Merging 3D-printing technology and disposable materials for electrochemical purposes: A sustainable alternative to ensure greener electroanalysis.

3D-printing technology has revolutionized electrochemical applications by enabling rapid prototyping of various devices with high precision, even in highly complex structures. However, a significant challenge remains in developing less costly and more sustainable analytical approaches and methods aimed at mitigating the negative environmental impacts of chemical analysis procedures. In this study, we propose a solution to these challenges by creating a simple and versatile electrochemical system that combines 3D-printing technology with recyclable disposable materials, such as graphite from an exhausted battery and a stainless-steel screw. Our results demonstrate a novel strategy for developing electrodes and other laboratory-made devices that align with the principles of sustainability and green chemistry. Furthermore, we provide evidence of the effectiveness of the proposed system in an analytical application involving the simultaneous determination of tert-butylhydroquinone, acetaminophen, and levofloxacin using the voltammetric technique in lake and groundwater samples. The results indicate sufficient accuracy, with recovery values ranging from 91 to 110%. Additionally, we utilized the Analytical GREEnness calculator as a metric system to evaluate the environmental friendliness of the proposed electroanalytical protocol. The final score confirms a favorable level of sustainability, reaffirming the eco-friendly nature of our approach.

The Variation of the Patellar Height in Total Knee Arthroplasties and its Relationship with the Replacement or not of the Patella.

Objective To compare the measurement of patellar height in the pre- and postoperative period of total knee arthroplasties (TKAs) and its variation in patients with and without patellar replacement. Methods Retrospective evaluation of radiographs of patients submitted to TKA between 2014 and 2020. Profile radiographs were evaluated using the modified Caton-Deschamps patellar height index, comparing the pre- and postoperative measurements of 90 patients, with a total of 100 knees. Next, two groups were compared with a different surgical technique, considering the replacement or not of the patella. All patients who had x-rays evaluated had indication of TKA by osteoarthrosis without previous procedures that could interfere at patellar height. Results The statistical analysis showed a statistically significant difference, with the preoperative index superior to the postoperative rate, evidencing an overall decrease in patellar height. The Caton-Deschamps index modified for mean preoperative TKA was 1.41 (±0.25), and it was 1.31 (± 0.25), p < 0.001for postoperative TKA. No significant difference was found in the variation of this index when comparing the groups with and without patellar replacement. The mean difference of the index in the group without patella was 0.11, and 0.08 in the group with patella, and this difference was considered nonsignificant, p = 0.510. Conclusion We can conclude that patellar height had significant variation in the total group, with reduction of patellar height in the postoperative period. However, the height did not vary significantly between the postoperative groups with and without patellar replacement, regardless of the choice of the surgeon.

The Variation of the Patellar Height in Total Knee Arthroplasties and its Relationship with the Replacement or not of the Patella / Variação da altura patelar nas artroplastias totais do joelho e sua relação com a substituição ou não da patela

Abstract Objective To compare the measurement of patellar height in the pre- and postoperative period of total knee arthroplasties (TKAs) and its variation in patients with and without patellar replacement. Methods Retrospective evaluation of radiographs of patients submitted to TKA between 2014 and 2020. Profile radiographs were evaluated using the modified Caton-Deschamps patellar height index, comparing the pre- and postoperative measurements of 90 patients, with a total of 100 knees. Next, two groups were compared with a different surgical technique, considering the replacement or not of the patella. All patients who had x-rays evaluated had indication of TKA by osteoarthrosis without previous procedures that could interfere at patellar height. Results The statistical analysis showed a statistically significant difference, with the preoperative index superior to the postoperative rate, evidencing an overall decrease in patellar height. The Caton-Deschamps index modified for mean preoperative TKA was 1.41 (±0.25), and it was 1.31 (± 0.25), p< 0.001for postoperative TKA. No significant difference was found in the variation of this index when comparing the groups with and without patellar replacement. The mean difference of the index in the group without patella was 0.11, and 0.08 in the group with patella, and this difference was considered nonsignificant, p= 0.510. Conclusion We can conclude that patellar height had significant variation in the total group, with reduction of patellar height in the postoperative period. However, the height did not vary significantly between the postoperative groups with and without patellar replacement, regardless of the choice of the surgeon.

Resumo Objetivo Comparar a medida da altura patelar no pré- e pós-operatório das artroplastias totais do joelho (ATJs) e sua variação nos pacientes com e sem substituição patelar. Métodos Avaliação retrospectiva de radiografias de pacientes submetidos a ATJ entre 2014 e 2020. Foi feita a avaliação de radiografias em perfil, usando o índice de altura patelar de Caton-Deschamps modificado, comparando as medidas do pré- e pós-operatório de 90 pacientes, totalizando 100 joelhos. A seguir, foi feita a comparação de dois grupos, com técnica cirúrgica distinta, considerando a substituição ou não da patela. Todos os pacientes que tiveram radiografias avaliadas tiveram indicação de ATJ por osteoartrose sem procedimentos prévios que pudessem interferir na altura patelar. Resultados A análise estatística demonstrou uma diferença estatisticamente significativa, sendo o índice pré-operatório superior ao pós-operatório; evidenciando um abaixamento global da altura patelar. O índice Caton-Deschamps modificado para ATJ pré-operatório médio foi de 1,41 (±0,25), e o pós foi de 1,31 (±0,25), p< 0,001. Não foi encontrada diferença significativa na variação deste índice quando comparados os grupos com e sem substituição patelar. A diferença média do índice no grupo sem patela foi de 0,11 e no grupo com patela foi de 0,08, sendo esta diferença considerada não significativa, p= 0,510. Conclusão Podemos concluir que a altura patelar teve variação significativa no grupo total, com redução da altura patelar no pós-operatório. Entretanto, a altura não variou de forma significativa entre os grupos pós-operatórios com e sem substituição patelar, independentemente da opção do cirurgião.

