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1.
Phys Chem Chem Phys ; 24(48): 30062, 2022 Dec 14.
Artigo em Inglês | MEDLINE | ID: mdl-36484314

RESUMO

Correction for 'Zinc(II) hydration in aqueous solution. A Raman spectroscopic investigation and an ab initio molecular orbital study' by Wolfram W. Rudolph et al., Phys. Chem. Chem. Phys., 1999, 1, 4583-4593, https://doi.org/10.1039/A904051J.

2.
Microorganisms ; 10(9)2022 Aug 31.
Artigo em Inglês | MEDLINE | ID: mdl-36144369

RESUMO

Recent publications indicate that A. radioresistens can cause infections in humans, even though it is rarely reported in routine diagnostics. However, the fact that it is infrequently detected may be explained by the misidentification of the species by conventional methods. It is also likely that A. radioresistens is not considered clinically relevant and therefore not consistently included in diagnostic results. To elucidate the medical significance of this probably clinically underestimated bacterial species, we created a well-documented reference strain collection of 21 strains collected in routine diagnostics. For further analysis of A. radioresistens, it is essential to know which methods can be used to achieve a trustworthy identification. We, therefore, compared three methods widely used in routine diagnostics (MALDI-TOF MS, VITEK 2, and sequencing of housekeeping genes) in terms of secure and reliable identification of A. radioresistens. As reference methods, whole genome-based approaches were applied. VITEK 2 led to misidentification for four strains. However, MALDI-TOF MS and sequencing of housekeeping genes led to reliable and robust identifications.

3.
Appl Spectrosc ; 75(4): 395-411, 2021 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-33393351

RESUMO

Qualitative and quantitative Raman and infrared measurements on sodium nitrate (NaNO3) solutions have been carried out over a wide concentration range (5.56 × 10-6-7.946 mol/L) in water and heavy water. The Raman spectra were measured from 4000 cm-1 to low wavenumbers at 45 cm-1. Band fit analysis on the profile of the 1047 cm-1 band, ν1(a1') NO3- measured at high resolution at 0.90 cm-1 produced a small contribution at 1027 cm-1 of the isotopomer N16O218O(aq). The effect of solute concentration on the Raman and infrared bands has been systematically recorded. Extrapolation of the experimental data resulted in values for all the nitrate bands of the "free", i.e., fully hydrated NO3-(aq). However, even in dilute solutions, the vibrational symmetry of the hydrated NO3-(aq) is broken and the antisymmetric N-O stretch, which is degenerate for the isolated anion, is split by 56 cm-1. At concentrations >2.5 mol/L, direct contact between Na+ and NO3- was observed and accompanied by large band parameter changes. DFT calculations on NO3-(H2O)n (n = 1-3) led to optimized geometries and vibrational frequencies which reproduced the measured ones within an accuracy of 1%. A hydrated gas phase species Na+(H2O)10NO3- was optimized resulting in the geometry and symmetry of the nitrate, which is bound in an antisymmetric bidentate fashion with the nitrate possessing C1. The ν1 Na+(OH2) breathing mode in aqueous solution appears at 189 cm-1, whereas in heavy water, ν1 Na+(OD2) is shifted to 175.6 cm-1 due to the isotope effect. DFT calculations on hydrated Na+(OH2)n gas phase clusters provided realistic Na+ hydrate structures with n = 4 and 5, which resembled the measured frequency of ν1 Na+ OH2 mode quite well. Quantitative Raman analysis employing the symmetric stretching band, ν1(a1') NO3-, has been carried out down to concentrations as low as 5.56 × 10-6 mol/L. The in-plane deformation mode ν4(e') in the Raman scattering at higher concentrations has been used as an indicator band for directly coordinated NO3-.

