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Bitemark analysis involves the examination of both patterned injuries and contextual circumstances, combining morphological and positional data. Considering the uniqueness of human dentition, bitemarks caused by teeth on skin or impressions on flexible surfaces could assist in human identification. AIMS: to investigate the available literature systematically and evaluate the scientific evidence published over the past decade concerning the potential application of bitemark analysis in forensic identification. METHODS: Two researchers meticulously searched electronic databases from January 2012 to December 2023, including Scopus, PubMed, Web of Science, and the Cochrane Library. Adhering to the PRISMA statement guidelines, this review employed appropriate medical subject headings (MeSHs) and free-text synonyms. Strict inclusion and exclusion criteria were applied during article retrieval. RESULTS: The findings yielded controversial outcomes. Approximately two-thirds of the articles concluded that bitemark analysis is useful in forensic identification, while the remaining articles did not report statistically significant outcomes and cautioned against relying solely on bitemark analysis for identification. CONCLUSIONS: The authors assert that bitemark analysis can be a reliable and complementary method for forensic identification, contingent upon the establishment and adoption of a universally accepted global protocol for data collection, processing, and interpretation. Undoubtedly, recent years have witnessed a notable increase in research focused on bitemark identification, driven by the goal of achieving quantitative, objective, reproducible, and accurate results.
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OBJECTIVE: The aim of this study is to compare the accuracy of cone beam computed tomography (CBCT) for dental model scanning to the accuracy of model scanners. METHODS: Subjects from private practice were collected and scanned according to specific selection criteria. A total of 10 STL files were produced and used as reference files. They were printed with a three-dimensional (3D) printer and then scanned with CBCT and model scanner. For trueness evaluation, all models were scanned once with both equipments. Each file derived from each scan was compared with the corresponding reference model file. For the precision measurements, the physical model from the first master reference model file was scanned 10 times with each equipment and compared with the reference STL file. A reverse engineering software was used for all 3D best-fit comparisons. RESULTS: With regard to the measurement of trueness of each method, the calculated mean root mean square (RMS) value was 0.06±0.01mm for the CBCT, and 0.15±0.02mm for the model scanner. There was a significant difference between the two methods (P<0.01). For the evaluation of precision of each scanner, the mean RMS value was 0.0056±0.001mm for the CBCT, and 0.153±0.002mm for model scanner. There was a significant difference between the two methods (P<0.01). CONCLUSIONS: Cone Beam Computed Tomography seems to be an accurate method for scanning dental models. CBCT performs better than model scanners to scan dental models in terms of trueness and precision.
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Desenho Assistido por Computador , Tomografia Computadorizada de Feixe Cônico Espiral , Humanos , Modelos Dentários , Tomografia Computadorizada de Feixe Cônico/métodos , Software , Imageamento Tridimensional , Técnica de Moldagem OdontológicaRESUMO
Gender determination is an essential element for human identification in forensic medicine, to which the maxillary sinuses may contribute as they remain intact even after severe damage to the skull and other structures. AIM: To evaluate scientific evidence published over the last decade to determine whether maxillary sinus dimensions and volume may constitute useful parameters for forensic identification and gender determination, based only on cone-beam computed tomography images (CBCT). METHODS: This review adhered to the PRISMA statement's criteria. Four databases were searched for articles published between January 2010 and April 2023. RESULTS: Initially, 1719 records were identified. After screening, there were 2475 participants in the included studies. Of the fifteen articles selected, five reported data assessing only volumetric measurements of the maxillary sinus, seven reported data calculating only linear measurements and three reported data by combining findings of both linear and volumetric measurements of the maxillary sinus. Maxillary sinus volume was significantly higher in male participants. Maxillary sinus height was the best discriminating parameter for forensic identification with an overall accuracy ranging from 70% to 80%. CONCLUSIONS: Maxillary sinus measurements revealed anatomic variability between genders, and this approach can be applied as a complementary method for human identification.
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BACKGROUND: The current review's goal is to examine, with a critical eye, the effect of various biomedical parameters on orthodontic tooth movement in an attempt to provide the reader with related mechanisms of this issue focusing on certain key points. METHODS: This critical review was conducted using the following keywords in the search strategy: "biomedical molecules", "biomarkers", "orthodontics", "orthodontic tooth movement", "acceleration", "gene therapy", and "stem cells". Cochrane Library, Medline (PubMed), and Scopus were the databases that were used for the electronic search. Studies published until June 2023 were considered. RESULTS: The use of biomedical approaches in orthodontic tooth movement has been investigated via different procedures and applications. Surgical approaches, biomarkers affecting orthodontic tooth movement, different biological events and mechanisms, RANK, RANK-L, OPG molecular triad, and vibration methods are the basic parameters of biomedical interventions that are examined in the present review. CONCLUSIONS: The biomedical approach seems to offer a variety of applications to control orthodontic tooth movement. The scarcity of human studies, as well as the high cost and complexity of these methods, currently limit the available accurate data concerning this issue.
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Since early detection of osteoporosis is essential, the development of an efficient and cost-effective screening model would be incredibly beneficial. The aim of this study was to evaluate the diagnostic accuracy of MCW and MCI indices from dental panoramic radiographs in combination with a new variable, age at menarche, for the detection of osteoporosis. The study enrolled 150 Caucasian women (aged 45 to 86) who met the eligibility criteria, had DXA scans of the left hip and lumbar spine (L2 to L4), and were classified as osteoporotic, osteopenic, or normal based on T-score. Two observers evaluated MCW and MCI indexes on panoramic radiographs. There was a statistically significant correlation between the T-score and MCI and MCW. In addition, age at menarche had a statistically significant correlation with T-score (p = 0.006). In conclusion, in the current study, MCW proved to be more effective in detecting osteoporosis when combined with age at menarche. Individuals with MCW less than 3.0 mm and age at menarche later than 14 years old should be referred for DXA since they present high risk of osteoporosis.
