Neonatal hyperoxia exposure leads to developmental programming of cardiovascular and renal disease in adult rats.

Premature infants are often exposed to hyperoxia. However, there is limited data regarding the mechanistic underpinnings linking neonatal hyperoxia exposure and its contribution to cardio-renal dysfunction in adults born preterm. Our objective was to determine whether neonatal hyperoxia induces systemic vascular stiffness and cardio-renal dysfunction in adulthood. Newborn rats were randomly assigned to room air (RA) or hyperoxia (85% O2) from postnatal day 1 to 14, then recovered in RA until 1 year of life. Arterial stiffness, cardio-renal histomorphometry, and fibrosis in the aorta, heart, and kidney were assessed. RNA-sequencing (RNA-seq) of the aorta and kidney was also done. Adult rats exposed to neonatal hyperoxia had increased aortic and mesenteric artery stiffness as demonstrated by wire and pressure myography. They also had cardiomyocyte hypertrophy, glomerulomegaly, and tubular injury. Hyperoxia exposure altered the transcriptome profile associated with fibrosis and matrix remodeling in the aorta and kidney. There was also increased TGF-ß1 levels and fibrosis in the aorta, left ventricle, and kidney. In conclusion, neonatal hyperoxia exposure was associated with systemic vascular and cardio-renal alterations in 1-year-old rats. Further studies to determine how targeted therapies could reprogram cardio-renal injury after neonatal hyperoxia exposure are indicated.

Programa de acompañamiento a personas diagnosticadas con cáncer de cabeza y cuello, liderado por la enfermera de práctica avanzada con mención en oncología / Support program for people with head and neck cancer, led by an oncology advanced practice nurse

Se presenta una propuesta de intervención de enfermería de práctica avanzada (EPA) en oncología. OBJETIVO: Implementar un programa de acompañamiento para pacientes con cáncer de cabeza y cuello. PROPUESTA: Realizar un levantamiento del proceso y descripción del flujo que debe transitar el paciente oncológico desde la sospecha, diagnóstico, tratamiento, seguimiento y derivación a cuidados paliativos. En segunda instancia, crear e implementar una consulta de enfermería que pueda responder a las necesidades de atención de salud en forma holística de la persona con diagnóstico oncológico de cáncer de cabeza y cuello, tanto en las personas que se encuentran en fase sospecha y confirmación diagnóstica (consulta de ingreso) y en el proceso de seguimiento del sobreviviente (después de finalizado el tratamiento oncológico), a través de una puesta en marcha escalonada, con períodos de evaluación en cada uno de ellos. CONCLUSIONES: La heterogeneidad de la persona diagnosticada con cáncer de cabeza y cuello representa un desafío y se considera a la EPA un profesional para manejar casos complejos. El camino hacia este nuevo rol será paulatino, y requiere una transición tanto por el equipo de salud, los pacientes y las propias enfermeras dentro del marco legal vigente.

This paper presents a proposal for an advanced practice nursing intervention (APN) in oncology. OBJECTIVE: To implement a support program for patients with head and neck cancer. PROPOSAL: To carry out a survey of the process and description of the flow that the oncology patient must go through from suspicion, diagnosis, treatment, follow-up and referral to palliative care. Secondly, to create and implement a nursing consultation that can respond to the health care needs in a holistic manner of the person with an oncological diagnosis of head and neck cancer, both in people who are in the suspicion and diagnostic confirmation phase (admission consultation) and in the follow-up process of the survivor (after the end of oncological treatment), through a staggered implementation, with evaluation periods in each of them. CONCLUSIONS: The heterogeneity of the person diagnosed with head and neck cancer represents a challenge and the APN is considered a professional to manage complex cases. The journey towards this new role will be gradual, and requires a transition both by the health team, the patients and the nurses themselves within the current legal framework.

Relación del apoyo social percibido y el logro de las metas de compensación de los pacientes con hipertensión arterial y diabetes mellitus / Relationship of perceived social support and the compensation goals of patients with arterial hypertension and diabetes mellitus

INTRODUCCIÓN: Las enfermedades crónicas son un problema actual que presentan altos porcentajes de descompensación en pacientes hipertensos y diabéticos, que hace interesante evaluar los determinantes sociales que influyen en la situación, como el apoyo social. OBJETIVO DEL ESTUDIO: fue relacionar el apoyo social percibido, adherencia a medicamentos, literacidad y variables sociodemográficas con el logro de compensación en pacientes con hipertensión arterial y diabetes mellitus. METODOLOGÍA: De tipo descriptivo correlacional de corte transversal. RESULTADOS: Se estudió a 600 personas, con un promedio de edad de 62,9 años, 63,5% mujeres. Un 64,66% son diabéticos y 89,5% hipertensos (46,9% y 43,33% descompensados respectivamente). El apoyo social percibido fue alto y presentan una inadecuada adherencia al tratamiento farmacológico y una adecuada literacidad en salud (57,6%). Se realiza un análisis de relaciones que demuestran relación significativa entre una menor percepción de apoyo instrumental y ser mujer; la percepción del apoyo de interacción social disminuye con el aumento de la edad y en relación con el apoyo afectivo, disminuye la percepción a mayores años de estudios. Al realizar una regresión logística, entre la percepción de apoyo social baja y los niveles de compensación de los parámetros de compensación no se evidencia una relación estadísticamente significativa. Se evidencia una asociación entre las personas con menos de 8 años de estudio y presentar presiones arteriales elevadas. DISCUSIÓN: Existe dificultad en la medición del concepto de apoyo social, dado que el sentido y significancia es personal. Las patologías crónicas como estresores debe ser un elemento que continúe estudiándose con estudios longitudinales y experimentales. Las estrategias clínicas para la compensación de la hipertensión y la diabetes deben ir más allá del apoyo social, considerando las crecientes complicaciones y proyecciones de mortalidad. CONCLUSIÓN: Se destaca la importancia de abordar el apoyo social considerando su naturaleza subjetiva y contextual, aunque no se estableció una correlación directa con el logro terapéutico. Se señala la inadecuada adherencia al tratamiento farmacológico, la asociación entre baja escolaridad y presiones arteriales elevadas.

