E41K Mutation Activates Bruton's Tyrosine Kinase by Stabilizing an Inositol Hexakisphosphate dependent Invisible Dimer.

Bruton's tyrosine kinase (BTK) regulates diverse cellular signaling of the innate and adaptive immune system in response to microbial pathogens. Downregulation or constitutive activation of BTK is reported in patients with autoimmune diseases or various B-cell leukemias. BTK is a multidomain protein tyrosine kinase that adopts an Src-like autoinhibited conformation maintained by the interaction between the kinase and PH-TH domains. The PH-TH domain plays a central role in regulating BTK function. BTK is activated by binding to PIP3 at the plasma membrane upon stimulation by the B-cell receptor (BCR). The PIP3 binding allows dimerization of the PH-TH domain and subsequent transphosphorylation of the activation loop. Alternatively, a recent study shows that the multivalent T-cell-independent (TI) antigen induces BCR response by activating BTK independently of PIP3 binding. It was proposed that a transiently stable IP6-dependent PH-TH dimer may activate BTK during BCR activation by the TI antigens. However, no IP6-dependent PH-TH dimer has been identified yet. Here, we investigated a constitutively active PH-TH mutant (E41K) to determine if the elusive IP6-dependent PH-TH dimer exists. We showed that the constitutively active E41K mutation activates BTK by stabilizing the IP6-dependent PH-TH dimer. We observed that a downregulating mutation in the PH-TH domain (R28H) linked to X-linked agammaglobulinemia impairs BTK activation at the membrane and in the cytosol by preventing PH-TH dimerization. We conclude that the IP6 dynamically remodels the BTK active fraction between the membrane and cytoplasm. Stimulating with IP6 increases the cytosolic fraction of the activated BTK.

Starch-based biodegradable films amended with nano-starch and tannic acid-coated nano-starch exhibit enhanced mechanical and functional attributes with antimicrobial activity.

Starch-based biofilms are biodegradable, but their application is limited by lower mechanical strength and absence of antimicrobial properties. In this context, the present study attempted to unleash the potential of nanotechnology for synthesizing nano-starch (NS) and tannic acid-coated nano-starch (T-NS) for augmenting the tensile strength and antimicrobial properties of starch-based biofilms. Moreover, this study reports one of the first such attempts to improve the commercial viability of starch extracted from the corms of Amorphophallus paeoniifolius. In this study, NS and T-NS samples were first synthesized by the physical and chemical modification of the native starch (S) molecules. The NS and T-NS samples showed significantly smaller granule size, lower moisture content, and swelling power. Further, amendments with NS and T-NS samples (25 % and 50 %) to the native starch molecules were performed to obtain biofilm samples. The NSB (NS amended) and T-NSB (T-NS amended) biofilms showed comparatively higher tensile strength than SB films (100 % starch-based). The T-NSB showed greater antimicrobial activity against gram-positive and gram-negative bacteria. All the biofilms showed almost complete biodegradation in soil (in 10 days). Therefore, it can be concluded that additives like NS and T-NS can improve starch-based biofilms' mechanical strength and antimicrobial properties with considerable biodegradability.

Zinc-loaded mesoporous silica nanoparticles mitigate salinity stress in wheat seedlings through silica-zinc uptake, osmotic balance, and ROS detoxification.

Abiotic stresses like salinity and micronutrient deficiency majorly affect wheat productivity. Applying mesoporous silica nanoparticles (MSiNPs) as a smart micronutrient delivery system can facilitate better stress management and nutrient delivery. In this purview, we investigated the potential of MSiNPs and Zn-loaded MSiNPs (Zn-MSiNPs) on the growth and physiology of wheat seedlings exposed to salinity stress (200 mM NaCl). Initially, the FESEM, DLS, and BET analysis portrayed nanoparticles' spherical shape, nano-size, and negatively charged mesoporous surface. A sustained release of Zn+2 from Zn-MSiNPs at 30 °C, diffused light, and pH 7 was perceived with a 96.57% release after 10 days. Further, the mitigation of NaCl stress in the wheat seedlings was evaluated with two different concentrations, each of MSiNPs and Zn-MSiNPs (1 g/L and 5 g/L), respectively. A meticulous improvement in the germination and growth of wheat seedlings was observed when treated with both MSiNPs and Zn-MSiNPs. A considerable increase in chlorophyll, total protein, and sugar content was in consort with a substantial decline in MDA, electrolyte leakage, and ROS accumulation, showcasing the nanomaterials' palliating effects. Most importantly, the K+/Na+ ratio in shoots increased significantly by 3.43 and 4.37 folds after being treated with 5 g/L Zn-MSiNPs, compared to their respective control sets (0 and 200 mM NaCl). Therefore, it can be concluded that the Zn-MSiNPs can effectively restrain the effects of salinity stress on wheat seedlings.

