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1.
Sports Biomech ; 21(10): 1176-1188, 2022 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-32308138

RESUMO

The aim of this acute cross-sectional study was to quantify the kinematic and kinetic changes that occur during sprint acceleration when lower body WR is worn. Fifteen male rugby athletes (19 years; 181 cm; 91 kg) were assessed during maximal effort over-ground and treadmill sprinting over 20 m under three different loading conditions: 0%, 3% and 5% body mass (BM) added weight attached to the lower body. Treadmill data provided a convenient estimate of kinetic changes in the absence of in-ground force plates. The loaded conditions resulted in significantly increased ground contact time (5 to 6%) and decreased step frequency (-2 to -3%) during sprint accelerations (effect size = 0.32-0.72). Moderate WR loading (3% BM) resulted in increased (9%; effect size = 0.66) theoretical maximum horizontal force (relative to BM) and unchanged 20 m sprint times (p > 0.05). Heavier WR loading (5% BM) resulted in a significant decrease (-4%) in vertical ground reaction forces (relative to total system mass) and slower (1 to 2%) 20 m sprint times (effect size = 0.38-0.70). Lower body WR loading up to 5% BM can provide specific sprint training overload, while affecting sprint acceleration biomechanics by ≤ 6%.


Assuntos
Desempenho Atlético , Corrida , Dispositivos Eletrônicos Vestíveis , Aceleração , Fenômenos Biomecânicos , Estudos Transversais , Humanos , Masculino
2.
Sports Med Open ; 7(1): 48, 2021 Jul 15.
Artigo em Inglês | MEDLINE | ID: mdl-34268627

RESUMO

Maximal muscular power production is of fundamental importance to human functional capacity and feats of performance. Here, we present a synthesis of literature pertaining to physiological systems that limit maximal muscular power during cyclic actions characteristic of locomotor behaviours, and how they adapt to training. Maximal, cyclic muscular power is known to be the main determinant of sprint cycling performance, and therefore we present this synthesis in the context of sprint cycling. Cyclical power is interactively constrained by force-velocity properties (i.e. maximum force and maximum shortening velocity), activation-relaxation kinetics and muscle coordination across the continuum of cycle frequencies, with the relative influence of each factor being frequency dependent. Muscle cross-sectional area and fibre composition appear to be the most prominent properties influencing maximal muscular power and the power-frequency relationship. Due to the role of muscle fibre composition in determining maximum shortening velocity and activation-relaxation kinetics, it remains unclear how improvable these properties are with training. Increases in maximal muscular power may therefore arise primarily from improvements in maximum force production and neuromuscular coordination via appropriate training. Because maximal efforts may need to be sustained for ~15-60 s within sprint cycling competition, the ability to attenuate fatigue-related power loss is also critical to performance. Within this context, the fatigued state is characterised by impairments in force-velocity properties and activation-relaxation kinetics. A suppression and leftward shift of the power-frequency relationship is subsequently observed. It is not clear if rates of power loss can be improved with training, even in the presence adaptations associated with fatigue-resistance. Increasing maximum power may be most efficacious for improving sustained power during brief maximal efforts, although the inclusion of sprint interval training likely remains beneficial. Therefore, evidence from sprint cycling indicates that brief maximal muscular power production under cyclical conditions can be readily improved via appropriate training, with direct implications for sprint cycling as well as other athletic and health-related pursuits.

3.
J Sports Sci ; 38(1): 29-37, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31631783

RESUMO

This study investigated the role of reactive and eccentric strength in stiffness regulation during maximum velocity sprinting (Vmax) in team sport athletes compared with highly trained sprinters. Thirteen team sport athletes and eleven highly trained sprinters were recruited. Vmax was measured using radar, and stiffness regulation was inferred from modelled vertical and leg spring stiffness. Reactive strength (RSI) was determined from a 0.50 m drop jump, and an eccentric back squat was used to assess maximum isoinertial eccentric force. Trained sprinters attained a higher Vmax than team sport athletes, partly due to a briefer contact time and higher vertical stiffness. Trained sprinters exhibited a moderately higher RSI via the attainment of a briefer and more forceful ground contact phase, while RSI also demonstrated large to very large associations with vertical stiffness and Vmax, respectively. Isoinertial eccentric force was largely correlated with Vmax, but only moderately correlated with vertical stiffness. Reactive and eccentric strength contribute to the ability to regulate leg spring stiffness at Vmax, and subsequently, the attainment of faster sprinting speeds in highly trained sprinters versus team sport athletes. However, stiffness regulation appears to be a task-specific neuromuscular skill, reinforcing the importance of specificity in the development of sprint performance.