INTRODUCTION: Chronic diseases are a current problem since there are high percentages of decompensation in hypertensive and diabetic patients, which makes it interesting to evaluate the social determinants that influence and social support as a multidimensional concept. OBJECTIVE: To relate the perceived social support, medication adherence, literacy and sociodemographic variables with the achievement of compensation in patients with arterial hypertension and / or diabetes mellitus. METHODOLOGY: Descriptive correlational descriptive study is performed methodologically. RESULTS: 600 people were studied, with an average age of 62.9 years, 63.5% women. 64.66% are diabetic and 89.5% hypertensive (46.9% and 43.33% decompensated respectively). The perceived social support was high and showed inadequate adherence to drug treatment and adequate literacy (57.6%). An analysis of relationships that are significantly related between a lower perception of instrumental support and being a woman is performed; the perception of social interaction support with increasing age and in relation to affective support, the perception of older years of studies. When performing a logistic regression, there is no statistically significant relationship between the perception of low social support and the compensation levels of the compensation parameters. There is evidence of an association between children under 8 years of study and presentation of high arterial references. DISCUSSION: There is difficulty in measuring the concept of social support, since the meaning and meaning is personal. Chronic pathologies as stressors should be an element that continues to study with longitudinal and experimental studies. Clinical strategies for the compensation of hypertension and diabetes must go beyond social support, considering the increasing complications and mortality projections. CONCLUSION: Emphasizes the importance of addressing social support considering its subjective and contextual nature, though no direct correlation with therapeutic achievement was established. Highlights inadequate adherence to pharmacological treatment, the association between low education and elevated blood pressure.

Percepciones de las enfermeras de oncología infantil relacionadas con el perfil, capacitación, emociones y reacciones en la valoración del niño con dolor / Perceptions of pediatric oncology nurses related to the profile, training, emotions, and reactions in the assessment of children with pain

El cáncer es una de las principales causas de mortalidad en la niñez y la adolescencia. Tanto el impacto biopsicosocial y espiritual que produce, puede llevar al niño a experimentar dolor y sufrimiento. OBJETIVO: Comprender aspectos comunes percibidos por la enfermera oncológica y como se relacionan en la valoración de un niño con dolor. MÉTODO: se trató de un estudio cualitativo con diseño de teoría fundamentada de datos según referencial metodológico de Strauss y Corbin. Por medio de muestreo teórico fueron seleccionados 13 participantes profesionales de enfermería pertenecientes a diferentes hospitales de Santiago de Chile. Los datos fueron recolectados a través de entrevistas semiestructuradas y en profundidad. El estudio se rigió por medio de los criterios de credibilidad, confirmabilidad y fidelidad de Guba y Lincoln. Los datos recolectados fueron transcritos posterior a cada entrevista y analizados en tres etapas: codificación abierta, axial y selectiva. RESULTADOS: para las enfermeras que trabajan en el área de oncología infantil, es central el cuidado entregado en el proceso de enfermedad del niño hospitalizado por cáncer, considerando aspectos como el perfil de las profesionales, las emociones, la capacitación, las reacciones frente a un niño con dolor y las relaciones que existe entre ellos. CONCLUSIONES: Existen aspectos que resultan común en las enfermeras que se desempeñan en oncología infantil y que producto de las relaciones existentes entre ellos, pueden inclinar a las profesionales a reconocer el dolor en los niños con cáncer desde diversas perspectivas.

Cancer is one of the main causes of mortality in childhood and adolescence. Both the biopsychosocial and spiritual impact it produces can lead the patient child to experience pain and suffering. OBJETIVE: To understand common aspects perceived by the oncology nurse and how they are related to the assessment of a child in pain. METHOD: We conducted a qualitative study with a grounded theory approach based on Strauss and Corbin's methodological framework. Through theoretical sampling, 13 nursing professionals who worked in different hospitals in Santiago, Chile were selected. Data collection took place through semi-structured, in-depth interviews. The study followed Guba and Lincoln's criteria for credibility, confirmability, and fidelity. The data collected was transcribed after each interview and analyzed in three stages: open, axial and selective coding. RESULTS: For nurses who work in pediatric oncology, the care provided throughout the illness process for the child is central, considering aspects such as the profile of the professionals, the emotions, training, reactions to a child in pain, and the relationship that exist amongst them. CONCLUSIONS: There are common aspects amongst nurses who work in pediatric oncology. Because of the relationships between them, these aspects can help professionals to recognize pain in children with cancer following diverse perspectives.

