Study ofthe comparative efficacy of the phytotherapeutic complex against nematodes of mice

The purpose of the research is to studythe efficacy of phytotherapeutic, and homeopathic drugs. Phytotherapeutic drugs have been widely used in parasitology for many years. Also, homeopathic drugs such as Cina have anti-helminthicproperties. The possibility of using phytotherapeutic and high dilutiondrugs during the treatment of nematodedisease has been studied. Two experimental parasitological disease models (aspiculuriosis and trichinellosis) were used.Trichinellosisis a common model of nematodesinmice [1,2].Aspiculuris tetrapteraa pinworm of mice is an important parasite in institutions with mice colonies for both research and teaching purposes. The infection is generally asymptomatic. This study aimed to assess the protective efficiency of a homeopathic drug such as Cina C6cH, sphagnum,and its complex against experimental trichinosis and aspiculuriosis. Methods:An assay was carried out on 80 white outbred mice. These were divided into 6 groups of ten mice each. Groups1 and 4 had sphagnum q.s. per os; groups2 and 5 -desoldering dissolved in water Cina C6cH one time a day per os, the groups3 and 6 received complex sphagnum+Cina C6cHone time a day per os. Groups1-3 were inoculated with a dose of 80 ± 5 units of T. spiralislarva per mouse, groups 4-6 had spontaneous aspiculuriosis, and group 7 had T. spiralis, group 8 had Aspiculuris tetraptera. After 60 days of trichinellosis incubation, process the mice were euthanized and dissected for evaluation.Results:Maximum protection was obtained in the group of mice 3: T. spiralisdetected larvae in animals was 650.5±25.1 larva/animal. Group N2 (Cina C6cH) presented 2840.5±183.3 larva/animal. This was less than the control group (4485±430.6 larva/mouse). Also,groups 4-6 of mice with aspiculuriosis showed 75%, 80%,and 86% efficacy against nematodes.

Reprint of: The retention and concentration of glycogen in Trichinella nativа in the winter-spring period.

The metabolism of Trichinella spp. is primarily anoxybiotic in nature. Their main energy source is glycogen, which is stored in the stichocites at the muscular stage of the larval development. When subject to tow temperatures the Trichinella larvae consume glycogen and neutral fats to provide for basal metabolism until the energy supplies reach the critical level. The present study establishes the glycogen concentration as well the invasive activity of T. nativа when affected by low temperatures in natural conditions. The carcasses of infected laboratory rats were placed in containers beneath the snow cover, in the natural conditions of a game husbandry in Central Russia. The viability, invasive capacity and the glycogen level were monitored in the Trichinella larvae monthly. The invasive capacity of Trichinella larvae was established based on the presence of the larvae in the muscular tissue of laboratory mice after the peroral administration of the helminth larvae. On the 45 day of the experiment, the mice were euthanized by cervical dislocation, and if the Trichinella larvae could be discovered in the muscular tissue with the help of the trichinelloscopic compression method, the invasive capacity of the Trichinella larvae was viewed as positive. To establish the quantitative value of glycogen content in Trichinella larvae a modified method was used. In order to measure the glycogen level in the T. nativa larvae isolated by fermentation larvae were counted in one drop of the suspended sedimentation in the Migacheva-Kotelnikov chamber. To establish the quantitative value of glycogen content in Trichinella larvae a method based on the treatment of glycogen with iodine, optical density measurement with a refractometer MКMФ-02 was used. For the purpose of measuring the concentration of glycogen in Trichinella larvae in the suspended sedimentation a calibration curve was used. The studies showed that the viability indicator of the Trichinella larvae which had been preserved in natural conditions in the four months of the winter-spring period, in the muscular tissue of laboratory rats remained high (over 90 %). The glycogen concentration in one helminth larva was 0.041 µg in January, 0.033 µg in February, 0.015 µg in April. The invasive capability of the preserved Trichinella larvae was considerably reduced to 33.3 %. In the winter period, under temperatures below 0 °C, a decrease in the glycogen concentration in the Trichinella larvae was observed.

The retention and concentration of glycogen in Trichinella nativа in the winter-spring period.

The metabolism of Trichinella spp. is primarily anoxybiotic in nature. Their main energy source is glycogen, which is stored in the stichocites at the muscular stage of the larval development. When subject to tow temperatures the Trichinella larvae consume glycogen and neutral fats to provide for basal metabolism until the energy supplies reach the critical level. The present study establishes the glycogen concentration as well the invasive activity of T. nativа when affected by low temperatures in natural conditions. The carcasses of infected laboratory rats were placed in containers beneath the snow cover, in the natural conditions of a game husbandry in Central Russia. The viability, invasive capacity and the glycogen level were monitored in the Trichinella larvae monthly. The invasive capacity of Trichinella larvae was established based on the presence of the larvae in the muscular tissue of laboratory mice after the peroral administration of the helminth larvae. On the 45 day of the experiment, the mice were euthanized by cervical dislocation, and if the Trichinella larvae could be discovered in the muscular tissue with the help of the trichinelloscopic compression method, the invasive capacity of the Trichinella larvae was viewed as positive. To establish the quantitative value of glycogen content in Trichinella larvae a modified method was used. In order to measure the glycogen level in the T. nativa larvae isolated by fermentation larvae were counted in one drop of the suspended sedimentation in the Migacheva-Kotelnikov chamber. To establish the quantitative value of glycogen content in Trichinella larvae a method based on the treatment of glycogen with iodine, optical density measurement with a refractometer MКMФ-02 was used. For the purpose of measuring the concentration of glycogen in Trichinella larvae in the suspended sedimentation a calibration curve was used. The studies showed that the viability indicator of the Trichinella larvae which had been preserved in natural conditions in the four months of the winter-spring period, in the muscular tissue of laboratory rats remained high (over 90 %). The glycogen concentration in one helminth larva was 0.041 µg in January, 0.033 µg in February, 0.015 µg in April. The invasive capability of the preserved Trichinella larvae was considerably reduced to 33.3 %. In the winter period, under temperatures below 0 °C, a decrease in the glycogen concentration in the Trichinella larvae was observed.