Ultraviolet protection in eye and face protection against biological hazards.

BACKGROUND: In the development of eye and face protection standards against biological hazards, it is important to consider that the eye and face protectors may be used in the outdoor environment, where ultraviolet (UV) exposure from the sun is much higher than indoors. Workers affected include paramedics, ambulance officers and clinical testing personnel. METHODS: Spectral transmittances (280-780 nm) were measured on eight face shields and one goggle of the types used in hospital and clinical environments, three overhead projector sheets and an occupational impact protective goggle. The UV and luminous transmittances were calculated according to the international and two national sunglass standards, and compared with the compliance requirements. RESULTS: All the face shields and goggle lenses were made of variants of the same material. All the samples, including the overhead projector sheets, complied with the requirements of the sunglass standards (for normal conditions, in the case of the American standard). CONCLUSION: Since all the lenses complied with the sunglass standards for UV protection, and there appears to be uniformity in the choice of materials in this product type, there is no need to make provision, as is the case for occupational eye and face protection standards, for protectors that do not provide UV protection. This makes labelling and advice to end users from eyecare and safety professionals much simpler.

Advancing full-field metrology: rapid 3D imaging with geometric phase ferroelectric liquid crystal technology in full-field optical coherence microscopy.

Optical coherence microscopy (OCM) is a variant of OCT in which a high-numerical aperture lens is used. Full-field OCM (FF-OCM) is an emerging non-invasive, label-free, interferometric technique for imaging of surface structures or semi-transparent biomedical subjects with micron-scale resolutions. Different approaches to three dimensional full-field optical metrology are reviewed. The usual method for the phase-shifting technique in FF-OCM involves mechanically moving a mirror to change the optical path difference for obtaining en-face OCM images. However, with the use of a broadband source in FF-OCM, the phase shifts of different spectral components are not the same, resulting in the ambiguities in 3D image reconstruction. In this study, we demonstrate, by imaging tissues and cells, a unique geometric phase-shifter based on ferroelectric liquid crystal technology, to realize achromatic phase-shifting for rapid three-dimensional imaging in a FF-OCM system.

In-vivo corneal confocal microscopy: Imaging analysis, biological insights and future directions.

In-vivo corneal confocal microscopy is a powerful imaging technique which provides clinicians and researcher with the capabilities to observe microstructures at the ocular surfaces in significant detail. In this Mini Review, the optics and image analysis methods with the use of corneal confocal microscopy are discussed. While novel insights of neuroanatomy and biology of the eyes, particularly the ocular surface, have been provided by corneal confocal microscopy, some debatable elements observed using this technique remain and these are explored in this Mini Review. Potential improvements in imaging methodology and instrumentation are also suggested.

Feasibility of Silicon Quantum Dots as a Biomarker for the Bioimaging of Tear Film.

This study investigated the fluorescence and biocompatibility of hydrophilic silicon quantum dots (SiQDs) that are doped with scandium (Sc-SiQDs), copper (Cu-SiQDs), and zinc (Zn-SiQDs), indicating their feasibility for the bioimaging of tear film. SiQDs were investigated for fluorescence emission by the in vitro imaging of artificial tears (TheraTears®), using an optical imaging system. A trypan blue exclusion test and MTT assay were used to evaluate the cytotoxicity of SiQDs to cultured human corneal epithelial cells. No difference was observed between the fluorescence emission of Sc-SiQDs and Cu-SiQDs at any concentration. On average, SiQDs showed stable fluorescence, while Sc-SiQDs and Cu-SiQDs showed brighter fluorescence emissions than Zn-SiQDs. Cu-SiQDs and Sc-SiQDs showed a broader safe concentration range than Zn-SiQDs. Cu-SiQDs and Zn-SiQDs tend to aggregate more substantially in TheraTears® than Sc-SiQDs. This study elucidates the feasibility of hydrophilic Sc-SiQDs in studying the tear film's aqueous layer.

Glaucoma diagnosis using multi-feature analysis and a deep learning technique.