Can anti-PGL-I antibody isotypes differentiate leprosy contacts and leprosy patients?

BACKGROUND: Serological tests for antibody measurement in leprosy have a series of limitations in discriminating contacts and patients. The present paper intends to evaluate if association of more than one antibody isotype in serum samples may be a useful tool in leprosy diagnosis. METHODS: This study evaluated 395 leprosy contacts and 71 leprosy index cases living in endemic municipalities in Northeastern Brazil. The participants were evaluated according to their anti-phenolic glycolipid antigen-I isotype (PGL-I) profile. Serum anti-PGL-I IgM, IgG, and IgA were measured by indirect ELISA. RESULTS: A strong association was found for antibody positivity in MB leprosy index cases. The odds ratios were 6.11 (95% CI 3.08 - 12.16) for IgM, 3.31 (1.66 - 6.61) for IgG, and 16.97 (8.39 - 34.2) for IgA. For IgM associated with one or more isotypes, the OR was 21.0 (95% CI 10.11 - 43.64), and for IgG + IgA, the OR was 17.58 (6.23 - 49.54). The highest diagnostic sensitivity of 76.0% (95% CI 61.8 - 86.9) was observed for IgM, and the lowest value was 24.1% (13.0 - 38.2), which was observed for IgG + IgA isotypes. Regarding presumptive positive predictive values, the lowest value was obtained for IgM at 24.7% (95% CI 18.1 - 32.3), and the highest values were observed for IgM+ one or more isotypes and for IgG + IgA isotype at 60.0% (44.3 - 74.3) and 66.7% (41.0 - 86.7), respectively. CONCLUSIONS: The present work demonstrated that by associating two or more positive antibody isotypes, the risk of facing a real case of leprosy may increase.

O impacto da ansiedade em informação nas Startups de Inovação / La información como un recurso de ansiedad en las organizaciones / Information as a source of anxiety in organizations

O presente trabalho tem como objetivo identificar o impacto da informação no contexto da construção de conhecimento em trabalhadores de startups. Nesse sentido, ao observarmos o atual cenário das organizações, analisamos que a informação é capaz de modificar o comportamento dos sujeitos de diferentes maneiras. Dessa forma, ao mesmo tempo em que devemos considerar a informação como um recurso para as organizações, também é necessário compreender o seu impacto na saúde geral dos trabalhadores, que vai desde a sensação de incapacidade em absorver múltiplas informações na produção de conhecimento, entre outros problemas (doenças somáticas, síndrome de esgotamento etc.), o que interfere na saúde mental, tanto quanto à saúde física. O uso da informação provoca inúmeros desencadeamentos nas organizações, todavia, destacamos um comportamento desenvolvido pelos trabalhadores que necessita ser analisado: a ansiedade informacional. Diante desse cenário, a presente pesquisa buscou compreender o comportamento informacional dos trabalhadores do conhecimento nas startups. Para tanto, foi escolhido o método 'estudo de caso', aplicando-se a triangulação, ou seja, aplicando diferentes técnicas de coleta e análise de dados, tornando a pesquisa mais consistente, validando assim os dados e análises realizadas. A população da pesquisa consiste em 18 sujeitos de 4 diferentes empresas de tecnologia da informação que foram enquadradas como startups. Com as informações colhidas, foi possível construir parâmetros baseados em estratégias de Gestão de Pessoas de Ribeiro et al (2019) capazes de minimizar o impacto da ansiedade em informação nos trabalhadores dessas startups(AU)

Este documento tiene como objetivo identificar el impacto de la información en el contexto de la creación de conocimiento en los trabajadores de nuevas empresas. En este sentido, al observar el escenario actual de las organizaciones, analizamos que la información puede modificar el comportamiento de los sujetos de diferentes maneras. Por lo tanto, si bien debemos considerar la información como un recurso para las organizaciones, también es necesario comprender su impacto en la salud general de los trabajadores desde la sensación de incapacidad para absorber información múltiple en la producción de conocimiento; entre otros problemas, enfermedades somáticas, síndrome de agotamiento, etcétera, que interfieren con la salud mental y la salud física. El uso de la información causa numerosos factores desencadenantes en las organizaciones; sin embargo, destacamos un comportamiento desarrollado por los trabajadores que debe analizarse: la ansiedad informativa. Dado este escenario, la presente investigación buscó comprender el comportamiento informativo de los trabajadores del conocimiento en las nuevas empresas. Para esto, se eligió el método "estudio de caso", aplicando la triangulación, es decir, diferentes técnicas de recolección y análisis de datos, haciendo que la investigación sea más consistente, para validar los datos y el análisis realizado. La población de la investigación consistió en 18 sujetos de 4 compañías diferentes de tecnología de la información que fueron clasificadas como startups. Con la información recopilada, fue posible construir parámetros basados ​​en las estrategias de gestión de personas de Ribeiro y otros (2019), capaces de minimizar el impacto de la ansiedad por la información en los trabajadores de estas nuevas empresas(AU)

