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1.
Georgian Med News ; (348): 72-77, 2024 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-38807395

RESUMO

Abnormalities of the cytoskeleton and the slit diaphragm of podocytes have been attributed to diabetic nephropathy. In this study, we assessed urinary excretion of alpha-actinin-4 (ACTN-4), a cytoskeleton protein and a component of the slit diaphragm, and tight junction protein 1 (TJP-1, or ZO-1), a peripheral membrane protein that forms molecular complexes with actin filaments, in patients with type 2 diabetes (T2D) and albuminuric or non-albuminuric chronic kidney disease (CKD). The study included 140 patients with long-term T2D (≥10 years) and 20 healthy subjects as control. Patterns of CKD were identified based on the estimated glomerular filtration rate (eGFR) and urinary albumin-to-creatinine ratio (UACR). Urinary ACTN-4 and TJP-1 were assessed by ELISA. Patients with T2D had increased urinary excretion of ACTN-4 (p=0.03) and TJP-1 (p=0.006). In logistic regression models, both ACTN-4 and TJP-1 demonstrated associations with albuminuric CKD (UACR ≥3.0 mg/mmol and eGFR <60 mL/min×1.73 m2) after adjusting to age, sex, diabetes duration, HbA1c, and smoking. In ROC-analysis, TJP-1 excretion ≥70 pg/mmol was associated with albuminuric CKD (OR 5.45, 95% CI 1.96-15.18, p=0.001). The results demonstrate that elevated urinary ACTN-4 and TJP-1 are associated specifically with albuminuric CKD, but not with non-albuminuric CKD, in T2D patients.


Assuntos
Actinina , Diabetes Mellitus Tipo 2 , Taxa de Filtração Glomerular , Insuficiência Renal Crônica , Proteína da Zônula de Oclusão-1 , Humanos , Actinina/urina , Masculino , Diabetes Mellitus Tipo 2/urina , Feminino , Pessoa de Meia-Idade , Insuficiência Renal Crônica/urina , Insuficiência Renal Crônica/fisiopatologia , Proteína da Zônula de Oclusão-1/urina , Proteína da Zônula de Oclusão-1/metabolismo , Idoso , Nefropatias Diabéticas/urina , Nefropatias Diabéticas/fisiopatologia , Albuminúria/urina , Creatinina/urina , Estudos de Casos e Controles , Adulto
2.
Artigo em Russo | MEDLINE | ID: mdl-36385083

RESUMO

Despite the fact that the coronavirus pandemic has almost disappeared, its consequences will be a problem for national health systems, medical institutions and individual citizens for a long time to come. During the period of quarantine measures, which were the main form of social distancing and a measure to counteract the spread of the disease, many people practicing a healthy lifestyle, sports and just moderate physical activity were forced to significantly reduce their physical education practice. As a result, many of them faced the problem of weight gain.The article examines the quantitative and qualitative aspects of this problem through the prism of sociological research by international analytical agencies.


Assuntos
COVID-19 , Quarentena , Humanos , COVID-19/prevenção & controle , Aumento de Peso , Pandemias/prevenção & controle , Exercício Físico
3.
Bull Exp Biol Med ; 173(2): 229-234, 2022 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-35737161

RESUMO

We developed a new test system to detect the omicron variant of SARS-CoV-2 using allele-specific reverse transcription PCR and estimated the frequency of its detection in patients living in the Novosibirsk Region. Clinical samples were divided into 3 groups: samples collected from December 1 to December 30, 2021 (group 1; n=66), from December 30, 2021 to January 10, 2022 (group 2; n=20), and from January 11 to January 22, 2022 (group 3; n=101). Based on the identification of 5 mutations specific to SARS-CoV-2 (B.1.1.529), two systems of oligonucleotide primers and probes were developed for detecting this coronavirus genotype in clinical samples. Limit of detection (LOD95) was 4×103 genome equivalents per 1 ml of clinical sample for the first test system and 2×103 for the for the second test system. The omicron variant of SARS-CoV-2 was absent in group 1 of studied samples, but was detected in 20% (4/20) of group 2 samples and 88% of group 2 samples collected within less than 2 weeks of January 2022. Using developed test system, we showed that in less than 2 weeks the omicron variant has become dominant in patients, which confirms previously published data on its exceptional contagiousness.