In this study, we aimed to facilitate the current diagnostic assessment of glaucoma by analyzing multiple features and introducing a new cross-sectional optic nerve head (ONH) feature from optical coherence tomography (OCT) images. The data (n = 100 for both glaucoma and control) were collected based on structural, functional, demographic and risk factors. The features were statistically analyzed, and the most significant four features were used to train machine learning (ML) algorithms. Two ML algorithms: deep learning (DL) and logistic regression (LR) were compared in terms of the classification accuracy for automated glaucoma detection. The performance of the ML models was evaluated on unseen test data, n = 55. An image segmentation pilot study was then performed on cross-sectional OCT scans. The ONH cup area was extracted, analyzed, and a new DL model was trained for glaucoma prediction. The DL model was estimated using five-fold cross-validation and compared with two pre-trained models. The DL model trained from the optimal features achieved significantly higher diagnostic performance (area under the receiver operating characteristic curve (AUC) 0.98 and accuracy of 97% on validation data and 96% on test data) compared to previous studies for automated glaucoma detection. The second DL model used in the pilot study also showed promising outcomes (AUC 0.99 and accuracy of 98.6%) to detect glaucoma compared to two pre-trained models. In combination, the result of the two studies strongly suggests the four features and the cross-sectional ONH cup area trained using deep learning have a great potential for use as an initial screening tool for glaucoma which will assist clinicians in making a precise decision.

Modelling the effect of light through commercially available blue-blocking lenses on the human circadian system.

CLINICAL RELEVANCE: Wearing blue-blocking lenses (BBLs) in the evening hours may not be effective in improving sleep quality. Optometrists need to be informed in prescribing BBLs by highlighting the consequences of their wear to the circadian system. BACKGROUND: Excessive exposure to artificial light, particularly at short wavelengths, during the evening, may disrupt normal nocturnal melatonin production, which is a natural process of the circadian rhythm and affect sleep quality. Current BBLs have been designed to limit blue-light exposure and may offer a means to minimise disruption to the circadian system. The purpose of this study was to evaluate the impact of BBLs on a normal sleep-wake circadian rhythm. METHODS: Seven different commercial brands of BBLs (Crizal Prevencia, Smart Blue Filter, Blu-OLP, Blue Control, UV++Blue Control, SeeCoat Blue UV and Blue Guardian) and powers (+2.00 D, -2.00 D and Plano) were evaluated by quantifying the degree to which they reduce light radiation from lamps and electronic devices. In particular, the non-linear circadian index and the circadian stimulus was determined for various light sources to establish changes in melatonin production that occur while viewing through different BBLs. RESULTS: A large difference was shown in the effectiveness of different BBL brands in reducing the spectral sensitivity of the circadian system. The BBL brand was shown to selectively affect the non-linear circadian index and circadian stimulus, particularly with those with transmittance profiles that block the most blue light having the lowest effect on the suppression of nocturnal melatonin secretion. CONCLUSION: BBLs may not improve sleep quality, because they continue to allow the transmittance of blue light that may suppress nocturnal melatonin secretion and hence disrupt the normal sleep-wake circadian rhythm.

Genome-wide identification and prediction of SARS-CoV-2 mutations show an abundance of variants: Integrated study of bioinformatics and deep neural learning.

Genomic data analysis is a fundamental system for monitoring pathogen evolution and the outbreak of infectious diseases. Based on bioinformatics and deep learning, this study was designed to identify the genomic variability of SARS-CoV-2 worldwide and predict the impending mutation rate. Analysis of 259044 SARS-CoV-2 isolates identified 3334545 mutations with an average of 14.01 mutations per isolate. Globally, single nucleotide polymorphism (SNP) is the most prevalent mutational event. The prevalence of C > T (52.67%) was noticed as a major alteration across the world followed by the G > T (14.59%) and A > G (11.13%). Strains from India showed the highest number of mutations (48) followed by Scotland, USA, Netherlands, Norway, and France having up to 36 mutations. D416G, F106F, P314L, UTR:C241T, L93L, A222V, A199A, V30L, and A220V mutations were found as the most frequent mutations. D1118H, S194L, R262H, M809L, P314L, A8D, S220G, A890D, G1433C, T1456I, R233C, F263S, L111K, A54T, A74V, L183A, A316T, V212F, L46C, V48G, Q57H, W131R, G172V, Q185H, and Y206S missense mutations were found to largely decrease the structural stability of the corresponding proteins. Conversely, D3L, L5F, and S97I were found to largely increase the structural stability of the corresponding proteins. Multi-nucleotide mutations GGG > AAC, CC > TT, TG > CA, and AT > TA have come up in our analysis which are in the top 20 mutational cohort. Future mutation rate analysis predicts a 17%, 7%, and 3% increment of C > T, A > G, and A > T, respectively in the future. Conversely, 7%, 7%, and 6% decrement is estimated for T > C, G > A, and G > T mutations, respectively. T > G\A, C > G\A, and A > T\C are not anticipated in the future. Since SARS-CoV-2 is mutating continuously, our findings will facilitate the tracking of mutations and help to map the progression of the COVID-19 intensity worldwide.