4.
Microb Pathog ; 134: 103576, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31185244

RESUMO

The bacterial species Roseomonas mucosa is pathogenic in humans, and although it is rarely detected during routine diagnostics, it is becoming increasingly important clinically. For a long time, R. mucosa was regarded as a classic environmental bacterium. Recent studies, however, revealed that it is part of the physiological human skin flora and mainly affects immunocompromised patients. Furthermore, the use of catheter systems may increase the risk of contracting R. mucosa infections. The bacterium has been linked to severe infections, such as bacteraemia, osteomyelitis and cellulitis. Therefore, it is important to discern the best method of identifying R. mucosa in routine laboratory testing. To facilitate this testing, we compared three suitable methods for routine bacterial identification in the laboratory: VITEK 2, MALDI-TOF MS and 16S rRNA gene sequencing. Additionally, we conducted whole-genome sequencing (WGS) and calculated the average nucleotide identity (ANI). ANI is seen as the gold standard of strain identification; therefore, we decided to use it as a reference method. Both MALDI-TOF MS and 16S rRNA gene sequencing confidently identified the species. However, when using the VITEK 2 technique, isolates were misidentified as Roseomonas gilardii, Rhizobium radiobacter, or Sphingomonas paucimobilis. When conducting WGS and determining the ANI, it became obvious that one isolate belonged to the species R. gilardii rather than R. mucosa. Therefore (although not yet applicable in routine diagnostics), we suggest that WGS is presently the most appropriate technique to reliably identify Roseomonas mucosa. However, after expanding the Biotyper database, MALDI-TOF MS could also be an applicable method.


Assuntos
Técnicas de Tipagem Bacteriana/métodos , Methylobacteriaceae/genética , Methylobacteriaceae/isolamento & purificação , RNA Ribossômico 16S/genética , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz/métodos , Sequenciamento Completo do Genoma , Adulto , Idoso , Pré-Escolar , Feminino , Humanos , Lactente , Masculino , Methylobacteriaceae/classificação , Pessoa de Meia-Idade , Filogenia , Análise de Sequência de DNA , Adulto Jovem
5.
Anaerobe ; 59: 14-18, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31075311

RESUMO

We report a case of a 58-year-old male patient who underwent several surgeries following an accident. The bacterium Robinsoniella peoriensis was detected independently in multiple samples from both the right talus and tibia. The bacterium could only be identified using 16S rRNA gene sequencing.


Assuntos
Osso e Ossos/microbiologia , Clostridiales/isolamento & purificação , Osteomielite/diagnóstico , Osteomielite/microbiologia , Ferimentos e Lesões/complicações , Clostridiales/classificação , Clostridiales/genética , Análise por Conglomerados , DNA Bacteriano/química , DNA Bacteriano/genética , DNA Ribossômico/química , DNA Ribossômico/genética , Humanos , Masculino , Pessoa de Meia-Idade , Osteomielite/patologia , Filogenia , RNA Ribossômico 16S/genética , Análise de Sequência de DNA
6.
Molecules ; 24(10)2019 May 21.
Artigo em Inglês | MEDLINE | ID: mdl-31117271

RESUMO

Raman spectra of aqueous Ho3+, Er3+, Tm3+, Yb3+, and Lu3+-perchlorate solutions were measured over a large wavenumber range from 50-4180 cm-1. In the low wavenumber range (terahertz region), strongly polarized Raman bands were detected at 387 cm-1, 389 cm-1, 391 cm-1, 394 cm-1, and 396 cm-1, respectively, which are fairly broad (full widths at half height at ~52 cm-1). These isotropic Raman bands were assigned to the breathing modes, ν1 Ln-O of the heavy rare earth (HRE) octaaqua ions, [Ln(H2O)8]3+. The strong polarization of these bands (depolarization degree ~0) reveals their totally symmetric character. The vibrational isotope effect was measured in Yb(ClO4)3 solutions in H2O and D2O and the shift of the ν1 mode in changing from H2O to D2O further supports the character of the band. The Ln-O bond distances of these HRE ions (Ho3+, Er3+, Tm3+, Yb3+, and Lu3+) follow the order of Ho-O > Er-O > Tm-O > Yb-O > Lu-O which correlates inversely with the band positions of the breathing modes of their corresponding octaaqua ions [Ln(OH2)8]3+. Furthermore, the force constants, kLn-O, were calculated for these symmetric stretching modes. Ytterbium perchlorate solutions were measured over a broad concentration range, from 0.240 mol·L-1 to 2.423 mol·L-1, and it was shown that with increasing solute concentration outer-sphere ion pairs and contact ion pairs were formed. At the dilute solution state (~0.3 mol·L-1), the fully hydrated ions [Yb(H2O)8]3+ exist, while at higher concentrations (CT > 2 mol·L-1), ion pairs are formed. The concentration behavior of Yb(ClO4)3 (aq) shows similar behavior to the one observed for La(ClO4)3(aq), Ce(ClO4)3(aq) and Lu(ClO4)3(aq) solutions. In ytterbium chloride solutions in water and heavy water, representative for the behavior of the other HRE ions, 1:1 chloro-complex formation was detected over the concentration range from 0.422-3.224 mol·L-1. The 1:1 chloro-complex in YbCl3(aq) is very weak, diminishing rapidly with dilution and vanishing at a concentration < 0.4 mol·L-1.


