[Examination of gastric juice in animals fed with fodder containing power plant ashes and loess dusts]. / Badania soku zoladkowego zwierzat karmionych pasza z zawartoscia popiolów elektrownianych i pylów lessowych.

Normal metabolism, regulation of stimulation and checking of electrolyte secretion in the gastric juice depend on the way of feeding and its nutritional ingredients. Feeding experimental animals with fodder containing power plant ashes or loess dusts causes a change in the gastric juice reaction, thus resulting in a different assimilation of elements contained in the fodder. There was shown an increase of some elements content (Mg, Na, Cu, Fe), a decrease in the content of some others (Pb,Zn) in the gastric juice and a positive tendency for blocking by calcium ions of heavy metals contained in the fodder by the mucous membrane of the stomach.

[Study of rat behavior in a feeding experiment]. / Badanie zachowania sie szczurów w doswiadczeniu zywieniowym.

The examination studied behaviour of rats fed on natritive material containing soil dust or electroenergetic ashes. Food containing the dust or ashes was administered to animals in the amount of 30 g a day for 26 weeks. Body weight was controlled in all animals and their motor activity, motor coordination and cognitive activity were examined. It has been settled that rats fed an food with the soil dust supplement showed higher motor activity and acquired greater motor coordination than animals fed either on food with the electroenergetic ashes supplement or on conventional food. During the first weeks of experiment, cognitive activity was getting lower in animals in both experimental groups and control group, and then only in the group of animals fed on food with the soil dust supplement.

[Histoenzymatic picture of rat gastric mucosa under the influence of dexamethasone]. / Obraz histoenzymatyczny blony sluzowej zoladka szczura znajdujacego sie pod wplywem Dexamethasonu.

White male [correction of female] rats were given Dexamethasone in a single dose 0.35 mg/24 hrs. Group I was given the drug once, group II--received it for 2 days, and group III--for 7 days. 24 hrs after the medicine had been given the mucous membrane of the stomach corpus was collected for examinations. There were performed: H+E staining, PAS reaction and histochemical reaction to the activity of ATP-ase, acid phosphatase and alkaline phosphatase. The results obtained were compared with those obtained in a group of control animals which were given distilled water. The administration of Dexamethasone did not affect significantly the activity of the examined hydrolases, however, it damaged the mucous barrier of the stomach, specially in the animals after 7 days of application of the drug.

[Histochemical and histologic examination of kidney from white rats after experimental administration of dexamethasone]. / Badania histochemiczne i histologiczne nerki szczura bialego po doswiadczalnym podawaniu dexamethasonu.

Kidneys of the rats which had been intragastrically administered Dexamethasone (10 mg/kg), were examined. Observations were carried out after a single and twice repeated application of the preparation, as well as after 7 days of everyday administration. It was found, on the basis of histological and histochemical observations (reaction to alkaline phosphatase activity, reaction to acid phosphatase activity, PAS-method staining), that both single and twice administration of Dexamethasone do not cause the damage of the kidney parenchyma, whereas after 7 days of everyday application of the preparation irreversible changes can be observed.

[Morphological evaluation and analysis of trace elements in the liver of rats who were fed with feed containing power station ashes]. / Morphologische Beurteilung und Analyse von Spurenelementen in der Leber von Ratten, die mit Kraftwerk-Aschen enthaltendem Futter gefüttert wurden.

The experiment was carried out on white Wistar rats. The rats in the experimental groups received fodder containing power station ashes for various periods of time (from 6 to 18 weeks). During the experiment, the body weight of rats was recorded, the amount of trace elements in the liver was estimated, and morphological tests of the liver were carried out. The results of these experiments indicate that feeding the rats on fodder containing power station ashes causes several percent increase in the body weight and slight differences in accumulation of trace elements. Morphological changes in the liver are connected with the vacuolization of the hepatocytes' cytoplasma and an increase in the amount of phagocytic cells in hepatic sinuses. The intensity of morphological changes was correlated with the time of experiment.

[Effect of diet containing soil dust on morphological examination of liver in experimental rats]. / Wplyw diety zawierajacej pyly glebowe na obraz morfologiczny watroby szczurów doswiadczalnych.