Assuntos
Desempenho Atlético/fisiologia , Extremidade Inferior/fisiologia , Força Muscular/fisiologia , Músculo Esquelético/fisiologia , Condicionamento Físico Humano , Corrida/fisiologia , Aceleração , Adulto , Fenômenos Biomecânicos , Estudos Transversais , Feminino , Humanos , Masculino , Adulto Jovem
4.
Int J Sports Physiol Perform ; 14(8): 1147-1150, 2019 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-30702359

RESUMO

PURPOSE: Anaerobic speed reserve (ASR), defined as the speed range from velocity associated with maximal oxygen uptake (vVO2max) to maximal sprint speed, has recently been shown to be an important tool for middle-distance coaches to meet event surge demands and inform on the complexity of athlete profiles. To enable field application of ASR, the relationship between gun-to-tape 1500-m average speed (1500v) and the vVO2max for the determination of lower landmark of the ASR was assessed in elite middle-distance runners. METHODS: A total of 8 national and 4 international middle-distance runners completed a laboratory-measured vVO2max assessment within 6 wk of a nonchampionship 1500-m gun-to-tape race. ASR was calculated using both laboratory-derived vVO2max (ASR-LAB) and 1500v (ASR-1500v), with maximal sprint speed measured using radar technology. RESULTS: 1500v was on average +2.06 ± 1.03 km/h faster than vVO2max (moderate effect, very likely). ASR-LAB and ASR-1500v mean differences were -2.1 ± 1.5 km/h (large effect, very likely). 1500v showed an extremely large relationship with vVO2max, r = .90 ± .12 (most likely). Using this relationship, a linear-regression vVO2max-estimation equation was derived as vVO2max (km/h) = (1500v [km/h] - 14.921)/0.4266. CONCLUSIONS: A moderate difference was evident between 1500v and vVO2max in elite middle-distance runners. The present regression equation should be applied for an accurate field prediction of vVO2max from 1500-m gun-to-tape races. These findings have strong practical implications for coaches lacking access to a sports physiology laboratory who seek to monitor and profile middle-distance runners.


Assuntos
Desempenho Atlético/fisiologia , Consumo de Oxigênio , Corrida/fisiologia , Anaerobiose , Atletas , Teste de Esforço , Humanos , Valor Preditivo dos Testes
5.
Sports Biomech ; 18(1): 88-99, 2019 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-29125040

RESUMO

Radar technology can be used to perform horizontal force-velocity-power profiling during sprint-running. The aim of this study was to determine the reliability of radar-derived profiling results from short sprint accelerations. Twenty-seven participants completed three 30 m sprints (intra-day analysis), and nine participants completed the testing session on four separate days (inter-day analysis). The majority of radar-derived kinematic and kinetic descriptors of short sprint performance had acceptable intra-day and inter-day reliability [intraclass correlation coefficient (ICC) ≥ 0.75 and coefficient of variation (CV) ≤ 10%], but split times over the initial 10 m and some variables that include a horizontal force component had only moderate relative reliability (ICC = 0.49-0.74). Comparing the average of two sprint trials between days resulted in acceptable reliability for all variables except the relative slope of the force-velocity relationship (S Fvrel; ICC = 0.74). Practitioners should average sprint test results over at least two trials to reduce measurement variability, particularly for outcome variables with a horizontal force component and for sprint distances of less than 10 m from the start.