An assessment of nanotechnology-based interventions for cleaning up toxic heavy metal/metalloid-contaminated agroecosystems: Potentials and issues.

Heavy metals (HMs) are among the most dangerous environmental variables for a variety of life forms, including crops. Accumulation of HMs in consumables and their subsequent transmission to the food web are serious concerns for scientific communities and policy makers. The function of essential plant cellular macromolecules is substantially hampered by HMs, which eventually have a detrimental effect on agricultural yield. Among these HMs, three were considered, i.e., arsenic, cadmium, and chromium, in this review, from agro-ecosystem perspective. Compared with conventional plant growth regulators, the use of nanoparticles (NPs) is a relatively recent, successful, and promising method among the many methods employed to address or alleviate the toxicity of HMs. The ability of NPs to reduce HM mobility in soil, reduce HM availability, enhance the ability of the apoplastic barrier to prevent HM translocation inside the plant, strengthen the plant's antioxidant system by significantly enhancing the activities of many enzymatic and nonenzymatic antioxidants, and increase the generation of specialized metabolites together support the effectiveness of NPs as stress relievers. In this review article, to assess the efficacy of various NP types in ameliorating HM toxicity in plants, we adopted a 'fusion approach', in which a machine learning-based analysis was used to systematically highlight current research trends based on which an extensive literature survey is planned. A holistic assessment of HMs and NMs was subsequently carried out to highlight the future course of action(s).

Green synthesis of copper nanoparticles by using pineapple peel waste: in vitro characterizations and antibacterial potential.

A considerable amount of fruit waste is being produced every day worldwide. The green synthesis of metal nanoparticles from fruit peel waste can be an innovative, cost-effective, and eco-friendly alternative to traditional methods. Copper nanoparticles (CuNPs) were synthesized by a green method using the pineapple peels extract (PLX) and copper sulfate pentahydrate. The formation of CuNPs was visually identified and detected by UV-Visible spectroscopy. The CuNPs were characterized by Fourier-transform infrared (FTIR) spectroscopy, particle size analyzer, scanning electron microscopy (SEM), and X-ray diffraction (XRD). The antioxidant and reducing power of CuNPs were conducted by %DPPH scavenging and electron transfer-based ferric reducing antioxidant power (FRAP) assay, respectively. The antibacterial properties of CuNPs were determined in gram-positive, and gram-negative bacteria. The results showed that the CuNPs were spherical in shape with mean particle size 290.5 nm. The zeta potential of the nanoparticles was found to be - 12.3 mV indicating the instability in the colloidal state. The FTIR study confirmed the peaks of phytochemicals present in the PLX and the nanoparticles supporting the use of pineapple peels as stabilizing, reducing and capping agents. Both the DPPH and reducing power assay depicted that the synthesized CuNPs had significant antioxidant activity. However, the synthesized CuNPs had strong inhibitory capacity against both gram-positive and gram-negative test organisms. Thus, the CuNPS could be used for its viable antibacterial potential to preserve fruits, flowers, and vegetables from bacterial contamination.

Synthesis of Spherical Mn2O3 Nanozymes from Different Green Precursors for their Innovative Applications in Catalytic Properties and Bioactivity.

Here, spherical Mn2O3 nanozymes were synthesized via a one-step green method using different green precursors, and their physicochemical properties and biological activities were monitored with various green precursors. Powder X-ray diffraction (PXRD) was performed to determine the crystalline properties and phases involved in the formation of cubic Mn2O3 nanozymes. The synthesized nanozymes were spherical and examined by SEM and FESEM studies. All of the samples synthesized using different green precursors exhibited different sizes but similar spherical shapes. Moreover, all green-synthesized nanozymes catalyzed the oxidation reaction of the chromogenic substrate 3,3'5,5' tetramethylbenzidine (TMB) in the absence of H2O2, and A2 (lemon-mediated Mn2O3 nanozymes), which the followed Michaelis-Menten kinetics, showed the best activity. Therefore, A2 (lemon-mediated nanozyme) showed oxidase-mimicking activity with distinct Km and Vmax values calculated by the Lineweaver-Burk plot. Furthermore, the current nanozymes demonstrated a significant ability to kill both Gram-negative and Gram-positive bacteria as well as effectively destroy biofilms under physiological conditions. Moreover, the green-mediated nanozymes also displayed ROS-scavenging activity. Our nanozymes exhibited scavenging activity toward OH and O2-• radicals and metal chelation activity, which were investigated colorimetrically. Therefore, these nanozymes might be used as effective antibacterial agents and also for the consumption of reactive oxygen species.