Investigations refer to the influence of fodder containing soil dust as one of the ingredients on morphology of the liver and the weight of experimental animals. Fodder containing soil dust was given to animals for various periods of time, the longest was 18 weeks. The results indicate that the intensity of morphological changes in the liver depends on the time of giving fodder. The observed changes referred to the vacuolization of hepatocytes, increase in the number of stellate cells Browicz-Kupffer, and apart from that lymphocytic infiltrations occurred locally. The weight of the experimental animals in the initial period (up to 3 weeks) decreased and then came back to normal in the later period, but in some animals it even decreased.

[Statistical analysis of body and lung mass of animals subjected to a single experimental insufflation of soil dust and electro-energetic ashes]. / Analiza statystyczna mas ciala i pluc zwierzat poddanych jednorazowej doswiadczalnej insuflacji pylów glebowych i popiolów elektroenergetycznych.

The studies were performed on 44 white female Wistar rats which were intratracheally administered the suspension of the soil dust and the electro-energetic ashes. The electro-energetic ashes were collected from 6 different local heat and power generating plants while the soil dust from several random places of our country. The statistical analysis of the body and the lung mass of the animals subjected to the single dust and ash insufflation was performed. The applied variants proved the statistically significant differences between the body and the lung mass. The observed differences are connected with the kinds of dust and ash used in the experiment.

[Cytoenzymatic studies on the influence of soil dust on the cornea and conjunctiva of experimental animals]. / Badania cytoenzymatyczne nad wplywem pylów glebowych na rogówke i spojówke oka zwierzat doswiadczalnych.

The studies of the effect of the soil dust on the cornea and the conjunctiva of the experimental animals have been performed. The rats were divided into 3 experimental groups and 1 control group. The animals were given 3 different, selected at random, kinds of soil dust to the conjunctival sac 3 times daily for 7 days. The results show that the soil dust has the negative effect on the organ of sight. The cornea is particularly sensitive to the action of dust, which evidenced itself by the significant changes in the histochemical reactions.

[Histological studies and clinical observations of the effect of various types of dust on the organ of vision of small laboratory animals]. / Bodania histologiczne i obserwacje kliniczne wplywu róznych pylów na narzad wzroku malych zwierzat laboratoryjnych.

For seven days, three times a day, female rats were instilled into their conjunctival sacs suspensions of power plant dusts, soil and clay. Clinically observed were inflammatory changes of the conjunctiva and cyclids margins as well as traumatisation of the corneal and conjunctival epithelia, appearing at different stages on the experiment. Cytoenzymatic examinations indicated disturbances of oxidation and reduction processes, changes in dialysis through physiologic membranes and dissociation of protein synthesis.

[Determining acid phosphatase (AcP[E.C. 3.1.3.2.]) and adenosine triphosphatase (ATPase [E.C. 3.6.1.3.]) activity in the lungs of rats following a single administration of ashes from industrial high-heat facilities]. / Aktivitätsbeurteilung von saurer Phosphatase (AcP[E.C. 3.1.3.2.]) und Adenosintriphosphatase (ATP-ase [E.C. 3.6.1.3.]) in Lungen von Ratten nach einmaliger Applikation von Aschen aus Kraftwerk-Grossfeueranlagen.

There was examined pulmonary tissue of white rats, which had been administered intratrachealy a single dose of the respirable fraction of ashes sample from 6 different power stations elektrohasting plants and hasting plants in Poland (0.2 ml suspension; 50 mg of the examined sample in 0.6 cm3 of NaCl solution). 9 months after the application of the ashes, biopsies of the left lung were taken and there was determined the activity of acid phosphatase (AcP) and adenosinetriphosphatase (ATP-ase) histoenzymatically. There was found sensitivity of these hydrolases and changes of their activity connected with chemical composition of the examined ashes.

[Histochemical studies of rat lungs after the short-term influence of soil dust]. / Histochemische Untersuchungen an Rattenlungen nach kurzdauernder Wirkung von Bodenstäuben.