The effect of blue light filtering lenses on speed perception.

Blue-light filtering lenses (BFLs) are marketed to protect the eyes from blue light that may be hazardous to the visual system. Because BFLs attenuate light, they reduce object contrast, which may impact visual behaviours such as the perception of object speed which reduces with contrast. In the present study, we investigated whether speed perception is affected by BFLs. Using a two-interval forced-choice procedure in conjunction with Method of Constant Stimuli, participants (n = 20) judged whether the perceived speed of a moving test stimulus (1.5-4.5°/s) viewed through a BFL was faster than a reference stimulus (2.75°/s) viewed through a clear lens. This procedure was repeated for 3 different BFL brands and chromatic and achromatic stimuli. Psychometric function fits provided an estimate of the speed at which both test and reference stimuli were matched. We find that the perceived speed of both chromatic and achromatic test stimuli was reduced by 6 to 20% when viewed through BFLs, and lenses that attenuated the most blue-light produced the largest reductions in perceived speed. Our findings indicate that BFLs whilst may reduce exposure to hazardous blue light, have unintended consequences to important visual behaviours such as motion perception.

Effect of blue-blocking lenses on colour contrast sensitivity.

CLINICAL RELEVANCE: There is a significant unintended consequences of blue-blocking lenses on visual behaviour, particularly for the detection of colour. Optometrists need to be mindful of this when prescribing the appropriate blue-blocking lenses for individuals who work in environments in which blue light is prevalent. BACKGROUND: The selective reduction in visible wavelengths transmitted through commercially available blue-blocking lenses is known to influence object appearance and luminance contrast, and also potentially object colour contrast. The present study investigated the effect of a number of commercially available blue-blocking lenses on colour contrast sensitivity in normal individuals under low and high contrast stimulus conditions. METHODS: Five healthy participants (one man and four women), aged between 23 and 39-years, were recruited for this study. Crizal Prevencia (Essilor), Blue Guardian (Opticare), and Blu-OLP (GenOp) lenses were examined in this study in comparison to a control lens (clear lens without blue-filtering coating). In Experiment 1, colour contrast thresholds were measured using a visual search colour detection task in which the colour (CIE Lu'v' red, green, blue and yellow) of the target circle stimulus (randomly located in an annulus of achromatic circles) was systematically reduced using a staircase procedure. As blue-blocking lenses selectively block blue light, in Experiment 2, colour contrast thresholds were specifically quantified for a range of short wavelengths near the attenuation transmittance range of the blue-blocking lenses tested. RESULTS: Experiments 1 and 2 showed that colour contrast was impaired only for blue colours, and this was most evident at low contrasts. Additionally, the blue-blocking lenses with lower transmittance profiles led to greater reductions in colour contrast sensitivity and shown to affect colour contrast thresholds. CONCLUSION: Our results suggest that while reducing blue light potentially minimises the harmful effect of blue hazard light, blue-blocking lenses can unintentionally reduce colour contrast sensitivity, particularly at low light levels.

Effect of blue-blocking lenses on colour discrimination.