Assuntos
COVID-19 , SARS-CoV-2 , COVID-19/diagnóstico , Humanos , RNA Viral , SARS-CoV-2/genética , Sensibilidade e Especificidade
4.
Bull Exp Biol Med ; 172(5): 649-652, 2022 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-35352258

RESUMO

The histamine content in bioamine-containing cells and the content of NSE+ cells of the lymph nodes were studied in rats in 40 min and in 2 and 4 h after bone marrow allotransplantation by using the cross luminescence-histochemical method and immunohistochemical method, respectively. Within 2 h after allotransplantation of the bone marrow, a significant increase in histamine content in bioamine-containing cells and an increase in the number of NSE+ cells in the period were observed. Both APUD and NSE+ cells were found to be heterogeneous by staining and luminescence.


Assuntos
Transplante de Medula Óssea , Histamina , Linfonodos , Animais , Células da Medula Óssea , Linfonodos/citologia , Neurônios , Fosfopiruvato Hidratase , Ratos
5.
Vavilovskii Zhurnal Genet Selektsii ; 25(8): 882-888, 2021 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-35083407

RESUMO

The objects of the study were recombinant clones of Komagataella phaffii K51 carrying the heterologous proteinase K (PK-w) gene from Tritirachium album integrated into their genome as well as samples of recombinant proteinase K isolated from these clones. The aims of this work were i) to determine whether it is possible to create recombinant K. phaffii K51 clones overexpressing functionally active proteinase K from T. album and ii) to analyze the enzymatic activity of the resulting recombinant enzyme. The following methods were used: computational analysis of primary structure of the proteinase K gene, molecular biological methods (PCR, electrophoresis of DNA in an agarose gel, electrophoresis of proteins in an SDS polyacrylamide gel under denaturing conditions, spectrophotometry, and quantitative assays of protease activity), and genetic engineering techniques (cloning and selection of genes in bacterial cells Escherichia coli TOP10 and in the methylotrophic yeast K. phaffii K51). The gene encoding natural proteinase K (PK-w) was designed and optimized for expression in K. phaffii K51. The proteinase K gene was synthesized and cloned within the plasmid pPICZα-A vector in E. coli TOP10 cells. The proteinase K gene was inserted into pPICZα-A in such a way that - at a subsequent stage of transfection into yeast cells - it was efficiently expressed under the control of the promoter and terminator of the AOX1 gene, and the product of the exogenous gene contained the signal peptide of the Saccharomyces cerevisiae a-factor to ensure the protein's secretion into the culture medium. The resultant recombinant plasmid (pPICZα-A/PK-w) was transfected into K. phaffii K51 cells. A recombinant K. phaffii K51 clone was obtained that carried the synthetic proteinase K gene and ensured its effective expression and secretion into the culture medium. An approximate productivity of the yeast recombinant clones for recombinant proteinase K was 25 µg/ mL after 4 days of cultivation. The resulting recombinant protease has a high specific proteolytic activity: ~5000 U/mg.

6.
Angiol Sosud Khir ; 26(4): 33-41, 2020.
Artigo em Russo | MEDLINE | ID: mdl-33332304

RESUMO

The article deals with the use of a genetically engineered drug for stimulation of angiogenesis as a component of combined treatment of complications of ischaemic form of diabetic foot syndrome, showing comparative results of using therapeutic angiogenesis in patients in whom it was impossible to perform operative revascularization of the affected extremity, also providing a detailed description of the methodology of combined treatment of pyonecrotic complications of diabetic foot syndrome. The study included a total of 62 patients with pyonecrotic complications of Wagner grade III-IV diabetic foot syndrome. The patients were divided into two groups. Group One patients after minor amputation on the foot were assigned to receive conventional basic therapy and topical treatment of the foot wound with antiseptics and modern dressings. Group Two patients, besides basic therapy, additionally received combined treatment which was carried out in two stages and included urokinase, sulodexide, and a venotonic agent. Local treatment of the foot wound was performed with the help of two-stage vacuum therapy. Both groups were further subdivided into subgroups A and B. The patients in subgroups B of both groups in order to prevent progression of limb ischaemia were additionally given Neovasculgen, a genetically engineered drug for stimulation of angiogenesis. The patients of subgroups A of both groups did not receive this drug. The immediate results were assessed on the 1st, 7th and 14th days of treatment by the dynamics of changes in subjective symptoms, cytograms of the wound surface, level of partial pressure of oxygen in capillary blood of foot tissues, and the necessity to perform repeated necrectomy. The remote results were evaluated by the dynamics of changes in the pain-free walking distance, maximum distance walked, ankle-brachial index, linear velocity of blood flow through tibial arteries and partial pressure of oxygen in capillary blood of the affected limb at the 6th, 12th and 36th months after the performed treatment, as well as by the limb salvage and patients' survival during 3 years.