Assuntos
Érbio/química , Hólmio/química , Lutécio/química , Túlio/química , Itérbio/química , Íons/química , Metais Terras Raras/química , Percloratos/química , Soluções/química , Análise Espectral Raman , Água/química
7.
Molecules ; 23(12)2018 Dec 07.
Artigo em Inglês | MEDLINE | ID: mdl-30544572

RESUMO

Aqueous solutions of Lu3+- perchlorate, triflate and chloride were measured by Raman spectroscopy. A weak, isotropic mode at 396 cm-1 (full width at half height (fwhh) at 50 cm-1) was observed in perchlorate and triflate solutions. This mode was assigned to the totally symmetric stretching mode of [Lu(OH2)8]3+, ν1LuO8. In Lu(ClO4)3 solutions in heavy water, the ν1LuO8 symmetric stretch of [Lu(OD2)8]3+ appears at 376.5 cm-1. The shift confirms the theoretical isotopic effect of this mode. In the anisotropic scattering of aqueous Lu(ClO4)3, five bands of very low intensity were observed at 113 cm-1, 161.6 cm-1, 231 cm-1, 261.3 cm-1 and 344 cm-1. In LuCl3 (aq) solutions measured over a concentration range from 0.105⁻3.199 mol·L-1 a 1:1 chloro-complex was detected. Its equilibrium concentration, however, disappeared rapidly with dilution and vanished at a concentration < 0.5 mol·L-1. Quantitative Raman spectroscopy allowed the detection of the fractions of [Lu(OH2)8]3+, the fully hydrated species and the mono-chloro complex, [Lu(OH2)7Cl]2+. In a ternary LuCl3/HCl solution, a mixtrure of chloro-complex species of the type [Lu(OH2)8-nCln]+3-n (n = 1 and 2) were detected. DFT geometry optimization and frequency calculations are reported for Lu3+- water cluster in vacuo and with a polarizable dielectric continuum (PC) model including the bulk solvent implicitly. The bond distance and angle for [Lu(OH2)8]3+ within the PC are in good agreement with data from structural experiments. The DFT frequencies for the Lu-O modes of [Lu(OH2)8]3+ and its deuterated analog [Lu(OD2)8]3+ in a PC are in fair agreement with the experimental ones. The calculated hydration enthalpy of Lu3+ (aq) is slightly lower than the experimental value.


Assuntos
Lutécio/química , Água/química , Anisotropia , Teoria da Densidade Funcional , Deutério , Íons , Soluções , Análise Espectral Raman
8.
Front Immunol ; 9: 1459, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-29988557

RESUMO

Diarrhea-positive hemolytic-uremic syndrome (HUS) is a renal disorder that results from infections with Shiga-toxin (Stx)-producing Escherichia coli. The aim of this study was to establish well-defined refined murine models of HUS that can serve as preclinical tools to elucidate molecular mechanisms of disease development. C57BL/6J mice were subjected to different doses of Stx2 purified from an E. coli O157:H7 patient isolate. Animals received 300 ng/kg Stx2 and were sacrificed on day 3 to establish an acute model with fast disease progression. Alternatively, mice received 25 ng/kg Stx2 on days 0, 3, and 6, and were sacrificed on day 7 to establish a subacute model with moderate disease progression. Indicated by a rise in hematocrit, we observed dehydration despite volume substitution in both models, which was less pronounced in mice that underwent the 7-day regime. Compared with sham-treated animals, mice subjected to Stx2 developed profound weight loss, kidney dysfunction (elevation of plasma urea, creatinine, and neutrophil gelatinase-associated lipocalin), kidney injury (tubular injury and loss of endothelial cells), thrombotic microangiopathy (arteriolar microthrombi), and hemolysis (elevation of plasma bilirubin, lactate dehydrogenase, and free hemoglobin). The degree of complement activation (C3c deposition), immune cell invasion (macrophages and T lymphocytes), apoptosis, and proliferation were significantly increased in kidneys of mice subjected to the 7-day but not in kidneys of mice subjected to the 3-day regime. However, glomerular and kidney volume remained mainly unchanged, as assessed by 3D analysis of whole mount kidneys using CD31 staining with light sheet fluorescence microscopy. Gene expression analysis of kidneys revealed a total of only 91 overlapping genes altered in both Stx2 models. In conclusion, we have developed two refined mouse models with different disease progression, both leading to hemolysis, thrombotic microangiopathy, and acute kidney dysfunction and damage as key clinical features of human HUS. While intrarenal changes (apoptosis, proliferation, complement deposition, and immune cell invasion) mainly contribute to the pathophysiology of the subacute model, prerenal pathomechanisms (hypovolemia) play a predominant role in the acute model. Both models allow the further study of the pathomechanisms of most aspects of human HUS and the testing of distinct novel treatment strategies.