CLINICAL RELEVANCE: Tinted lenses may adversely affect colour discrimination. Before recommending tinted lenses to patients, practitioners should bear in mind any effects on colour discrimination. The effects of 'blue-blocking' spectacle lenses with high luminous transmittance on colour vision is not a concern. BACKGROUND: Blue-blocking lenses have been widely promoted by manufacturers and practitioners. The more blue-blocking lenses are known to affect colour vision significantly but there has been no study of the effects of 'blue-blocking' spectacle lenses. METHODS: The transmittances of commercially available lenses were measured and the three lenses with the lowest blue light transmittance were selected. Subjects undertook the following computer-based colour vision tests: Colour Assessment and Diagnosis; the Cambridge Colour Test; and the Farnsworth-Munsell 100 Hue Test. RESULTS: Blue and luminous transmittances of lenses were documented. The reduction in blue transmittance varied from 12 to 40 per cent (two to 30 per cent compared with an untinted lens). The lenses were found to have no significant, statistical or possibly practical, effect on the results of the three colour vision tests (t-test, analysis of variance, Mann-Whitney, Kruskal-Wallis). CONCLUSION: The modest blue light transmittance reduction of the 'blue-blocking' spectacle lenses examined was not sufficient to have a statistically significant effect on colour vision.

The Effect of Blue-blocking Lenses on Photostress Recovery Times.

SIGNIFICANCE: The selective reduction in visible wavelengths transmitted through commercially available blue-blocking lenses (BBLs) is known to influence the appearance and contrast detection of objects, particularly at low light levels. This influence may impair the human retinal receptor response time to dynamic light changes during photostress events. PURPOSE: This study aimed to assess whether BBLs selectively affect photostress recovery times (PSRTs) for chromatic and achromatic stimuli of different Weber contrasts that were viewed on a dark black background. METHODS: Photostress recovery times were measured in 12 younger participants (18 to 39 years old) with no history of ocular disease or abnormal vision. Photostress recovery times were evaluated for four brands of BBLs, which were compared with a control lens. In these experiments, after exposure to an intense light source for 5 seconds, the time taken to recover vision and correctly identify a computer-generated letter stimulus viewed under low and high luminance levels was determined, which means perception is likely to be governed by mesopic and photopic conditions. Across conditions, the letter stimulus was achromatic and chromatic and could differ in luminance contrast. RESULTS: Under photopic stimulus conditions, although reducing luminance contrast increased PSRTs, BBLs had no significant effect on PSRTs relative to control lens. However, under mesopic stimulus conditions, BBLs significantly affect PSRTs for both achromatic (F2.006,8.02 = 61.95, P < .0001) and chromatic stimuli (F3,16 =139.01, P < .0001), particularly for blue targets, which had considerably longer PSRTs (38.40 seconds). The brand of BBL was also shown to selectively affect PSRTs, with those with transmittance profiles that block the most blue light having longer PSRTs. CONCLUSIONS: The present study suggests that, although the color and contrast of the target stimuli affected recovery times, the difference in recovery times between different types of BBLs was noticed only under low-light-level stimulus conditions.

Hard x-ray phase-contrast microscopy using a Gabor hologram without a zero-order term.

In order to achieve a nanometer-scale resolution in an x-ray microscopy system, a Gabor-type hologram was produced by eliminating the zero-order term of the object diffraction pattern. In this system, a Fresnel zone plate was used for strong illumination of an object, and the zero-order diffraction was physically eliminated by a center stop. An accurate phase plate of π/2 in the Zernike method was numerically created, and the phase-contrast image was realized. The theoretical resolution was 19.8 nm. We proved that a gold nanocube with a size of 50 nm can be reconstructed with the predicted resolution.

Modelling the effect of commercially available blue-blocking lenses on visual and non-visual functions.

