Impact of Wearing and Washing/Drying of Permethrin-Treated Clothing on Their Contact Irritancy and Toxicity for Nymphal Ixodes scapularis (Acari: Ixodidae) Ticks.

Permethrin-treated clothing is available as consumer products to prevent bites by tick and insect pests. We used bioassays to examine the impact of wearing and washing/drying of permethrin-treated shirts, pants, and socks, and wearing of treated shoes, on their contact irritancy and toxicity for nymphal Ixodes scapularis Say (Acari: Ixodidae) ticks, the primary vectors in the eastern United States of the causative agents of Lyme disease, human anaplasmosis, and human babesiosis. Pristine permethrin-treated clothing displayed strong contact irritancy and toxicity toward I. scapularis nymphs, with 0-30% of ticks across clothing types and tick sources displaying normal movement 1 h after forced contact for 30-120 s with treated textile. Following 16 d of wear and 16 rounds of machine washing and drying, we recorded reduced concentrations (by 50-90%) of permethrin, compared with pristine treated clothing, from shirts, pants, and socks. This loss of permethrin was associated with reduced contact irritancy and toxicity for ticks after forced contact with worn and washed/dried treated clothing: 31-67% of ticks displayed normal movement 1 h after contact. Nevertheless, the worn and washed/dried treated clothing was still superior to nontreated textile, for which 90-100% of ticks displayed normal movement. Treated shoes, which were worn but not washed, remained as toxic to the ticks as pristine treated shoes. We caution that these laboratory bioassay results should not be interpreted as being directly indicative of the outcome of using washed/worn permethrin-treated clothing in daily life. Although wear and washing/drying did reduce the irritancy and toxicity of permethrin-treated clothing for I. scapularis nymphs more than we had expected, the remaining effect might still reduce the risk of tick bites in a real-life scenario.

A SURVEY OF THE MOSQUITOES OF KOSRAE STATE, FEDERATED STATES OF MICRONESIA, 2016.

In response to an outbreak of Zika virus that started in February 2016 on Kosrae Island, Kosrae State, Federated States of Micronesia, we conducted entomological investigations, including a survey to characterize the mosquito fauna on Kosrae, from November 29 to December 8, 2016. Mosquitoes were collected using several surveillance methods in order to sample all stages of the mosquito life cycle. Eggs were collected using ovicups, larvae and pupae were sampled using standard dippers, and adults were collected using aspirators and Biogents-2 Sentinel traps. All species previously recorded from Kosrae State were found in the current survey, confirming their continued presence on the island. Aedes aegypti was detected on Lelu Island, representing a new municipal record. The collection of Ae. vexans nocturnus represents a new species record for Kosrae, increasing the number of known taxa on this island from 6 to 7. The report herein provides updated knowledge of the mosquitoes that occur on Kosrae State, Federated States of Micronesia.

New State Records of Mosquitoes for Colorado.

The 1967 treatment of the Mosquitoes of Colorado by Harmston and Lawson and subsequent publications have recorded 46 culicid species from Colorado. As part of a study to create an updated synopsis of the mosquitoes of Colorado, adult trapping at numerous localities was conducted in Colorado during the summers of 2013 and 2014. This review also included an examination of mosquito specimens in various relevant museum collections. Aedes (Ochlerotatus) niphadopsis and Ae. (Och.) spencerii spencerii were collected during the 2013 and 2014 field seasons. Records for Ae. (Och.) canadensis canadensis, Ae. (Stegomyia) aegypti, and Uranotaenia (Pseudoficalbia) anhydor syntheta were obtained from examination of museum specimens. These species constitute new state records for Colorado, with 51 species now known from the state.