Assuntos
Aceleração , Radar , Corrida/fisiologia , Adolescente , Fenômenos Biomecânicos , Humanos , Masculino , Fenômenos Mecânicos , Reprodutibilidade dos Testes
6.
Int J Sports Physiol Perform ; 14(4): 501-508, 2019 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-30300023

RESUMO

PURPOSE: In recent years (2011-2016), men's 800-m championship running performances have required greater speed than previous eras (2000-2009). The "anaerobic speed reserve" (ASR) may be a key differentiator of this performance, but profiles of elite 800-m runners and their relationship to performance time have yet to be determined. METHODS: The ASR-determined as the difference between maximal sprint speed (MSS) and predicted maximal aerobic speed (MAS)-of 19 elite 800- and 1500-m runners was assessed using 50-m sprint and 1500-m race performance times. Profiles of 3 athlete subgroups were examined using cluster analysis and the speed reserve ratio (SRR), defined as MSS/MAS. RESULTS: For the same MAS, MSS and ASR showed very large negative (both r = -.74 ± .30, ±90% confidence limits; very likely) relationships with 800-m performance time. In contrast, for the same MSS, ASR and MAS had small negative relationships (both r = -.16 ± .54; possibly) with 800-m performance. ASR, 800-m personal best, and SRR best defined the 3 subgroups along a continuum of 800-m runners, with SRR values as follows: 400-800 m ≥ 1.58, 800 m ≤ 1.57 to ≥ 1.48, and 800-1500 m ≤ 1.47 to ≥ 1.36. CONCLUSION: MSS had the strongest relationship with 800-m performance, whereby for the same MSS, MAS and ASR showed only small relationships to differences in 800-m time. Furthermore, the findings support the coaching observation of three 800-m subgroups, with the SRR potentially representing a useful and practical tool for identifying an athlete's 800-m profile. Future investigations should consider the SRR framework and its application for individualized training approaches in this event.


Assuntos
Desempenho Atlético/fisiologia , Comportamento Competitivo/fisiologia , Consumo de Oxigênio , Corrida/fisiologia , Humanos , Masculino
7.
Sports Med ; 49(6): 843-852, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-30374943

RESUMO

Recent evidence indicates that the modern-day men's 800 m runner requires a speed capability beyond that of previous eras. In addition, the appreciation of different athlete subgroups (400-800, 800, 800-1500 m) implies a complex interplay between the mechanical (aerial or terrestrial) and physiological characteristics that enable success in any individual runner. Historically, coach education for middle-distance running often emphasises aerobic metabolic conditioning, while it relatively lacks consideration for an important neuromuscular and mechanical component. Consequently, many 800 m runners today may lack the mechanical competence needed to achieve the relaxed race pace speed required for success, resulting in limited ability to cope with surges, run faster first laps or close fast. Mechanical competence may refer to the skilled coordination of neuromuscular/mechanical (stride length/frequency/impulse) and metabolic components needed to sustain middle-distance race pace and adjust to surges efficiently. The anaerobic speed reserve (ASR) construct (difference between an athlete's velocity at maximal oxygen uptake [v[Formula: see text]O2max]-the first speed at which maximal oxygen uptake [[Formula: see text]O2max] is attained) and their maximal sprint speed (MSS) offers a framework to assess a runner's speed range relative to modern-day race demands. While the smooth and relaxed technique observed in middle-distance runners is often considered causal to running economy measured during submaximal running, little empirical evidence supports such an assumption. Thus, a multidisciplinary approach is needed to examine the underpinning factors enabling elite 800 m running race pace efficiency. Here, we argue for the importance of utilising the ASR and MSS measurement to ensure middle-distance runners have the skills to compete in the race-defining surges of modern-day 800 m running.


Assuntos
Desempenho Atlético/fisiologia , Corrida/fisiologia , Anaerobiose , Atletas , Desempenho Atlético/tendências , Humanos , Masculino , Consumo de Oxigênio , Corrida/tendências
8.
J Strength Cond Res ; 32(10): 2750-2761, 2018 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-30113915