Assuntos
Diabetes Mellitus , Pé Diabético , Amputação Cirúrgica , Pé Diabético/complicações , Pé Diabético/diagnóstico , Pé Diabético/cirurgia , Humanos , Isquemia/diagnóstico , Isquemia/etiologia , Isquemia/cirurgia , Salvamento de Membro , Período Pós-Operatório , Cicatrização
7.
Bull Exp Biol Med ; 170(2): 258-263, 2020 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-33263848

RESUMO

We studied the effect of bone marrow autotransplantation on the morphofunctional properties and numerical population of mast cells. The experiments were performed on 4-monthold male mice. The animals received an injection of a suspension of bone marrow obtained from the femoral epiphyses of these animals into the caudal vein. In 40 min and 2 h after autotransplantation, the number of tryptase-positive mast cells increased by 1.1 times. The formation of groups of mast cells near erythroid-neutrophil islets and near blood vessels was observed. The proportion of metachromatic mast cells significantly increased. By the degree of mast cells degranulation, we detected non-degranulated up to 48.0±1.4% (vs 55.2±1.2% in intact mice) and moderately degranulated mast cells 22.0±1.2% (vs 18.2±0.9% in intact mice); the percentage of actively degranulated cells was 10.0±0.8% (vs 3.6±0.9% in intact mice; p<0.05). Morphometric parameters of mast cells were changed, with a slight increase in their diameter and distance between the cells. The number of histamine-containing mast cells increased significantly (by 3.2 times in 40 min and by 5.9 times in 2 h) and histamine content in these cells also increased. Thus, bone marrow autotransplantation led to intensification of degranulation and sulfation of mast cells and the release of histamine from them.


Assuntos
Células da Medula Óssea/metabolismo , Heparina/química , Histamina/metabolismo , Mastócitos/metabolismo , Transplante Autólogo/métodos , Triptases/metabolismo , Animais , Medula Óssea/metabolismo , Diferenciação Celular , Proliferação de Células , Heparina/metabolismo , Masculino , Camundongos , Fatores de Tempo , Cloreto de Tolônio/química
8.
Mol Biol Rep ; 47(4): 3249, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-32072399

RESUMO

The original publication has been updated. The acknowledgment was omitted from the original article and is published below.

9.
Mol Biol Rep ; 47(1): 607-620, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31713007

RESUMO

Non-glycosylated, recombinant human granulocyte colony-stimulating factor (rhG-CSF), produced by Escherichia coli (filgrastim, leukostim) is widely used to treat a number of serious human diseases and aids in the recovery post bone marrow transplantation. Although glycosylation is not required for the manifestation of the biological activity of G-CSF, a number of studies have shown that the carbohydrate residue significantly increases the physicochemical stability of the G-CSF molecule. Therefore, the aim of the present study was to design a Pichia pastoris strain capable of producing glycosylated rhG-CSF, and to study its effects on rat bone marrow cells. The nucleotide sequence of the rhG-CSF gene has been optimized for expression in P. pastoris, synthesized, cloned into the pPICZαA vector and expressed under the control of the AOX promoter in P. pastoris X33. One of the selected clones secreting rhG-CSF, produced 100-120 mg/l of rhG-CSF three days post-induction with methanol. The recombinant cytokine was purified using two-step, ion-exchange chromatography. The final yield of purified G-CSF was 35 mg/L of culture medium. The biological activity of rhG-CSF was examined in rat bone marrow cells. The P. pastoris strain was designed to produce relatively high levels of rhG-CSF. The rhG-CSF protein had a strong stimulating effect on the growth of rat bone marrow cells, which was comparable to that of the commercial drug leukostim, but showed a more persistent effect on granulocyte cells and monocyte sprouts, enabling the enhanced maintenance of the viability of the cells into the 4th day of incubation.