The purpose of the study was to identify the impact of information in the context of knowledge creation among workers from startup enterprises. Based on observation of the current scenario of organizations, we analyzed the fact that information may modify the behavior of subjects in different ways. Thus, while we should consider information as a resource for organizations, we should also understand its impact on workers' general health in terms of a feeling of inability to grasp multiple information in the process of knowledge production. Among other problems, somatic diseases and the burnout syndrome interfere with mental and physical health. Given this scenario, the study aimed to understand the information behavior of knowledge workers in the new enterprises. To achieve this goal, the case study method was applied, which included the use of triangulation, i.e. different data collection and analysis techniques contributing greater consistency to the study when validating the data and the analysis performed. The study population was 18 subjects from 4 different information technology companies classified as startups. With the information collected, parameters could be constructed on the basis of the people management strategies developed by Ribeiro et al (2019), capable as they are of minimizing the impact of information anxiety among workers from these new enterprises(AU)

Astronium fraxinifolium Schott Exerts Leishmanicidal Activity by Providing a Classically Polarized Profile in Infected Macrophages.

BACKGROUND: Leishmania braziliensis is prevalent in Latin American countries, including Brazil. It causes cutaneous and mucocutaneous leishmaniasis, leading to high morbidity, and has a low cure rate. Treatment is based on pentavalent antimonials; nonetheless, there are problems related to high toxicity, high cost, and parasitic resistance. Discovery of new leishmanicidal drugs without these limitations and that stimulate the cellular immune response is necessary. PURPOSE: The present work evaluates whether Astronium fraxinifolium Schott exerts leishmanicidal activity against L. braziliensis by providing a classically polarized profile in infected macrophages. METHODS: For the evaluation of the A. fraxinifolium Schott leishmanicidal activity, amastigote cell death was demonstrated in infected RAW 267.4 macrophages treated with an ethanolic extract from the plant sapwood (EEAF). For the evaluation of the EEAF capacity in providing a classically polarized profile in infected macrophages, the following analyses were done: detection of LAMP-1 protein by the baculovirus technology, measurement of superoxide anion by the NBT testing, quantification of TNF-α, IL-12p40, IL-10, IL-4, and TGF-ß by sandwich-type enzyme immune assays, and iNOS and COX-2 expression by RT-PCR technique. RESULTS: The EEAF significantly reduced amastigote counts inside the cells. Vacuoles were visualized in infected and treated cells before and after May-Grünwald-Giemsa staining. A strong LAMP-1 protein fluorescence revealed phagosome maturation in infected cells treated with the EEAF. No production of superoxide was visualized in infected cells treated with the plant material. Nonetheless, high levels of TNF-α, IL-12p40, and IL-10 were found in cell supernatants, but reduced levels of TGF-ß and no IL-4 production. We identified augmented mRNA expression for COX-2, but no expression of iNOS mRNA. CONCLUSION: Our results demonstrated that A. fraxinifolium induced a classically polarized profile in infected macrophages but also provided a less harmful environment by stimulating the production of certain anti-inflammatory mediators, such as IL-10.

Antioxidant, anti-inflammatory and healing potential of ethyl acetate fraction of Bauhinia ungulata L. (Fabaceae) on in vitro and in vivo wound model.

The present work aimed to investigate the antioxidant, anti-inflammatory and wound healing potential of ethyl acetate fraction from Bauhinia ungulata L. (FABU) on in vitro and in vivo models. Wound healing assay using human lung adenocarcinoma A549 cell line was employed to evaluate the ability of FABU in modulating cell migration. In addition, a surgical wound model in C57BL/6 mice was used to study the healing potential of FABU incorporated into gel carbomer 940 (Carbopol®). Evaluation of lipid peroxidation, inflammatory and anti-inflammatory mediator gene expression, rate of wound closure, and histological analysis were done. FABU significantly reduced the gap area in in vitro wound healing assay, 24 h after treatment. In the animal model, FABU at 0.5% topically applied once-daily for 5 days to the surgical wounds significantly reduced the lesion area. Moreover, it significantly decreased the levels of lipid peroxidation in the lesions and decreased the relative gene expression levels of IL-1ß and TNF-α in the injured region. In conclusion, our study suggests that Bauhinia ungulata can effectively promote the wound healing, probably by regulating the inflammatory environment during the early stages of the process.

Nitro-imidazole-based ruthenium complexes with antioxidant and anti-inflammatory activities.