RESUMO

Douglas, J, Pearson, S, Ross, A, and McGuigan, M. Effects of accentuated eccentric loading on muscle properties, strength, power, and speed in resistance-trained rugby players. J Strength Cond Res 32(10): 2750-2761, 2018-The purpose of this study was to determine the effects of slow and fast tempo resistance training incorporating accentuated eccentric loading (AEL) compared with traditional resistance training (TRT) in trained rugby players. Fourteen subjects (19.4 ± 0.8 years, 1.82 ± 0.05 m, 97.0 ± 11.6 kg, and relative back squat 1 repetition maximum [1RM]: 1.71 ± 0.24 kg·BM) completed either AEL (n = 7) or TRT (n = 7) strength and power protocols. Two 4-week phases of training were completed. The first phase emphasized a slow eccentric tempo, and the second phase emphasized a fast eccentric tempo. Back squat 1RM, inertial load peak power, drop jump reactive strength index (RSI), 40-m speed, maximum sprinting velocity (Vmax), and vastus lateralis (VL) muscle architectural variables were determined at baseline and after each phase of training. Slow AEL elicited superior improvements in back squat 1RM (+0.12 kg·BM; effect size [ES]: 0.48; and 90% confidence interval [CI]: 0.14, 0.82), 40-m time (-0.07 seconds; ES: 0.28; and CI: 0.01-0.55), and Vmax (+0.20 m·s; ES: 0.52; and CI: 0.18-0.86) vs. slow TRT. Fast AEL elicited a small increase in RSI but impaired speed. There was a likely greater increase in peak power with fast TRT (+0.72 W·kg; ES: 0.40; and CI: 0.00-0.79) vs. fast AEL alongside a small increase in VL pennation angle. The short-term incorporation of slow AEL was superior to TRT in improving strength and maximum velocity sprinting speed in rugby players undertaking a concurrent preparatory program. The second 4-week phase of fast AEL may have exceeded recovery capabilities compared with fast TRT.


Assuntos
Futebol Americano/fisiologia , Força Muscular , Músculo Esquelético/fisiologia , Treinamento Resistido/métodos , Teste de Esforço , Humanos , Masculino , Adulto Jovem
10.
Int J Sports Physiol Perform ; 13(2): 246-249, 2018 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-28488905

RESUMO

PURPOSE: To assess the longitudinal evolution of tactical behaviors used to medal in men's 800-m Olympic Games (OG) or world-championship (WC) events in the recent competition era (2000-2016). METHODS: Thirteen OG and WC events were characterized for 1st- and 2nd-lap splits using available footage from YouTube. Positive pacing strategies were defined as a faster 1st lap. Season's best 800-m time and world ranking, reflective of an athlete's "peak condition," were obtained to determine relationships between adopted tactics and physical condition prior to the championships. Seven championship events provided coverage of all medalists to enable determination of average 100-m speed and sector pacing of medalists. RESULTS: From 2011 onward, 800-m OG and WC medalists showed a faster 1st lap by 2.2 ± 1.1 s (mean, ±90% confidence limits; large difference, very likely), contrasting a possibly faster 2nd lap from 2000 to 2009 (0.5, ±0.4 s; moderate difference). A positive pacing strategy was related to a higher world ranking prior to the championships (r = .94, .84-.98; extremely large, most likely). After 2011, the fastest 100-m sector from 800-m OG and WC medalists was faster than before 2009 by 0.5, ±0.2 m/s (large difference, most likely). CONCLUSIONS: A secular change in tactical racing behavior appears evident in 800-m championships; since 2011, medalists have largely run faster 1st laps and have faster 100-m sector-speed requirements. This finding may be pertinent for training, tactical preparation, and talent identification of athletes preparing for 800-m running at OGs and WCs.


Assuntos
Desempenho Atlético/fisiologia , Comportamento Competitivo/fisiologia , Corrida/fisiologia , Desempenho Atlético/psicologia , Tomada de Decisões/fisiologia , Humanos , Masculino , Condicionamento Físico Humano , Corrida/psicologia
11.
J Strength Cond Res ; 32(6): 1562-1570, 2018 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-28930875

RESUMO

Douglas, J, Pearson, S, Ross, A, and McGuigan, M. Kinetic determinants of reactive strength in highly trained sprint athletes. J Strength Cond Res 32(6): 1562-1570, 2018-The purpose of this study was to determine the braking and propulsive phase kinetic variables underpinning reactive strength in highly trained sprint athletes in comparison with a nonsprint-trained control group. Twelve highly trained sprint athletes and 12 nonsprint-trained participants performed drop jumps (DJs) from 0.25, 0.50, and 0.75 m onto a force plate. One familiarization session was followed by an experimental testing session within the same week. Reactive strength index (RSI), contact time, flight time, and leg stiffness were determined. Kinetic variables including force, power, and impulse were assessed within the braking and propulsive phases. Sprint-trained athletes demonstrated higher RSI vs. nonsprint-trained participants across all drop heights {3.02 vs. 2.02; ES (±90% confidence limit [CL]): 3.11 ± 0.86}. This difference was primarily attained by briefer contact times (0.16 vs. 0.22 seconds; effect size [ES]: -1.49 ± 0.53) with smaller differences observed for flight time (0.50 vs. 0.46 seconds; ES: 0.53 ± 0.58). Leg stiffness, braking and propulsive phase force, and power were higher in sprint-trained athletes. Very large differences were observed in mean braking force (51 vs. 38 N·kg; ES: 2.57 ± 0.73) which was closely associated with contact time (r ±90% CL: -0.93 ± 0.05). Sprint-trained athletes exhibited superior reactive strength than nonsprint-trained participants. This was due to the ability to strike the ground with a stiffer leg spring, an enhanced expression of braking force, and possibly an increased utilization of elastic structures. The DJ kinetic analysis provides additional insight into the determinants of reactive strength which may inform subsequent testing and training.