Enhanced adaptation to salinity stress in lentil seedlings through the use of trehalose-functionalized silica nanoparticles (TSiNPs): Exploring silica-sugar absorption and oxidative balance.

Silica nanoparticles (SiNPs) confer better growth and development of plants under salinity stress. Moreover, the surface-functionalization of SiNPs with bioactive molecules is expected to enhance its efficacy. The present study thus aimed to modify the surface of SiNPs, by attaching a bioactive molecule (trehalose) to obtain TSiNPs. The successful surface functionalization was confirmed using FTIR, XRD, and EDS. The spherical shape and amorphous nature of the nanoparticles were confirmed using SEM. The TEM image analysis revealed that the size of SiNPs and TSiNPs ranged between 20-50 nm and 200-250 nm, respectively. A novel bioassay experiment designed to study the release of silica and trehalose from nanoparticles elucidated that the TSiNPs improved the release and uptake of silica. Also, trehalose uptake significantly improved after 72 h of application due to enhanced release of trehalose from TSiNPs. Further, this study also aimed to investigate the potential benefits of SiNPs and TSiNPs in promoting the growth and development of plants under salinity stress. In this context, the nanoparticles were applied to the saline-stressed (0, 200, 300 mM) lentil seedlings for the in-planta experiments. The results revealed that both SiNPs and TSiNPs improved the growth of seedlings (shoot, and root length), ionic balance (K+/Na+ ratio), and osmolyte status (sugars, proline, glycine betaine, trehalose). Additionally, increased antioxidant enzyme activities helped scavenge ROS (H2O2, O2.-) generated in NaCl-stressed seedlings, ultimately improving the membrane integrity (by reducing MDA and EL). However, the TSiNPs exhibited a much-enhanced activity in stress alleviation compared to the SiNPs.

Metabolome profile variation in Azolla filiculoides exposed to Bisphenol A assists in the identification of stress-responsive metabolites.

This study attempted to explore the metabolome profile of Azolla filiculoides subjected to two different concentrations of BPA (1 and 30 mg L-1) in congruence with two different durations (3 and 9 days) of treatment. Bisphenol A (BPA) is a ubiquitously occurring environmental pollutant that imparts acute toxicity in aquatic plants. Therefore, studying the variations in the fern metabolome profile and identifying stress-responsive metabolites can help develop criteria for assessing the aquatic ecosystem. In recent times, metabolomics has drawn attention for its ability to detect biochemical processes and help link plant responses with environmental stresses. However, the studies concerning the metabolome profile of A. filiculoides exposed to environmental contaminants are limited. In the present study, the untargeted metabolomics study allowed the detection of a large array of metabolites, with 767 shared metabolites representing 41 crucial pathways. Exposure to 30 mg L-1 BPA seemingly disrupted the primary metabolism of the fern and induced a shift toward defense-related pathways. Additionally, BPA stress triggered the expression of metabolites like 3,4-dihydroxyphenylglycol, perillic acid, and perillaldehyde in BPA_L3 (1 mg L-1 for 3 days) and BPA_L9 (1 mg L-1 for 9 days) samples indicating protective mechanism of the plants. Conversely, the BPA_H3 (30 mg L-1 for 3 days) and BPA_H9 (30 mg L-1 for 9 days) samples expressed a distinct set of markers like luteolin, 3-hydroxyanthranilic acid, cinnamaldehyde, and l-DOPA indicating the onset of senescence and apoptosis related pathways can help in the health assessment of freshwater ecosystems and also appraisal of ecotoxicological risks imposed by BPA.

Papaya peel extract-mediated green synthesis of zinc oxide nanoparticles and determination of their antioxidant, antibacterial, and photocatalytic properties.