Inflammation is a physiological process triggered in response to tissue damage, and involves events related to cell recruitment, cytokines release and reactive oxygen species (ROS) production. Failing to control the process duration lead to chronification and may be associated with the development of various pathologies, including autoimmune diseases and cancer. Considering the pharmacological potential of metal-based compounds, two new ruthenium complexes were synthesized: cis-[Ru(NO2)(bpy)2(5NIM)]PF6 (1) and cis-[RuCl(bpy)2(MTZ)]PF6 (2), where bpy = 2,2'-bipyridine, 5NIM = 5-nitroimidazole and MTZ = metronidazole. Both products were characterized by spectroscopic techniques, followed by Density Functional Theory (DFT) calculations in order to support experimental findings. Afterwards, their in vitro cytotoxic, antioxidant and anti-inflammatory activities were investigated. Compounds 1 and 2 presented expressive in vitro antioxidant activity, reducing lipid peroxidation and decreasing intracellular ROS levels with comparable effectiveness to the standard steroidal drug dexamethasone or α-tocopherol. These complexes showed no noticeable cytotoxicity on the tested cancer cell lines. Bactericidal assay against metronidazole-resistant Helicobacter pylori, a microorganism able to disrupt oxidative balance, unraveled compound 1 moderate activity over that strain. Besides this, it was able to inhibit interleukin-6 (IL-6) and tumor necrosis factor-α (TNF- α) production as well as interleukin-1ß (IL-1ß) and cyclooxygenase-2 (COX-2) expression in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages. This latter activity is remarkable, which has not been reported for other ruthenium-based complexes. Altogether, these results suggest cis-[Ru(NO2)(bpy)2(5NIM)]PF6 complex has potential pharmacological application as an anti-inflammatory agent that deserve further biological investigation.

Interleukin-18 promoter -137 G/C polymorphism (rs187238) is associated with biochemical markers of renal function and cardiovascular disease in type 2 diabetes patients.

BACKGROUND: Interleukin-18 (IL-18), a proinflammatory and proatherogenic cytokine, has been associated with type 2 diabetes, metabolic syndrome, stroke and coronary artery disease. Some studies have indicated that the IL-18 promoter -137 G/C polymorphism seems to be associated with changes in the IL-18 expression and may contribute to the development of cardiovascular disease (CVD). The aim of this study was to evaluate the association between -137 G/C polymorphism and the levels of IL-18, biochemical markers for cardiovascular disorders, anthropometric profile and cardiovascular disease in Brazilian patients with type 2 diabetes (T2DM). DESIGN & METHODS: Study subjects comprised 125 T2DM patients undergoing follow-up at a reference endocrinology service in northeastern Brazil. The -137G/C polymorphism in the IL-18 gene and serum IL-18 levels were determined by using allele-specific polymerase chain reaction (PCR) and enzyme-linked immune assay (ELISA), respectively. The anthropometric parameters were assessed using a Body Composition Monitor with Scale, and the laboratory data were measured using an automatic analyzer as well as spectrophotometric analysis. RESULTS: The genotype distribution of IL-18 -137 G/C genetic polymorphism was significantly different among T2DM patients with and without CVD. The results show an association between the CC genotype of -137G/C polymorphism and CVD in T2DM patients (p < 0.001). Serum levels of IL-18 were significantly higher in CC carriers (843.1 pg/mL) compared with GG or GC carriers (303.6 pg/mL and 292.0 pg/mL, respectively). In addition, the present study showed that carriers of the CC genotype also had significantly higher concentrations of creatinine and albuminuria than carriers of the GG or GC genotypes (p < 0.05 in both). CONCLUSION: These results suggest that Brazilian T2DM patients with the CC genotype seem to show a predisposition to CVD, as well as an elevation in markers of renal function.

Dataset and detailed methodology for structure and performance characterization of modified polymeric membranes.

The data contained in this publication refers to protocols adopted characterization of clay nanoparticles (CN) membranes with and without the use of polyethylene oxide (PEO) as pore former. The membrane casting solutions were produced by dissolving PS (18% w/w) in NMP with addition of CN (1-5% w/w CN/PS) and/or PEO (1-5% w/w PEO/PS) when applicable. Membranes with no CN or PEO were used as a control. Pure water permeability of cast membranes was determined using the cross-flow cell unit. Viscosity was measured for most casting solution compositions and contact angle was measured for all membranes. The control membrane was further compared in detail to the highest permeability membranes with only CN (1.5%), only PEO (5%), 1.5% CN and 5% PEO (combination of optimal individual permeabilities), and 4.5% CN and 5% PEO (optimal combined permeability) regarding thickness, porosity, rejection, fouling resistance, surface charge, and thermal/mechanical properties. The relevance of the data presented here is to show details about methods for characterizing membranes for future comparison of performance and eventual improvement of characterization methods.

Serum Anti-PGL-1 IgG, IgM, and IgA in a 3-Year Follow-up Study of 4-15-Year-old Leprosy Contacts.

