RESUMO
This study aimed to evaluate the adenine nucleotides and nucleoside concentration in serum and cerebral cortex of rats infected with Trypanosma evansi. Each rat was intraperitoneally infected with 1 × 10(6) trypomastigotes suspended in cryopreserved blood (Group A; n = 18). Twelve animals were used as controls (Group B). The infected animals were monitored daily by blood smears. At days 4 and 20 post-infection (PI) it was collected serum and cerebral cortex to measure the levels of ATP, ADP, AMP and adenosine by high performance liquid chromatography (HPLC). In serum there was a significant (P < 0.05) increase in the ATP, AMP and adenosine concentrations at days 4 and 20 PI in infected rats when compared to not-infected. Furthermore, in the cerebral cortex it was observed a significant (P < 0.05) increase in the concentrations of ATP, AMP and decreased adenosine levels at day 4 PI. At day 20 PI it was only observed an increase in the AMP and adenosine concentrations in cerebral cortex of infected rats when compared to not-infected. It was not observed any difference in ADP concentration in serum and brain at days 4 and 20 PI. No change was observed histologically in the cerebral cortex of infected animals. The results allow us to conclude that infection with T. evansi in rats causes an increase in the concentrations of ATP, AMP and adenosine in serum and cerebral cortex the time periods evaluated. These alterations occurred as a result of T. evansi infection which involves neurotransmission, neuromodulation and immune response impairment confirm the importance of the purinergic system in this pathology.
Assuntos
Nucleotídeos de Adenina/sangue , Córtex Cerebral/química , Nucleosídeos/sangue , Trypanosoma/fisiologia , Tripanossomíase Africana/metabolismo , 5'-Nucleotidase/metabolismo , Nucleotídeos de Adenina/análise , Adenosina Desaminase/metabolismo , Animais , Córtex Cerebral/enzimologia , Córtex Cerebral/patologia , Cromatografia Líquida de Alta Pressão , Cães , Masculino , Nucleosídeos/análise , Parasitemia/metabolismo , Parasitemia/parasitologia , Pirofosfatases/metabolismo , Ratos , Ratos Wistar , Tripanossomíase Africana/sangue , Tripanossomíase Africana/parasitologiaRESUMO
Sera were collected from 300 domiciled cats from the municipality of Lages, Southern Brazil, to determine the prevalence of Toxoplasma gondii antibodies and risk factors associated. Tests for T. gondii antibodies were performed using indirect immunofluorescent antibody test (IFAT). Positive reactions with titers ≥1:64 were found in 43 (14.33%) cats. A significant number of seropositive cats were ≥6 month old (p = 0.03758) and had access to the streets or/and rural areas (p = 0.04185). The results indicate that T. gondii is widespread in cats in Lages with a prevalence of 14.33%.
Amostras de sangue foram obtidas de 300 gatos domiciliados para determinar a prevalência de anticorpos contra Toxoplasma gondii e possíveis fatores de risco. A detecção de anticorpos da classe IgG foi realizada por meio da Reação de Imunofluorescência Indireta (RIFI). Reações positivas com títulos ≥1:64 foram encontradas em 43 (14,33%) gatos. Os títulos encontrados variaram de 1:64 a 1:4096, sendo que 19 animais (44,19%) tiveram titulação de 1:64, 18 animais (41,86%) com 1:256, cinco animais (11,63%) com 1:1024 e um animal (2,32%) teve titulação de 1:4096. Foi verificada correlação entre soropositividade dos felinos ao T. gondii e idade (p = 0,03758) e com o acesso à rua e/ou zona rural (p = 0,04185). Os resultados indicam que T. gondii está disseminado entre os gatos domiciliados de Lages, Estado de Santa Catarina, Brasil, com uma prevalência de 14,33%.