Assuntos
Força Muscular , Músculo Esquelético/fisiologia , Condicionamento Físico Humano/fisiologia , Corrida/fisiologia , Adolescente , Adulto , Teste de Esforço , Humanos , Cinética , Masculino , Adulto Jovem
12.
Sports Med ; 47(4): 663-675, 2017 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-27638040

RESUMO

An eccentric contraction involves the active lengthening of muscle under an external load. The molecular and neural mechanisms underpinning eccentric contractions differ from those of concentric and isometric contractions and remain less understood. A number of molecular theories have been put forth to explain the unexplained observations during eccentric contractions that deviate from the predictions of the established theories of muscle contraction. Postulated mechanisms include a strain-induced modulation of actin-myosin interactions at the level of the cross-bridge, the activation of the structural protein titin, and the winding of titin on actin. Accordingly, neural strategies controlling eccentric contractions also differ with a greater, and possibly distinct, cortical activation observed despite an apparently lower activation at the level of the motor unit. The characteristics of eccentric contractions are associated with several acute physiological responses to eccentrically-emphasised exercise. Differences in neuromuscular, metabolic, hormonal and anabolic signalling responses during, and following, an eccentric exercise bout have frequently been observed in comparison to concentric exercise. Subsequently, the high levels of muscular strain with such exercise can induce muscle damage which is rarely observed with other contraction types. The net result of these eccentric contraction characteristics and responses appears to be a novel adaptive signal within the neuromuscular system.


Assuntos
Exercício Físico/fisiologia , Contração Isométrica/fisiologia , Contração Muscular/fisiologia , Humanos , Músculo Esquelético
13.
Sports Med ; 47(5): 917-941, 2017 May.
Artigo em Inglês | MEDLINE | ID: mdl-27647157

RESUMO

BACKGROUND: Resistance training is an integral component of physical preparation for athletes. A growing body of evidence indicates that eccentric strength training methods induce novel stimuli for neuromuscular adaptations. OBJECTIVE: The purpose of this systematic review was to determine the effects of eccentric training in comparison to concentric-only or traditional (i.e. constrained by concentric strength) resistance training. METHODS: Searches were performed using the electronic databases MEDLINE via EBSCO, PubMed and SPORTDiscus via EBSCO. Full journal articles investigating the long-term (≥4 weeks) effects of eccentric training in healthy (absence of injury or illness during the 4 weeks preceding the training intervention), adult (17-35 years), human participants were selected for the systematic review. A total of 40 studies conformed to these criteria. RESULTS: Eccentric training elicits greater improvements in muscle strength, although in a largely mode-specific manner. Superior enhancements in power and stretch-shortening cycle (SSC) function have also been reported. Eccentric training is at least as effective as other modalities in increasing muscle cross-sectional area (CSA), while the pattern of hypertrophy appears nuanced and increased CSA may occur longitudinally within muscle (i.e. the addition of sarcomeres in series). There appears to be a preferential increase in the size of type II muscle fibres and the potential to exert a unique effect upon fibre type transitions. Qualitative and quantitative changes in tendon tissue that may be related to the magnitude of strain imposed have also been reported with eccentric training. CONCLUSIONS: Eccentric training is a potent stimulus for enhancements in muscle mechanical function, and muscle-tendon unit (MTU) morphological and architectural adaptations. The inclusion of eccentric loads not constrained by concentric strength appears to be superior to traditional resistance training in improving variables associated with strength, power and speed performance.