9.
Emerg Microbes Infect ; 7(1): 61, 2018 Apr 04.
Artigo em Inglês | MEDLINE | ID: mdl-29618738

RESUMO

The genus Myroides comprises several species of Gram-negative, non-motile, and non-fermenting bacteria, which have been regarded as non-pathogenic for decades. Multiple recent reports, however, underscore the pathogenic potential that Myroides sp. possesses for humans. These bacteria seem to be resistant to a wide range of antibiotics (including ß-lactams and aminoglycosides). Therefore, treatment options are limited. Knowledge of antimicrobial resistance, however, is based on only one meaningful comprehensive study and on data published from case reports. This lack of data motivated us to test 59 strains from our Myroides collection (43 M. odoratimimus and 16 M. odoratus) for resistance against 20 commonly used antibiotics. We also performed molecular analyses to reveal whether our bacteria harbor the genus-specific M. odoratimimus metallo-ß-lactamase (MUS-1) or the M. odoratus metallo ß-lactamase (TUS-1), and other ß-lactamases, which may provide an explanation for the extended antimicrobial resistance.


Assuntos
Antibacterianos/farmacologia , Flavobacteriaceae/efeitos dos fármacos , Flavobacteriaceae/genética , Sequenciamento Completo do Genoma , DNA Bacteriano , Flavobacteriaceae/classificação , Flavobacteriaceae/enzimologia , Humanos , Testes de Sensibilidade Microbiana , Filogenia , RNA Ribossômico 16S , Análise de Sequência de DNA , beta-Lactamases/biossíntese , beta-Lactamases/genética
10.
Dalton Trans ; 46(13): 4235-4244, 2017 Mar 27.
Artigo em Inglês | MEDLINE | ID: mdl-28280811

RESUMO

Raman spectra of aqueous La3+, Ce3+, Pr3+, Nd3+ and Sm3+ - perchlorate solutions were measured and weak strongly polarized Raman bands were detected at 343 cm-1, 344 cm-1, 347 cm-1, 352 cm-1 and 363 cm-1, respectively. The full width at half height for these bands is quite broad (∼50 cm-1) in the isotropic spectrum and the band width increases with increasing solute concentration. The polarized Raman bands were assigned to the breathing modes of the nona-aqua ions of the mentioned rare earth ions. Published structural results confirmed that these ions exist as nona-hydrates in aqueous solutions [Ln(H2O)9]3+. The Ln-O bond distances of these rare earth ions correlate well with the band positions of the nona-aqua ions [Ln(OH2)9]+3 (Ln = La3+, Ce3+, Pr3+, Nd3+ and Sm3+) and the force constants were calculated for these breathing modes. The strength of the force constants increase with decreasing the Ln-O bond distances (La-O > Ce-O > Pr-O > Nd-O > Sm-O). While the fully hydrated ions are stable in dilute perchlorate solutions (∼0.2 mol L-1), in concentrated perchlorate solutions outer-sphere ion pairs and contact ion pairs are formed (C > 1.5 mol L-1). In a hydrate melt at 161 °C of Ce(ClO4)3 plus 6H2O, the contact ion pairs are the dominate species. The Raman bands of the ligated perchlorate and the Ce-O breathing mode of the partially hydrated ion pair at 326 cm-1 were measured and characterized. In cerium chloride solutions chloro-complex formation was detected over the measured concentration range from 0.270-2.167 mol L-1. The chloro-complexes in CeCl3(aq) are weak and diminish rapidly with dilution and disappear at a concentration <0.1 mol L-1. In a CeCl3 solution, with additional HCl, a series of chloro-complex species of the type [Ce(OH2)9-nCln]+3-n (n = 1, 2) were detected.

