RESUMO
The strain Pseudomonas aeruginosa 142 isolated from the utilising PSBs bacterial association was capable of growth on 2-chloro- and 2,4-dichlorobenzoates as sole carbon sources, but it did not utilize 3-Cl, 4-Cl, 3,5-diCl- and 2,6-dichlorobenzoates. P. aeruginosa 142 dehalogenated 2-Cl-, 2,4-diCl- and 2,5-dichlorobenzoates in aerobic conditions. The release of chloride was not observed in microaerophilic and anaerobic conditions. The activities of catechol-1,2-dioxygenase and 4-chlorocatechol-1,2-dioxygenase were found in cell extracts after growth of this strain on 2,4-dichlorobenzoate. The presented results suggested that oxidative release of chloride in ortho-position is the first step of metabolism of 2-Cl-, 2,4-diCl- and 2,5-dichlorobenzoates. The further splitting of corresponding catechols is carried out by ortho-pathway.
Assuntos
Cloro/metabolismo , Clorobenzoatos/metabolismo , Dioxigenases , Pseudomonas aeruginosa/metabolismo , Aerobiose/fisiologia , Catecol 1,2-Dioxigenase , Catecóis/química , Estrutura Molecular , Oxirredução , Oxigenases/química , Oxigenases/isolamento & purificaçãoRESUMO
Inactivated whole-virion and split-virion influenza vaccines adsorbed on aluminium hydroxide did not suppress the immune system of the vaccinees. Immunization with these vaccine preparations induced the formation of both cell-mediated and humoral immunity in over 60% of the vaccinees.
Assuntos
Imunização , Vacinas contra Influenza/imunologia , Adsorção , Formação de Anticorpos , Linfócitos B/imunologia , Humanos , Vírus da Influenza A/imunologia , Contagem de Leucócitos , Linfócitos T/imunologia , Fatores de Tempo , Vacinas Atenuadas/imunologia , Vírion/imunologiaRESUMO
Live influenza vaccines prepared from influenza virus strains H/32/5 (H1N1) and H/1/2/17 (H3N2) induce the formation of humoral immunity only in a part of the vaccinees and cause the transitory quantitative deficiency of T-lymphocytes in their blood, resulting in the poorly pronounced booster effect of the subsequent revaccination.
Assuntos
Vacinas contra Influenza/imunologia , Linfócitos/imunologia , Anticorpos Antivirais/análise , Formação de Anticorpos , Especificidade de Anticorpos , Humanos , Imunidade Celular , Vírus da Influenza A/imunologia , Vírus da Influenza B/imunologia , Contagem de Leucócitos , Fatores de TempoRESUMO
The level and dynamics of lymphocyte blastogenesis in response to phytohaemagglutinin (PHA) and to specific influenza virus antigen were studied in 3 groups of humans, vaccinated with live or inactivated whole virion influenza vaccines (H3N2 type) and placebo (control group). Both live and inactivated influenza vaccines did not change significantly the functional activity of T lymphocytes as determined by the mean values of stimulation index (SI). The analysis of individual values of PHA-dependent blastogenic response, however, revealed a decrease in SI as compared with its prevaccination level in 33.3 +/- 11.4% of the vaccinees given the live influenza vaccine.
Assuntos
Vacinas contra Influenza/imunologia , Ativação Linfocitária , Adolescente , Adulto , Formação de Anticorpos , Humanos , Imunidade Celular , Vacinação , Vacinas AtenuadasRESUMO
The indices of cellular and humoral immunity (the number of leukocytes, lymphocytes, T- and B cells, E- and EAC-RFC, blastogenic reaction of lymphocytes in response to treatment with phytohemagglutinin and influenza virus antigen in vitro, antihemagglutinin and influenza virus antigen in vitro, antihemmagglutinin titres) in humans vaccinated with different types of inactivated influenza vaccines produced in the USSR and the GDR were investigated. The immunogenic potency, general and immunological innocuity of the inactivated influenza vaccines under study and more marked stimulation of the B-system of immunity by them were demonstrated.
Assuntos
Vacinas contra Influenza/administração & dosagem , Influenza Humana/imunologia , Adolescente , Adulto , Formação de Anticorpos/efeitos dos fármacos , Avaliação de Medicamentos , Alemanha Oriental , Homeostase/efeitos dos fármacos , Humanos , Imunidade Celular/efeitos dos fármacos , Influenza Humana/prevenção & controle , Contagem de Leucócitos , Ativação Linfocitária/efeitos dos fármacos , Fatores de Tempo , U.R.S.S. , Vacinas Atenuadas/administração & dosagemAssuntos
Reservatórios de Doenças , Infecções Meningocócicas/prevenção & controle , Portador Sadio/tratamento farmacológico , Humanos , Meningite Meningocócica/prevenção & controle , Medicina Militar , Penicilina G Benzatina/administração & dosagem , Penicilina G Benzatina/análogos & derivados , Sepse/prevenção & controle , U.R.S.S.RESUMO
Aerosol and intranasal methods of influenza vaccination were studied on volunteers. Aerosol vaccination induced intensive S-IgA-producing reaction and increase of the specific antibody titres in the saliva and nasal secretions. Intranasal vaccination led to increase of influenza antibodies in the washings from the nasopharynx. The presence of a wide spectrum of antiviral and antibacterial normal secretory antibodies was revealed in the fluids under study. Along with stimulation of specific secretory antibodies, the methods of influenza vaccination under study led to increase of the titre of antibodies nonspecific of the vaccine used.