BACKGROUND: In 2015, the detection rate of leprosy in Santana do Ipanema municipality, Alagoas state, Brazil, was 39.3 cases per 100,000 inhabitants, and among young people below 15 years of age, it was 32.8 cases per 100,000 inhabitants. MATERIAL AND METHODS: A prospective study was carried out from 2015 to 2017, in Santana do Ipanema city, with 69 leprosy contacts in the age group of 4-15 years. Measurement of serum IgM, IgG, and IgA against phenolic glycolipid antigen-1 (PGL-1) was done by an indirect enzyme-linked immunosorbent assay. RESULTS: A high frequency of positive anti-PGL-1 IgM was found in both paucibacillary and multibacillary contacts. Twenty-three participants presented suspected lesions and 45 did not. In both groups a high frequency of positive IgM was found. In regard to anti-PGL-1 IgG, it was found a strong association between its positivity and the presence of lesions (relative risk of 3.25). Eight new cases of leprosy were diagnosed, five of which were seropositive for anti-PGL-1. Again, a striking association was found between positive IgG and leprosy (relative risk of 8.5). No significant association was found between IgM isotype and disease, nor between IgA and disease. CONCLUSIONS: The present study reinforces the importance of measuring the three anti-PGL-1 isotypes in follow-up studies of leprosy contacts. Moreover, positive anti-PGL-1 IgG is associated with a high associated risk of disease.

Polymorphism of IL10, IL4, CTLA4, and DAO Genes in Cross-Reactive Nonsteroidal Anti-inflammatory Drug Hypersensitivity.

Our aim was to evaluate genetic polymorphism of molecules involved in immunoregulatory/allergic processes in patients who presented with cutaneous hypersensitivity caused by chemically unrelated nonsteroidal anti-inflammatory drugs. Polymorphisms at IL10 (-1082 G>A), IL4 (-589 C>T), CTLA4 (+49A>G), and DAO (+8956 C>G) genes were studied in 55 cases and 97 controls by the polymerase chain reaction-restriction fragment length polymorphism technique. With regard to the polymorphism at IL10 -1082, higher frequencies of the AG genotype (57% vs 39%) and G allele carriers (70% vs 48%) were found among the patients, indicating a risk effect (odds ratio [OR] = 2.56 and P = .01 for AG genotype and OR = 2.52; P = .01 for AG/GG). For the CTLA4 +49 A/G single-nucleotide polymorphism (SNP), AG genotype (31.0%) (P = .02) and G carrier (54.0%) (P = .05) frequencies were found to be significantly lower in the patient group compared with the control group (51.0% and 69.0%, respectively). The SNP DAO +8956 C>G was associated with a strong protective effect, with OR values of 0.83 for CG and 0.11 for GG genotype (P = .04 for the codominant model), suggesting an allele dose effect. The combination of IL10 and DAO SNPs in a multivariate model did not alter the OR values, suggesting independent effects for both SNPs. The results are striking. In conclusion, these results suggest that polymorphisms in regulatory targets of the immune response and in DAO gene could modulate an individual's susceptibility to nonsteroidal anti-inflammatory drug hypersensitivity reactions. Further studies will be necessary to complement our results.

Serum anti-phenolic glycolipid-1 IgA correlates to IgM isotype in leprosy patients: a possible candidate for seroepidemiological surveys?

OBJECTIVE: The aim of this study was to compare serum anti-phenolic glycolipid-1 IgA, IgG, and IgM levels in leprosy patients and controls. METHOD: Analysis of anti-PGL-1 IgA, IgG, or IgM in serum samples from multibacillary (MB, n=32) and paucibacillary (PB, n=22) leprosy patients, and in non-endemic controls (n=17), using an indirect enzyme-linked immunosorbent assay. RESULTS: A strong correlation between serum IgM and IgA isotypes was found (r=.745, P<.0001) in MB patients. A moderate correlation was found in all analyses in PB patients. A moderate agreement was found between anti-PGL1 IgA and IgM tests. Based on the ROC curves, the cut-off values were selected and the parameters of validation were calculated. Considering the clinical forms altogether, the diagnostic sensitivities were 50.0% for IgA, 22.2% for IgG, and 74.1% for IgM. The positive (VPP) and negative (VPN) predictive values were estimated for each isotype. For IgA, the VPP and VPN were, respectively, 100.0% (87.0%-100.0%; 95% confidence interval) and 38.7% (24.4%-54.5%); for IgG, 100% (87.0%-100.0%) and 28.8% (17.8%-42.1%), respectively; and for IgM, 95.2% (83.8%-99.4%) and 51.7% (32.5%-70.6%), respectively. CONCLUSION: Despite the limiting factors, anti-PGL1 IgA correlates to IgM levels and it could be considered as a possible laboratorial tool to be also used, for instance, in serological follow-up studies.

CD38+CD8+ and CD38+CD4+ T Cells and IFN Gamma (+874) Polymorphism Are Associated with a Poor Virological Outcome.

The main objective of the work was to evaluate the use of CD38 on T lymphocytes, IFNγ (+874 A/T), and IL-10 (-1082 A/G) polymorphisms in HIV-infected patients under antiretroviral (ARV) therapy. Sixty-one patients were selected at the outpatient clinic for HIV infection at the Hospital São José de Doenças Infecciosas, Fortaleza, Ceará, Brazil. The patients were classified into two groups, according to viral load after one year of ARV therapy. In the aviremic group (group I), a reduction of 35.5% of CD38+CD4+ T cells was observed (p = 0.02) and 49.3% of CD38+CD8+ T cells (p = 0.001). In the viremic group (group II), a reduction of 37.2% of CD38+CD4+ T cells (p = 0.067), and 21.4% of CD38+CD8+ T cells (p = 0.60) occurred. No association was found between IL-10 (-1082) polymorphism and the type of response to ARV therapy. Regarding the gene polymorphism on IFNγ (+874 T/A), 73.34% of group I and 33.3% of group II presented the AA genotype. The relative risk of the individuals carrying AA genotype or the A allele and not being able to suppress the viral load level after one year of ARV therapy was 3.44 (1.25-9.45; p = 0.014) or 2.35 (1.05-5.26; p = 0.027), respectively. Our data suggested that an augmented frequency of activated CD38+CD8+ T cells as well as the presence of the A allele of IFNγ polymorphism could contribute to a reduced virological suppression in patients under antiretroviral therapy.