12.
J Vis Exp ; (113)2016 07 11.
Artigo em Inglês | MEDLINE | ID: mdl-27500532

RESUMO

There are a number of rare and, therefore, insufficiently described bacterial pathogens which are reported to cause severe infections especially in immunocompromised patients. In most cases only few data, mostly published as case reports, are available which investigate the role of such pathogens as an infectious agent. Therefore, in order to clarify the pathogenic character of such microorganisms, it is necessary to conduct epidemiologic studies which include large numbers of these bacteria. The methods used in such a surveillance study have to meet the following criteria: the identification of the strains has to be accurate according to the valid nomenclature, they should be easy to handle (robustness), economical in routine diagnostics and they have to generate comparable results among different laboratories. Generally, there are three strategies for identifying bacterial strains in a routine setting: 1) phenotypic identification characterizing the biochemical and metabolic properties of the bacteria, 2) molecular techniques such as 16S rRNA gene sequencing and 3) mass spectrometry as a novel proteome based approach. Since mass spectrometry and molecular approaches are the most promising tools for identifying a large variety of bacterial species, these two methods are described. Advances, limitations and potential problems when using these techniques are discussed.


Assuntos
Bactérias/classificação , Infecções Bacterianas/diagnóstico , Proteômica/métodos , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz , Humanos , RNA Ribossômico 16S/genética
13.
Appl Microbiol Biotechnol ; 100(24): 10479-10493, 2016 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-27430741

RESUMO

Wild-type human interleukin-10 (hIL-10) is a non-covalent homodimer with a short half-life, thus limiting its therapeutic applications in vivo. To avoid loss of function due to dimer dissociation, we designed a synthetic hIL-10 analog by bridging both monomers via a 15 amino acid-long peptide spacer in a C-terminal to N-terminal fashion. For secretory expression in Escherichia coli, a 1156 bp fragment was generated from template vector pAZ1 by fusion PCR encoding a T7 promoter region and the signal sequence of the E. coli outer membrane protein F fused in frame to two tandem E. coli codon-optimized mature hIL-10 genes connected via a 45 nucleotide linker sequence. The construct was cloned into pUC19 for high-level expression in E. coli BL21 (DE3). The mean concentrations of hIL-10 fusion protein in the periplasm and supernatant of E. coli at 37 °C growth temperature were 130 ± 40 and 2 ± 1 ng/ml, respectively. The molecular mass of the recombinant protein was assessed via matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) analysis, indicating correct processing of the signaling sequence in E. coli. In vitro biological activity was shown by phosphorylation of signal transducer and activator of transcription protein 3 and suppression of tumor necrosis factor α secretion in lipopolysaccharide-stimulated macrophages.


Assuntos
Escherichia coli/metabolismo , Expressão Gênica , Interleucina-10/metabolismo , Proteínas Recombinantes de Fusão/metabolismo , Clonagem Molecular , Escherichia coli/genética , Escherichia coli/crescimento & desenvolvimento , Interleucina-10/química , Interleucina-10/genética , Peso Molecular , Proteínas Recombinantes de Fusão/química , Proteínas Recombinantes de Fusão/genética , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz
14.
Dalton Trans ; 44(42): 18492-505, 2015 Nov 14.
Artigo em Inglês | MEDLINE | ID: mdl-26442624

