Advanced characterization of natural biofilm on nanofiber scaffold.

Nanofiber scaffolds provide numerous advantages over common carriers engineered for microorganisms. The most important advantage is an increased speed of primary surface colonization (up to four times faster), which shortens the time required for the areal biofilm formation and optimum performance of attached microorganisms (higher efficiency of biological activity of up to twice as fast). Image analysis predicts early formation of biofilm even in beginning stages; analysis of biofilm reveals the different structures of bacterial colonies on both scaffolds (higher porosity, size, and number of bacterial colonies on nanofiber's surface). The image analysis correlates well with determinations of dry matter (linear correlation of 0.96) and proteins (linear correlation of 0.89).

[Aggressive papillary adenoma of a digit]. / Agresivní digitální papilární adenom.

A case of a rare skin adnexal tumour, aggressive digital papillary adenoma was described. The tumour had a characteristic forefinger localization, showed microscopical cystopapillary pattern combined with more solid and partly cribriform structures. Tumour cells were represented by lighter cuboid and darker smaller spindle elements. In immunohistology, the tumour cells were positive for epithelial markers (CK, EMA), minority of them showed S 100 protein positivity. Basal cells along the collagenous stroma tissue were strongly actin positive. The light microscopical picture was complemented by electron microscopy for the sake of a more detailed description of tumour cells.