This study describes an effective and eco-friendly approach to the synthesis of zinc oxide nanoparticles (ZnONPs) utilizing papaya fruit peel extract (PPE). The structural evaluation and morphological features of synthesized ZnONPs were examined using various physicochemical analyses. The formulated ZnONPs were spherical to hexagonal in shape with ⁓ 170 nm in diameter. ZnONPs exhibited improved antioxidant potential in terms of DPPH radical scavenging activity (IC50 = 98.74 µg/ml) and ferric-reducing potential compared with PPE. The antibacterial activity of ZnONPs was measured against pathogenic strains of Salmonella typhi, Bacillus subtilis, Staphylococcus aureus, and Escherichia coli. The biosynthesized ZnONPs showed potential antibacterial efficacy against all microbes. In addition, ZnONPs exhibited potential photocatalytic activity in rhodamine B degradation in the presence of sunlight. The results indicated that papaya peels, which are these fruit wastes, could be helpful for the green synthesis of ZnONPs with good dose-responsive antioxidant, antibacterial, and photocatalytic activities.

Revisiting Changes in Growth, Physiology and Stress Responses of Plants under the Effect of Enhanced CO2 and Temperature.

Climate change has universally affected the whole ecosystem in a unified manner and is known to have improbable effects on agricultural productivity and food security. Carbon dioxide (CO2) and temperature are the major environmental factors that have been shown to increase sharply during the last century and are directly responsible for affecting plant growth and development. A number of previous investigations have deliberated the positive effects of elevated CO2 on plant growth and development of various C3 crops, while detrimental effects of enhanced temperature on different crop plants like rice, wheat, maize and legumes are generally observed. A combined effect of elevated CO2 and temperature has yet to be studied in great detail; therefore, this review attempts to delineate the interactive effects of enhanced CO2 and temperature on plant growth, development, physiological and molecular responses. Elevated CO2 maintains leaf photosynthesis rate, respiration, transpiration and stomatal conductance in the presence of elevated temperature and sustains plant growth and productivity in the presence of both these environmental factors. Concomitantly, their interaction also affects the nutritional quality of seeds and leads to alterations in the composition of secondary metabolites. Elevated CO2 and temperature modulate phytohormone concentration in plants, and due to this fact, both environmental factors have substantial effects on abiotic and biotic stresses. Elevated CO2 and temperature have been shown to have mitigating effects on plants in the presence of other abiotic stress agents like drought and salinity, while no such pattern has been observed in the presence of biotic stress agents. This review focuses on the interactive effects of enhanced CO2 and temperature on different plants and is the first of its kind to deliver their combined responses in such detail.

Phase variation of manganese oxide in the MnO@ZnO nanocomposite with calcination temperature and its effect on structural and biological activities.

Having powerful antibacterial and antioxidant effects, zinc oxide and manganese oxide nanomaterials are of great interest. Here we have synthesized manganese oxide decorated zinc oxide (MZO) nanocomposites by co-precipitation method, calcined at different temperatures (300-750 °C) and studied various properties. Here the crystalline structure of the nanocomposite and phase change of the manganese oxide are observed with calcination temperature. The average crystalline size increases and the dislocation density and microstrain decrease with the increase in calcined temperature for the same structural features. The formation of composites was confirmed by XRD pattern and SEM images. EDAX spectra proved the high purity of the composites. Here, different biological properties change with the calcination temperature for different shapes, sizes and structures of the nanocomposite. Nanomaterial calcined at 750 °C provides the best anti-microbial activity against Escherichia coli, Salmonella typhimurium, Shigella flexneri (gram-negative), Bacillus subtilis and Bacillus megaterium (gram-positive) bacterial strain at 300 µg/mL concentration. The nanomaterial with calcination temperatures of 300 °C and 450 °C provided better antioxidant properties.

Zinc oxide nano-flowers improve the growth and propagation of mulberry cuttings grown under different irrigation regimes by mitigating drought-related complications and enhancing zinc uptake.