Assimetrias e similaridades na produção de cavalos no sul do Brasil e Argentina: produtividade, aspectos sanitários e comerciais / Asymmetries and similarities in horse production in the Southern of Brazil and Argentina: productivity, health and trade aspects

This study was carried out in order to verify the asymmetries and similarities in horse breeding in the Brazilian South region and Argentina, analyzing the productive, health and trade aspects. Data collection were performed with questionnaires in fieldwork (primary sources) and bibliographic searches and surveys (secondary sources). The sample consisted of horses breeders in Brazil, Rio Grande do Sul State, and in Argentina. The questionnaire was based on the Brazilian Study of Horse Agribusiness Complex, divided into: Production Aspects, Health Issues and trade aspects. Descriptive data analysis and relative frequencies were compared using the chi-square (χ2) test at 5% probability. It was observed that there was a similarity in several aspects related to the breeding and trade horse agribusiness, whereas in the health aspects there was asymmetry in certain aspects. In the Southern Brazil, 29.0% of the owners live in the breeding farm and 67.7% have another remunerated activity. There was a similarity in the endoparasites system control and an asymmetry in the ectoparasites control. There was a higher percentage of diseases control in Argentina, which may be related to technification and qualification of workforce. However, regarding the equine sanitary control, there was a similarity between the farms of Southern Brazil and Argentina. Despite of all asymmetries and similarities, there is a relevance of horse agribusiness on generating jobs and income source, as well as the cultural and economic contribution in both countries. Furthermore, there is a necessity of public policies from government authority to promote the horse industry.

O objetivo com este estudo foi observar as similaridades e asimetrias existentes na criação de cavalos no sul do Brasil e Argentina, a partir de análise dos aspectos produtivos, comerciais e da saúde dos equinos. A metodologia utilizada compreendeu levantamento de dados, através de aplicação de questionário a campo (fontes primárias) e pesquisas bibliográficas (fontes secundárias). A amostragem abrangeu criadores de equinos do Rio Grande do Sul e Argentina. O questionário foi elaborado baseado no Estudo do Complexo do Agronegócio Cavalo no Brasil, dividido em: Aspectos de Proodução, Sanitários e Comerciais. Foi realizada análise descritiva dos dados e as frequências relativas foram comparadas pelo teste Qui-quadrado (P<0,05). Observou-se similaridades nos aspectos relacionados à criação e comercialização, mas assimetria relacionada à saúde dos equinos. No sul do Brasil 29,0% dos proprietários vivem da criação de cavalos, enquanto 67,7% exercem outras atividades remuneradas. Observou-se similaridade no controle de endoparasitas e assimetria em relação à ectoparasitas. A alta percentagem de controle de doenças observada na Argentina pode estar relacionada à tecnificação e a mão de obra treinada. Contudo, observou-se similaridade entre as fazendas do sul do Brasil e Argentina em relação ao controle sanitário dos rebanhos de equinos. A respeito das diversas assimetrias e semelhanças observadas, destaca-se a importância do agronegócio cavalo na produção de emprego e renda, bem como a contribuição econômica e cultural para ambos os países. Além disso, há necessidade de políticas públicas para a promoção da indústria do cavalo.

Asymmetries and similarities in horse production in the Southern of Brazil and Argentina: productivity, health and trade aspects

AbstractThis study was carried out in order to verify the asymmetries and similarities in horse breeding in the Brazilian South region and Argentina, analyzing the productive, health and trade aspects. Data collection were performed with questionnaires in fieldwork (primary sources) and bibliographic searches and surveys (secondary sources). The sample consisted of horses breeders in Brazil, Rio Grande do Sul State, and in Argentina. The questionnaire was based on the Brazilian Study of Horse Agribusiness Complex, divided into: Production Aspects, Health Issues and trade aspects. Descriptive data analysis and relative frequencies were compared using the chi-square (2) test at 5% probability. It was observed that there was a similarity in several aspects related to the breeding and trade horse agribusiness, whereas in the health aspects there was asymmetry in certain aspects. In the Southern Brazil, 29.0% of the owners live in the breeding farm and 67.7% have another remunerated activity. There was a similarity in the endoparasites system control and an asymmetry in the ectoparasites control. There was a higher percentage of diseases control in Argentina, which may be related to technification and qualification of workforce. However, regarding the equine sanitary control, there was a similarity between the farms of Southern Brazil and Argentina. Despite of all asymmetries and similarities, there is a relevance of horse agribusiness on generating jobs and income source, as well as the cultural and economic contribution in both countries. Furthermore, there is a necessity of public policies from government authority to promote the horse industry.