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Human identification is considered as an important step in the reconstruction of biological profiles, especially of unknown individuals. Frontal sinuses (FS) have been regarded as an ideal anatomical structure for individualisation because few pathological conditions can potentially alter their shape. AIM: The aim of this review was to evaluate scientific evidence published since January 2010 and determine whether the dimensions and volume of FS might be useful parameters for gender determination and human identification, based only on cone beam computed tomography images (CBCT). METHODS: This review was performed in accordance with the PRISMA statement. Four databases were searched for articles published between January 2010 and June 2022. RESULTS: A total of 195 records were initially identified, and 90 remained after a manual duplicate check. Eight articles were selected for a full review according to the inclusion and exclusion criteria after title and abstract screening. A total of 718 participants (359 males and 359 females) were identified from the included studies. Frontal sinus volume (FSV) was significantly higher in male individuals. Frontal sinus height (FSH) and volume were the superior discriminating parameters for forensic identification. CONCLUSIONS: This review demonstrates that assessment of FS based on CBCT images could be beneficial for gender identification in forensic science. According to the obtained studies, frontal sinus volume (FSV) and frontal sinus height (FSH) are significant greater in males than in females, providing an additional complementary method. Larger sample size and common measurement protocols are needed to verify their usefulness.
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BACKGROUND: Biotechnology shows a promising future in bridging the gap between biomedical basic sciences and clinical craniofacial practice. The purpose of the present review is to investigate the applications of biotechnology in the craniofacial complex. METHODS: This critical review was conducted by using the following keywords in the search strategy: "biotechnology", "bioengineering", "craniofacial", "stem cells", "scaffolds", "biomarkers", and "tissue regeneration". The databases used for the electronic search were the Cochrane Library, Medline (PubMed), and Scopus. The search was conducted for studies published before June 2022. RESULTS: The applications of biotechnology are numerous and provide clinicians with the great benefit of understanding the etiology of dentofacial deformities, as well as treating the defected areas. Research has been focused on craniofacial tissue regeneration with the use of stem cells and scaffolds, as well as in bioinformatics with the investigation of growth factors and biomarkers capable of providing evidence for craniofacial growth and development. This review presents the biotechnological opportunities in the fields related to the craniofacial complex and attempts to answer a series of questions that may be of interest to the reader. CONCLUSIONS: Biotechnology seems to offer a bright future ahead, improving and modernizing the clinical management of cranio-dento-facial diseases. Extensive research is needed as human studies on this subject are few and have controversial results.
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OBJECTIVES: The aim of this study was to investigate the effect of the application of orthodontic tooth forces on the alveolar bone distal to the loaded teeth, in ovariectomized female rats. METHODS: Twenty-four eight-month-old Wistar rats were divided into one group ovariectomized at the age of six months and one control. An orthodontic appliance delivering a mesial traction force of 60 gr* was placed on the right maxillary 1st molar of all animals for 14 days. Histology of the alveolar bone, of the adjacent and distal teeth to the loaded molar and the contralateral side, was performed following euthanasia. RESULTS: In the non-ovariectomized rats, extensive resorption was noticed in the direction of the orthodontic movement in the 2nd and 3rd molar interdental space, whereas the respective contralateral interdental space did not show any remodeling activity. Ovariectomized rats displayed reduced osseous tissue in the interdental space of both sides. The alveolar bone in the interradicular area of the 2nd loaded molar revealed frontal resorption, whereas, the alveolar interradicular bone of the contralateral 2nd molar showed internal resorption. CONCLUSIONS: In conclusion, orthodontic forces applied to the dentoalveolar complex of ovariectomized rats affect bone remodeling, even in areas distal to the site of force application. This finding should be taken into account during orthodontic treatment of women during menopause.
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Processo Alveolar , Técnicas de Movimentação Dentária , Processo Alveolar/patologia , Animais , Remodelação Óssea/fisiologia , Feminino , Humanos , Dente Molar , Ratos , Ratos WistarRESUMO
OBJECTIVES: To investigate in growing rats the effect of intrauterine growth restriction (IUGR) on the bone mineral density of the mandible and tibia, as well as the quality of the mandibular and condylar bone. METHODS: Twelve male rats were born IUGR by mothers sustaining 50% food restriction during pregnancy. Twelve control male rats were born by mothers fed ad libitum. Dual-energy X-ray absorptiometry (DEXA) of the tibia, proximal tibial metaphysis and the mandible, biochemical markers, histology and histomorphometrical analysis on the mandibular and subchondral bone of the condyle were performed. RESULTS: IUGR significantly affected bone mineral density (BMD) of both tibial and mandibular bones. IUGR rats had significantly lower osteocalcin values (p=0.021) and phosphorus (p=0.028), but not 25-OH vitamin D (p=0.352). Bone area percentage in the mandible was significantly lower (51.21±5.54) in IUGR compared to controls (66.00±15.49), and for subchondral bone of the condyle for IUGR (47.01±6.82) compared to controls (68.27±13.37). IUGR had a significant reduction in the fibrous layer, but not the proliferating layer, with the hypertrophic layer significantly increased. CONCLUSION: Maternal restricted nutrition during gestation can affect BMD of the mandible and the tibia of the offspring animals.