Silkworm larvae mainly consume mulberry leaves; therefore, mulberry cultivation is important for the production of raw silk. Drought stress and micronutrient deficiency (Zn) are known to affect the propagation of mulberry cuttings. In this purview, the current investigation attempted to inspect the efficacy of different concentrations of zinc oxide nano-flower (ZnNFs) applied through both soil admixture and foliar spray on the propagation of mulberry cuttings grown under deficit irrigation regimes. The overall results demonstrated that the ZnNF-treated plant cuttings were well-adapted to drought stress and performed better in comparison to the control set. Out of the tested concentrations - ZnNF-10 (applied as 10 mg/kg soil and 10 ppm as foliar spray thrice) was found to be optimum, showing relatively better initial root establishment, the emergence of leaves, and survival and sprouting percentage. Further studies also confirmed an improvement in the accumulation of photosynthetic pigments, carbohydrates, and protein content even under extreme drought conditions. Most importantly, the ZnNF-10 treatment contributed to ROS detoxification and cell membrane protection by enhancing the pool of antioxidant enzymes. The study further demonstrated that ZnNF-10 application enhanced zinc content by 147.50%, 179.49%, and 171.99% in root, shoot, and leaves of the treated cuttings; thereby, improving the bioaccumulation factor of the plant parts. All of these interactive phenomena led to an increment in shoot height, biomass, leaf area, and leaf number of cuttings. These findings, therefore, indicated that ZnNFs can be developed as a promising nano-fertilizer for mulberry growth facilitating Zn uptake and mitigation of drought-induced complications.

Endophytic fungi mediates production of bioactive secondary metabolites via modulation of genes involved in key metabolic pathways and their contribution in different biotechnological sector.

Endophytic fungi stimulate the production of an enormous number of bioactive metabolites in medicinal plants and affect the different steps of biosynthetic pathways of these secondary metabolites. Endophytic fungi possess a number of biosynthetic gene clusters that possess genes for various enzymes, transcription factors, etc., in their genome responsible for the production of secondary metabolites. Additionally, endophytic fungi also modulate the expression of various genes responsible for the synthesis of key enzymes involved in metabolic pathways of such as HMGR, DXR, etc. involved in the production of a large number of phenolic compounds as well as regulate the expression of genes involved in the production of alkaloids and terpenoids in different plants. This review aims to provide a comprehensive overview of gene expression related to endophytes and their impact on metabolic pathways. Additionally, this review will emphasize the studies done to isolate these secondary metabolites from endophytic fungi in large quantities and assess their bioactivity. Due to ease in synthesis of secondary metabolites and their huge application in the medical industry, these bioactive metabolites are now being extracted from strains of these endophytic fungi commercially. Apart from their application in the pharmaceutical industry, most of these metabolites extracted from endophytic fungi also possess plant growth-promoting ability, bioremediation potential, novel bio control agents, sources of anti-oxidants, etc. The review will comprehensively shed a light on the biotechnological application of these fungal metabolites at the industrial level.

Engineered nanoparticles in plant growth: Phytotoxicity concerns and the strategies for their attenuation.

In the agricultural sector, the use of engineered nanoparticles (ENPs) has been acclaimed as the next big thing for sustaining and increasing crop productivity. A vast amount of literature is available regarding the growth-promoting attributes of different ENPs. In this context, it has been emphasized that the ENPs can bolster vegetative growth, leaf development, and seed setting and also help in mitigating the effects of abiotic and biotic stresses. At the same time, there have been a lot of speculations and concerns regarding the phytotoxicity of ENPs off-late. In this connection, many research articles have presented the negative effects of ENPs on plant systems. These studies have highlighted that almost all the ENPs impart a certain degree of phytotoxicity in terms of reduction in growth, biomass, impairment of photosynthesis, oxidative status of plant cells, etc. Mostly, the ENPs based on metal or metal oxides (Cd, Cr, Pb, Ag, Ce, etc.) and nonmetals (C) that are introduced into the environment are known to incite inhibitory effects. However, the phytotoxicity of ENPs are known to be determined mostly by the chemical nature of the element, size, surface charge, coating molecules, and abiotic factors like pH and light. This review article, therefore, elucidates the phytotoxic properties of different ENPs and the plant responses induced at the molecular level subjected to nanoparticle exposure. Moreover, the article highlights the probable strategies that may be adopted for the suppression of the phytotoxicity of ENPs to ensure the safe and sustainable application of ENPs in crop fields.

Optimization of starch extraction from Amorphophallus paeoniifolius corms using response surface methodology (RSM) and artificial neural network (ANN) for improving yield with tenable chemical attributes.