RESUMO

Raman spectra of aqueous yttrium perchlorate, triflate (trifluoromethanesulfonate), chloride and nitrate solutions were measured over a broad concentration range (0.198-3.252 mol L(-1)). The spectra range from low wavenumbers to 4200 cm(-1). A very weak mode at 384 cm(-1) with a full width at half height at 50 cm(-1) in the isotropic spectrum suggests that the Y(3+)- octa-aqua ion is thermodynamically stable in dilute perchlorate solutions (∼0.5 mol L(-1)) while in concentrated perchlorate solutions outer-sphere ion pairs and contact ion pairs are formed. The octa-hydrate, [Y(OH2)8](3+) was also detected in a 1.10 mol L(-1) aqueous Y(CF3SO3)3 solution. Furthermore, very weak and broad depolarized modes could be detected which are assigned to [Y(OH2)8](3+)(aq) at 100, 166, 234 and 320 cm(-1) confirming that a hexa-hydrate is not compatible with the hydrated species in solution. In yttrium chloride solutions contact ion pair formation was detected over the measured concentration range from 0.479-3.212 mol L(-1). The contact ion pairs in YCl3(aq) are fairly weak and disappear with dilution. At a concentration <0.2 mol L(-1) almost all complexes have disappeared. In YCl3 solutions, with additional HCl, chloro-complexes of the type [Y(OH2)8-nCln](+3-n) (n = 1,2) are formed. The Y(NO3)3(aq) spectra were compared with a spectrum of a dilute NaNO3 solution and it was concluded that in Y(NO3)3(aq) over the concentration range from 2.035-0.198 mol L(-1) nitrato-complexes [Y(OH2)8-n(NO3)ln](+3-n) (n = 1,2) are formed. The nitrato-complexes are weak and disappear with dilution <0.1 mol L(-1). DFT geometry optimizations and frequency calculations are reported for both the yttrium-water cluster in the gas phase and the cluster within a polarizable continuum model in order to implicitly describe the presence of the bulk solvent. The bond distance and angle for the square antiprismatic cluster geometry of [Y(OH2)8](3+) with the polarizable dielectric continuum is in good agreement with data from recent structural experimental measurements. The DFT frequency of the Y-O stretching mode of the [Y(OH2)8](3+) cluster, in a polarizable continuum, is at 372 cm(-1) in satisfactory agreement with the experimental value.

15.
Dalton Trans ; 44(1): 295-305, 2015 Jan 07.
Artigo em Inglês | MEDLINE | ID: mdl-25379866

RESUMO

Raman spectra of aqueous lanthanum perchlorate, triflate (trifluorosulfonate), chloride and nitrate solutions were measured over a broad concentration (0.121-3.050 mol L(-1)) range at room temperature (23 °C). A very weak mode at 343 cm(-1) with a full width at half height at 49 cm(-1) in the isotropic spectrum suggests that the nona-aqua La(III) ion is thermodynamically stable in dilute perchlorate solutions (∼0.2 mol L(-1)) while in concentrated perchlorate solutions outer-sphere ion pairs and contact ion pairs are formed. The La(3+) nona-hydrate was also detected in a 1.2 mol L(-1) La(CF3SO3)3(aq). In lanthanum chloride solutions chloro-complex formation was detected over the measured concentration range from 0.5-3.050 mol L(-1). The chloro-complexes in LaCl3(aq) are fairly weak and disappear with dilution. At a concentration <0.1 mol L(-1) almost all complexes disappeared. In LaCl3 solutions, with additional HCl, a series of chloro-complexes of the type [La(OH2)(9-n)Cln](+3-n) (n = 1-3) were formed. The La(NO3)3(aq) spectra were compared with a spectrum of a 0.409 mol L(-1) NaNO3(aq) and it was concluded that in La(NO3)3(aq) over the concentration range from 0.121-1.844 mol L(-1), nitrato-complexes, [La(OH2)(9-n)(NO3)n](+3-n) (n = 1, 2) were formed. These nitrato-complexes are quite weak and disappear with dilution <0.01 mol L(-1). DFT geometry optimizations and frequency calculations are reported for a lanthanum-nona-hydrate with a polarizable dielectric continuum in order to take the solvent into account. The bond distances and angles for the cluster geometry of [La(OH2)9](3+) with the polarizable dielectric continuum are in good agreement with data from recent structural experimental measurements and high quality simulations. The DFT frequency of the La-O stretching mode at 328.2 cm(-1), is only slightly smaller than the experimental one.

16.
Int J Infect Dis ; 29: 71-3, 2014 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-25461238

RESUMO

The isolation of Aureimonas altamirensis (a rare opportunistic pathogen with a yet unresolved pathogenicity) from the ascites fluid of a patient with bacterial peritonitis is reported. The strain was first identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and the result was confirmed using 16S rDNA sequencing. An antimicrobial susceptibility profile was determined according to the European Committee on Antimicrobial Susceptibility Testing (EUCAST) guidelines published in 2013, revealing sensitivity to all antibiotics tested. The patient was treated effectively with levofloxacin.


