The influence of light intensities and micropollutants on the removal of total coliforms and E. coli from wastewater in a flat-panel photobioreactor.

The presence of micropollutants and pathogens in sanitary wastewater and surface water is a growing concern that impacts public health, environmental balance and the maintenance of water supply services. To improve sanitary wastewater treatment, it is necessary to develop and improve sustainable technologies. Among the available options, microalgae-based systems stand out for their efficiency and generation of value-added byproducts. To study the impact of luminosity and the presence of micropollutants (13 selected) on the removal of E. coli and total coliforms from real anaerobically treated wastewater, a pilot flat-panel photobioreactor (50 L) was operated in batch mode in a tropical climate region. This is the first study to evaluate whether micropollutants interfere with coliform groups, considering a microalgae-based system and an experiment in a tropical climate region. E. coli had better removal (from 104 to 101 CFU 100 mL-1) than did total coliforms (from 104 to 103 CFU 100 mL-1). The removal of E. coli was more strongly linked to luminosity and temperature, while the removal of total coliforms was influenced by the presence of the selected micropollutants.

Evaluation of the effect of the feeding regime on the removal of metals and pathogens in microalgae-bacterial systems.

Microalgae-bacteria systems are used for the treatment of effluents, using a technology that has stood out with excellent results, as reported in the literature. However, investigating these systems in more depth can improve our understanding of the removal mechanisms for a wide range of existing and emerging pollutants and help improve the guidelines for design and operation, in order to improve the treatment efficiency as well as biomass productivity. This work studied the impact of the feeding regime on the removal of metals and pathogens from primary domestic wastewater in high rate algal ponds (HRAPs). For this, one reactor was fed continuously (HRAP1) while two reactors were fed in semi-continuous mode, during 12 h day-1 (HRAP2) and 0.1 h day-1 (HRAP3). Although removal efficiencies of 82 ± 5% for Mn and 90% for E. coli were reached in the semi-continuously fed reactors, there was no significant difference between the conditions studied. On the other hand, for biomass productivity, the semi-continuous feeding regime was more advantageous with a growth of ≈ 22 mg L-1 day-1.

Influence of the hydraulic retention time on the removal of emerging contaminants in an anoxic-aerobic algal-bacterial photobioreactor coupled with anaerobic digestion.

This work evaluated, for the first time, the performance of an integral microalgae-based domestic wastewater treatment system composed of an anoxic reactor and an aerobic photobioreactor, coupled with an anaerobic digester for converting the produced algal-bacterial biomass into biogas, with regards to the removal of 16 contaminants of emerging concern (CECs): penicillin G, tetracycline, enrofloxacin, ciprofloxacin, sulfamethoxazole, tylosin, trimethoprim, dexamethasone, ibuprofen, naproxen, acetaminophen, diclofenac, progesterone, carbamazepine, triclosan and propylparaben. The influence of the hydraulic retention time (HRT) in the anoxic-aerobic bioreactors (4 and 2.5 days) and in the anaerobic digester (30 and 10 days) on the fate of these CECs was investigated. The most biodegradable contaminants (removal efficiency >80% regardless of HRT) were tetracycline, ciprofloxacin, sulfamethoxazole, tylosin, trimethoprim, dexamethasone, ibuprofen, naproxen, acetaminophen and propylparaben (degraded predominantly in the anoxic-aerobic bioreactors), and tetracycline, sulfamethoxazole, tylosin, trimethoprim and naproxen (degraded predominantly in the anaerobic reactor). The anoxic-aerobic bioreactors provided removal of at least 48% for all CECs tested. The most recalcitrant contaminants in the anaerobic reactor, which were not removed at any of the HRT tested, were enrofloxacin, ciprofloxacin, progesterone and propylparaben.

Integration of algae-based sewage treatment with anaerobic digestion of the bacterial-algal biomass and biogas upgrading.