ResumoO objetivo com este estudo foi observar as similaridades e asimetrias existentes na criação de cavalos no sul do Brasil e Argentina, a partir de análise dos aspectos produtivos, comerciais e da saúde dos equinos. A metodologia utilizada compreendeu levantamento de dados, através de aplicação de questionário a campo (fontes primárias) e pesquisas bibliográficas (fontes secundárias). A amostragem abrangeu criadores de equinos do Rio Grande do Sul e Argentina. O questionário foi elaborado baseado no Estudo do Complexo do Agronegócio Cavalo no Brasil, dividido em: Aspectos de Proodução, Sanitários e Comerciais. Foi realizada análise descritiva dos dados e as frequências relativas foram comparadas pelo teste Qui-quadrado (P 0,05). Observou-se similaridades nos aspectos relacionados à criação e comercialização, mas assimetria relacionada à saúde dos equinos. No sul do Brasil 29,0% dos proprietários vivem da criação de cavalos, enquanto 67,7% exercem outras atividades remuneradas. Observou-se similaridade no controle de endoparasitas e assimetria em relação à ectoparasitas. A alta percentagem de controle de doenças observada na Argentina pode estar relacionada à tecnificação e a mão de obra treinada. Contudo, observou-se similaridade entre as fazendas do sul do Brasil e Argentina em relação ao controle sanitário dos rebanhos de equinos. A respeito das diversas assimetrias e semelhanças observadas, destaca-se a importância do agronegócio cavalo na produção de emprego e renda, bem como a contribuição econômica e cultural para ambos os países. Além disso, há necessidade de políticas públicas para a promoção da indústria do cavalo.

Assimetrias e similaridades na produção de cavalos no sul do Brasil e Argentina: produtividade, aspectos sanitários e comerciais / Asymmetries and similarities in horse production in the Southern of Brazil and Argentina: productivity, health and trade aspects

This study was carried out in order to verify the asymmetries and similarities in horse breeding in the Brazilian South region and Argentina, analyzing the productive, health and trade aspects. Data collection were performed with questionnaires in fieldwork (primary sources) and bibliographic searches and surveys (secondary sources). The sample consisted of horses breeders in Brazil, Rio Grande do Sul State, and in Argentina. The questionnaire was based on the Brazilian Study of Horse Agribusiness Complex, divided into: Production Aspects, Health Issues and trade aspects. Descriptive data analysis and relative frequencies were compared using the chi-square (χ2) test at 5% probability. It was observed that there was a similarity in several aspects related to the breeding and trade horse agribusiness, whereas in the health aspects there was asymmetry in certain aspects. In the Southern Brazil, 29.0% of the owners live in the breeding farm and 67.7% have another remunerated activity. There was a similarity in the endoparasites system control and an asymmetry in the ectoparasites control. There was a higher percentage of diseases control in Argentina, which may be related to technification and qualification of workforce. However, regarding the equine sanitary control, there was a similarity between the farms of Southern Brazil and Argentina. Despite of all asymmetries and similarities, there is a relevance of horse agribusiness on generating jobs and income source, as well as the cultural and economic contribution in both countries. Furthermore, there is a necessity of public policies from government authority to promote the horse industry.(AU)

O objetivo com este estudo foi observar as similaridades e asimetrias existentes na criação de cavalos no sul do Brasil e Argentina, a partir de análise dos aspectos produtivos, comerciais e da saúde dos equinos. A metodologia utilizada compreendeu levantamento de dados, através de aplicação de questionário a campo (fontes primárias) e pesquisas bibliográficas (fontes secundárias). A amostragem abrangeu criadores de equinos do Rio Grande do Sul e Argentina. O questionário foi elaborado baseado no Estudo do Complexo do Agronegócio Cavalo no Brasil, dividido em: Aspectos de Proodução, Sanitários e Comerciais. Foi realizada análise descritiva dos dados e as frequências relativas foram comparadas pelo teste Qui-quadrado (P<0,05). Observou-se similaridades nos aspectos relacionados à criação e comercialização, mas assimetria relacionada à saúde dos equinos. No sul do Brasil 29,0% dos proprietários vivem da criação de cavalos, enquanto 67,7% exercem outras atividades remuneradas. Observou-se similaridade no controle de endoparasitas e assimetria em relação à ectoparasitas. A alta percentagem de controle de doenças observada na Argentina pode estar relacionada à tecnificação e a mão de obra treinada. Contudo, observou-se similaridade entre as fazendas do sul do Brasil e Argentina em relação ao controle sanitário dos rebanhos de equinos. A respeito das diversas assimetrias e semelhanças observadas, destaca-se a importância do agronegócio cavalo na produção de emprego e renda, bem como a contribuição econômica e cultural para ambos os países. Além disso, há necessidade de políticas públicas para a promoção da indústria do cavalo.(AU)

Asymmetries and similarities in horse production in the Southern of Brazil and Argentina: productivity, health and trade aspects

