New insights into plant salt acclimation: the roles of vesicle trafficking and reactive oxygen species signalling in mitochondria and the endomembrane system.

In this study, we investigated the cellular and molecular mechanisms that regulate salt acclimation. The main objective was to obtain new insights into the molecular mechanisms that control salt acclimation. Therefore, we carried out a multidisciplinary study using proteomic, transcriptomic, subcellular and physiological techniques. We obtained a Nicotiana tabacum BY-2 cell line acclimated to be grown at 258 mM NaCl as a model for this study. The proteomic and transcriptomic data indicate that the molecular response to stress (chaperones, defence proteins, etc.) is highly induced in these salt-acclimated cells. The subcellular results show that salt induces sodium compartmentalization in the cell vacuoles and seems to be mediated by vesicle trafficking in tobacco salt-acclimated cells. Our results demonstrate that abscisic acid (ABA) and proline metabolism are crucial in the cellular signalling of salt acclimation, probably regulating reactive oxygen species (ROS) production in the mitochondria. ROS may act as a retrograde signal, regulating the cell response. The network of endoplasmic reticulum and Golgi apparatus is highly altered in salt-acclimated cells. The molecular and subcellular analysis suggests that the unfolded protein response is induced in salt-acclimated cells. Finally, we propose that this mechanism may mediate cell death in salt-acclimated cells.

Systems involved in K+ uptake from diluted solutions in pepper plants as revealed by the use of specific inhibitors.

Here, the contribution of the HAK1 transporter, the AKT1 channel and a putative AtCHX13 homolog to K(+) uptake in the high-affinity range of concentrations in pepper plants was examined. The limited development of molecular tools in pepper plants precluded a reverse genetics study in this species. By contrast, in the model plant Arabidopsis thaliana, these type of studies have shown that NH(4)(+) and Ba(2+) may be used as specific inhibitors of the two K(+) uptake systems to dissect their contribution in species in which, as in pepper, specific mutant lines are not available. By using these inhibitors together with Na(+) and Cs(+), the relative contributions of CaHAK1, CaAKT1 and a putative AtCHX13 homolog to K(+) acquisition from diluted solutions under different regimens of K(+) supply were studied. The results showed that, in plants completely starved of K(+), the gene encoding CaHAK1 was highly expressed and this system is a major contributor to K(+) uptake. However, K(+) concentrations as low as 50µM reduced CaHAK1 expression and the CaAKT1 channel came into play, participating together with CaHAK1 in K(+) absorption. The contribution of a putative AtCHX13 homolog seemed to be low under this low K(+) supply, but it cannot be ruled out that at higher K(+) concentrations this system participates in K(+) uptake. Studies of this type allow extension of the tools developed in model plants to understand nutrition in important crops.