Assuntos
Células da Medula Óssea , Fator Estimulador de Colônias de Granulócitos , Pichia/genética , Animais , Células da Medula Óssea/citologia , Células da Medula Óssea/efeitos dos fármacos , Células Cultivadas , Fator Estimulador de Colônias de Granulócitos/química , Fator Estimulador de Colônias de Granulócitos/genética , Fator Estimulador de Colônias de Granulócitos/isolamento & purificação , Fator Estimulador de Colônias de Granulócitos/farmacologia , Granulócitos/citologia , Granulócitos/efeitos dos fármacos , Ratos , Proteínas Recombinantes/química , Proteínas Recombinantes/genética , Proteínas Recombinantes/isolamento & purificação , Proteínas Recombinantes/farmacologia , Tíbia/citologia
10.
Int J Genomics ; 2019: 8173630, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31281830

RESUMO

The purpose of this work was to study changes in the level of cell-free DNA (cfDNA) in the blood of young and old rats in the normal state and with induced benign prostatic hyperplasia (BPH). Male Wistar rats were divided into 4 groups-young (3 months), old (20 months), intact, or with testosterone-induced BPH. Groups with BPH were subjected to surgical castration and administration of testosterone esters at a dose of 25 mg/kg for a total of 7 injections for 20 days. In intact animals, the level of cfDNA in old rats (2.00 ± 0.14 ng/µl) was significantly higher than that in the young (1.02 ± 0.30 ng/µl). The body and the prostate weights of old rats were 1.6 and 1.4 times larger than those of the young, without an increase in the prostate index (PI). The testosterone level in the blood of young rats was 1.6 times higher than that of old (6.20 ± 0.93 nmol/l vs. 3.77 ± 0.55 nmol/l; NS). In animals with BPH, the level of cfDNA in old rats (3.14 ± 0.76 ng/µl) was significantly higher than that in young rats (0.80 ± 0.14 ng/µl). The body and the prostate weights in old rats were 1.8 and 2.3 times larger, than those in young rats, with an increase in the PI. The level of testosterone in the blood of young (15.76 ± 0.51 nmol/l) and old (16.99 ± 1.1 nmol/l) rats was not significantly different. Morphological signs of BPH were observed in the prostate of both young and old rats. During the induction of BPH in the experiment, according to the level of cfDNA, cell death processes have not changed significantly in young rats but significantly increased in old rats. A similar trend was observed in the group of intact animals. The obtained data indicate that apoptosis processes are enhanced during the development of BPH despite the growth of tissues in the prostate itself.

11.
Appl Opt ; 57(10): C98-C102, 2018 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-29714273

RESUMO

A new method concerning secure free-space communications by means of chirped laser pulse interference is proposed. Physical-layer security of the link is ensured by encoding the data in a relative phase difference between two spatially separated beams. The possible architecture of the dual-node link is discussed based on acousto-optic elements for signal modulation and detection.

12.
Opt Express ; 26(2): 2117-2122, 2018 Jan 22.
Artigo em Inglês | MEDLINE | ID: mdl-29401936

RESUMO

The coloration of stainless steel surface due to the formation of spatially periodic structures induced by laser pulses of nanosecond duration is demonstrated. The period of microstructures corresponds to the laser wavelength, and their orientation angle depends on the adjustment of laser polarization. The marking algorithm for the development of authentication patterns is presented. Such patterns provide several levels of protection against falsification (visual, colorimetric and structural) along with high recording speed and capability of automated reading.