Enfermedad crónica y control metabólico, relación con características de la atención de salud en Chile / Chronic disease and metabolic control, relationship with characteristics of health care in Chile

OBJETIVO: determinar el nivel de control metabólico de las personas con hipertensión arterial (HTA) y diabetes mellitus (DM) que se atienden en el Programa de Salud Cardiovascular en la red de Atención Primaria en Salud (APS) a nivel nacional y establecer su relación con algunas características de la atención de salud. MÉTODO: diseño descriptivo, correlacional y de corte transversal. Se realizó estadística descriptiva del control metabólico de las personas con HTA y DM, se utiliza Prueba T para Muestras pareadas. Se calcula la asociación entre el control metabólico y la variable de población beneficiaria por comuna utilizando el Coeficiente de Correlación de Pearson. RESULTADOS: en el control metabólico, de las personas con HTA, el 56,02% y 55,75% logran una presión arterial <140/90 mmHg en el año 2018 y 2019; con respecto a las personas con DM, el 38,73% y el 38,51% alcanzan niveles de hemoglobina glicosilada <7% para los años 2018 y 2019 respectivamente. Se evidencia una correlación positiva entre el control metabólico de HTA y DM, con un R de Pearson = 0,719, un IC 95% [0,453 ­ 0,868] y p<0,001 en el año 2018 y un R de Pearson = 0,647, un IC 95% [0,330 ­ 0,833] y p<0,001 en el año 2019. CONCLUSIONES: adoptar un enfoque integral y de género en el manejo de las enfermedades crónicas en la Atención Primaria en Salud es fundamental, dirigiendo las estrategias con el fin de lograr el control metabólico. Las políticas públicas deben orientarse a la disminución de las brechas en salud.

OBJECTIVE: to determine the level of metabolic control of people with high blood pressure (HBP) and diabetes mellitus (DM) who are treated in the Cardiovascular Health Program within the primary care network at the national level and to establish their relationship with some characteristics of health care provision. METHOD: cross-sectional correlational descriptive design. Descriptive statistics of the metabolic control of people with HBP and DM were performed, using the T Test for Paired Samples. The association between metabolic control and the beneficiary population variable by commune is calculated using the Pearson Correlation Coefficient. RESULTS: in relation to the metabolic control, 56.02% and 55.75% of people with hypertension, in 2018 and 2019, achieved a blood pressure <140/90 mmHg; regarding people with DM, 38.73% and 38.51%, obtained glycosylated hemoglobin levels <7%, for the year 2018 and 2019. A positive correlation is evidenced between the metabolic control of HBP and DM, with a Pearson's R = 0.719, a 95% CI [0.453 - 0.868] and p<0.001 in the year 2018 and a Pearson's R = 0.647, a 95% CI [0.330 - 0.833] and p<0.001 in the year 2019. CONCLUSIONS: adopting a comprehensive and gender approach in the management of chronic diseases in Primary Health Care is essential, directing strategies to achieve metabolic control. Public policies must be aimed at reducing health gaps.

Nivel de literacidad en salud en población chilena con enfermedades crónicas: análisis secundario de la Encuesta Nacional de Salud 2009 y 2016 / Health literacy level on chilean population with chronic diseases: secondary analysis of the 2009 and 2016 national health survey

INTRODUCCIÓN: Las enfermedades crónicas no transmisibles (ECNT) son consideradas una pandemia dada su alta prevalencia y morbimortalidad asociada. Se postula que el nivel de literacidad en salud (LS) de las personas con estas condiciones impactaría en su autocuidado. OBJETIVO: Conocer la prevalencia de bajo nivel de LS de la población chilena con ECNT según datos de la Encuesta Nacional de Salud (ENS) versión 2009 y 2016; además de identificar cómo su LS influiría sobre ciertos resultados en salud. METODOLOGÍA: Estudio transversal descriptivo-analítico. Se utiliza el ítem del módulo psicosocial de la ENS que evalúa autoeficacia literaria y se cruza con variables sociodemográficas y resultados intermedios de salud de interés. RESULTADOS Y DISCUSIÓN: La prevalencia de bajo nivel de LS en población con diabetes fue de 23,7% el 2009 y de 15,5%, el 2016. Para personas con hipertensión arterial, se obtuvo una prevalencia de 19,7% en el 2009 y de 17,5% en el 2016. Se observa diferencias por edad, sexo, nivel educacional y ruralidad. Se reporta mayor proporción de HbA1c descompensada, menor actividad física y peor estado de salud percibido en la población estudiada. CONCLUSIONES: El nivel de LS influye en cómo los individuos manejan su condición crónica. Contar con un diagnóstico previo del nivel de LS de las personas, permitiría diseñar intervenciones pertinentes a sus necesidades.

