Prevalence and Antimicrobial Resistance of Salmonella spp. in Aquacultured Nile Tilapia (Oreochromis niloticus) Commercialized in Federal District, Brazil.

This study aimed to assess Salmonella spp. prevalence in aquaculture Nile tilapia commercialized in the Federal District, Brazil, and determine the antimicrobial resistance profile of the isolates. Fifty-seven Salmonella spp. strains were isolated from 101 samples of fresh tilapia fillets collected in the Federal District, Brazil. These isolates were subjected to antimicrobial susceptibility testing by the Kirby-Bauer disk diffusion method and analyzed for the presence of blaCTX, tetB, sul2, and floR resistance genes. The Salmonella spp. prevalence in fresh tilapia fillets was 45.5%; that is, 46 of 101 samples were positive for the InvA gene. The antimicrobial resistance profile showed high resistance rates for amoxicillin/clavulanic acid (87.7%), tetracycline (82.5%), sulfonamide (57.9%), and chloramphenicol (26.3%). Additionally, 56.1% of Salmonella spp. isolates were multidrug-resistant (MDR) isolates. The beta-lactam-resistant gene blaCTX was identified in 66.7% of isolates, the tetracycline resistance gene tetA in 54.4%, and the chloramphenicol resistance gene floR in 50.9%, while the sulfonamide resistance gene sul2 was present in 49.1%. The results revealed that tilapia fillets were highly contaminated with MDR Salmonella. These Salmonella spp. strains carried multiple antimicrobial resistance genes, which might facilitate their dissemination to consumers along the production chain. Hence, there is an evident need to control Salmonella in fish production systems to ensure public health.

Perfil de sono, variáveis clínicas e jornada de trabalho de caminhoneiros idosos e de meia-idade em rodovias / Sleep profile, clinical variables, and working hours in elderly and middle-aged long-haul truck drivers

Este trabalho objetivou investigar a associação de variáveis representativas do perfil sociodemográfico, da jornada de trabalho e das condições gerais de saúde de profissionais do transporte de cargas em rodovias com o regime de sono relatado. Tratou-se de um estudo transversal com análise de questionários cedidos pelo Serviço Social do Transporte e pelo Serviço Nacional de Aprendizagem do Transporte, em que foram averiguados dados relativos aos profissionais com idade ≥ 45 anos. Os resultados encontrados permitem inferir que o perfil de sono exibido pelos profissionais de transporte de cargas parece correlacionar-se negativamente com a condição geral de saúde (ocorrência de hipertensão arterial sistêmica e consumo de medicamentos) e a extensão da jornada de trabalho.

This cross-sectional study aimed to investigate the association of variables representative of the sociodemographic profile, working hours, and general health status of long-haul truck drivers with their reported sleep regimen. Questionnaires provided by the Transportation Social Service and the National Transportation Learning Service, in which data were collected from professional truckers aged ≥ 45 years, were analyzed. The results found allow us to infer that the sleep profile of these ground freight transportation workers seems to correlate negatively with general health (prevalence of arterial hypertension and drug use) and the number of working hours per day.