RESUMO
The horn extracts from the saiga of different age were used to investigate its effect on organotypic tissue culture of explants from the rat spleen. From 4 to 10 amino acids were detected in the different extract fractions. The horn extracts from the young saiga, at the effective concentration 1 ng/ml, showed stimulating effect in spleen tissue cultures as compared to the control explants. No effect of extracts from the old saiga was observed in tissue culture. Possibly, a stimulation of the cellular proliferation is due to the great amino acid content in saiga horn; however this phenomenon is decreased by the aging.
Assuntos
Cornos/química , Ruminantes/crescimento & desenvolvimento , Baço/efeitos dos fármacos , Extratos de Tecidos/farmacologia , Animais , Apoptose/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos , Cornos/crescimento & desenvolvimento , Técnicas de Cultura de Órgãos , Ratos , Ratos Wistar , Baço/citologia , Baço/imunologia , Baço/patologia , Extratos de Tecidos/isolamento & purificaçãoRESUMO
Saitarin (an extract from the horns of antelope Saiga) depresses complex instrumental drinking behavior of rats and induced specific changes in the structure of goal-directed behavioral acts. Such changes are to a certain extent suppressed by naloxone. The obtained evidence suggest that the effects of saitarin are caused by its predominant influence on the processes of reinforcement in the course of realization by animals of the learned behavioral acts.