INTRODUCTION: Chronic non-communicable diseases (NCDs) are considered a pandemic given their high prevalence and associated morbidity and mortality. It is postulated that the level of health literacy (HL) of people with these conditions would impact their self-care. OBJECTIVE: To know the prevalence of low HL of the Chilean population with NCD according to data from the National Health Survey (ENS) version 2009 and 2016; in addition to identify how their HL would influence certain health outcomes. METHODOLOGY: Descriptive-analytical cross-sectional study. The ENS's item from the psychosocial module which evaluates literary self-efficacy is used and crossed with sociodemographic variables and intermediate health outcomes of interest. RESULTS AND DISCUSSION: The prevalence of low HL in the population with diabetes was 23.7% in 2009 and 15.5% in 2016. For people with arterial hypertension, a prevalence of 19.7% was obtained in the 2009 and 17.5% in 2016. Differences are observed by age, sex, educational level, and rurality. A higher proportion of uncompensated HbA1c, lower physical activity and worse perceived health status are reported in the population studied. CONCLUSIONS: The HL level influences how individuals manage their chronic condition. Having a prior diagnosis of people's HL would allow the design of interventions to be relevant to their needs.

Educación en enfermería y cuidado de personas con condiciones crónicas: avances y desafíos / Nursing education and care for people with chronic conditions: advances and challenges

INTRODUCCIÓN: El escenario mundial actual de las enfermedades crónicas (EC) es preocupante. Representan el grueso de la carga de enfermedad, en una población envejecida y con alta prevalencia de factores de riesgo. Para mejorar los resultados de las personas con EC, una de las líneas de trabajo prioritarias es la incorporación del apoyo al automanejo, como parte del rol profesional de profesionales de la salud. La principal debilidad percibida hoy es la falta de formación teórico-práctica desde la formación de pregrado. OBJETIVO: evaluar la enseñanza acerca del cuidado en condiciones crónicas de salud, en la formación de pregrado de la Escuela de Enfermería. METODOLOGÍA: Estudio mixto que incluyó a) Análisis del plan de estudios de pregrado vigente 2) Exploración de la experiencia previa de la unidad en investigación y educación continua en cuidados crónicos; consulta con expertos, incluyendo miembros clínicos de equipos de salud que participan en la atención a crónicos. 3) Revisión de la literatura de modelos teóricos para los cuidados crónicos 4) Explorar, a través de las percepciones de los profesores, el grado de implementación de los cuidados de enfermería para las EC. RESULTADOS: Se detectó un bajo nivel de incorporación de la educación para el cuidado crónico en el currículum y ausencia de uso de conceptos claves relacionados con esto. Esto debe hacerse a lo largo de un proceso educativo que progresivamente aumenta el nivel de complejidad en la aplicación de los cuidados a las personas con EC y que se encuentra en implementación en un nuevo plan de estudio en ejecución desde el año 2022.

INTRODUCTION: The global current scenario in non-communicable diseases (NCD) is worrisome. In Chile these conditions represent the bulk of the disease burden, in an ageing population with a high prevalence of risk factors for NCDs. Thus, the training of health professionals in this field is paramount. PURPOSE: to share our experience at the school of nursing (SON) in designing and integrating chronic care and self-management support for people with chronic conditions in the undergraduate program. METHODOLOGY: a) Revision of the undergraduate curriculum 2) Exploring SON previous experience in research and continuing education in chronic care; consultation with experts including clinical members of health teams that participate in delivering chronic care. 3) Review of the literature to find theoretical models for chronic care 4) explore, through the lecturers' perceptions, the degree of implementation of nursing care for NCDs in clinical practice. RESULTS AND CONCLUSIONS: We found that there was a lack of incorporation of subjects and contents on chronic care in the curriculum and in clinical settings. It is important to incorporate chronic care and self-management support in nursing education at the theoretical and the clinical level. This should be done throughout an education process that increases progressively the level of complexity in the application of caring for people with NCDs. The SON will implement this approach in the new curricula starting on 2022.

Uso de inhaladores para la enfermedad pulmonar obstructiva crónica en un grupo de pacientes colombianos

Introducción: En Colombia son escasos los datos sobre el uso de los inhaladores en pacientes con EPOC. Objetivo: Describir la técnica de uso de inhaladores de dosis medida y polvo seco en pacientes de un hospital colombiano. Materiales y métodos: Estudio descriptivo en pacientes mayores de 40 años con EPOC atendidos en un hospital en La Virginia, Risaralda, Colombia, entre el 1 de septiembre de 2019 al 31 de enero de 2020. La unidad de análisis fueron los pacientes. Se incluyeron variables sociodemográficas, clínicas y lista de chequeo para uso de inhaladores. Se aplicaron frecuencias y proporciones para variables discretas, estadísticas de tendencia central y dispersión para variables continuas. Resultados: Se incluyeron 104 pacientes con edad media de 73,6 ± 10,1 años; 57 eran mujeres (54,8 %). Además, 48 pacientes estaban clasificados como GOLD-D (46,2 %). Igualmente, 89 pacientes manifestaron haber recibido educación sobre el uso de broncodilatadores (85,6 %). Los más frecuentes fueron los inhaladores de dosis medida (DM) en 95 casos (91,3 %), seguido de los de polvo seco unidosis (7,7 %). Así mismo, 37 pacientes que usaron DM sin inhalocámara (35,6 %) no cumplieron los pasos de la lista de chequeo. En el sistema multidosis, el más realizado fue cerrar de manera adecuada el inhalador y el menos ejecutado, expulsar el aire lentamente evitando hacerlo cerca del inhalador (n = 6; 5,7 %). Discusión: Se lograron describir las características de la técnica de uso de los inhaladores en pacientes con EPOC. A pesar de que ningún paciente logró utilizar el inhalador de forma "perfecta", la mayoría han recibido educación por parte de los profesionales de la salud. Conclusión: Un alto porcentaje de pacientes usa inadecuadamente los dispositivos para suministrar los broncodilatadores. Esto puede impactar negativamente en el control de la enfermedad.

