Cannabidiol Modifies the Glutamate Over-Release in Brain Tissue of Patients and Rats with Epilepsy: A Pilot Study.

Drug-resistant epilepsy (DRE) is associated with high extracellular levels of glutamate. Studies support the idea that cannabidiol (CBD) decreases glutamate over-release. This study focused on investigating whether CBD reduces the evoked glutamate release in cortical synaptic terminals obtained from patients with DRE as well as in a preclinical model of epilepsy. Synaptic terminals (synaptosomes) were obtained from the epileptic neocortex of patients with drug-resistant temporal lobe epilepsy (DR-TLE, n = 10) or drug-resistant extratemporal lobe epilepsy (DR-ETLE, n = 10) submitted to epilepsy surgery. Synaptosomes highly purified by Percoll-sucrose density gradient were characterized by confocal microscopy and Western blot. Synaptosomes were used to estimate the high KCl (33 mM)-evoked glutamate release in the presence of CBD at different concentrations. Our results revealed responsive tissue obtained from seven patients with DR-TLE and seven patients with DR-ETLE. Responsive tissue showed lower glutamate release (p < 0.05) when incubated with CBD at low concentrations (less than 100 µM) but not at higher concentrations. Tissue that was non-responsive to CBD (DR-TLE, n = 3 and DR-ELTE, n = 3) showed high glutamate release despite CBD exposure at different concentrations. Simultaneously, a block of the human epileptic neocortex was used to determine its viability through whole-cell and extracellular electrophysiological recordings. The electrophysiological evaluations supported that the responsive and non-responsive human epileptic neocortices used in the present study exhibited proper neuronal viability and stability to acquire electrophysiological responses. We also investigated whether the subchronic administration of CBD could reduce glutamate over-release in a preclinical model of temporal lobe epilepsy. Administration of CBD (200 mg/kg, p.o. every 24 h for 7 days) to rats with lithium-pilocarpine-evoked spontaneous recurrent seizures reduced glutamate over-release in the hippocampus. The present study revealed that acute exposure to low concentrations of CBD can reduce the glutamate over-release in synaptic terminals obtained from some patients with DRE. This effect is also evident when applied subchronically in rats with spontaneous recurrent seizures. An important finding was the identification of a group of patients that were non-responsive to CBD effects. Future studies are essential to identify biomarkers of responsiveness to CBD to control DRE.

GABAB Receptors Tonically Inhibit Motoneurons and Neurotransmitter Release from Descending and Primary Afferent Fibers.

Motoneurons receive thousands of excitatory and inhibitory synapses from descending tracts and primary afferent fibers. The excitability of these neurons must be precisely regulated to respond adequately to the requirements of the environment. In this context, GABAA and GABAB receptors regulate motoneuron synaptic strength. GABAA and GABAB receptors are expressed on primary afferent fibers and motoneurons, while in the descending afferent fibers, only the GABAB receptors are expressed. However, it remains to be known where the GABA that activates them comes from since the GABAergic interneurons that make axo-axonic contacts with primary afferents have yet to be identified in the descending afferent terminals. Thus, the main aim of the present report was to investigate how GABAB receptors functionally modulate synaptic strength between Ia afferent fibers, excitatory and inhibitory descending fibers of the dorsolateral funiculus, and spinal motoneurons. Using intracellular recordings from the spinal cord of the turtle, we provide evidence that the GABAB receptor antagonist, CGP55845, not only prevents baclofen-induced depression of EPSPs but also increases motoneuron excitability and enhances the synaptic strength between the afferent fibers and motoneurons. The last action of CGP55845 was similar in excitatory and inhibitory descending afferents. Interestingly, the action of baclofen was more intense in the Ia primary afferents than in the descending afferents. Even more, CGP55845 reversed the EPSP depression induced by the increased concentration of ambient GABA produced by interneuron activation and GABA transporter blockade. Immunofluorescence data corroborated the expression of GABAB receptors in the turtle's spinal cord. These findings suggest that GABAB receptors are extrasynaptic and tonically activated on descending afferent fibers and motoneurons by GABA released from astrocytes and GABAergic interneurons in the cellular microenvironment. Finally, our results also suggest that the antispastic action of baclofen may be due to reduced synaptic strength between descending fibers and motoneurons.

