[Ultrastructural organization of nucleoli and ribosome level in lymphocytes at different stages of maturation in patients with chronic B-cell lymphocytic leukemia]. / Ul'trastrukturnaia organizatsiia iadryshek i uroven' ribosom v razlichnykh po stepeni zrelosti limfotsitakh krovi bol'nykh B-kletochnym khronicheskim limfoleikozom.

To clarify the role of the ribosome apparatus in the differentiation and maturation, the nucleolar ultrastructure, ribosome level and maturation stages of lymphocytes and their phorbol ester-induced differentiation capacity were studied immunologically, cytochemically and electron-microscopically in 16 patients with B-chronic lymphoid leukemia (B-CLL) and in one with small lymphocyte lymphoma. Most B-CLL cases had the clones mainly consisting of cells at an intermediate maturation stage. The cases with immature and mature lymphocyte clones were observed more rarely. The acid phosphatase activity did not correlate with the maturation stages of the lymphoid cells. The nucleolus activity ranged from virtually zero to high one. In general, there was a relationship between the ribosome content and nucleolus activity, although there were exceptions suggesting the impaired ribosome biogenesis. There was no correlation between the ribosome content and maturation degree of B-CLL lymphocytes and their phorbol-ester-induced differentiation capacity.

[Various morphofunctional characteristics of hematopoietic tissue in preleukemic conditions]. / Nekotorye morfofunktsional'nye osobennosti gemopoéticheskoi tkani pri predleikoznykh sostoianiiakh.

Cultural, ultrastructural, cytochemical and histological investigations of bone marrow tissue were conducted in 26 patients with varying types of preleukemia states. A number of early structural, morphological and functional signs of hemopoietic disorders of different character and manifestation degree have been revealed. Particularly, a relationship has been established between the pathologic changes appearing in the bone marrow stroma and disorders in the morphological and functional state of the hemopoietic cells.

[Dynamics of neutrophilic enzyme indicators in patients with peptic ulcer after autologous blood transfusion and hemosorption]. / Dinamika pokazatelei énzimaticheskogo statusa neitrofilov u bol'nykh iazvennoi bolezn'iu pod vliianiem autotransfuzii gemosorbentobrabotannoi krovi.

Autotransfusion of hemosorbent-treated blood was used as a method of stimulation of reparative regeneration processes in 52 patients with continuously non-healing gastric ulcers. In 35 patients (67%) the ulcers cicatrized during the first 2 weeks of the posttransfusion period. The investigation of the enzymatic status of neutrophils showed that autotransfusion of sorbent blood raised the functional activity of neutrophils and the cation protein level in them which could promote the cleansing of the ulcer surface from necrotic masses and intensify processes of cicatrization.

[Enzymatic markers in chronic myeloleukemia and their importance for identifying a blast crisis]. / Enzimaticheskie markery pri khronicheskom mieloleikoze i ikh znachenie dlia identifikatsii blastnogo kriza.

The paper summarizes data on the activity of adenosine deaminase and purine nucleoside phosphorylase which contribute to purine nucleotide degradation. The enzyme activity was studied in leukocytes of varying degree of differentiation obtained from 29 cases with chronic phase of chronic myeloid leukemia (CML), 19 patients with acute phase of the disease and from blasts of 32 cases with CML-associated blast crisis. In CML patients, lymphocytes of leukemic clones showed various levels of activity of the enzymes. Myeloid and lymphoid blast crises proved biochemically heterogeneous. The possibility to establish the nature of blast crisis in CML on the basis of profile of adenosine deaminase and purine nucleoside phosphorylase in blasts is discussed.

[Ultrastructure and cytochemical indices of lymphocytes from donors immunized with the rhesus-system antigen D(RhO)]. / Izuchenie ul'trastruktury i tsitokhimicheskikh pokazatelei limfotsitov donorov, immunizirovannykh antigenom D(RhO) sistemy rezus.

Distinctive metabolic and ultrastructural features have been found in peripheral blood lymphocytes of the donors underwent immunization with D(RhO) antigen. The degree of changes in the lymphocytes correlated well with the extent of immune responsiveness to the antigen in question.