BACKGROUND: Blue-blocking lenses (BBLs) are marketed as providing retinal protection from acute and cumulative exposure to blue light over time. The selective reduction in visible wavelengths transmitted through BBLs is known to influence the photosensitivity of retinal photoreceptors, which affects both visual and non-visual functions. This study measured the spectral transmittance of BBLs and evaluated their effect on blue perception, scotopic vision, circadian rhythm, and protection from photochemical retinal damage. METHODS: Seven different types of BBLs from six manufacturers and untinted control lenses with three different powers (+2.00 D, -2.00 D and Plano) were evaluated. The whiteness index of BBLs used in this study was calculated using Commission International de l'Eclairage (CIE) Standard Illuminates D65, and CIE 1964 Standard with a 2° Observer. The protective qualities of BBLs and their effect on blue perception, scotopic vision, and circadian rhythm were evaluated based on their spectral transmittance, which was measured with a Cary 5,000 UV-Vis-NIR spectrophotometer. RESULTS: BBLs were found to reduce blue light (400-500 nm) by 6-43 per cent, providing significant protection from photochemical retinal damage compared to control lenses (p ≤ 0.05). All BBLs were capable of reducing the perception of blue colours, scotopic sensitivities and circadian sensitivities by 5-36 per cent, 5-24 per cent, and 4-27 per cent, respectively depending on the brand and power of the lens. CONCLUSION: BBLs can provide some protection to the human eye from photochemical retinal damage by reducing a portion of blue light that may affect visual and non-visual performances, such as those critical to scotopic vision, blue perception, and circadian rhythm.

Quantum Dots in Ophthalmology: A Literature Review.

Purpose: The aim of the current review was to summarize the current applications, the latest advances and importantly, highlight research gaps in the use of quantum dots in the eye. Quantum dots are nanoscale semiconductor crystals with characteristic size and tunable optical properties, which deliver bright and stable fluorescence suitable for bioimaging and labelling. Methods: A systematic search was conducted following the PRISMA guidelines. This review systematically searched published data to summarize the characteristics and applications of quantum dots in ophthalmology. Two hundred and eighty published articles were initially selected for this review following searches using the criteria quantum dots AND nanoparticles AND ophthalmology in the databases PubMed, MEDLINE, Scopus, Embase and Web of Science. Results: After duplicates were removed, a total of 22 eligible articles were included for the review. Quantum dots potentially provide a range of diagnostic and therapeutic applications in ophthalmology. Quantum dots offer visible and near-infrared emission, which is highly desirable for bioimaging, due to reduced light scattering and low tissue absorption. Their applications include in vivo bioimaging, labelling of cells and tissues, delivery of genes or drugs and as antimicrobial composites. Conclusion: Quantum dots have been used in ophthalmology for bioimaging, electrical stimulation and tracking of gene/stems cells, and ocular lymphatics. However, there is no detailed description of their desirable characteristics for use in ophthalmology, and there is limited information about their cytotoxicity to ocular cells and tissues.

Role of random biopsies in surveillance of dysplasia in ulcerative colitis patients with high risk of colorectal cancer.

BACKGROUND/AIMS: Recent data suggest that the incidence of ulcerative colitis (UC) related colorectal cancer (CRC) in India is similar to that of West. The optimum method for surveillance is still a debate. Surveillance with random biopsies has been the standard of care, but is a tedious process. We therefore undertook this study to assess the yield of random biopsy in dysplasia surveillance. METHODS: Between March 2014 and July 2015, patients of UC attending the Inflammatory Bowel Disease clinic at the All India Institute of Medical Sciences with high risk factors for CRC like duration of disease >15 years and pancolitis, family history of CRC, primary sclerosing cholangitis underwent surveillance colonoscopy for dysplasia. Four quadrant random biopsies at 10 cm intervals were taken (33 biopsies). Two pathologists examined specimens for dysplasia, and the yield of dysplasia was calculated. RESULTS: Twenty-eight patients were included. Twenty-six of these had pancolitis with a duration of disease greater than 15 years, and two patients had associated primary sclerosing cholangis. No patient had a family history of CRC. The mean age at onset of disease was 28.89±8.73 years and the duration of disease was 19.00±8.78 years. Eighteen patients (64.28%) were males. A total of 924 biopsies were taken. None of the biopsies revealed any evidence of dysplasia, and 7/924 (0.7%) were indefinite for dysplasia. CONCLUSIONS: Random biopsy for surveillance in longstanding extensive colitis has a low yield for dysplasia and does not suffice for screening. Newer techniques such as chromoendoscopy-guided biopsies need greater adoption.

Assessing the aggregation behaviour of iron oxide nanoparticles under relevant environmental conditions using a multi-method approach.