Sistema de gestión TO-BE: garantizar el derecho a la salud de pacientes con ITS en Perú / TO-BE management system: guaranteeing the right to health of patients with STIs in Peru

Se realizó una investigación de campo para conocer la gestión de los procesos de solicitud y admisión de citas médicas, considerando la estigmatización y discriminación de las personas ITS, con el fin de garantizar el derecho a la salud de estos pacientes en Perú. Se ejecutó la sistematización de la información sobre los procesos y mecanismos de exigibilidad, bajo los lineamientos y manuales de los cuatro procesos estratégicos del MINSA contemplados para el otorgamiento de citas médicas, siguiendo los lineamientos de Gob.Pe, Adicionalmente, mediante verificación de los procesos se construyeron la matriz entidad-relación de la gestión por procesos del SSP, y el flujograma del proceso de solicitud y otorgamiento de citas AS-ISS. Se consideraron aspectos tecnológicos-médicos-legales con la participación de 201 pacientes independientemente del motivo o patología a consultar, identificando incidentes en la eficiencia del proceso misional. Por último, se implementó el flujograma de procesos de reserva de citas médicas a través de la aplicación ejecutable para smartphones, tabletas y otros dispositivos móviles (APP) TO-BE la cual conlleva a 10 pasos desde el registro de usuario hasta la emisión de comprobante de otorgamiento de cita médica. Se analizaron las preferencias de los usuarios sobre las causas superables en el proceso misional, mediante una encuesta estructura a 170 usuarios de SSP(AU)

A field investigation was carried out to learn about the management of the processes of request and admission of medical appointments, considering the stigmatization and discrimination of STI people, in order to guarantee the right to health of these patients in Peru. The systematization of the information on the processes and mechanisms of enforceability was carried out, under the guidelines and manuals of the four strategic processes of the MINSA contemplated for the granting of medical appointments, following the guidelines of Gov. Pe, Additionally, through verification of the processes The entity-relationship matrix of the management by processes of the SSP, and the flowchart of the process of request and granting of appointments AS-ISS were constructed. Technological-medical-legal aspects were considered with the participation of 201 patients regardless of the reason or pathology to be consulted, identifying incidents in the efficiency of the missionary process. Finally, the flowchart of medical appointment reservation processes was implemented through the executable application for smartphones, tablets and other mobile devices (APP) TO-BE, which entails 10 steps from user registration to issuance of voucher of granting a medical appointment. Users' preferences regarding causes that can be overcome in the missionary process were analyzed through a structured survey of 170 SSP users(AU)

Sars-CoV-2: impulsor del eGovernment en la administración de justicia en el distrito judicial de Junín / Sars-CoV-2: promoter of eGovernment in the administration of justice in the judicial district of Junín

El eGovernment es un sistema interconectado que interactúa con los ciudadanos y les brinda servicios aumentados a través de aplicaciones electrónicas, con la ayuda de las TIC, en este caso, con la llegada del Sars-CoV-2 se pretendió conocer el impulso en el sistema de justicia del distrito judicial de Junín, así como, las disposiciones para la contención de la propagación de este coronavirus emitidas por sistemas de justicia y salud; mediante el método Análisis- Síntesis. Se logró obtener que el 60.55% de los encuestados han manifestado que la implementación del Gobierno Electrónico coadyuva en la garantía en la impartición de justicia; coadyuva al cumplimiento del debido proceso y el 49.54% indicó estar de acuerdo en que la aplicación del eGovernment incide efectivizando la función jurisdiccional en el Distrito Judicial de Junín. A nivel nacional, se encuentra en pleno proceso de transformación digital a través de la identificación y adopción de soluciones digitales tales como la historia clínica electrónica, la telesalud y todas sus formas, y la Agenda Digital del Sector Salud 2020-2025, constituye un valioso instrumento para la articulación y conducción del sector, promoción de la transformación digital en salud con el uso de tecnologías de información para la mejora de calidad de la atención en salud y, la necesidad de acelerar dicha transformación, a fin de contribuir en mitigar los efectos de la emergencia sanitaria por la actual pandemia Covid-19 y, de otras que se presenten(AU)

