RESUMO
C3 complement component and its C3d subunit were isolated from the IY-1 Cohn's fraction, which is the waste of industrially produced albumin and immunoglobulins. The first step was the fractionation of precipitate IY-1 by polyethylene glycol (PEG) 4000 to a final concentration of 16% PEG. The precipitate formed was separated by centrifugation. The supernatant contained the C3d subunit of C3, and the redissolved 16% PEG precipitate contained the C3 component. Then the supernatant and the dissolved precipitate were subjected to anion-exchange chromatography on DEAE-Toyopearl 650 M. In the last step fractions containing C3 and C3d concentrated by ultrafiltration were chromatographed on Sephacryl S-200.
Assuntos
Proteínas Sanguíneas/química , Complemento C3/isolamento & purificação , Complemento C3d/isolamento & purificação , Cromatografia em Gel , Cromatografia por Troca Iônica , Humanos , Imunoeletroforese , Espectrofotometria UltravioletaRESUMO
Azathioprine immunosuppressive therapy prolongs remissions and stimulates residual beta-cell function, suppresses insulin antibody production, reduces the activity of the complement and CH50 components, reduces initially increased cellular immunity parameters (total T and B cell counts, T helper to T inductor ratio, and the count of DR carrier cells) in patients with newly detected insulin-dependent diabetes mellitus; this makes this drug effective at the first stages of the disease. When selecting patients for immunosuppressive therapy the following immunity parameters should be examined: complement status, total counts of T and B lymphocytes, T-helper-inductor/T-suppressor-cytotoxic immunoregulation index, DR carrier cell counts. Reduced levels thereof are a contraindication against immunosuppressant therapy. Male patients with insulin-dependent diabetes mellitus debut at the age of over 25 are particularly susceptible to immunosuppressive therapy with azathioprine.
Assuntos
Azatioprina/uso terapêutico , Diabetes Mellitus Tipo 1/tratamento farmacológico , Adolescente , Adulto , Formação de Anticorpos/efeitos dos fármacos , Linfócitos B/efeitos dos fármacos , Peptídeo C/metabolismo , Metabolismo dos Carboidratos , Proteínas do Sistema Complemento/efeitos dos fármacos , Diabetes Mellitus Tipo 1/imunologia , Feminino , Humanos , Imunidade Celular/efeitos dos fármacos , Contagem de Leucócitos/efeitos dos fármacos , Masculino , Indução de Remissão/métodos , Caracteres Sexuais , Linfócitos T/efeitos dos fármacos , Fatores de TempoRESUMO
Serum levels of circulating immune complexes (CIC) and those in the liquor were studied in patients with spinal trauma using enzyme immunoassay. CIC and clinical--laboratory findings characterizing the activity of the inflammation were consistent. Assessment of CIC factor in spinal trauma seems clinically valuable as it is involved in inflammation and tissue damage suggesting a prognosis of CIC-related pathology.
