Nematophagous fungi in fresh feces of cattle in the Mata region of Minas Gerais state, Brazil.

The capacity to survive gut passage is one of the desirable characteristics for nematophagous fungi to be considered potential biological control agents of gastrointestinal nematodes of livestock. From April 1995 to April 1996, a pool of 10 cow fecal samples and 10 individual samples of feces from heifers, which were raised under partial (cows) or total (heifers) confinement in the Mata Region of Minas Gerais State, Brazil, were examined monthly for the presence of nematophagous fungi. A total of 10 isolates was found in the survey. Eight isolates were recovered from the pooled samples of cow feces and two from the individual samples of heifers. Fungi were present in the cow feces during the dry months of August (two isolates of Arthrobotrys oligospora and one Monacrosporium eudermatum) and September (one isolate of Harposporium lilliputanum and one of M. gephyropagum). Fungi were also recovered at the beginning and middle of the rainy season: one isolate of A. musiformis in October, and one isolate of M. gampsosporum and one unidentified fungus which produced septate hyphae and adhesive buds in December. In the individual samples collected from heifers, fungi were present only in the months of September (end of dry season) and March (end of rainy season). One isolate each of H. lilliputanum and A. oligospora were found, respectively. Additional studies to further characterize these isolates should be encouraged.

Reduction of Haemonchus contortus infective larvae by Harposporium anguillulae in sheep faecal cultures.

Harposporium anguillulae is a common nematophagous fungus which colonizes cattle pats deposited in Brachiaria decumbens pasture in Brazil. To assess its possible effect on free living stages of trichostrongylid nematodes, the reduction of Haemonchus contortus infective stages in sheep faecal culture was evaluated in vitro. Addition of 300,000 conidia per gram of faeces caused a reduction of 99.5% compared to the control group. This result indicates that H. anguillulae could be a candidate for the development of strategies for the biological control of trichostrongylid nematodes.