AbstractThis study was carried out in order to verify the asymmetries and similarities in horse breeding in the Brazilian South region and Argentina, analyzing the productive, health and trade aspects. Data collection were performed with questionnaires in fieldwork (primary sources) and bibliographic searches and surveys (secondary sources). The sample consisted of horses breeders in Brazil, Rio Grande do Sul State, and in Argentina. The questionnaire was based on the Brazilian Study of Horse Agribusiness Complex, divided into: Production Aspects, Health Issues and trade aspects. Descriptive data analysis and relative frequencies were compared using the chi-square (2) test at 5% probability. It was observed that there was a similarity in several aspects related to the breeding and trade horse agribusiness, whereas in the health aspects there was asymmetry in certain aspects. In the Southern Brazil, 29.0% of the owners live in the breeding farm and 67.7% have another remunerated activity. There was a similarity in the endoparasites system control and an asymmetry in the ectoparasites control. There was a higher percentage of diseases control in Argentina, which may be related to technification and qualification of workforce. However, regarding the equine sanitary control, there was a similarity between the farms of Southern Brazil and Argentina. Despite of all asymmetries and similarities, there is a relevance of horse agribusiness on generating jobs and income source, as well as the cultural and economic contribution in both countries. Furthermore, there is a necessity of public policies from government authority to promote the horse industry.

ResumoO objetivo com este estudo foi observar as similaridades e asimetrias existentes na criação de cavalos no sul do Brasil e Argentina, a partir de análise dos aspectos produtivos, comerciais e da saúde dos equinos. A metodologia utilizada compreendeu levantamento de dados, através de aplicação de questionário a campo (fontes primárias) e pesquisas bibliográficas (fontes secundárias). A amostragem abrangeu criadores de equinos do Rio Grande do Sul e Argentina. O questionário foi elaborado baseado no Estudo do Complexo do Agronegócio Cavalo no Brasil, dividido em: Aspectos de Proodução, Sanitários e Comerciais. Foi realizada análise descritiva dos dados e as frequências relativas foram comparadas pelo teste Qui-quadrado (P 0,05). Observou-se similaridades nos aspectos relacionados à criação e comercialização, mas assimetria relacionada à saúde dos equinos. No sul do Brasil 29,0% dos proprietários vivem da criação de cavalos, enquanto 67,7% exercem outras atividades remuneradas. Observou-se similaridade no controle de endoparasitas e assimetria em relação à ectoparasitas. A alta percentagem de controle de doenças observada na Argentina pode estar relacionada à tecnificação e a mão de obra treinada. Contudo, observou-se similaridade entre as fazendas do sul do Brasil e Argentina em relação ao controle sanitário dos rebanhos de equinos. A respeito das diversas assimetrias e semelhanças observadas, destaca-se a importância do agronegócio cavalo na produção de emprego e renda, bem como a contribuição econômica e cultural para ambos os países. Além disso, há necessidade de políticas públicas para a promoção da indústria do cavalo.

In vitro and in vivo leishmanicidal activity of Astronium fraxinifolium (Schott) and Plectranthus amboinicus (Lour.) Spreng against Leishmania (Viannia) braziliensis.

The aim of the present work was to evaluate antileishmanial activity of Astronium fraxinifolium and Plectranthus amboinicus. For the in vitro tests, essential oil of P. amboinicus (OEPA) and ethanolic extracts from A. fraxinifolium (EEAF) were incubated with 10(6) promastigotes of L. (Viannia) braziliensis. The OEPA was able to reduce the parasite growth after 48 h; nonetheless, all the EEAFs could totally abolish the parasite growth. For the in vivo studies, BALB/c mice were infected subcutaneously (s.c.) with 10(7) L. braziliensis promastigotes. Treatment was done by administering OEPA intralesionally (i.l.) for 14 days. No difference was found in lesion thickness when those animals were compared with the untreated animals. Further, golden hamsters were infected s.c. with 10(6) L. braziliensis promastigotes. The first protocol of treatment consisted of ethanolic leaf extract from A. fraxinifolium (ELEAF) administered i.l. for 4 days and a booster dose at the 7th day. The animals showed a significant reduction of lesion thickness in the 6th week, but it was not comparable to the animals treated with Glucantime. The second protocol consisted of 15 daily intralesional injections. The profiles of lesion thickness were similar to the standard treatment. In conclusion, in vivo studies showed a high efficacy when the infected animals were intralesionally treated with leaf ethanolic extract from A. fraxinifolium.

Interleukin-10 gene polymorphism (-1082G/A) and allergy to efavirenz in patients infected with human immunodeficiency virus.

The aim of the present study was to investigate the association between polymorphism in the interleukin-10 gene promoter at position -1082 in human immunodeficiency virus-infected patients who had presented allergic reaction due to efavirenz. The study included 63 patients treated at the Hospital São José de Doenças Infecciosas, Fortaleza, Ceará, Brazil. Twenty-one patients who had presented allergic reaction to efavirenz were compared to 42 patients with no allergic reaction following exposure to this drug. Blood samples were collected for DNA extraction and submitted to the restriction fragment length polymorphism - polymerase chain reaction technique. The -1082AA genotype was significantly more frequent in allergic patients as compared to non-allergic patients (p=0.019; χ(2)=5.534; OR=3.625; 95% CI=1.210-10.860). Likewise the allele IL-10 -1082A was identified significantly more often among efavirenz allergic patients than in the non-allergic group (p=0.009; χ(2)=6.787; OR=3.029; 95% CI=1.290-7.111). These findings suggest that the polymorphism in the interleukin-10 gene promoter -1082G/A can be related to the development of allergic reactions to efavirenz.