There was examined the biological activity of soil dusts using histochemical methods. Intratracheal administration of dusts used in this study is a common method for testing activity of industrial dusts. The used soil dusts were characterized by high content of free silicon dioxide, 3 times higher than its content in dusts from power stations released in the process of burning coal and approximately to the amount in graphite dust. Both coal dusts and graphite dusts absorbed by the lungs cause silicoanthracosis. The investigations have been shown that soil dusts caused stimulation of the mitochondrial metabolism giving an increase of the activity of succinic dehydrogenase, lactic dehydrogenase, and ATPase induced by Mg++ ions. This was additionally confirmed by an increase of NADP activity which is an enzyme binding a chain or reactions regulating the hydrocarbonic metabolism. There was also observed an increased activity of the hydrolytic enzyme acid phosphatase. High activity occurred in the epithelium of bronchi and bronchioli and focally in pulmonary parenchyma.

[Soil dust as a factor which changes the histochemical picture of the rat lung]. / Bodenstäube als Faktor, der das histochemische Bild der Rattenlunge ändert.

White Wistar female rats only once were administered intratracheally a suspension of the soil dust in the physiologic saline. The respirable fraction of the soil dust was used for preparing the suspension of the latter. The experiment was carried out in 2 groups of rats. The animals of the 1st group received the soil dust suspension which was the mixture of the soil samples from 3 different places in Poland, the animals of the 2nd group from 3 other places in Poland. The control animals were once administered the physiologic saline. 9 months after the application of dust, the specimen of the left lung were taken for histochemical examinations. The reactions to the activity of succinic and lactate dehydrogenase, of tetrazole reductase NADP, and of acid phosphatase and ATP-ase were performed. The changes in the activity of the studied respiratory enzymes and the increase of the reaction to the acid phosphatase were observed. The results reveal the disorders of the oxidation-reduction processes and the intensification of the lytic processes in the pulmonary parenchyma of the animals exposed to the action of the soil dust.

[Effect of short-term exposure to ash from electric power plants on histochemical reactions of succinate dehydrogenase and lactate dehydrogenase in the lungs of experimental animals]. / Wplyw krótkotrwalego dzialania popiolów elektroenergetycznych na odczyny histochemiczne dehydrogenazy bursztynianowej (SDH) E.C.1.3.99.1 i dehydrogenazy mleczanowej (LDH) E.C.1.1.1.27 w plucach zwierzat doswiadczalnych.

Histochemical studies of the activity of succinic dehydrogenase (SDH) and lactic dehydrogenase (LDH) in the pulmonary tissue of experimental animals which were given four coal ashes issued from different thermal-electric power stations: group VII--from the thermal-electric power station Siekierki (Warsaw), group VIII--from Wroclaw, group IX--from Leg (Cracow) and group XI--from Lódz, were carried out. In particular ash samples the content of trace elements and free silica was determined. The studies yield the following conclusions: 1. The activity of succinic dehydrogenase (SDH) and lactic dehydrogenase (LDH) was changed in the respiratory tract and pulmonary alveoli as a result of the electroenergetic ashes applied. 2. Changes in cytoenzymatic reactions of SDH and LDH vary with the type of ash applied. The greatest changes were found in group XI where the ashes contained almost doubled amount of such elements as Mn, Zn, Pb, Cu. 3. SDH exhibited a greater sensitivity to the effects of electroenergetic ashes, as compared to LDH. 4. Changes in the activity of the test SDH and LDH enzymes indicate disorders in oxydoreductory processes of cells.

[Behavior of selected oxidoreductases in histochemical and electro- phoretic studies of the rat kidney after administration of Biseptol 480 (co-trimoxazole)]. / Beobachtungen des Verhaltens ausgewählter Oxidoreductasen in histochemischen und elektrophoretischen Untersuchungen der Rattenniere nach Verabreichung von Biseptol 480 (Co-Trimoxazole).

Examinations of the rat kidney metabolism were carried out by histological (staining with haematoxylin and eosin), histochemical (response to lactate and succinic dehydrogenases activities), and electrophoretic methods (separation of isoenzymes of lactate dehydrogenase) after treating animals with the sulphonamide Biseptol 480, for 7 (consecutive) d by stomach tube. Biseptol given to the animals exceeded 10 times human therapeutical dose. It was found out that Biseptol 480, applied to the animals with healthy urinary system, does not pathologically disturb the examined stages of the metabolic processes in the rat's kidney.