13.
Khirurgiia (Mosk) ; (10): 61-65, 2016.
Artigo em Russo | MEDLINE | ID: mdl-27804937

RESUMO

Venous thromboembolism is the major cause of death and important health problem. Anticoagulants is the mainstay modality of VTE treatment and rivaroxaban was the first novel anticoagulant approved for VTE treatment and secondary prevention in Russia. Current study was designed to assess efficacy and safety of rivaroxaban from the first day of VTE treatment. There were 78 patients in control group treated with LMWH and VKA, and 46 patients in the rivaroxaban group. Treatment duration was not less than 3 months. After 3 months there were 3 (3.85%) patients in control group and 1 (2.2%) patient in the rivaroxaban group with thrombus deterioration. Bleeding complications were observed in 6 (7.7%) patients in LMWH/VKA group and in 2 (4.3%) patients in the rivaroxaban group. CONCLUSION: results of the study show favorable benefit profile of rivaroxaban in VTE patients and rivaroxaban could be considered as initial therapy.


Assuntos
Hemorragia , Rivaroxabana , Tromboembolia Venosa/tratamento farmacológico , Angiografia por Tomografia Computadorizada/métodos , Esquema de Medicação , Monitoramento de Medicamentos/métodos , Inibidores do Fator Xa/administração & dosagem , Inibidores do Fator Xa/efeitos adversos , Feminino , Hemorragia/induzido quimicamente , Hemorragia/diagnóstico , Hemorragia/prevenção & controle , Humanos , Masculino , Pessoa de Meia-Idade , Estudos Prospectivos , Rivaroxabana/administração & dosagem , Rivaroxabana/efeitos adversos , Federação Russa , Tempo para o Tratamento , Resultado do Tratamento , Tromboembolia Venosa/diagnóstico
14.
Phys Rev E ; 93: 042708, 2016 04.
Artigo em Inglês | MEDLINE | ID: mdl-27176367

RESUMO

The multidimensional energy surface of a cholesteric liquid crystal in a planar cell is investigated as a function of spherical coordinates determining the director orientation. Minima on the energy surface correspond to the stable states with particular director distribution. External electric and magnetic fields deform the energy surface and positions of minima. It can lead to the transitions between states, known as the Fréedericksz effect. Transitions can be continuous or discontinuous depending on parameters of the liquid crystal which determine an energy surface. In a case of discontinuous transition when a barrier between stable states is comparable with the thermal energy, the activation transitions may occur, and it leads to the modification of characteristics of the Fréedericksz effect with temperature without explicit temperature dependencies of liquid crystal parameters. A minimum energy path between stable states on the energy surface for the Fréedericksz transition is found using the geodesic nudged elastic band method. Knowledge of this path, which has maximal statistical weight among all other paths, gives the information about a barrier between stable states and configuration of director orientation during the transition. It also allows one to estimate the stability of states with respect to the thermal fluctuations and their lifetime when the system is close to the Fréedericksz transition.

15.
Ter Arkh ; 87(6): 45-49, 2015.
Artigo em Russo | MEDLINE | ID: mdl-26281195

RESUMO

AIM: To study the relationship between serum inflammatory cytokine levels in chronic kidney disease patients with type 2 diabetes. SUBJECTS AND METHODS: Sixty-four patients aged 43 to 70 years with a glomerular filtration rate (GFR) of > 30 ml/min/1.73 m2 were examined. A control group consisted of 15 healthy individuals. The serum concentration of macrophage colony-stimulating factor (M-CSF), macrophage inflammatory protein 1α (MIP-1α), macrophage migration inhibitory factor (MIF), interleukin 6 (IL-6), as well as the urinary excretion of albumin and type IV collagen was determined by enzyme immunoassay. RESULTS: In patients with a GFR of > 60 ml/min/1.73 m2, M-CSF and MIF concentrations proved to be significantly higher than those in the control group (p = 0.0003 and p = 0.001, respectively). In those with a GFR of 30-59 ml/min/1.73 m2, there was an increase in the levels of M-CSF (p < 0.0001), MIP-1α (p = 0.002), MIF (p = 0.02), and IL-6 (p = 0.02). The decline in GFR was associated with the higher levels of M-CSF (p = 0.02) and MIP-1α (p = 0.02) and with the higher urinary excretion of type IV collagen (p = 0.01). M-CSF, MIP-1α, and IL-6 correlated positively with the urinary excretion of albumin (r = 0.34, r = 0.28, and r = 0.28, respectively; all p < 0.05) and type IV collagen (r = 0.31, r = 0.4, and r = 0.43, respectively; all p < 0.05). CONCLUSION: The findings confirm the concept that chronic inflammation is involved in the development of diabetic kidney disease.