Introduction: In Colombia, there is limited data on the use of inhalers in patients with COPD. Objective: The objective was to describe the technique of using metered-dose inhalers and dry powder in patients in a Colombian hospital. Methods: Observational, descriptive study of patients over 40 years of age with COPD, treated in a hospital in La Virginia, Risaralda, Colombia, between September 1st, 2019 and January 31st, 2020. The unit of analysis were patients in consultation. Sociodemographic and clinical variables, and a checklist for use of inhalers were included. Frequencies and proportions were applied for discrete variables, statistics of central tendency and dispersion for continuous variables. Results: A total of 104 patients with an average age of 73.6 ± 10.1 years were included; 57 were women (54.8%). In addition, 48 patients were classified as GOLD-D (46.2%). Similarly, 89 patients reported having received education on the use of bronchodilators (85.6%). The most common were metered-dose (MD) inhalers in 95 cases (91.3%), followed by single-dose dry powder inhalers in eight patients (7.7%). Likewise, 37 patients who used DM without inhalochamber (35.6%) did not comply with the steps of the checklist. In the multidose system, the most performed was to properly close the inhaler and the least performed was to expel the air slowly, avoiding doing so near the inhaler (n=6; 5.7%). Discussion: The characteristics of the technique of using inhalers in patients with COPD were described. Although no patient was able to use the inhaler "perfectly", most have received education from health professionals. Conclusion: A high percentage of patients misuse the devices to deliver bronchodilators. This can negatively impact the control of the disease.

Biochemical, Catabolic, and PGP Activity of Microbial Communities and Bacterial Strains from the Root Zone of Baccharis linearis in a Mediterranean Mine Tailing.

The management of mine tailings (MT) is commonly workload heavy, intrusive, and expensive. Phytostabilization offers a promising approach for MT management; however, it poses challenges due to the unfavorable physicochemical properties of these wastes. Nevertheless, native microorganisms capable of supporting plant growth and development could enhance the efficacy of phytostabilization. This study assesses the biological activity of microbial communities from the root zone of Baccharis linearis, which is naturally present in MT, in order to evaluate their biotechnological potential for phytostabilization. The root zone and bulk samples were collected from B. linearis plants located within a MT in the Mediterranean zone of Chile. Enzyme activities related to the cycling of C, N, and P were assessed. The community-level physiological profile was evaluated using the MicroRespTM system. Bacterial plant growth-promoting (PGP) traits and colony forming units (CFU) were evaluated through qualitative and microbiological methods, respectively. CFU, enzyme activities, and CLPP were higher in the root zone compared with the bulk samples. Five bacterial strains from the root zone exhibited PGP traits such as P solubilization and N acquisition, among others. The presence of microbial communities in the root zone of B. linearis with PGP traits suggests their potential to enhance the ecological management of MT through phytostabilization programs.

El sentimiento de soledad en la población adulta mayor participante en centros de día en Bogotá, Colombia

Objetivo: Identificar los factores asociados a las categorías de soledad en población adulta mayor en centros de día en Bogotá, Colombia. Materiales y métodos: Se realizó un estudio cuantitativo, transversal y analítico para medir la soledad en personas mayores que participan en un centro de día de la ciudad de Bogotá, mediante la escala ESTE en personas mayores, entre noviembre de 2020 y junio de 2021. Para cumplir con el objetivo, se realizó un análisis estadístico descriptivo univariado, de tal forma que, para las variables cuantitativas, se empleó media con desviación estándar o mediana con rangos intercuartílicos, de acuerdo con los criterios de normalidad utilizando la prueba Shapiro Wilk, y para las variables categóricas, frecuencias absolutas y proporciones. El análisis bivariado se realizó con las pruebas t de Student y chi cuadrado (p < 0,05), lo cual contribuyó a la construcción de un modelo de regresión logística, con las variables con significancia estadística. Resultados: Se incluyeron 215 personas adultas mayores con promedio de edad de 70,5 años; 72 % correspondían al sexo femenino, 56,5 % tenían educación primaria, 38,6 % eran solteros y 67,4 % presentaban antecedente de enfermedad crónica no transmisible. Según la escala ESTE, en soledad familiar registraron nivel bajo (67 %); en soledad conyugal, entre nivel alto y medio (79 %); en soledad social, nivel alto y medio (51 %); en crisis de adaptación, nivel alto y medio (43 %). Se encontraron asociaciones en soledad conyugal con sexo femenino (p = 0,001), en soledad social con clase baja (p = 0,027) y en crisis de adaptación con clase baja (p = 0,024). Conclusiones: Los factores que se asocian al sentimiento de soledad en población adulta mayor participante en centros de día son, en la categoría de soledad conyugal, ser mujer, y, en soledad social y crisis de adaptación, pertenecer a clase baja.