The development of the extraction process for improving the starch yield from unconventional plants is emerging as a topic of interest. In this respect, the present work aimed to optimize the starch extraction from the corms of elephant foot yam (Amorphophallus paeoniifolius) with the help of response surface methodology (RSM) and artificial neural network (ANN). The RSM model performed better than the ANN in predicting the starch yield with higher precision. In this connection, this study for the first time reports the significant improvement of starch yield from A. paeoniifolius (51.76 g/100 g of the corm dry weight). The extracted starch samples based on yield - high (APHS), medium (APMS), and low (APLS) exhibited a variable granule size (7.17-14.14 µm) along with low ash content, moisture content, protein, and free amino acid indicating purity and desirability. The FTIR analysis also confirmed the chemical composition and purity of the starch samples. Moreover, the XRD analysis showed the prevalence of C-type starch (2θ = 14.303°). Based on other physicochemical, biochemical, functional, and pasting properties, the three starch samples showed more or less similar characteristics thereby indicating the sustentation of beneficial attributes of starch molecules irrespective of the variation in extraction parameters.

Exogenous nano-silicon application improves ion homeostasis, osmolyte accumulation and palliates oxidative stress in Lens culinaris under NaCl stress.

Lentil is one of the highly nutritious legumes but is highly susceptible to salinity stress. Silicon has been known to reduce the effect of various environmental stresses including salinity. Moreover, silicon when applied in its nano-form is expected to augment the beneficial attributes of silicon. However, very little is known regarding the prospect of nano-silicon (nSi) application for alleviating the effect of salinity stress in non-silicified plants like lentil. In this study, the primary objective was to evaluate the efficacy of nSi in the alleviation of NaCl stress during germination and early vegetative stages. In this context, different concentrations of nSi (0, 1, 5, 10 g L-1) was applied along with four different concentrations of NaCl (0, 100, 200, 300 mM). The results indicated the uptake of nSi which was confirmed by the better accumulation of silica in the plant tissues. Most importantly, the enhanced accumulation of silica increased the K+/Na+ ratio of the NaCl-stressed seedlings. Moreover, nSi efficiently improved germination, growth, photosynthetic pigments, and osmotic balance. On the other hand, the relatively reduced activities of antioxidative enzymes were surmounted by the higher activity of non-enzymatic antioxidants which mainly scavenged the increased ROS. Reduced ROS accumulation in return ensured better membrane integrity and reduced electrolyte leakage up on nSi application. Therefore, it can be concluded that the application of nSi (more specifically at 10 g L-1) facilitated the uptake of silica and improved the K+/Na+ ratio to reclaim the growth and physiological status of NaCl-stressed seedlings.

Sugar-terminated carbon-nanodots stimulate osmolyte accumulation and ROS detoxification for the alleviation of salinity stress in Vigna radiata.

In recent times, nanotechnology has emerged as an efficient tool to manage the adverse effect of environmental stresses on plants. In this connection, carbon-nanodots (CNDs) have been reported to ameliorate the negative impacts of salinity stress. Further, surface modification of CNDs is believed to augment their stress-alleviating potential, however, very little has been known about the potential of surface-functionalized CNDs. In this purview, two sugar (trehalose and glucose) terminated CNDs (CNPT and CNPG) have been synthesized and assessed for their stress-alleviating effects on Vigna radiata (a salt-sensitive legume) seedlings subjected to different concentrations of NaCl (0, 50, and 100 mM). The synthesized CNDs (CNPT and CNPG) exhibited a hydrodynamic size of 20-40 nm and zeta potential of up to - 22 mV with a 5-10 nm core. These water-soluble nanomaterials exhibited characteristic fluorescence emission properties viz. orange and greenish-yellow for CNPT and CNPG respectively. The successful functionalization of the sugar molecules on the CND cores was further confirmed using FTIR, XRD, and AFM. The results indicated that the application of both the CNDs improved seed germination, growth, pigment content, ionic and osmotic balance, and most importantly, the antioxidant defense which decreased ROS accumulation. At the same time, CNPT and CNPG exhibited no toxicity in the Allium cepa root tip bioassay. Therefore, it can be concluded that sugar-terminated CNDs improved the plant responses to salinity stress by facilitating sugar uptake to the aerial part of the seedlings.

Effect of plastic pollution on freshwater flora: A meta-analysis approach to elucidate the factors influencing plant growth and biochemical markers.