Assuntos
Citocinas/sangue , Diabetes Mellitus Tipo 2/sangue , Inflamação/sangue , Insuficiência Renal Crônica/sangue , Adulto , Idoso , Biomarcadores/sangue , Diabetes Mellitus Tipo 2/complicações , Progressão da Doença , Feminino , Taxa de Filtração Glomerular , Humanos , Inflamação/complicações , Masculino , Pessoa de Meia-Idade , Insuficiência Renal Crônica/complicações , Insuficiência Renal Crônica/fisiopatologia
16.
Haemophilia ; 21(6): 791-8, 2015 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-26010678

RESUMO

INTRODUCTION: Antihaemophilic factor (recombinant), plasma/albumin-free method (rAHF-PFM) is a human recombinant full-length factor VIII (FVIII) approved worldwide for the control and prevention of bleeding episodes, routine prophylaxis and perioperative management in adults and children with haemophilia A. AIM: To evaluate rAHF-PFM safety [including adverse events (AEs) and inhibitor incidence] from 12 interventional studies spanning >10 years. METHODS: The study population comprised 418 treated patients (median age = 18.7 years) with FVIII levels ≤2% of normal, including 55 previously untreated or minimally treated patients (PUPs/MTPs) from all rAHF-PFM phase I-IV studies, excluding observational safety studies. RESULTS: Most AEs were non-serious; only 93 AEs in 45 patients (10.8%) were related to rAHF-PFM. A total of 106 serious AEs (SAEs) occurred in 69 patients (16.5%); the most common were FVIII inhibitors (4.1%), device-related infection (1.0%) and pyrexia (0.7%). The 17 SAEs considered related to treatment consisted of FVIII inhibitors in 1 previously treated patient (PTP) (≤5 Bethesda Units [BU]) and 16 PUPs/MTPs [7/55 high titre (>5 BU), 12.7%; 9/55 low titre (≤5 BU), 16.4%]. Overall, the incidence of FVIII inhibitors was 0.36% in PTPs and 29.1% in PUPs/MTPs. No deaths or cases of hypersensitivity related to rAHF-PFM occurred. CONCLUSION: This integrated safety analysis evaluated the safety and tolerability of rAHF-PFM in children and adults with moderately severe or severe haemophilia A in all interventional studies completed to date. It was important to review consolidated evidence as some AEs are rare. There were no new safety signals in a wide variety of clinical settings.


Assuntos
Ensaios Clínicos como Assunto , Fator VIII/uso terapêutico , Hemofilia A/tratamento farmacológico , Proteínas Recombinantes/uso terapêutico , Segurança , Adolescente , Adulto , Criança , Pré-Escolar , Fator VIII/efeitos adversos , Fator VIII/antagonistas & inibidores , Fator VIII/farmacocinética , Humanos , Lactente , Proteínas Recombinantes/efeitos adversos , Proteínas Recombinantes/farmacocinética
17.
Phys Rev E Stat Nonlin Soft Matter Phys ; 90(5-1): 052502, 2014 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-25493805

RESUMO

The contribution to the free energy of distortion of the ferroelectric smectic-C^{*} due to the electrostatic interaction of polarization charges is calculated. These calculations are performed by accounting for the anisotropy of the permittivity, which is essential for smectic-C^{*}. Fluctuations of the c director in an external electric field are considered. It is shown that the anisotropy of the permittivity strongly affects the interaction of the polarization charges, the spectrum orientation fluctuations, and the angular dependence of the light scattering intensity.

18.
Artigo em Inglês | MEDLINE | ID: mdl-25353814

RESUMO

We consider the multiple scattering of light by fluctuations of the director in a nematic liquid crystal. Using methods of numerical simulation the peak of the coherent backscattering and the coefficients of anisotropic diffusion are calculated. The calculations were carried out without simplifying assumptions on the properties of the liquid crystal. The process of multiple scattering was simulated as a random walk of photons in the medium. We investigated in detail the transition to the diffusion regime. The dependence of the diffusion coefficients on the applied magnetic field and the wavelength of light were studied. The results of simulation showed a nonmonotonic dependence of the diffusion coefficients on the external magnetic field. For calculation of the peak of the coherent backscattering we used the semianalytical approach as long as in nematic liquid crystals this peak is extremely narrow. The parameters of the backscattering peak and of diffusion coefficients which were found in numerical simulations were compared with the experimental data and the results of analytical calculation.