The domestic sewage treatment performance of an integrated anoxic-aerobic photobioreactor with biomass settling and recycling, coupled with anaerobic digestion of the produced bacterial-algal biomass and biogas upgrading in the photobioreactor was investigated. Hydraulic retention time in the photobioreactor initially was 4 days (stage I and II) and then reduced to 2.5 days (stage III). The integrated system supported high total organic carbon removals of 98.9 ± 1.1% regardless of the operational stage. A high total nitrogen removal of 90.8 ± 8.0% was recorded in the integrated system during the three operational stages, while total phosphorus removals accounted for 68.4 ± 20.1%, 68.3 ± 20.8% and 53.4 ± 25.0% in stages I, II and III, respectively. Biogas upgrading in the absorption column exhibited maximum removals of CO2 and H2S of 74.7 ± 3.0% and 99.0 ± 2.8%, respectively. Biomass settling and recycling resulted in overall improvement of biomass settleability.

Chlorella vulgaris growth on anaerobically digested sugarcane vinasse: influence of turbidity.

This paper shows the influence of turbidity (in Nephelometric Turbidity Units - NTU), chemical oxygen demand (COD) and aeration (CO2 supply) on the productivity and growth rate and lipid content of microalgae (a mixed culture predominantly composed of Chlorella vulgaris), using anaerobically digested vinasse as a culture medium. The microalgae can be cultivated in anaerobically digested vinasse, at turbidity and chemical oxygen demand of 690 NTU and 2.5 gCOD L -1, respectively, according to the modified Gompertz model, and removal of turbidity by filtration did not influence the microalgae productivity (≈ 77 mg L1 d1). Furthermore, aeration increased the productivity up to 139 mg L1 d1, with a biomass dry weight of 2.7 g L-1. Finally, a maximum lipid content of 265 mg L -1 was obtained, while a nitrogen removal of 98% was recorded for all conditions. Thus, the combination of anaerobic digestion followed by the use of the digestate for the cultivation of microalgae may be an efficient way to treat large quantities of this residue, in turn yielding large amounts of microalgae biomass, which can be transformed into fertilizer and biofuel.

Surfactant removal and biomass production in a microalgal-bacterial process: effect of feeding regime.

The influence of the feeding regime on surfactant and nutrient removal and biomass production was evaluated in three high rate algal ponds for primary domestic wastewater treatment. Feeding times of 24, 12 and 0.1 h d-1 were studied in each reactor at a similar hydraulic retention time of 7.0 days and organic load of 2.3 mg m-2 d-1. Semi-continuous feeding at 12 and 0.1 h d-1 showed better microalgal biomass production (0.21-0.23 g L-1) and nutrient removal, including nitrogen (74-76%) and phosphorus (80-86%), when compared to biomass production (0.13 g L-1) and nitrogen (69%) and phosphorus (46%) removals obtained at continuous feeding (24 h d-1). Additionally, the removal efficiency of surfactant in the three reactors ranged between 90 and 97%, where the best result was obtained at 0.1 h d-1, resulting in surfactant concentrations in the treated effluent (0.3 mg L-1) below the maximum freshwater discharge limits.

The effect of CO2 addition and hydraulic retention time on pathogens removal in HRAPs.

The influence of CO2 addition and hydraulic retention time (5 and 7 days) on removal of Pseudomonas aeruginosa, Clostridium perfringens, Staphylococcus sp., Enterococcus sp., and Escherichia coli was evaluated in a system with three parallel 21 L high rate algal ponds. Both the addition of CO2 and an increase in HRT had no significant influence on bacterial removal, but bacterial removal was higher than found in previous studies. The removal was 3.4-3.8, 2.5-3.7, 2.6-3.1, 2.2-2.6 and 1.3-1.7 units log for P. aeruginosa, E. coli, Enterococcus sp., C. perfringens, and for Staphylococcus sp., respectively. Although CO2 addition did not increase disinfection, it did significantly increase biomass productivity (by ≈60%) and settleability (by ≈350%). Additionally, even at the lower 5-day hydraulic retention time, CO2 addition improves removal of chemical oxygen demand (COD), total organic carbon (TOC), total organic nitrogen and phosphorus by 97, 91, 12 and 50%, respectively.