Stromal vascular fraction improves the durability of autologous fat temple augmentation-A split-face randomized study using ultrasound biomicroscopy.

BACKGROUND: Autologous lipotransfer aims to restore aging-associated volume loss, but with low predictability owing to 20-90% first-year loss of transferred fat. Enrichment by adipose-derived stem cells within the stromal vascular fraction (SVF) aims to improve volume retention through their differentiation potential and paracrine actions exerted by secreted trophic and angiogenic factors. Assessing studies lacked split-face designs, and used multitudes of enrichment ratios, preparation techniques and evaluation methods ending in contradictory reports regarding enrichment advantage. AIM: To test whether enriching the autologous fat graft with SVF will increase its residual volume as compared to non-enriched graft. A standardized enrichment protocol and ratio and objective assessment were employed. PATIENTS AND METHODS: In a split-face design, and after random assignment, bilateral temple augmentation using non-enriched versus SVF-enriched autologous lipotransfer were compared in middle-aged females otherwise healthy non-pregnant or breast-feeding females abstaining from esthetic or weight-controlling procedures. Temple volume scale (TVS), skin layers' thickness measured by ultrasound biomicroscopy (UBM), visual analog scale for patients' satisfaction, and side effects were blindly assessed at 1 week, 3 months, and 6 months. RESULTS: In the included 15 females, TVS was significantly lower (0.5 ± 0.5 versus 1.1 ± 0.7, P = 0.0001), and% hypodermal augmentation was significantly higher (70.92 ± 58.09 versus 18.93 ± 19.33, P = 0.001) on the SVF-enriched side at 6 months. Patient satisfaction was similar bilaterally (P = 1), as were sequelae frequencies as lumping, edema, and ecchymosis. CONCLUSION: SVF enrichment of transferred fat significantly improved its residual volume at 6 months; a conclusion that needs further validation. UBM was an informative objective tool for the following temple skin thickness changes. Trial registration clinical trials.gov (NCT03965936).

Glutathione S-transferase Mu-1 gene polymorphism in Egyptian patients with idiopathic male infertility.

The aim of this study was to examine whether an association exists between glutathione S-transferase Mu-1 (GSTM1) gene polymorphism and idiopathic male infertility. Sixty men with primary idiopathic infertility and 60 fertile men, serving as controls, were recruited for the study. The polymorphism was analysed using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique. The frequency of GSTM1 null genotype was observed to be higher in infertile men 40% in comparison with 33.3% in the fertile men, but this difference was not statistically significant. There was statistically significant difference between cases and controls as regards GSTM1 genotype distribution ((MC) P = 0.006*) in GSTM1-positive men. Patients with the GSTM1 null genotype had significantly lower sperm concentrations and total sperm count when compared with patients with GSTM1-positive genotype. In the control group, men with GSTM1 null genotype had significantly lower sperm concentrations but not total sperm count when compared with men with GSTM1-positive genotype. The results of this study suggest a possible negative effect of GSTM1 null genotype on the spermatogenic potential of the testis.

Risk profiles for sexually transmitted diseases among patients attending the venereal disease clinic at Alexandria Main University Hospital.

Risk factors for sexually transmitted diseases (STDs) were assessed among 54 male and 36 female patients attending a venereal disease clinic. Sociodemographic data and information on sexual behaviour/STD history were collected. Patients were examined and specimens taken for laboratory diagnosis. Multivariate logistic regression analysis revealed that the significant predictors of genital infections among the male patients were: being unmarried, having multiple sexual partners, exposure to a symptomatic sexual partner, high frequency of intercourse per week, having repeated episode(s) of STDs and practising coitus interfemoris. In the female patients, exposure to a symptomatic sexual partner and high frequency of intercourse per week were the only significant predictors.

Risk profiles for sexually transmitted diseases among patients attending the venereal disease clinic at Alexandria Main University Hospital

Risk factors for sexually transmitted diseases [STDs] were assessed among 54 male and 36 female patients attending a venereal disease clinic. Sociodemographic data and information on sexual behaviour/STD history were collected. Patients were examined and specimens taken for laboratory diagnosis. Multivariate logistic regression analysis revealed that the significant predictors of genital infections among the male patients were: being unmarried, having multiple sexual partners, exposure to a symptomatic sexual partner, high frequency of intercourse per week, having repeated episode[s] of STDs and practising coitus interfemoris. In the female patients, exposure to a symptomatic sexual partner and high frequency of intercourse per week were the only significant predictors