19.
Haemophilia ; 20(6): 777-83, 2014 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-25039809

RESUMO

UNLABELLED: A Post-Authorization Safety Study (PASS) global program was designed to assess safety and effectiveness of rAHF-PFM (ADVATE) use in haemophilia patients in routine clinical settings. The main aim of this project was to estimate the rate of inhibitors and other adverse events across ADVATE-PASS studies by meta-analysing individual patient data (IPD). Eligible Studies: PASS studies conducted in different countries, between 2003 and 2013, for which IPD were provided. Eligible patients: haemophilia A patients with baseline FVIII:C < 5%, with a known number of prior exposure days (EDs). PRIMARY OUTCOME: de novo inhibitors in severe, previously treated patients (PTPs) with > 150 EDs. SECONDARY OUTCOMES: de novo inhibitors according to prior exposure and disease severity; other adverse events; annualized bleeding rate (ABR). ANALYSIS: random-effects logistic regression. Five of seven registered ADVATE-PASS (Australia, Europe, Japan, Italy and USA) and 1188 patients were included (median follow-up 384 days). Among severe PTPs with > 150 EDs, 1/669 developed de novo inhibitors (1.5 per 1000; 95% confidence interval [CI] 0.2, 10.6 per 1000). Among all patients included in the PASS studies, 21 developed any type of inhibitors (2.0%, 95% CI: 0.8%, 4.7%). Less than 1% of patients presented with other serious adverse events possibly related to ADVATE. The overall median ABR was 3.83 bleeds/year (first, third quartiles: 0.60, 12.90); 1.66 (0, 4.78) in the 557 patients continuously on prophylaxis ≥ twice/week. Meta-analysing PASS data from different countries confirmed the overall favourable safety and effectiveness profile of ADVATE in routine clinical settings.


Assuntos
Fator VIII/uso terapêutico , Hemofilia A/tratamento farmacológico , Proteínas Recombinantes/uso terapêutico , Inibidores dos Fatores de Coagulação Sanguínea , Fator VIII/administração & dosagem , Fator VIII/efeitos adversos , Hemofilia A/complicações , Hemofilia A/diagnóstico , Hemorragia/etiologia , Humanos , Masculino , Vigilância de Produtos Comercializados , Proteínas Recombinantes/administração & dosagem , Proteínas Recombinantes/efeitos adversos , Índice de Gravidade de Doença , Resultado do Tratamento
20.
Klin Khir ; (5): 12-5, 2014 May.
Artigo em Ucraniano | MEDLINE | ID: mdl-25675756

RESUMO

The dynamics of indices of the carbohydrate metabolism was studied in 164 patients, suffering morbid obesity (MO) (body mass index--BMI over 40 kg/M2), before and in 6 mo after conservative and surgical treatment. In 81 patients (I group) for treatment of MO a diet and medicine "Styfimol" were used, and in 83 (II group)--bariatric operations (gastric banding or shunting). In 6 mo after the treatment in patients of both groups a trustworthy reduction of body mass and BMI was observed, including in a group I--by 5.5 kg (4.6%), and in a group II--by 35 kg (22.8%). As a consequence of this a level of insulin and a HOMA index was noted, what have had promoted a reduction of a revealing rate of glucose tolerance violation (GTV) and diabetes mellitus (DM) type II. More essential changes in carbohydrate metabolism were observed in a group II, including a rate of revealing of HGTV--by 41%, DM type II--by 75%.


Assuntos
Cirurgia Bariátrica , Restrição Calórica , Carboidratos da Dieta/administração & dosagem , Gastroplastia , Obesidade Mórbida/sangue , Adulto , Glicemia/metabolismo , Índice de Massa Corporal , Feminino , Seguimentos , Humanos , Insulina/sangue , Masculino , Obesidade Mórbida/dietoterapia , Obesidade Mórbida/fisiopatologia , Obesidade Mórbida/cirurgia , Redução de Peso
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