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1.
Microbiol Spectr ; 11(6): e0217923, 2023 Dec 12.
Artigo em Inglês | MEDLINE | ID: mdl-37855460

RESUMO

IMPORTANCE: Nosocomiicoccus species are recently described as members of the Staphylococcaceae family. With their inclusion in commercial matrix-assisted laser desorption/ionization-time of flight mass spectrometry databases, Nosocomiicoccus species can now be identified when Gram-positive cocci in clusters are detected in positive blood cultures. However, their clinical significance is not known, making it difficult for the clinical microbiology laboratory to decide the extent of work-up. Based on our study, Nosocomiicoccus species demonstrate low pathogenicity and opportunistic potential. If isolated from a single blood culture set, limited work-up should be performed to an extent similar to other possible blood culture contaminants.


Assuntos
Bacteriemia , Hemocultura , Humanos , Relevância Clínica , Staphylococcaceae , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz/métodos , Bacteriemia/microbiologia
2.
Diagn Microbiol Infect Dis ; 101(4): 115534, 2021 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-34601446

RESUMO

Bloodstream infection with Bacillus cereus/thuringiensis can be life threatening, particularly in patients who are severely immunocompromised. In this report we describe a case that progressed from asymptomatic to fatal over approximately 5 hours despite extensive resuscitation efforts. We identify the pathogen and assemble its genome, in which we find genes for toxins that may have contributed to the precipitous demise. In the context of this and other cases we discuss the possible indication for rapid appropriate antibiotic administration and potentially antitoxin treatment or toxin removal in fulminant illness in immunocompromised patients.


Assuntos
Bacillus cereus/patogenicidade , Bacillus thuringiensis/patogenicidade , Bacillus cereus/genética , Bacillus cereus/isolamento & purificação , Bacillus thuringiensis/genética , Bacillus thuringiensis/isolamento & purificação , Toxinas Bacterianas/genética , Pré-Escolar , Evolução Fatal , Feminino , Genoma Bacteriano/genética , Infecções por Bactérias Gram-Positivas/imunologia , Infecções por Bactérias Gram-Positivas/microbiologia , Infecções por Bactérias Gram-Positivas/patologia , Humanos , Hospedeiro Imunocomprometido , Sepse/imunologia , Sepse/microbiologia , Sepse/patologia
3.
Open Forum Infect Dis ; 6(7)2019 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-31363766

RESUMO

Arcobacter spp. are commonly associated with shellfish and have been increasingly implicated in human gastrointestinal disease. We report the first case of human bacteremia with Malacobacter (previously Arcobacter) mytili acquired after exposure to Maryland crab. Arcobacter spp. should be considered in febrile illnesses when the history indicates exposure to seafood.

4.
Anaerobe ; 42: 98-100, 2016 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-27693544
7.
Am J Clin Pathol ; 145(1): 22-8, 2016 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-26712867

RESUMO

BACKGROUND: Corynebacterium species and gram-positive coryneform-like bacteria (coryneforms) are increasingly reported causes of opportunistic infections in immunocompromised patients. Biochemical identification methods for these bacteria are often inaccurate. We evaluated matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) for routine Corynebacterium and coryneform identification. METHODS: A total of 286 Corynebacterium species and coryneforms recovered from patients were identified by MALDI-TOF MS analysis using the Bruker Microflex instrument, Biotyper software version 3.0, and database version 3.1.66 (Bruker Daltonics, Billerica, MA) following formic acid-based, direct on-plate extraction. The spectral cutoff scores used for interpretation were 2.0 or more for species-level identification and 1.7 or more for genus level. Scores lower than 1.7 were considered as no reliable identification. The results were compared with API Coryne (bioMérieux, Durham, NC) and sequencing of 16S ribosomal RNA genes. RESULTS: Of the 231 Corynebacterium (19 species), 99.6% were correctly identified to the genus level and 88.7% to the species level. Of the 55 coryneforms (14 genera), 90.9% were correctly identified to the genus level and 67.3% to the species level. API Coryne was able to identify 89.2% of Corynebacterium species (species level) and 63.6% of coryneforms (genus level). CONCLUSIONS: Rapid on-plate testing yielded identification of more Corynebacterium species and related bacteria than biochemical methods.


Assuntos
Técnicas Bacteriológicas/métodos , Corynebacterium/isolamento & purificação , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz/métodos , Técnicas Bacteriológicas/normas , Humanos , Manejo de Espécimes/métodos , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz/normas
8.
J Clin Microbiol ; 53(12): 3926-30, 2015 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-26468502

RESUMO

During a 14-month period of using matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) for group B streptococcus (GBS) identification, we recovered 32 (1%) Streptococcus pseudoporcinus isolates from 3,276 GBS screening cultures from female genital sources (25 isolates from pregnant women and 7 from nonpregnant women). An additional two S. pseudoporcinus isolates were identified from a urine culture and a posthysterectomy wound culture. These isolates were found to cross-react with three different GBS antigen agglutination kits, PathoDx (Remel) (93%), Prolex (Pro-Lab Diagnostics) (38%), and Streptex (Remel) (53%). New approaches to bacterial identification in routine clinical microbiology laboratories may affect the prevalence of S. pseudoporcinus.


Assuntos
Técnicas Bacteriológicas/métodos , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz/métodos , Infecções Estreptocócicas/diagnóstico , Streptococcus/classificação , Streptococcus/isolamento & purificação , Adolescente , Adulto , Testes de Aglutinação , Feminino , Humanos , Gravidez , Estudos Prospectivos , Streptococcus/química , Adulto Jovem
12.
J Clin Microbiol ; 47(5): 1599-601, 2009 May.
Artigo em Inglês | MEDLINE | ID: mdl-19261780

RESUMO

We describe two cases of Clostridium glycolicum wound infections in immunocompetent adults. The bacterium was identified by 16S rRNA gene sequencing. This is the third published report of the recovery of this organism from human clinical material and highlights the importance of the organism as a potential human pathogen. Our report extends the spectrum of the diseases caused by C. glycolicum.


Assuntos
Infecções por Clostridium/diagnóstico , Clostridium/isolamento & purificação , Infecção dos Ferimentos/microbiologia , Adulto , Infecções por Clostridium/microbiologia , DNA Bacteriano/genética , DNA Ribossômico/genética , Feminino , Humanos , Masculino , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , Adulto Jovem
13.
Diagn Microbiol Infect Dis ; 61(4): 484-6, 2008 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-18482816
14.
Anaerobe ; 11(6): 350-3, 2005 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-16701598

RESUMO

Leptotrichia buccalis is rarely implicated in systemic disease. We report two patients with clinically significant L. buccalis bacteremia which developed during the neutropenia secondary to chemotherapy. Based upon our experience, L. buccalis bacteremia should be considered in certain high-risk immunocompromised patients with mucositis and/or gingivitis.

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