Objective: To identify the factors associated with the categories of loneliness among the elderly population attending day care centers in Bogotá, Colombia. Materials and methods: An analytical, cross-sectional and quantitative study was carried out to measure the loneliness among older people attending a day care center in the city of Bogotá between November 2020 and June 2021 using the ESTE scale. To meet the objective, a univariate descriptive statistical analysis was performed, such that, for the quantitative variables, the mean with standard deviation or median with interquartile ranges were used, in accordance with the Shapiro-Wilk test for normality, and for the categorical variables, absolute frequencies and proportions were used. The bivariate analysis was conducted using Student's t-test and chi-square test (p < 0.05), which contributed to build a logistic regression model with statistically significant variables. Results: A total of 215 elderly people with a mean age of 70.5 years were included in the study: 72 % were females, 56.5 % had primary education, 38.6 % were single and 67.4 % had a history of chronic non-communicable disease. According to the ESTE scale, the study subjects showed a low level of family loneliness (67 %), a high and medium level of marital loneliness (79 %), a high and medium level of social loneliness (51 %) and a high and medium level of adaptation crisis (43 %). It was found that marital loneliness was associated with females (p = 0.001), social loneliness with lower class (p = 0.027) and adaptation crisis with lower class (p = 0.024). Conclusions: The factors associated with the feeling of loneliness among the elderly population attending day care centers are, in the marital loneliness category, being a woman and, in the social loneliness and adaptation crisis categories, belonging to the lower class.

Implementación de tres guías de buenas prácticas clínicas en la escuela de enfermería de la universidad de talca, chile

El presente artículo describe el proceso de implementación de tres guías elaboradas por la Registered Nurses' Association of Ontario (RNAO) de Canadá, en el módulo enfermería en el adulto en la Escuela de Enfermería de la Universidad de Talca, proceso que se enmarca en el convenio establecido entre la Universidad de Talca y esta asociación, a través del Departamento de Enfermería de la Universidad de Chile, host leader del Programa de Guías de Buenas Prácticas Clínicas de la RNAO. Esta implementación se llevó a cabo a través de diversas metodologías de enseñanza-aprendizaje que han permitido a los estudiantes integrar y aplicar la Enfermería Basada en Evidencia en la práctica clínica y también, establecer una alianza entre la institución académica y los centros de asistencia sanitaria.

This article describes the implementation process of three guidelines developed by the Registered Nurses' Association of Ontario (RNAO) in the adult nursing module at the School of Nursing of the University of Talca. This process is part of the agreement reached between the University of Talca and this association, through the Department of Nursing of the University of Chile, Host Leader of the RNAO's Best Clinical Practice Guidelines Program. This implementation has been carried out through various teaching-learning methodologies that have allowed students to integrate and apply Evidence-Based Nursing in clinical practice and also to establish an alliance between the academic institution and health care centers.

Este artigo descreve o processo de implementação de três diretrizes desenvolvidas pela Associação de Enfermeiros Registrados de Ontario (RNAO, sigla em inglês) do Canadá no módulo de enfermagem para adultos da Escola de Enfermagem da Universidade de Talca. Esse processo faz parte do acordo estabelecido entre a Universidade de Talca e esta associação, por meio do Departamento de Enfermagem da Universidade do Chile, host leader do Programa de Boas Práticas Clínicas da RNAO. Essa implementação foi realizada por meio de várias metodologias de ensino-apredizagem que permitiram aos estudantes integrarem a Enfermagem Baseada em Evidências à prática clínica, e também estabelecer uma aliança entre a instituição académica e os centros de saúde.

Winogradsky Bioelectrochemical System as a Novel Strategy to Enrich Electrochemically Active Microorganisms from Arsenic-Rich Sediments.

Bioelectrochemical systems (BESs) have been extensively studied for treatment and remediation. However, BESs have the potential to be used for the enrichment of microorganisms that could replace their natural electron donor or acceptor for an electrode. In this study, Winogradsky BES columns with As-rich sediments extracted from an Andean watershed were used as a strategy to enrich lithotrophic electrochemically active microorganisms (EAMs) on electrodes (i.e., cathodes). After 15 months, Winogradsky BESs registered power densities up to 650 µWcm-2. Scanning electron microscopy and linear sweep voltammetry confirmed microbial growth and electrochemical activity on cathodes. Pyrosequencing evidenced differences in bacterial composition between sediments from the field and cathodic biofilms. Six EAMs from genera Herbaspirillum, Ancylobacter, Rhodococcus, Methylobacterium, Sphingomonas, and Pseudomonas were isolated from cathodes using a lithoautotrophic As oxidizers culture medium. These results suggest that the tested Winogradsky BES columns result in an enrichment of electrochemically active As-oxidizing microorganisms. A bioelectrochemical boost of centenarian enrichment approaches, such as the Winogradsky column, represents a promising strategy for prospecting new EAMs linked with the biogeochemical cycles of different metals and metalloids.

