonic stem cells or induced pluripotent stem cells. Several authors also highlight the regenerative capacity of products of these cells, known as secretome. These products play a very important role in healing processes and angiogenesis, making them ideal for application in therapies based on regeneration and repair of tissues and organs. The present review makes a recount of the state of the art on the processes of immodulation produced from these cell products.

Evaluación in vitro de la adhesión de células troncales mesenquimales a matrices dentales impresas en tercera dimensión / In vitro Evaluation of the Mesenchymal Stem Cell Adhesion to 3D-Printed Dental Matrices

Antecedentes: En ingeniería de tejidos es fundamental estudiar el sinergismo entre las células troncales mesenquimales y el biomaterial para tener un mayor control sobre los biomiméticos. De esto depende el éxito de tratamientos de lesiones de tamaño crítico.Objetivo: Evaluar la adhesión celular in vitro de células troncales de la pulpa dental humana (CTPD) en matrices impresas con ácido poliláctico {API.).Métodos: Se utilizaron muestras de CTPD criopreservadas y expandidas, cultivadas sobre 24 matrices dentales impresas en 3D con APL, y que se analizaron los días 1, 7 y 15. Se evaluó la fenotipificacion de la CTPD por citometría de flujo y la adhesión celular a la matriz por medio de microscopio electrónico de barrido (SEM). Los datos se agruparon en porcentajes, tanto para el marcador analizado como para la cantidad de células adheridas. Resultados: Las CTPD expresaron positivamente anticuerpos CD73 y CD90 en casi un 100 % y CD 105 en un 56,7 %. Asimismo, expresaron negativamente anticuerpos CD34 y CD45 en más del 98 %. Se observó en SEM que a los 15 días el 99,88 % de las CTPD presentó forma fusiforme o estrellada, lo que significa que estas células se adhirieron a la matriz de APP.Conclusión : El APL no es citotóxico para las CTPD por su composición y características biocompatibles, lo que hizo posible que las células se adhirieran y proliferaran sobre la matriz dental impresa en 3D. Este fue un método in vitro efectivo para emplear en futuros estudios de regeneración de tejidos en odontología.

Background: In tissue engineering it is quite important to study the synergy between mesenchymal stem cells and biomaterials to better control the biomimetic elements. Success in the treatment of critical-size lesions depends strongly on this fact. Objective: To evaluate the in vitro human Dental Pulpal Stem Cell (DPSC) adhesion in printed matrices developed with polylactic acid (PLA). Methods'. Cryopreserved and enlarged DPSC samples were used in the culture of 24 dental 3D-printed matrices developed with PLA that were analyzed on days 1, 7 and 15. The DPSC typification was analyzed with flow cytometry and the cell adhesion to the matrix was analyzed under the scanning electron microscope (SEM). Data were gathered as percentages for both the analyzed marker and the amounts of adhered cells. Results: DPSCs expressed positively the CD73 and CD90 antibodies in almost 100%and the CD 105 in 56.7%. Likewise, DPSCs expressed negatively the CD34 and CD45 in more than 98%. After 15 days it was observed in the SEM that 99.88% of the DPSCs had either a star-like or fusiform shape, which means that they adhered successfully to the PLA matrix. Conclusion: The PLA is not cytotoxic on the DPSCs thanks to its composition and biocompatible features, which allowed the cell adhesion and proliferation in the 3D-printed dental matrix. This in vitro method was effective and should be use in future studies on tissue regeneration in dentistry.