The eGovernment is an interconnected system that interacts with citizens and provides them increased services through electronic applications, with the help of ICT, in this case, with the arrival of Sars-CoV-2, it was intended to know the momentum in the system of justice of the judicial district of Junín, as well as, the provisions for the containment of the spread of this coronavirus issued by justice and health systems; using the Analysis-Synthesis method. It was possible to obtain that 60.55% of the respondents have stated that the implementation of the Electronic Government contributes to the guarantee in the administration of justice; It contributes to compliance with due process and 49.54% indicated they agree that the application of the eGovernment affects the judicial function in the Junín Judicial District. At the national level, it is in the process of digital transformation through the identification and adoption of digital solutions such as electronic medical records, telehealth and all its forms, and the Digital Agenda of the Health Sector 2020-2025, constitutes a valuable instrument for the articulation and management of the sector, promotion of digital transformation in health with the use of information technologies to improve the quality of health care and, the need to accelerate said transformation, in order to contribute to mitigating the effects of the health emergency due to the current Covid-19 pandemic and, of others that arise(AU)

Asistencia sanitaria y servicios sociales: enseñanza y aprendizaje significativo en talleres técnicos por el método de proyecto / Healthcare and social services: meaningful teaching and learning in technical workshops by the project method

Los derechos humanos, incluyendo la concreción del derecho a la salud, en Perú, contempla el mandato dirigido a los poderes públicos para que organicen la asistencia sanitaria, como elemento causal, específico y adopten el conjunto de medidas necesarias para lograr la prevención de las enfermedades o la mejora de las condiciones sanitarias generales, mediante el método de proyecto en el proceso enseñanza-aprendizaje se abordan los principios rectores de la política social y económica, contenidos del Estado social, y los mecanismos de justiciabilidad y exigibilidad. Se estudió el proceso enseñanza y aprendizaje significativo sobre asistencia sanitaria y servicios sociales en los talleres técnicos, en la Facultad de Derecho y Ciencias Políticas, de la Universidad Peruana Los Andes, durante el periodo lectivo 2019-II. En el egresado conlleva la interacción entre los conocimientos y la información nueva que recibe, en este proceso se construye un nuevo conocimiento o profundiza en los ya existentes. Este conocimiento es organizado y sistemático con conexiones interdisciplinarias entre ideas, se promueve desde el trabajo en equipo y colaborativo, capaz de vencer ambigüedades, complejidades y a lo impredecible; y aprovecha los recursos o herramientas de la vida real, por lo que el método de proyecto es una herramienta pedagógica aplicable a la formación del abogado(AU)

Human rights, including the realization of the right to health, in Peru, contemplates the mandate directed to the public powers to organize health care, as a causal, specific element and adopt the set of measures necessary to achieve the prevention of diseases or the improvement of general sanitary conditions, through the project method in the teaching-learning process, the guiding principles of social and economic policy, contents of the social State, and the mechanisms of justiciability and enforceability are addressed. The teaching and meaningful learning process on health care and social services was studied in technical workshops at the Faculty of Law and Political Sciences, Universidad Peruana Los Andes, during the 2019-II school period. In the graduate, it involves the interaction between knowledge and the new information received, in this process a new knowledge is built or deepens existing ones. This knowledge is organized and systematic with interdisciplinary connections between ideas, it is promoted from team and collaborative work, capable of overcoming ambiguities, complexities and the unpredictable; and it takes advantage of the resources or tools of the real life, reason why the project method is a pedagogical tool applicable to the training of the lawyer(AU)

Interaction of NHE1 and TRPA1 Activity in DRG Neurons Isolated from Adult Rats and its Role in Inflammatory Nociception.

Transient receptor potential ankyrin 1 (TRPA1) channel is expressed in a subset of nociceptive neurons. This channel integrates several nociceptive signals. Particularly, it is modulated by intracellular pH (pHi). Na+/H+ exchanger 1 (NHE1) contributes to the maintenance of pHi in nociceptors. However, it is currently unknown whether the interaction between TRPA1 and NHE1 contributes to the nociceptive processing. Thus, the purpose of this study was to assess the functional interaction between NHE1 and TRPA1 in small dorsal root ganglion (DRG) neurons from primary culture obtained from adult rats. Moreover, we also evaluated their possible interaction in acute and inflammatory pain. Zoniporide (selective NHE1 inhibitor) reduced pHi and increased intracellular calcium in a concentration-dependent fashion in DRG neurons. Zoniporide and allyl isothiocyanate (AITC, TRPA1 agonist) increased calcium transients in the same DRG neuron, whereas that A-967079 (TRPA1 antagonist) prevented the effect of zoniporide in DRG neurons. Repeated AITC induced TRPA1 desensitization and this effect was prevented by zoniporide. Both NHE1 and TRPA1 were localized at the membrane surface of DRG neurons in culture. Local peripheral zoniporide enhanced AITC-induced pronociception and this effect was prevented by A-967079. Likewise, zoniporide potentiated Complete Freund's Adjuvant (CFA)-induced hypersensitivity, effect which was prevented by A-967079 in vivo. CFA paw injection increased TRPA1 and decresed NHE1 protein expression in DRG. These results suggest a functional interaction between NHE1 and TRPA1 in DRG neurons in vitro. Moreover, data suggest that this interaction participates in acute and inflamatory pain conditions in vivo.