Microbial Community-Level Physiological Profiles and Genetic Prokaryotic Structure of Burned Soils Under Mediterranean Sclerophyll Forests in Central Chile.

Forest fires alter soil microbial communities that are essential to support ecosystem recovery following land burning. These alterations have different responses according to soil abiotic pre- and post-fire conditions and fire severity, among others, and tend to decrease along vegetation recovery over time. Thus, understanding the effects of fires on microbial soil communities is critical to evaluate ecosystem resilience and restoration strategies in fire-prone ecosystems. We studied the state of community-level physiological profiles (CLPPs) and the prokaryotic community structure of rhizosphere and bulk soils from two fire-affected sclerophyll forests (one surveyed 17 months and the other 33 months after fire occurrence) in the Mediterranean climate zone of central Chile. Increases in catabolic activity (by average well color development of CLPPs), especially in the rhizosphere as compared with the bulk soil, were observed in the most recently affected site only. Legacy of land burning was still clearly shaping soil prokaryote community structure, as shown by quantitative PCR (qPCR) and Illumina MiSeq sequencing of the V4 region of the 16S rRNA gene, particularly in the most recent fire-affected site. The qPCR copy numbers and alpha diversity indexes (Shannon and Pielou's evenness) of sequencing data decreased in burned soils at both locations. Beta diversity analyses showed dissimilarity of prokaryote communities at both study sites according to fire occurrence, and NO3 - was the common variable explaining community changes for both of them. Acidobacteria and Rokubacteria phyla significantly decreased in burned soils at both locations, while Firmicutes and Actinobacteria increased. These findings provide a better understanding of the resilience of soil prokaryote communities and their physiological conditions in Mediterranean forests of central Chile following different time periods after fire, conditions that likely influence the ecological processes taking place during recovery of fire-affected ecosystems.

Cuidado de transición: calidad y seguridad de la atención en personas con condiciones crónicas de salud / Transitional care for people with chronic health conditions

People with chronic conditions and elderly frequently use different levels of health care. If those are not coordinated, patients are exposed to complications and adverse effects. To avoid this, the implementation of transitional care (TC) is proposed, which includes the coordination and follow-up of users through the different care settings. We aimed to analyze the concept of transitional care, its characteristics and impact on people with chronic conditions. A search of articles published between 2012 and 2019 in Google Scholar, MEDLINE, CINAHL and Cochrane Library databases was conducted looking for articles related to the concept of transitional care, characteristics, and impact on people with chronic conditions. Several models of TC have been formulated and the literature identifies common interventions, namely telephone follow-up, outpatient clinics and home visits for self-management support. Reports show that TC reduces re-hospitalizations, care costs, increases quality of life and user satisfaction with the health system.

[Transitional care for people with chronic health conditions]. / Cuidado de transición: calidad y seguridad de la atención en personas con condiciones crónicas de salud.

People with chronic conditions and elderly frequently use different levels of health care. If those are not coordinated, patients are exposed to complications and adverse effects. To avoid this, the implementation of transitional care (TC) is proposed, which includes the coordination and follow-up of users through the different care settings. We aimed to analyze the concept of transitional care, its characteristics and impact on people with chronic conditions. A search of articles published between 2012 and 2019 in Google Scholar, MEDLINE, CINAHL and Cochrane Library databases was conducted looking for articles related to the concept of transitional care, characteristics, and impact on people with chronic conditions. Several models of TC have been formulated and the literature identifies common interventions, namely telephone follow-up, outpatient clinics and home visits for self-management support. Reports show that TC reduces re-hospitalizations, care costs, increases quality of life and user satisfaction with the health system.

[Advanced practice nursing as a proposal to improve access and coverage in oncology for Latin America]. / Una propuesta para mejorar el acceso y cobertura en oncología para Latinoamérica: enfermería de práctica avanzada.

PAHO/WHO proposes to implement the role of Advanced Practice Nurse (APN) in Latin America, to reduce gaps in coverage and access to health care. For this purpose, it is necessary to train nursing professionals with an expanded role, which allows them to collaborate in the diagnosis, treatment, and monitoring of people with specific diseases, under established protocols and clinical guidelines and within consolidated interdisciplinary health teams in a cost-effective way. One of the areas with the greatest coverage deficit in Latin America is adult oncology, with inequality in care opportunities for these patients. Part of the premature deaths attributable to this disease are due to the lack of access to timely diagnosis and treatment. As a contribution to the reduction of this gap, a training program of Advanced Nursing Practice was developed, addressing the most urgent needs in the field of oncology. The necessary competencies were defined to develop a professional-level master's degree program, considering prevention, early detection, diagnosis, treatment, and monitoring of people with oncological diseases at different levels of care. A program of this kind is an opportunity to reduce the access gap and coverage of health care for people with cancer, improving their quality of life and their survival.