Assuntos
Adaptação Fisiológica , Exercício Físico/fisiologia , Contração Muscular/fisiologia , Fibras Musculares Esqueléticas/fisiologia , Força Muscular/fisiologia , Músculo Esquelético/fisiologia , Adolescente , Adulto , Humanos , Educação Física e Treinamento , Treinamento Resistido/métodos
14.
Sports Med ; 46(11): 1619-1645, 2016 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-26914267

RESUMO

BACKGROUND: Advanced testing technologies enable insight into the kinematic and kinetic determinants of sprint acceleration performance, which is particularly important for field-based team-sport athletes. Establishing the reliability and validity of the data, particularly from the acceleration phase, is important for determining the utility of the respective technologies. OBJECTIVE: The aim of this systematic review was to explain the utility, reliability, validity and limitations of (1) radar and laser technology, and (2) non-motorised treadmill (NMT) and torque treadmill (TT) technology for providing kinematic and kinetic measures of sprint acceleration performance. DATA SOURCES: A comprehensive search of the CINAHL Plus, MEDLINE (EBSCO), PubMed, SPORTDiscus, and Web of Science databases was conducted using search terms that included radar, laser, non-motorised treadmill, torque treadmill, sprint, acceleration, kinetic, kinematic, force, and power. METHODS: Studies examining the kinematics or kinetics of short (≤10 s), maximal-effort sprint acceleration in adults or children, which included an assessment of reliability or validity of the advanced technologies of interest, were included in this systematic review. Absolute reliability, relative reliability and validity data were extracted from the selected articles and tabulated. The level of acceptance of reliability was a coefficient of variation (CV) ≤10 % and an intraclass correlation coefficient (ICC) or correlation coefficient (r) ≥0.70. RESULTS: A total of 34 studies met the inclusion criteria and were included in the qualitative analysis. Generally acceptable validity (r = 0.87-0.99; absolute bias 3-7 %), intraday reliability (CV ≤9.5 %; ICC/r ≥0.84) and interday reliability (ICC ≥0.72) were reported for data from radar and laser. However, low intraday reliability was reported for the theoretical maximum horizontal force (ICC 0.64) within adolescent athletes, and low validity was reported for velocity during the initial 5 m of a sprint acceleration (bias up to 0.41 m/s) measured with a laser device. Acceptable reliability of results from NMT and TT was only ensured when testing protocols involved sufficient familiarisation, a high sampling rate (≥200 Hz), a 'blocked' start position, and the analysis of discrete steps rather than arbitrary time periods. Sprinting times and speeds were 20-28 % slower on a TT, 28-67 % slower on an NMT, and only 9-64 % of the variance in overground measurements of speed and time (≤30 m) was explained by results from an NMT. There have been no reports to date of criterion validity of kinetic measures of sprint acceleration performance on NMT andTT, and only limited results regarding acceptable concurrent validity of radar-derived kinetic data. CONCLUSIONS: Radar, laser, NMT and TT technologies can be used to reliably measure sprint acceleration performance and to provide insight into the determinants of sprinting speed. However, further research is required to establish the validity of the kinetic measurements made with NMT and TT. Radar and laser technology may not be suitable for measuring the first few steps of a sprint acceleration.


Assuntos
Aceleração , Atletas , Desempenho Atlético/fisiologia , Teste de Esforço/instrumentação , Corrida/fisiologia , Humanos , Reprodutibilidade dos Testes
15.
Cancer Immunol Res ; 2(5): 469-79, 2014 May.
Artigo em Inglês | MEDLINE | ID: mdl-24795359

