RESUMO
We studied the influence of extracellular vesicles from the follicular fluid of a young donor on gene expression (MKI67, MYBL2, CCNB1, CCND1, CCNE1, CALM2, BAX, NDRG1, TP53I3, VEGF, VCAN, HAS2, CTSL2, PIBF1, RPL37, PFKP, GPX3, and AQP3) in embryos of women of different ages. According to nanoparticle tracking analysis data, the concentration of extracellular vesicles was 3.75±0.47×1011 particles/ml and the mean particle size was 138.78±9.90 nm. During co-culturing of the follicular fluid extracellular vesicles with blastocysts of young women, we observed significantly increased expression of mRNA for genes CTSL2, CCND1, CCNE1, VEGF and reduced expression of BAX gene mRNA in comparison with embryos in women of late reproductive age. We hypothesized that addition of extracellular vesicles of the oocyte follicular fluid from a young donor to the culture medium of embryos could slow down apoptosis process typical of blastocyst cells in women above 36 years.
Assuntos
Apoptose , Blastocisto , Vesículas Extracelulares , Líquido Folicular , Humanos , Feminino , Vesículas Extracelulares/metabolismo , Vesículas Extracelulares/genética , Apoptose/genética , Adulto , Líquido Folicular/metabolismo , Blastocisto/metabolismo , Blastocisto/citologia , Regulação da Expressão Gênica no Desenvolvimento , Proliferação de Células , Oócitos/metabolismo , Fatores Etários , Desenvolvimento Embrionário/genética , RNA Mensageiro/genética , RNA Mensageiro/metabolismoRESUMO
The article demonstrates an algorithm to evaluate functional state of operators exposed to ecologic hazards. The evaluation of the functional state includes analysis of the heart rhythm registered for 2 minutes. The functional state is assessed through an Indicator of Regulatory Systems Activity (IRSA), obtained by summation of all the values in modulus. IRSA values define 5 functional states: norm; functional strain moderate, marked and extreme; asthenia of regulatory systems. The algorithm suggested differs from the known ones in some features as follows. The diagnosis of heterotopic arrhythmia serves for more precise code demonstrating the heart automatism; formed on the basis of variation coefficient, the code demonstrating stability of the regulation proves the status of stable or disordered regulation; the code demonstrating activities of subcortical neural centers is formed on the basis of measured relative capacity of primary and secondary respiratory waves.