Spinal TASK-1 and TASK-3 modulate inflammatory and neuropathic pain.

This study assessed the participation of spinal TWIK-related acid-sensitive K+ channels 1 and 3 (TASK-1 and TASK-3) in inflammatory (formalin test) and neuropathic (spinal nerve ligation, SNL) pain in rats. Intrathecal pre-treatment (-10 min) with the TASK-1 blocker ML365 or TASK-3 blocker PK-THPP, but not vehicle, enhanced in a dose-dependent manner 1% formalin-induced acute and long-lasting secondary mechanical allodynia and mechanical hyperalgesia in rats. In contrast, intrathecal pre-treatment with terbinafine, an activator of TASK-3, reduced formalin-induced flinching and allodynia/hyperalgesia. Both blockers and terbinafine had similar effects on female and male rats. In addition, intrathecal injection of ML365 or PK-THPP blocked the terbinafine-induced antiallodynic effect in neuropathic rats, but they did not modify baseline withdrawal threshold in naïve or sham-operated rats. TASK-1 and TASK-3 mRNA and protein were expressed in L4 and L5 dorsal root ganglia (DRG) and dorsal and ventral spinal cord of naïve animals. Interestingly, formalin injection increased TASK-1 expression in ipsilateral L5 DRG, but not in the spinal cord. Moreover, formalin injection transiently enhanced TASK-3 expression in ipsilateral L5 DRG and dorsal spinal cord. In contrast, SNL down-regulated TASK-3 expression in the ipsilateral L4 and L5 DRG but not in dorsal or ventral spinal cord, while SNL did not modify TASK-1 expression at any tissue. The pharmacological and molecular results suggest that TASK-1 and TASK-3 have a relevant antinociceptive role in inflammatory and neuropathic pain.

Blockade of anoctamin-1 in injured and uninjured nerves reduces neuropathic pain.

The aim of this study was to determine the participation of anoctamin-1 in 2 models of neuropathic pain in rats (L5/L6 spinal nerve ligation [SNL] and L5 spinal nerve transection [SNT]). SNL and SNT diminished withdrawal threshold in rats. Moreover, SNL up-regulated anoctamin-1 protein expression in injured L5 and uninjured L4 DRG whereas that it enhanced activating transcription factor 3 (ATF-3) and caspase-3 expression only in injured L5 DRG. In marked contrast, SNT enhanced ATF-3 and caspase-3, but not anoctamin-1, expression in injured L5 DRG but it did not modify anoctamin-1, ATF-3 nor caspase-3 expression in uninjured L4 DRG. Accordingly, repeated (3 times) intrathecal injection of the anoctamin-1 blocker T16Ainh-A01 (0.1-1 µg) or MONNA (1-10 µg) partially reverted SNL-induced mechanical allodynia in a dose-dependent manner. In contrast, anoctamin-1 blockers only produced a modest effect in SNT-induced mechanical allodynia. Interestingly, intrathecal injection of T16Ainh-A01 (1 µg) or MONNA (10 µg) prevented SNL-induced up-regulation of anoctamin-1, ATF-3 and caspase-3 in injured L5 DRG. Repeated intrathecal injection of T16Ainh-A01 or MONNA also reduced SNT-induced up-regulation of ATF-3 in injured L5 DRG. In contrast, T16Ainh-A01 and MONNA did not affect SNT-induced up-regulation of caspase-3 expression in L5 DRG. Likewise, gabapentin (100 µg) diminished SNL-induced up-regulation of anoctamin-1, ATF-3 and caspase-3 expression in injured L5 DRG. These data suggest that spinal anoctamin-1 in injured and uninjured DRG participates in the maintenance of neuropathic pain in rats. Our data also indicate that expression of anoctamin-1 in DRG is differentially regulated depending on the neuropathic pain model.