RESUMO

Induction of antitumor immunity using autologous tumor proteins is an attractive approach to cancer therapy. However, better methods and stimulants to present these autologous proteins back to the immune system are needed. Here, we identify streptavidin as a novel carrier protein and stimulant, and test the efficacy of both syngeneic (rat) and autologous vaccines (dogs) using streptavidin in combination with reduced soluble tumor proteins. Initial syngeneic vaccine studies in the 9L rat glioma model were used to optimize vaccine dose and selectivity. Cytokine and blood analysis was used to monitor the response. Rats receiving two vaccinations of syngeneic tumor vaccine demonstrated a statistically significant (P < 0.05) survival advantage compared with controls (adjuvant only). Notably, vaccination also led to remission rates of between 30% and 60% in the aggressive 9L glioma model. Antibodies to streptavidin were detected in the serum of vaccinated rats; however, antibody levels did not correlate with the response. The cytokine TNF-α was upregulated in vaccine-treated rats, whereas ICAM1 was downregulated. After engraftment, vaccinated rats maintained CD4(+), CD8(+) T cells, and total lymphocyte levels closer to normal baseline than those in the controls. Twenty-five dogs treated with autologous vaccine preparations using streptavidin as a stimulant showed no adverse reactions, irrespective of additional chemotherapy and other medications. In this study, we developed a novel method for producing syngeneic and autologous vaccines using streptavidin selectivity and immunogenicity. These vaccines show efficacy in the 9L glioma rat model. Safety was also demonstrated in canine patients presenting with cancer treated with autologous vaccine.


Assuntos
Adjuvantes Imunológicos , Vacinas Anticâncer/imunologia , Estreptavidina/imunologia , Animais , Vacinas Anticâncer/administração & dosagem , Vacinas Anticâncer/efeitos adversos , Linhagem Celular Tumoral , Citocinas/sangue , Modelos Animais de Doenças , Cães , Feminino , Glioma/imunologia , Glioma/mortalidade , Glioma/patologia , Glioma/terapia , Neoplasias/imunologia , Neoplasias/metabolismo , Neoplasias/terapia , Ratos , Estreptavidina/administração & dosagem
16.
Med Sci Sports Exerc ; 44(12): 2331-9, 2012 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-22811028

RESUMO

PURPOSE: Effects of resistance training and detraining on glucose and insulin responses to an oral glucose load, muscle fiber type, and muscular performance in the offspring of those with type 2 diabetes (familial insulin resistant (FIR)) were investigated. METHODS: Six FIR participants and 10 controls (C) completed 9 wk of resistance training and 9 wk of detraining. Measures of strength and power, an oral glucose tolerance test, and a muscle biopsy to determine myosin heavy chain (MHC) fiber composition were taken at baseline (T1), after training (T2), and after detraining (T3). RESULTS: Three-repetition maximum increased (P ≤ 0.001) similarly in both groups in all strength measures, e.g., leg press (FIR T1, T2: 121 ± 34 kg, 186 ± 50 kg; C T1, T2: 137 ± 42 kg, 206 ± 64 kg, respectively (means ± SD)). Wingate peak power increased (FIR T1, T2: 505 ± 137 W, 523 ± 143 W; C T1, T2: 636 ± 211 W, 672 ± 223 W, respectively; P ≤ 0.005 (means ± SD)). Training reduced insulin area under the curve more (P = 0.050) in FIR (T1, T2: 1219 ± 734 pmol·L, 837 ± 284 pmol·L, respectively (means ± SD)) than that in C (T1, T2: 647 ± 268 pmol·L, 635 ± 258 pmol·L, respectively (means ± SD)). MHC distribution did not change with training. Strength (three-repetition maximum measures) decreased with detraining (P ≤ 0.001) although Wingate power did not. Detraining increased insulin area under the curve (P = 0.018) in FIR (T2, T3: 837 ± 285 pmol·L, 1040 ± 194 pmol·L, respectively (means ± SD)) but not in C (T2, T3: 635 ± 258 pmol·L, 625 ± 213 pmol·L, respectively (means ± SD)). MHC IIX fibers increased with detraining (P = 0.026). CONCLUSION: FIR appears to have exaggerated responses to resistance training and detraining, with a greater reduction in insulin release with glucose ingestion after training and increase when training ceases. Resistance training has a significant effect on insulin responses and may reduce future risk of type 2 diabetes mellitus among FIR.


Assuntos
Filho de Pais com Deficiência , Diabetes Mellitus Tipo 2 , Resistência à Insulina/fisiologia , Fibras Musculares de Contração Rápida/fisiologia , Fibras Musculares de Contração Lenta/fisiologia , Treinamento Resistido , Adulto , Estudos de Casos e Controles , Teste de Esforço , Feminino , Teste de Tolerância a Glucose , Humanos , Insulina/metabolismo , Secreção de Insulina , Masculino , Fibras Musculares de Contração Rápida/citologia , Fibras Musculares de Contração Lenta/citologia , Força Muscular/fisiologia , Nova Zelândia , Treinamento Resistido/métodos , Adulto Jovem
17.
Eur J Appl Physiol ; 112(5): 1663-9, 2012 May.
Artigo em Inglês | MEDLINE | ID: mdl-21874551

