Espessura corneana mensurada pelo paquímetro ultra-sônico e pelo microscópio especular Topcon SP-2000P em gatos / Corneal thickness measured by ultrasound pachymeter and by Topcon SP-2000P specular microscope Topcon SP-2000P in cats

Avaliou-se a espessura corneana central de 13 gatos normais com microscópio especular de não-contato Topcon SP-2000P e paquímetro ultra-sônico. Foi observado aumento da espessura corneana mensurada pelo paquímetro ultra-sônico comparado ao microscópio especular.

The central corneal thickness of 13 normal cats was evaluated by a Topcon SP-2000P noncontact specular microscope and by an ultrasonic pachymetry was evaluated. An increase on the corneal thickness measured by ultrasonic pachymetry as compared to the specular microscope was observed.

Transplante de membrana amniótica canina criopreservada para cicatrização de córnea com deficiência de células límbicas em coelhos / Transplantation of cryopreserved canine amniotic membrane for cicatrisation in cornea with limbal stem cells deficiency in rabbits

Avaliaram-se as alterações relacionadas à deficiência das células límbicas precursoras do epitélio corneano de coelhos e o efeito da membrana amniótica sobre sua cicatrização. A lesão, induzida com n-heptanol associado à peritomia conjuntival em 360º, foi recoberta com membrana amniótica canina, suturada à episclera perilímbica, criopreservada em meio para congelação de embrião ou em meio próprio, ambos com glicerol a 50 por cento e mantida a -80ºC. O grupo-controle não foi tratado com a membrana. As avaliações histológicas foram realizadas ao sétimo, 15º e 30º dias. Todos desenvolveram deficiência de células germinativas do limbo, denominada conjuntivalização, com presença de neovascularização, inflamação e defeitos epiteliais recorrentes, caracterizada na histopatologia pela presença de neovasos, edema, leucócitos e células caliciformes. O transplante de membrana amniótica não foi eficiente para o tratamento desta deficiência, entretanto auxiliou o processo de cicatrização da córnea

Changes related to limbal stem cells deficiency in corneal epithelium in rabbits, as well as the results of amniotic membrane transplant on the cicatrisation were evaluated. The ulcer was induced with n-heptanol associated to 360º conjunctival peritomy; the corneal surface was covered with canine amniotic membrane, sutured to perilimbal episclera, cryopreserved in embryo solution or own medium, both with 50 percent glycerol and stored at -80ºC. The control group was not treated with membrane. Histological evaluations were performed at seven, 15, and 30 days. All of them developed limbal stem cells deficiency, named conjunctivalization, with neovascularization, inflammation and recurrent epithelial defects, observed in histopathology by the occurrence of neovascularization, edema, leukocytes and goblet cells. Thus amniotic membrane transplantation was not efficient in the treatment of limbal stem cells deficiency, however it helped in the process of cicatrisation

Effects of alpha and beta adrenolytic agents on the changes in arterial pressure induced by intrahypothalamic injection of carbachol and nicotine.

In order to elucidate some aspects of cholinergic and adrenergic interaction in the dorsomedial nucleus of the hypothalamus and its effect on systemic arterial pressure, carbachol and nicotine were injected into this area alone or preceded by regitine and propranolol. Both carbachol and nicotine induced hypotensive effects in normotensive anesthetized rats. Previous application of 20, 40, 80 and 160 nmol propranolol did not alter the hypotension induced, but doses of 20, 40 and 80 nmol regitine gradually attenuated the hypotensive effects of carbachol and nicotine.