Resumen Reunión Nacional de la Red de Vivienda Saludable Proyecciones, logros y retos al culminar el 2014 / Report on the National Meeting of the Healthy Housing Network. Perspectives, achievements and challenges at the end of 2014

Se realiza una relatoría de lo acontecido en la Reunión Nacional de Vivienda Saludable 2014, para informar a los interesados en el tema de los logros y los retos del trabajo de la Red Cubana durante el año 2014 ylas proyecciones a futuro, cuyos miembros trazan para el próximo año(AU)

A report is provided on the National Meeting of the Healthy Housing Network 2014, with the purpose of informing those interested in the topic about the achievements and challenges of the Cuban Network in 2014, as well as the actions planned by its members for next year(AU)

Role of TRPV1 and ASIC3 in formalin-induced secondary allodynia and hyperalgesia.

BACKGROUND: In the present study we determined the role of transient receptor potential V1 channel (TRPV1) and acid-sensing ion channel 3 (ASIC3) in chronic nociception. METHODS: 1% formalin was used to produce long-lasting secondary allodynia and hyperalgesia in rats. Western blot was used to determine TRPV1 and ASIC3 expression in dorsal root ganglia. RESULTS: Peripheral ipsilateral, but not contralateral, pre-treatment (-10min) with the TRPV1 receptor antagonists capsazepine (0.03-0.3µM/paw) and A-784168 (0.01-1µM/paw) prevented 1% formalin-induced secondary mechanical allodynia and hyperalgesia in the ipsilateral and contralateral paws. Likewise, peripheral ipsilateral, but not contralateral, pre-treatment with the non-selective and selective ASIC3 blocker benzamil (0.1-10µM/paw) and APETx2 (0.02-2µM/paw), respectively, prevented 1% formalin-induced secondary mechanical allodynia and hyperalgesia in both paws. Peripheral ipsilateral post-treatment (day 6 after formalin injection) with capsazepine (0.03-0.3µM/paw) and A-784168 (0.01-1µM/paw) reversed 1% formalin-induced secondary mechanical allodynia and hyperalgesia in both paws. In addition, peripheral ipsilateral post-treatment with benzamil (0.1-10µM/paw) and APETx2 (0.02-2µM/paw), respectively, reversed 1% formalin-induced secondary mechanical allodynia and hyperalgesia in both paws. TRPV1 and ASIC3 proteins were expressed in dorsal root ganglion in normal conditions, and 1% formalin injection increased expression of both proteins in this location at 1 and 6 days compared to naive rats. CONCLUSIONS: Data suggest that TRPV1 and ASIC3 participate in the development and maintenance of long-lasting secondary allodynia and hyperalgesia induced by formalin in rats. The use of TRPV1 and ASIC3 antagonists by peripheral administration could prove useful to treat chronic pain.

Experiencias exitosas con escolares en programas de vigilancia entomológica no convencional en una comunidad del estado Cojedes, Venezuela / Successful Experience with students in programmes of non-conventional entomological surveillance in a rural community from Cojedes State, Venezuela

Motivated the resurgence of dengue and the emergence of chikungunya fever active entomological surveillance of triatomines in Cojedes state decreased dramatically. In this situation there is a need to incorporate communities unconventional entomological surveillance. To this end, conducting a pilot study in school Peasant Village Settlement Fundo Zamorano Paraima, Aroita sector in the municipality of San Juan Bautista Pao Cojedes state in Venezuela, to provide skills and abilities in the considered chipos capture and thus avoid the paralysis of entomological surveillance. The test results showed that predict good omens in the incorporation of the community in the work of entomological surveillance.

Motivado al recrudecimiento del dengue y a la emergencia de la fiebre de Chikungunya la vigilancia entomológica activa de los triatominos en el estado Cojedes disminuyó drásticamente. Ante esta situación se planteó la necesidad de incorporar a las comunidades a la vigilancia entomológica no convencional. A tal efecto, se consideró la realización de un estudio piloto en escolares del Asentamiento campesino Fundo Zamorano Pueblo Paraima, sector Aroita, ubicado en el municipio Pao de San Juan Bautista del estado Cojedes en Venezuela, con el fin de proporcionarles habilidades y destrezas en la captura de chipos y, de esta forma, evitar la paralización de la vigilancia entomológica. El ensayo arrojó resultados que permiten predecir buenos augurios en la incorporación de la comunidad en las labores de vigilancia entomológica.

Evidence for the participation of Ca(2+)-activated chloride channels in formalin-induced acute and chronic nociception.