Iron nanoparticles are becoming increasingly popular for the treatment of contaminated soil and groundwater; however, their mobility and reactivity in subsurface environments are significantly affected by their tendency to aggregate. Assessing their stability under environmental conditions is crucial for determining their environmental fate. A multi-method approach (including different size-measurement techniques and the DLVO theory) was used to thoroughly characterise the behaviour of iron oxide nanoparticles (Fe2O3NPs) under environmentally relevant conditions. Although recent studies have demonstrated the importance of using a multi-method approach when characterising nanoparticles, the majority of current studies continue to use a single-method approach. Under some soil conditions (i.e. pH 7, 10 mM NaCl and 2 mM CaCl2) and increasing particle concentration, Fe2O3NPs underwent extensive aggregation to form large aggregates (>1 µm). Coating the nanoparticles with dissolved organic matter (DOM) was investigated as an alternative "green" solution to overcoming the aggregation issue instead of using the more commonly proposed polyelectrolytes. At high concentrations, DOM effectively covered the surface of the Fe2O3NPs, thereby conferring negative surface charge on the particles across a wide range of pH values. This provided electrostatic stabilisation and considerably reduced the particle aggregation effect. DOM-coated Fe2O3NPs also proved to be more stable under high ionic strength conditions. The presence of CaCl2, however, even at low concentrations, induced the aggregation of DOM-coated Fe2O3NPs, mainly via charge neutralisation and bridging. This has significant implications in regards to the reactivity and fate of these materials in the environment.

Characterisation of Fe-oxide nanoparticles coated with humic acid and Suwannee River natural organic matter.

Iron oxide nanoparticles are becoming increasingly popular for various applications including the treatment of contaminated soil and groundwater; however, their mobility and reactivity in the subsurface environment are significantly affected by their tendency to aggregate. One solution to overcome this issue is to coat the nanoparticles with dissolved organic matter (DOM). The advantages of DOM over conventional surface modifiers are that DOM is naturally abundant in the environment, inexpensive, non-toxic and readily adsorbed onto the surface of metal oxide nanoparticles. In this study, humic acid (HA) and Suwannee River natural organic matter (SRNOM) were tested and compared as surface modifiers for Fe2O3 nanoparticles (NPs). The DOM-coated Fe2O3 NPs were characterised by various analytical methods including: flow field-flow fractionation (FlFFF), high performance size exclusion chromatography (HPSEC) and Fourier transform infrared spectroscopy (FTIR). The stability of the coated NPs was then evaluated by assessing their aggregation and disaggregation behaviour over time. Results showed that both HA and SRNOM were rapidly and readily adsorbed on the surface of Fe2O3 NPs, providing electrosteric stabilisation over a wide range of pH. HPSEC results showed that the higher molecular weight components of DOM were preferentially adsorbed onto the surface of Fe2O3. As SRNOM consists of macromolecules with a higher molecular weight than HA, the measured size of the SRNOM-coated Fe2O3 NPs was 30% larger than the HA-coated Fe2O3 NPs. FTIR results indicated the occurrence of hydrogen bonding arising from electrostatic interaction between the DOM and Fe2O3 NPs. Finally, a stability study showed that after 14 days, small agglomerates and aggregates were formed. The HA-coated Fe2O3 NPs formed agglomerates which were easily disaggregated using a vortex mixer, with the coated NPs returning to their initial size. However, SRNOM-coated Fe2O3 NPs were only partially disaggregated using the same method, which indicates that these aggregates have a more compact structure.

White-light interference microscopy: minimization of spurious diffraction effects by geometric phase-shifting.

A common problem when profiling surfaces with steps or discontinuities using white-light (coherence-probe) interferometry is localized spikes (batwings) or spurious peaks due to diffraction effects. We show that errors due to these effects can be minimized by processing the irradiance data obtained with an achromatic phase-shifter operating on the geometric (Pancharatnam) phase to yield the values of the surface height.

White-light interference microscopy: a way to obtain high lateral resolution over an extended range of heights.

A problem with conventional techniques of interference microscopy, when profiling surfaces with an extended range of heights, is that only points on a single plane are in sharp focus. Other points, which are higher or lower, may be out of focus, with a consequent loss of lateral resolution. We show that white-light interference microscopy, with an achromatic phase-shifter, makes it possible to produce a three-dimensional representation of such surfaces with high lateral resolution over the entire range of heights.