The deterioration in the water quality of urban water bodies through plastic contamination is emerging as a matter of serious concern. Microplastics (MPs) and nanoplastics (NPs) both affect the growth and productivity of aquatic flora. However, there have been a lot of variations in the reported studies which calls for revisiting the results with an analytical approach. Therefore, this study was designed to systematically evaluate the publications based on PRISMA (2020) guidelines. In this connection, 43 eligible articles were selected for meta-analysis followed by subgroup analysis to determine the impact of size, concentration, plastic polymers, and effect of plant classes on several physiological and biochemical parameters (growth, chlorophyll-a, carotenoids, protein, and antioxidant enzymes). The results indicated that the higher concentrations of plastics negatively affected the growth, and also enhanced the protein content and antioxidative enzyme activity. While, NPs were found to impart an inhibitory effect on pigment contents, along with a significant increase in protein content and antioxidative enzyme activity. Among the plastic polymers, dibutyl phthalate (DBP) showed a comparatively higher effect on growth, whereas the photosynthetic pigments were disrupted to a greater extent in the presence of polyvinyl chloride (PVC) plastics. Moreover, the growth parameters under plastic exposure were affected in the algal members to a greater extent in comparison to the other plant groups. Lastly, several plants like Komvophoron, Elodea, Myriophyllum, Nostoc, Raphidocelis, Scenedesmus, Utricularia, Dunaliella, and Lemna appeared to be more tolerant than others (Tolerance Index ≥ 0.8), showing a significantly minimal effect on growth inhibition.

An evolutionary divergent thermodynamic brake in ZAP-70 fine-tunes the kinetic proofreading in T cells.

T cell signaling starts with assembling several tyrosine kinases and adapter proteins to the T cell receptor (TCR), following the antigen binding to the TCR. The stability of the TCR-antigen complex and the delay between the recruitment and activation of each kinase determines the T cell response. Integration of such delays constitutes a kinetic proofreading mechanism to regulate T cell response to the antigen binding. However, the mechanism of these delays is not fully understood. Combining biochemical experiments and kinetic modeling, here we report a thermodynamic brake in the regulatory module of the tyrosine kinase ZAP-70, which determines the ligand selectivity, and may delay the ZAP-70 activation upon antigen binding to TCR. The regulatory module of ZAP-70 comprises of a tandem SH2 domain that binds to its ligand, doubly-phosphorylated ITAM peptide (ITAM-Y2P), in two kinetic steps: a fast step and a slow step. We show the initial encounter complex formation between the ITAM-Y2P and tandem SH2 domain follows a fast-kinetic step, whereas the conformational transition to the holo-state follows a slow-kinetic step. We further observed a thermodynamic penalty imposed during the second phosphate-binding event reduces the rate of structural transition to the holo-state. Phylogenetic analysis revealed the evolution of the thermodynamic brake coincides with the divergence of the adaptive immune system to the cell-mediated and humoral responses. In addition, the paralogous kinase Syk expressed in B cells does not possess such a functional thermodynamic brake, which may explain the higher basal activation and lack of ligand selectivity in Syk.

Bisphenol-A incite dose-dependent dissimilitude in the growth pattern, physiology, oxidative status, and metabolite profile of Azolla filiculoides.

Bisphenol-A (BPA) is a ubiquitous environmental pollutant affecting the growth and development of aquatic macrophytes. The present study was designed to evaluate the toxic effect of BPA on Azolla filiculoides. The plants were exposed to different concentrations of BPA and the effect was evaluated in terms of plant growth, physiological and oxidative status, responses of the antioxidative system, and changes in key metabolites. The results have shown that BPA (≥ 20 mg L-1) incites a significant reduction in frond number, frond surface area, and growth rate of the plants along with severe frond damage, membrane peroxidation, and electrolyte leakage. Moreover, at higher concentrations, a significant reduction in the content of chlorophylls and carotenoids was observed, which was further amplified with the duration of treatments. Furthermore, excessive generation of O2•- and H2O2 invoked the antioxidative machinery under BPA exposure. However, sufficient activity of the antioxidative enzymes was observed in plants treated with ≤ 10 mg L-1 of BPA. The untargeted metabolome profile revealed modulation of 29 metabolites including amino acids, sugar alcohols, organic acids, and phenolics in response to BPA. An increased amount of asparagine, lysine, serine, tryptophan, tyrosine, and valine after 3 days of BPA exposure indicates their role in providing better stress tolerance. Therefore, the experimental findings suggest that A. filiculoides responds differently to BPA exposure. Higher BPA concentrations (≥ 20 mg L-1) documented a greater impact in terms of plant physiology and metabolism whereas, the effect was minimal at lower concentrations (≤ 10 mg L-1).