In this study we determined the role of Ca(2+)-activated chloride channels (CaCC) in acute and chronic nociceptive responses elicited by 1% formalin. Formalin injection produced a typical pattern of flinching behavior for about 1h. Moreover, it produced secondary allodynia and hyperalgesia in the ipsilateral and contralateral paws for at least 6 days. Local peripheral and intrathecal pre-treatment (-10 min) with the non-selective and selective CaCC blockers niflumic acid and CaCCinh-A01, respectively, prevented formalin-induced flinching behavior mainly during phase 2 of the formalin test. Furthermore, niflumic acid and CaCCinh-A01 also prevented in a dose-dependent manner the long-lasting evoked secondary mechanical allodynia and hyperalgesia in the ipsilateral and contralateral paws. Moreover, local peripheral and intrathecal post-treatment (on day 6) with both CaCC blockers decreased the established formalin-induced secondary mechanical allodynia and hyperalgesia behavior in both paws. CaCC anoctamin-1 and bestrophin-1 were detected in the dorsal root ganglia. Formalin injection increased anoctamin-1, but not bestrophin-1 protein levels at 6 days. Intrathecal injection of the CaCC inhibitor CaCCinh-A01 prevented formalin-induced anoctamin-1 increase. Data suggest that peripheral and spinal CaCC, and particularly anoctamin-1, participates in the acute nociception induced by formalin as well as in the development and maintenance of secondary mechanical allodynia and hyperalgesia. Thus, CaCC activity contributes to neuronal excitability in the process of nociception induced by formalin.

Salud y resultados del aprendizaje en educandos de primaria del Consejo Popular Cayo Hueso, 2009 / Health and learning outcomes in elementary learners in Cayo Hueso Community Council, Havana in 2009

Se realizó un estudio que tuvo como objetivos caracterizar la morbilidad de los educandos de primaria del Consejo Popular Cayo Hueso, del Municipio Centro Habana, en el curso escolar 2008-2009, e identificar la relación entre la morbilidad encontrada y los resultados académicos de los educandos. El estudio fue complejo combinado de tipo transversal descriptivo y analítico (caso y control). Se estudiaron los educandos de primer a sexto grados de las seis instituciones educacionales de la enseñanza primaria del mencionado Consejo Popular. Para cumplir los objetivos propuestos se les realizó a todas las madres un cuestionario con preguntas que exploraron los antecedentes patológicos personales de los educandos. Se identificaron alteraciones del estado de salud que pudieron estar relacionadas con las condiciones ambientales de los hogares o las instituciones educacionales y constituir factores de riesgo para el aprendizaje. El retardo del desarrollo psicomotor y la inatención e hiperactividad fueron las alteraciones de salud que más se asociaron con estos resultados académicos. Se hacen recomendaciones

A study aiming to characterize the morbidity of primary students was conducted in Cayo Hueso Community Council, Havana, in the 2008-2009 school year. Also, the relationship between the found morbidity and students´ academic results was to be identified. This was a complex cross-sectional combination of descriptive and analytical (case and control) study. The students from the first to sixth years were sample to study from six different primary education institutions from this community council. In order to meet our objectives, a questionnaire exploring the personal medical history of these students was applied to all mothers. Health disorders were identified. They could be related to the environmental conditions of their homes or their educational institutions and be risk factors for learning. Psychomotor developmental delay, inattention and hyperactivity were the health disorders most associated with these academic results. Recommendations are made

Hábitos, características psicosociales y resultados del aprendizaje en educandos de primaria en"Cayo Hueso", La Habana / Habits, psychosocial features and results of learning in primary school students in "Cayo Hueso", La Habana

El Ministerio de Educación realiza acciones para lograr cumplir los objetivos de la Tercera Revolución Educacional; una es el proyecto multicéntrico, liderado por el Instituto Central de Ciencia Pedagógicas, "Aprendizaje para Todos", en el marco del cual se solicitó al Instituto Nacional de Higiene, Epidemiología y Microbiología realizar un estudio en el que se caracterizaran los hábitos de vida y los aspectos psicosociales de los educandos de primaria, según resultados de su aprendizaje, y se relacionaran los hábitos y las características psicosociales con sus resultados académicos. El estudio fue complejo combinado, de tipo transversal, descriptivo y analítico (caso y control), en los educandos de primer a sexto grados de las seis instituciones educacionales de la enseñanza primaria del Consejo Popular "Cayo Hueso", durante el curso escolar 2006-2007. Para cumplir los objetivos a todas las madres se les aplicó un cuestionario con preguntas acerca de la historia educacional de los hijos, sus hábitos y comportamientos. Se identificaron hábitos de vida y comportamientos inadecuados que predominaron en los educandos con problemas de aprendizaje, tales como: jugar en exceso, dormir tiempo insuficiente o con dificultades, las malas relaciones con la madre o el padre y convivientes, el comportamiento violento, intranquilo e impulsivo, estar triste sin razón, poco satisfecho con estudios y no soportar las frustraciones. Se asociaron como factor de riesgo a los problemas de aprendizaje: el juego en exceso, ser intranquilo e impulsivo, poco satisfecho con el estudio, dormir tiempo insuficiente, o con dificultades, no soportar frustraciones y el comportamiento violento y triste sin razón. Como factor protector se encontró leer en tiempo libre.