RESUMO

It is perceived that long haul travel, comprising of rapid movement across several time zones is detrimental to performance in elite athletes. However, available data is equivocal on the impact of long haul travel on maximal explosive movements. The aim of this study was to quantify the impact of long haul travel on lower body muscle performance. Five elite Australian skeleton athletes (1 M, 4 F) undertook long haul flight from Australia to Canada (LH(travel)), while seven national team Canadian skeleton athletes (1 M, 6 F) acted as controls (NO(travel)). Lower body power assessments were performed once per day between 09:30 and 11:00 h local time for 11 days. Lower body power tests comprised of box drop jumps, squat jump (SJ) and countermovement jumps (CMJ). The LH(travel) significantly decreased peak and mean SJ velocity but not CMJ velocity in the days following long haul flight. CMJ height but not SJ height decreased significantly in the LH(travel) group. The peak velocity, mean velocity and jump power eccentric utilisation ratio for the LH(travel) group all significantly increased 48 h after long haul flight. Anecdotally athletes perceived themselves as 'jet-lagged' and this corresponded with disturbances observed in 'one-off' daily jumping ability between 09:30 and 11:00 h after eastward long haul travel from Australia to North America when compared to non-travel and baseline controls.


Assuntos
Desempenho Atlético/fisiologia , Síndrome do Jet Lag/fisiopatologia , Movimento/fisiologia , Músculo Esquelético/fisiologia , Esportes/fisiologia , Adulto , Aeronaves , Atletas , Austrália , Canadá , Feminino , Humanos , Extremidade Inferior , Masculino , Viagem
18.
J Sports Sci ; 27(4): 397-404, 2009 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-19191166

RESUMO

The aims of this study were to talent transfer, rapidly develop, and qualify an Australian female athlete in the skeleton event at the 2006 Torino Winter Olympic Games and quantify the volume of skeleton-specific training and competition that would enable this to be achieved. Initially, 26 athletes were recruited through a talent identification programme based on their 30-m sprint time. After attending a selection camp, 10 athletes were invited to undertake an intensified skeleton training programme. Four of these athletes were then selected to compete for Australia on the World Cup circuit. All completed runs and simulated push starts were documented over a 14-month period. The athlete who eventually represented Australia at the Torino Winter Olympic Games did so following approximately 300 start simulations and about 220 training/competition runs over a period of 14 months. Using a deliberate programming model, these findings provide a guide to the minimum exposure required for a novice skeleton athlete to reach Olympic representative standard following intensified sport-specific training. The findings of this study are discussed in the context of the deliberate practice theory and offer the term "deliberate programming" as an alternative way of incorporating all aspects of expert development.


Assuntos
Aptidão , Desempenho Atlético , Esportes na Neve , Adolescente , Adulto , Austrália , Feminino , Humanos , Adulto Jovem
19.
Sports Biomech ; 7(3): 351-60, 2008 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-18972884

RESUMO

This study characterizes key elements of the start in elite female World Cup skeleton athletes. The top 20 female competitors in three World Cup races were videotaped within a calibrated space to allow the following components of the start to be quantified: (1) acceleration (velocity at 15-m mark, time to 15-m mark), (2) capacity (time to load, total number of steps to load), and (3) load (velocity at 45-m mark). A correlation analysis was used to establish the relationship between the variables of interest and overall start time (15- to 65-m mark). Velocity at the 15-m mark accounted for 86% of the variance in overall start time at St. Moritz and 85% at Sigulda. A stepwise regression analysis revealed that approximately 89% of the variation in start time could be explained by velocity at the 15-m mark, time to load, and velocity at the 45-m mark. Of the variables analysed in this study, rapid acceleration to attain a high velocity at the 15-m mark was the most important component of a fast overall start time. The importance of the time to load and velocity at the 45-m mark vary according to the different track characteristics.


Assuntos
Modelos Biológicos , Tempo de Reação/fisiologia , Esportes na Neve/fisiologia , Análise e Desempenho de Tarefas , Fatores de Tempo , Feminino , Humanos , Internacionalidade , Modelos Estatísticos , Adulto Jovem
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