RESUMO
Copper nanoparticles-enzyme biohybrid is a promising material for the remediation of contaminated waters, but its function is influenced by its effect on degradation organic pollutants. This study is the first investigation into the fast degradation of a high amount of Bisphenol A (BPA) in water at neutral pH and room temperature. Four different CuNPs biohybrids with different cu species and nanoparticle sizes were used as catalysts. The biohybrid CuNPs@CALB-3, which contained Cu2O nanoparticles of around 10 nm size, showed excellent catalytic performance removing >95% BPA content (45 ppm) in an aqueous solution in 20 min in the presence of hydrogen peroxide at pH 8 using 1.5 g/L of a catalyst. The catalyst showed excellent stability and recyclability at these conditions.
RESUMO
This review describes the recent advances in the design of novel artificial metalloenzymes and their application in C-H activation reactions. The combination of enzymes and metal or organometallic complexes for the creation of new artificial metalloenzymes has represented a very exciting research line. In particular, the development of proteins with the ability to perform C-H functionalization presents a significant challenge. Here we discuss the development of these processes on natural metalloenzymes by using directed evolution, biotin-(strept)avidin technologies, photocatalytic hybrids or reconstitution of heme-protein technology.