Ministry of Education take actions to fulfil the objectives of the Third Educational Revolution; one is the multicenter project leaded by the Central Institute of Educational Science, "Learning for Everybody" where authors requested to National Institute of Hygiene, Epidemiology and Microbiology the carrying out of an study to characterize the life habits and the psychosocial features of primary school students, according to the results of their learning and that are related to habits and the psychosocial features with the academic results. The study was complex, combined, cross sectional, descriptive and analytical (case-control) conducted in the first to sixth grades students from the six educational institutions of primary teaching of the above mentioned council during the school course 200-2007. To fulfill the objectives the mother filled a questionnaire with questions on the educational history of children, habits and behaviors. There were identified inappropriate life habits and behaviors with predominance in students with learning problems including: to play in excess, an insufficient sleep hours or with difficulties for it, bad relationships with parents and other family members, the violent behavior, restless and impulsive, to be sad without any reason, no satisfied with the studies and to hate the frustrations. The risk factors included the above mentioned situations. As a protector factor was to read in the spare time.

Condiciones ambientales del hogar y resultados académicos de educandos de primaria del Consejo Popular "Cayo Hueso" (2007) / Home environmental conditions and the academic results of primary school students from "Cayo Hueso" Popular Conceil (2007)

Se realizó un estudio que tuvo como objetivos caracterizar las condiciones ambientales de los hogares de los educandos de primaria del Consejo Popular "Cayo Hueso", del Municipio de Centro Habana, en el curso escolar 2006-2007, e identificar la relación entre las condiciones ambientales existentes y los resultados académicos obtenidos en ese período. El estudio fue complejo, combinado, de tipo transversal, descriptivo y analítico (caso y control). Se estudiaron los educandos de primer a sexto grados de las seis instituciones educacionales de la enseñanza primaria del Consejo Popular "Cayo Hueso", del municipio de Centro Habana, durante el curso escolar antes mencionado. Para cumplir los objetivos propuestos, a todas las madres se les aplicó un cuestionario con preguntas que exploraron la historia educacional de los hijos, sus hábitos y comportamientos. Los resultados se expresaron en porcentajes por instituciones educacionales. Se identificaron condiciones de vida inadecuadas en las viviendas de los educandos que pueden actuar como factores de riesgo a su salud o al aprendizaje. La ingestión de alcohol y el hábito de fumar dentro de las viviendas, así como el riesgo social en su entorno, fueron los hábitos inadecuados que más se relacionaron con los problemas de aprendizaje.

The objective of present study was to characterize the identified environmental conditions and the academic results of students. Study was complex and combined and cross-sectional, descriptive and analytical (case-control). The students from first to sixth degrees from the six educational institutions of primary education from the above mentioned council. To fulfill the proposed objectives, in all mothers we applied a questionnaire with questions to explore the educational history of theirs children, habits and behaviors. Results were expressed in percentages by educational institutions. The conditions of a inappropriate life at homes of students were identified that may be like risk factors for health of for learning. Alcohol ingestion and smoking at home, as well as the social risk of its environment, were the inappropriate habits more related to learning problems.

Estudio preliminar de la ictiofauna y los hábitats acuáticos del Río Bajo Paraguá, Santa Cruz, Bolivia / Preliminary study of fish fauna and aquatic habitats in the Lower Paraguá River, Santa Cruz, Bolivia