Una propuesta para mejorar el acceso y cobertura en oncología para Latinoamérica: enfermería de práctica avanzada / Advanced practice nursing as a proposal to improve access and coverage in oncology for Latin America

PAHO/WHO proposes to implement the role of Advanced Practice Nurse (APN) in Latin America, to reduce gaps in coverage and access to health care. For this purpose, it is necessary to train nursing professionals with an expanded role, which allows them to collaborate in the diagnosis, treatment, and monitoring of people with specific diseases, under established protocols and clinical guidelines and within consolidated interdisciplinary health teams in a cost-effective way. One of the areas with the greatest coverage deficit in Latin America is adult oncology, with inequality in care opportunities for these patients. Part of the premature deaths attributable to this disease are due to the lack of access to timely diagnosis and treatment. As a contribution to the reduction of this gap, a training program of Advanced Nursing Practice was developed, addressing the most urgent needs in the field of oncology. The necessary competencies were defined to develop a professional-level master's degree program, considering prevention, early detection, diagnosis, treatment, and monitoring of people with oncological diseases at different levels of care. A program of this kind is an opportunity to reduce the access gap and coverage of health care for people with cancer, improving their quality of life and their survival.

Enzyme activities and microbial functional diversity in metal(loid) contaminated soils near to a copper smelter.

The monitoring of soil metal(loid) contamination is of global significance due to deleterious effects that metal(loid)s have on living organisms. Soil biological properties such as enzyme activities (EAs) are good indicators of metal(loid) contamination due to their high sensitivity, fast response, and low-cost. Here, the effect of metal(loid) contamination on physicochemical properties and microbial functionality in soils sampled from within 10 km of a Cu smelter is investigated. Soil composite samples were randomly taken within 2, 4, 6, 8 and10 km zones from a mining industry Cu smelter. The EAs of dehydrogenase (DHA), arylsulfatase (ARY), ß-glucosidase, urease, and arginine ammonification (AA) were studied as indicators of metal(loid) contamination, which included the ecological dose (ED50) with respect to Cu and As contents. The community level physiological profile (CLPP), functional diversity, and catabolic evenness were evaluated based on the C-substrate utilisation. All EAs decreased in zones with high degrees of metal(loid) contamination, which also had low TOC and clay contents, reflecting long term processes of soil degradation. Positive and strong relationships between EAs and TOC were found. DHA and ARY activities decreased by approximately 85-90% in highly metal(loid) contaminated soils. DHA and AA showed significant ED50 values associated with available Cu (112.8 and 121.6 mg CuDTPA kg-1, respectively) and total As contents (30.8 and 31.8 mg As kg-1, respectively). The CLPP showed different metabolic profiles along the metal(loid) contamination gradients. Long-term stress conditions in soils close to industrial areas resulted in the decreasing of general biological activity, catabolic capacity, and functional diversity.

Cytological diagnosis of Brunner's gland adenoma (hyperplasia): A diagnostic challenge.

Brunner's gland adenoma (hyperplasia) (BGA/H) is a benign gastrointestinal lesion, usually asymptomatic and frequently detected incidentally by endoscopy as a submucosal nodule. Most BGA/Hs are diagnosed by their typical cytological morphology and immunohistochemical features, characterized by monomorphic cells arranged as loosely clusters of epithelial cells with abundant, clear, and granular cytoplasm, eccentrically located nuclei, and immunoreactivity for MUC-6. The combination of the clinical and pathological features is essential for rendering a correct diagnosis. Herein, we report two cases of BGA/H, including their cytologic and histologic features, and a literature review of the clinicopathologic findings along with its differential diagnoses.

Spanish-Speaking Parents' Experiences Accessing Academic Medical Center Care: Barriers, Facilitators and Technology Use.

BACKGROUND AND OBJECTIVE: Children of Spanish-speaking caregivers face multiple barriers to care in academic medical centers. This study identified barriers and facilitators of health care and described use of health information technology in order to guide interventions and optimize services. METHODS: In-depth, audiotaped interviews were conducted with monolingual Spanish-speaking caregivers (N = 28) of children receiving care in academic medical center clinics using a structured interview guide. Interviews were transcribed in Spanish, and key themes were identified using thematic analysis. Illustrative quotes for each theme were translated into English. RESULTS: Language-specific barriers included arrival/registration occurring in English, lack of bilingual personnel, heavy reliance on interpreters, long wait times, and challenging phone communication. Non-language-specific barriers included medical center size and complexity, distance to services, lack of convenient and coordinated appointments, missing work/school, and financial barriers including insurance coverage or lack of citizenship. Caregivers identified interpreters, bilingual physicians and staff, and written materials in Spanish as facilitators of care. Most caregivers had internet access and expressed interest in health information technology, including patient portals, to communicate about their children's health. CONCLUSIONS: Caregivers of Spanish-speaking children encounter many language-specific barriers, which are compounded by non-language-specific barriers arising from complex health systems and social needs. Caregivers with limited resources described working hard to meet children's complex health care needs despite these barriers. Most caregivers had internet access and interest in patient portals. Academic medical centers may need multifaceted interventions that improve the availability of bilingual staff and interpreters and also address caregivers' social and informational needs.