[Operative isolated chemohyperthermal perfusion of the lung with ablation of pulmonary metastases].

The authors have got their first experience with chemohyperthermal perfusion of the lung in 4 patients. The method and technique of the operation of ablation of metastases in the lungs with preliminary chemohyperthermal perfusion are described. All the patients survived the operative interventions.

[Adaptive effects of hypoxic preconditioning in brain neurons]. / Adaptivnye éffekty gipoksicheskogo prekonditsionirovaniia neironov mozga.

A preventive short-term hypoxia (preconditioning) increases neuronal resistance against subsequent strong hypoxic effects. Literature review and authors' own data on molecular-cellular mechanisms of the hypoxic preconditioning, are presented. Participation of intracellular signal transduction, genome, stress-proteins, and neuromodulating peptides in this process, is discussed. The role of glutamatergic as well as calcium and phosphoinositide regulatory systems and neuromodulating factors as the components of a "volume" signal transmission are analyzed in hypoxic precondition-associated induction of functional tolerance mechanisms against acute harmful effects in neurones of olfactory slices.

[Effect of fractions of the cerebrospinal fluid from patients with drug dependence on basic electrophysiological characteristics of the rat olfactory cortex slices]. / Issledovanie éffektov otdel'nykh fraktsii likvora bol'nykh narkoticheskoi zavisimost'iu na bazisnye élektrofiziologicheskie kharakteristiki perezhivaiushchikh srezov kory mozga krys.

Heroin addicts at the initial stage of abstinence syndrome were subjected to detoxication by liquorosorption technique. The fractions of their cerebrospinal fluid obtained by the thin layer chromatography technique were analyzed. The substances extracted from the cerebrospinal fluid of drug addicts, presumably peptides, negatively affected the conductive function and synaptic transmission in surviving slices of the olfactory cortex of rats. The conclusion was drawn about a possibility of application of surviving rat brain slices as test object for estimation of the extent of purification of the cerebrospinal fluid from toxic endogenous substances after the liquorosorption.

[Involvement of glutamate receptors (NMDA type) in reaction of brain neurons to anoxia of different duration]. / Vovlecheniie glutamatnykh retseptorov (NMDA-tipa) v reaktsii neironov mozga na anoksiiu razlichnoi dlitel'nosti.

The participation of NMDA receptors in the changes caused by evoked EPSPs in the activity of calcium and phophoinositide intracellular regulatory systems (ICRS) bioelectric activity (evoked EPSPs) and in cell production of polypeptides was studied in rat olfactory cortical slices exposed to short-term anoxia (STA) and long-term anoxia (LTA). To determine NMDA receptor involvement in these functional and metabolic reactions, the slices were treated with the antagonists APV and MK-801 during anoxia of different duration (STA and LTA being 2 and 10 min, respectively) and subsequent reoxygenation. EPSPs were recorded extracellularly in response to lateral olfactory tract stimulation. The STA-mediated stimulation of NMDA receptors was found to promote adaptive reactions (moderate activation of IRS, long-term potentiation of synaptic transmission) in the postanoxic period. LTA induced the long-lasting stimulation of NMDA receptors that led to the hyperactivation of calcium and phosphoinositide IRS, to the suppression of generation of EPSPs and of synthesis of polypeptides probably of adaptive action. All these events induced pathological processes in the postanoxic period.

[Modulatory effects of various factors of muscular origin on the function of the motor nerve endings]. / O moduliruiushchem deistvii nekotorykh faktorov myshechnogo proiskhozhdeniia na funktsiiu motornogo nervnogo okonchaniia.

Muscle incubate was shown to contain factors capable to increase the transmitter release in low-effective synapses and decrease it in high-effective those, dueto activation and inhibition, respectively, of the processes governing formation of available store of the transmitter. Activatiory effect of the low-molecular fraction on the low-effective synapses was correlated with the concentration of histidine-containing substances in this fraction.

[Biochemical characteristics of a muscle perfusate and its effects on mediator release in the neuromuscular synapse]. / Nekotorye biokhimicheskie kharakteristiki myshechnogo perfuzata i ego vliianie na osvobozhdenie mediatora v nervno-myshechnom sinapse.

Histidine-containing compounds (HCC) were found, using diazoreaction and reaction with diethylpyrocarbonate, a specific histidine reagent, in the perfusate of the frog hindlimb vasculature. After gel filtration on sephadex G-25 about 80% of HCC were eluted with a fraction with molecular mass less than 5000 kD. The studies of the perfusate influence on the characteristics of quantum secretion of transmitter in the preparation of frog cutaneopectoral muscle have shown that the perfusate increased the quantum content of end-plate potentials (EPP) due to increasing binominal parameter and decreasing frequency of miniature EPP. The characteristics of presynaptic action of the perfusate were similar to those of exogenous histidine.