A fin de conocer la riqueza íctica del Río Bajo Paraguá (cuenca del Iténez o Guaporé) se realizaron colectas de peces y análisis físico-químicos del agua en tres localidades del norte de Santa Cruz, sobre el límite oeste del Parque Noel Kempff Mercado. Los muestreos duraron 5 días en cada sitio (Piso Firme, Florida y El Refugio) y en dos épocas del año (aguas altas: Abril-Mayo; aguas bajas: Septiembre). Utilizando diferentes artes de pesca se capturaron 4098 peces pertenecientes a 124 especies, de las cuales 35 fueron nuevos registros para el Parque Noel Kempff Mercado. De las 124 especies, el 56 por ciento correspondió al orden Characiformes, 22 por ciento a Siluriformes (con 34 spp), 15 por ciento a Perciformes (18 cíclidos), 5 por ciento a Gymnotiformes y 2 por ciento a Synbranchiformes, Rajiformes y Beloniformes. La riqueza de especies fue menor en el curso superior del río (Piso Firme: 90 especies, Florida: 71 especies y El Refugio: 23 especies), al igual que la concentración de oxígeno que en El Refugio alcanzó niveles críticos en la época de escaso caudal por la mayor cobertura de plantas flotantes. La proporción de nuevos registros geográficos y los interrogantes sobre distribución y abundancia de los peces del Rio Paraguá indican la necesidad de implementar monitoreos sistemáticos asociados a los planes de manejo de la zona.

With the aim of improving our knowledge about the fish fauna of the Lower Paragua River (Iténez or Guaporé basin), fish collections and physic-chemical analyses of water were conducted in three localities in northern Santa Cruz, on the western border of the Noel Kempff Mercado National Park. Sampling periods lasted 5 days on each of three sites (Piso Firme, Florida y El Refugio) and two seasons (high waters: April-May, low waters: September). By using various fishing methods, 4098 fish belonging to 124 species were collected, 35 of them were new geographic records for the National Park. From all the species, 56 percent belonged to the Characiformes, 22 percent to Siluriformes (34 spp.), 15 percent Perciformes (18 spp. of cichlids), 5 percent Gymnotiformes and 2 percent Synbranchiformes, Rajiformes and Beloniformes. Species richness was low in the upper course of the river (Piso Firme: 90 species, Florida: 71 species, El Refugio: 23 species) as well as oxygen levels which reached a critical minimum at El Refugio during low waters and increased cover of floating vegetation. The proportion of new geographic records and the uncertainties about fish distribution and abundance indicate the need to conduct monitoring actions associated to the management plans in the area.

Spatial components of bird diversity in the Andes of Colombia: implications for designing a regional reserve system.

Beta diversity, or the turnover in species composition among sampling sites in a region, is an important criterion for obtaining adequate representation of regional biodiversity in systems of protected areas. Recently, the additive model for partitioning regional (gamma) diversity (in opposition to the multiplicative model) has been proposed because it allows a direct measure of the contribution of beta diversity to gamma diversity. We determined avian beta diversity along latitudinal (among neighboring river drainages) and elevational axes in a 1347-km2 region on the western slope of the Central Cordillera of the Colombian Andes, where a regional system of protected areas is being designed. We then compared avian beta diversity between sites based on rapid versus long-term (>1 year) inventories and between fragmented sites versus continuous forest. Overall, beta diversity represented 63.1% of gamma diversity among 16 sites. Elevational differences in species composition accounted for 43.3% of regional diversity, whereas differences among drainages accounted for 19.8%. A complementary cluster analysis showed that sites grouped by elevational zones. Rapid inventories overestimated beta diversity because of sampling effects, but the effect was biologically small. Estimators of species richness derived from species accumulation curves provided a useful alternative to compensate for undersampling in short-term surveys. Forest fragmentation increased beta diversity because of differential local extinction of populations. Nevertheless, in our region, forest fragments contributed to gamma diversity because they contained complementary sets of species. More importantly, they contained populations of special-interest species. Although the region is relatively small, our analyses indicate that spatial differentiation of the biota is an important factor for deciding number and location of protected areas in the Andean region.

Aneurisma intracraneal en la infancia / Intracranial aneurysm in childhood

Los aneurismas intracraneales son una causa infrecuente de hemorragia subaracnoidea en la infancia. Se presenta un paciente diagnosticado y tratado quirúrgicamente en el Hospital Pediátrico Provincial Eduardo Agramonte Piña de Camagüey. Se presentan aspectos diferenciales de la hemorragia subaracnoidea aneurismática entre pacientes adultos y pediátricos.

Intracranial aneurysms are infrequent cause of subarachnoid hemorrhage in childhood. A case diagnosed and treated surgically at Eduardo Agramonte Pina Pediatric Provincial Hospital is presented. Differential aspects of the aneurysmatic subarachnoid hemorrage among adult and pediatric patients are shown.