RESUMO
Objective: The aim of this study was to determine by immunohistochemistry the presence and significance of p53 and bcl-2 proteins in oral lichen planus (OLP) and oral squamous cell carcinoma (OSCC). Study Design: We used 21 cases diagnosed as OLP 16 diagnosed as OSCC and four normal gingival biopsies taken from healthy patients were used as controls. Slides were processed for immunohistochemistry using anti-p53 and anti-bcl-2 monoclonal antibodies. Results:: We found p53 immunoexpression in 71.4 We found p53 immunoexpression in 71.4 We found p53 immunoexpression in 71.4(..) (AU)
Assuntos
Humanos , Proteína Supressora de Tumor p53/isolamento & purificação , Proteína Killer-Antagonista Homóloga a bcl-2/isolamento & purificação , Carcinoma de Células Escamosas/patologia , Líquen Plano Bucal/patologia , Lesões Pré-Cancerosas/patologia , Distúrbios no Reparo do DNA/patologiaRESUMO
OBJECTIVE: The aim of this study was to determine by immunohistochemistry the presence and significance of p53 and bcl-2 proteins in oral lichen planus (OLP) and oral squamous cell carcinoma (OSCC). STUDY DESIGN: We used 21 cases diagnosed as OLP 16 diagnosed as OSCC and four normal gingival biopsies taken from healthy patients were used as controls. Slides were processed for immunohistochemistry using anti-p53 and anti-bcl-2 monoclonal antibodies. RESULTS: We found p53 immunoexpression in 71.4% OLP cases and 68.7% OSCC cases, with no immunoexpression in control cases. Bcl-2 was negative for all OLP and OSCC cases, and mild positivity was observed in normal tissue. We found significant correlation among p53 expression and OSCC malignancy. CONCLUSIONS: Our results suggest that TP53 system mainly promotes a hyperproliferative state by cell cycle arrest of the OLP epithelial cells for repairing damaged DNA nor apoptosis and that anti-apoptotic action of bcl-2 is not important in this disease.
