[Sex identification of the rainbow trout Oncorhynchus mykiss by polymerase chain reaction].

A polymerase chain reaction-based test for rapid sex identification of the rainbow trout Oncorhynchus mykiss has been elaborated. Using the data deposited with the NCBI genome bank, nucleotide sequences of the specific sex-linked locus of salmonid species have been analyzed and the oligonucleotide primers have been selected. The length of the amplification products is 800 nucleotide pairs. Amplification specificity has been tested by nucleotide analysis of the amplicon sequences. All PCR products match the Y chromosome region housing the corresponding specific locus. A comparative analysis of the nucleotide sequences of the fragments carrying the rainbow trout (and other salmonid species) sex-linked marker demonstrates a high degree of identity, amounting to 95-99%. The maximal identity between the sequences (99%) is observed when analyzing representatives of the same genus, for example, Oncorhynchus. Thus, the sex is detectable by a simple PCR test, thereby allowing the male revertants emerging via hormonal sex reversion to be identified. This test is a rapid diagnostic method, since it requires only 1 day for testing and informing the fish farm on its results. Results of abdomen opening of rainbow trout revertants and examination of their hormonal status are also described.

Using C60 fullerenes for photodynamic inactivation of mosquito iridescent viruses.

This article describes the photodynamic inactivation of mosquito iridescent virus (MIV) Aedes flavescens in the presence of water-soluble C(60) fullerenes. It has been observed that the photodynamic inactivation of MIV for about 1 h reduces the infectious titre of the virus in large wax-moth larvae Galleria mellonella to 4.5 lg ID(50)/mL. The influence of the C(60) concentration on its anti-viral activity was tested in the concentration range from 1 to 0.001 mg/mL. It has been found that C(60) is able to inactivate the iridovirus even in low concentrations. Consequently, the findings of this work suggest that photoexcited C(60) fullerenes can be successfully used for the inactivation of iridoviruses in biological systems.