RESUMO
Empedopeptin is a new antibiotic produced by empedobacter haloabium nov. sp. (ATCC 31962). It is a water-soluble depsipeptide antibiotic containing eight amino acid residues and a C14-fatty acid moiety in the molecule. Although structurally unrelated, empedopeptin and vancomycin have similar antimicrobial spectra against aerobic and anaerobic Gram-positive bacteria including antibiotic-resistant strains. Empedopeptin is highly active in vivo in mice against systemic infections of Staphylococcus aureus, Streptococcus pyogenes and Clostridium perfringens. Empedopeptin is not absorbed orally.
Assuntos
Antibacterianos/isolamento & purificação , Flavobacterium/metabolismo , Oligopeptídeos/isolamento & purificação , Animais , Antibacterianos/biossíntese , Antibacterianos/farmacologia , Fenômenos Químicos , Química , Flavobacterium/classificação , Camundongos , Testes de Sensibilidade Microbiana , Oligopeptídeos/farmacologiaRESUMO
Streptomyces rishiriensis produces at least five closely related antibiotics. Strain selection yielded a culture producing only the most active component, coumermycin A. Hydrolysis of this antibiotic by barium hydroxide yielded both 5-methyl-pyrrole-2-carboxylic acid and pyrrole-2-carboxylic acid, which could be separated by paper chromatography. Coumermycin A was thus shown to be two fractions, designated A(1) and A(2) depending upon the nature of the pyrrole carboxylic acid portion. The addition of cobalt to the fermentation medium at a level as low as 0.01 mug/ml shifted the fermentation exclusively to the production of coumermycin A(1). Other ions were ineffective, except nickel, whose activity could be explained by the presence of contaminating cobalt.
