Critical Issues for Understanding Global Change Effects on Terrestrial Ecosystems.

Marked alterations in the Earth's environment have already been observed, and these presage even greater changes as the impact of human (i.e., land use and industrial) activities increases. Direct and indirect feedbacks link terrestrial ecosystems with global change, and include interactions affecting fluxes of water, energy, nutrients, and "greenhouse" gases and affecting ecosystem structure and composition. Community development can affect ecosystem dynamics by altering resource partitioning among biotic components and through changes in structural characteristics, thereby affecting feedbacks to global change. The response of terrestrial ecosystems to the climate-weather system is dependent on the spatial scale of the interactions between these systems and the temporal scale that links the various components. The International Geosphere-Biosphere Programme (IGBP), which was initiated by the International Council of Scientific Unions (ICSU) in 1986, has undertaken to develop a research plan to address a predictive understanding of how terrestrial ecosystem will be impacted by global changes in the environment and the potential feedbacks. The IGBP science plan, which incorporates established Core Projects and activities related to research on terrestrial ecosystem linkages to global change, includes the International Global Atmospheric Chemistry Project (IGAC); the Biospheric Aspects of the Hydrological Cycle (BAHC); the Global Change and Terrestrial Ecosystems (GCTE); Global Analysis, Integration, and Modelling (GAIM); IGBP Data and Information System (DIS); and IGBP Regional Research Centers (RRC). The coupling of research and policy communities for the purpose of developing mechanisms to adapt to these impending changes urgently needs to be established.

Effects of cadmium, copper, magnesium, and zinc on the decomposition of citrate by a Klebsiella sp.

The effects of Cd2+, Cu2+, Mg2+, and Zn2+ on the decomposition of citric acid by a Klebsiella sp. were studied by monitoring the degradation of [14C]citrate. The carbon concentration used was 10 micrograms of C liter-1, and the media were designed to provide at least 95% of the citrate complexed to the metal studied. After 72 h of incubation, 80% of the uncomplexed citric acid and 76% of the magnesium citrate had been decomposed. A marked inhibition was observed when Cd2+, Cu2+, or Zn2+ was bound to the organic anion; only 23% of the cadmium citrate, 14% of the zinc citrate, and 5% of the cuprous citrate had been decomposed. The effects were not the result of toxicity, since experiments run with [14C]glucose (nonchelating compound) instead of citrate resulted in similar decomposition rates regardless of the presence of the metal. To examine whether the binding of a metal to citrate enhanced its uptake by the Klebsiella sp., we studied the relative uptake of 65Zn in citrate- and in glucose-containing media. No such effect could be observed, with the uptake of Zn2+ being higher in the glucose-containing media. The study shows that metals may render low-molecular-weight organic acids, such as citric acid, resistant to bacterial degradation. This stresses the importance of metals in influencing microbial decomposition of organic compounds, not only as a result of toxicity.

Effects of Glucose Concentrations on Cadmium, Copper, Mercury, and Zinc Toxicity to a Klebsiella sp.

The influence of glucose concentration on Cd, Cu, Hg, and Zn toxicity to a Klebsiella sp. was studied by following the degradation of C-labeled glucose at pH 6.0. Uptake of C into the cells was also determined. The carbon concentrations ranged from 0.01 to 40 mg liter, which are equivalent to soluble C concentrations in natural environments. The toxicity of Cu, Cd, and Zn to a Klebsiella sp. was affected considerably by the C concentration. Copper at 10 M was toxic when the carbon concentration was 10 or 40 mg liter, while at 0.01 to 1.0 mg liter no toxicity was observed. Cadmium and zinc were toxic at 10 M in media containing 0.01 to 1.0 mg of C liter. At C concentrations greater than 1.0 mg liter, the inhibition of glucose degradation and carbon assimilation was observed at 10 M Cd and Zn. The toxicity of mercury seemed to be independent of the C concentration. Results of this study showed that the nutritional state of an organism may have a profound effect on its sensitivity to metals. Metals taken up by an energy-driven transport system may be less toxic under conditions of C starvation. The C concentration should be taken into account when evaluating results from toxicity studies, especially as most microorganisms in nature live under energy-limited conditions.

Seasonal variations in abundance and activity of nitrifiers in four arable cropping systems.

Ammonium and nitrite oxidizers were counted with the most probable number (MPN) method and potential ammonium- and nitrite-oxidation rates were determined with a chlorate inhibition technique in an arable soil over a 3-year period. Samples were taken from the topsoil once a month for 2 years and a few times during a third year in four cropping systems: unfertilized lucerne ley and barley, and nitrate fertilized grass ley and barley. The distribution of nitrifiers was determined and their activities measured at various soil depths and between and within plant rows of fertilized barley.The numbers and activities of ammonium oxidizers were highest in the spring and autumn samples. Numbers of ammonium oxidizers ranged from 0.2 to 19×10(4) and nitrite oxidizers from 3 to 870×10(4) cells g(-1) dry soil. Potential ammonium-oxidizer activities ranged from 120 to 1,060 and nitrite-oxidizer activities ranged from 280 to 680 ng N g(-1) dry soil hour(-1). Lucerne and grass leys generally showed the highest, whereas unfertilized barley had the lowest, abundances and activities.Abundance estimates and activities were 10-20 times higher in the plow layer than in underlying sand and clay layers. A strong correlation was found between organic matter content vs numbers and activities of both ammonium and nitrite oxidizers. Only nitrite oxidizer counts were significantly higher within plant rows compared to between plant rows.

Effects of moisture on soil microorganisms and nematodes: A field experiment.

The effects of soil moisture changes on bacteria, fungi, protozoa, and nematodes and changes in oxygen consumption were studied in a field experiment. In one plot the soil was drip-irrigated daily for 10 days, while an adjacent plot experienced one rainfall and was then allowed to dry out. Oxygen consumption was the parameter measured which responded most rapidly to changes in soil moisture content. Lengths of fluorescein diacetate-active hyphae paralleled oxygen consumption in both plots. Total hyphal length was not affected by one rainfall but increased from 700 mg(-1) dry weight soil to more than 1,600 m in less than 10 days in the irrigated plot. In the rain plot, bacterial numbers doubled within 3 days and declined during the following period of drought. In the irrigated plot, numbers increased by 50% and then remained constant over the duration of the study. Only small changes in protozoan numbers were observed, with the exception of the last sampling date in the irrigated plot when large numbers of naked amoebae were recorded 2 days after a large natural rainfall. Nematode numbers, especially obligate root feeders, increased in both treatments. The increases were caused by decoiling rather than growth. The results indicate that fungal respiration was dominating, while bacteria, lacking a suitable source of energy, were less active, except for the first days.

Fluorescein diacetate hydrolysis as a measure of total microbial activity in soil and litter.

Spectrophotometric determination of the hydrolysis of fluorescein diacetate (FDA) was shown to be a simple, sensitive, and rapid method for determining microbial activity in soil and litter. FDA hydrolysis was studied in soil and straw incubated for up to 3 h. Hydrolysis was found to increase linearly with soil addition. FDA hydrolysis by pure cultures of Fusarium culmorum increased linearly with mycelium addition both in shake cultures and after inoculation into sterile soil. FDA hydrolysis by Pseudomonas denitrificans increased linearly with biomass addition. The FDA hydrolytic activities in soil samples from different layers of an agricultural soil were correlated with respiration. Acetone was found to be suitable for terminating the reaction.