Assuntos
Alphaproteobacteria/isolamento & purificação , Infecções Bacterianas/microbiologia , Peritonite/microbiologia , Alphaproteobacteria/efeitos dos fármacos , Alphaproteobacteria/genética , Infecções Bacterianas/diagnóstico , Farmacorresistência Bacteriana , Feminino , Humanos , Pessoa de Meia-Idade , Peritonite/diagnóstico
17.
Diagn Microbiol Infect Dis ; 79(2): 155-9, 2014 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-24666701

RESUMO

The genus Myroides comprises the 2 medically relevant species Myroides odoratus and Myroides odoratimimus that are rare opportunistic pathogens and cause infections in immunocompromised patients. A fast identification of Myroides is of importance because these bacterial strains show multiple resistance against antibiotics and therefore limit treatment options. They are associated, for instance, with urinary tract infections, sepsis, meningitis, pneumonia, and infectious cellulitis. Since more and more Myroides spp. are being described, additional potentially pathogenic bacteria may be identified in the future demanding the need for fast and reliable identification methods at species level. However, to date, only molecular approaches meet these demands. In this study, we, therefore, attempt to define an appropriate method other than DNA fingerprinting that will permit a comparable efficacy and, possibly, a more economical strain identification. For this purpose, we compared 2 widely used automated diagnostic systems (VITEK 2 [bioMérieux, Nürtingen, Germany] and Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS) [Bruker Daltonics, Bremen, Germany]) and correlated the results to 16S rDNA sequencing data. In total, we analyzed 22 strains collected in the course of routine diagnostics. In this study, we demonstrate that VITEK 2 reliably identifies the genus Myroides but cannot differentiate between M. odoratimimus and M. odoratus. In contrast to this, both MALDI-TOF MS and 16S rDNA sequencing efficiently distinguish between the 2 species.


Assuntos
Técnicas de Tipagem Bacteriana/métodos , Infecções por Flavobacteriaceae/diagnóstico , Flavobacteriaceae/classificação , Flavobacteriaceae/isolamento & purificação , Técnicas de Diagnóstico Molecular/métodos , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz/métodos , DNA Ribossômico/química , DNA Ribossômico/genética , Infecções por Flavobacteriaceae/microbiologia , Humanos , RNA Ribossômico 16S/genética , Análise de Sequência de DNA/métodos
18.
Dalton Trans ; 43(8): 3174-85, 2014 Feb 28.
Artigo em Inglês | MEDLINE | ID: mdl-24346353

RESUMO

Aqueous solutions of sodium acetate, NaCH3CO2, and acetic acid, CH3COOH, were studied using Raman and infrared spectroscopy. The spectra were recorded over a large concentration range, in the terahertz region and up to 4000 cm(-1). In the isotropic Raman spectrum in R-format, a polarized band at 189 cm(-1) was assigned to the ν1Na-O stretch of the hydrated Na(+)-ion and a shoulder at 245 cm(-1) to the restricted translation band, νsO-H···O* of the hydrated acetate ion, CH3CO2(-)(aq). The CH3CO2(-)(aq) and the hydrated acetic acid, CH3COOH(aq), possess pseudo Cs symmetry. Geometrical parameters for the species in the gas phase and for CH3CO2(-)(aq) and CH3COOH(aq) are reported. Characteristic bands for CH3CO2(-)(aq) and CH3COOH(aq) were assigned under the guidance of the DFT vibrational frequency calculations and discussed in detail. In aqueous NaCH3CO2 solutions, at high concentrations, no contact ion pairs could be detected, but instead solvent separated ion pairs were found. In LiCH3CO2(aq), however, contact ion pairs are formed which is indicated by the appearance of a shoulder at 939 cm(-1) and the shift of the symmetric stretching mode of the -CO2(-) group to higher wavenumbers.


Assuntos
Ácido Acético/química , Acetato de Sódio/química , Modelos Moleculares , Soluções/química , Espectrofotometria Infravermelho , Análise Espectral Raman , Vibração
19.
Ger Med Sci ; 11: Doc14, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-24068980

RESUMO

We report on the isolation of Actinobacillus equuli ssp. haemolyticus from wound smears of a 2-year-old girl who was admitted to the hospital due to partial amputation of the distal phalanx of her right middle finger caused by a horse bite. A. equuli typically causes diseases in horses and only very few reports describing human infections (mostly associated with wounds) are available in the literature. Interestingly, although the bacteria could be found in consecutive samples taken at different points in time, there were no signs of advancing infection or inflammation. Moreover, the fingertip regenerated after 74 days under semi-occlusive dressings with very pleasant results. For strain identification two automated systems were employed producing discrepant results: VITEK 2 described the pathogens as Pasteurella pneumotropica while MALDI-TOF MS analysis revealed A. equuli. Sequence analysis of 16S rDNA gene finally confirmed A. equuli ssp. haemolyticus as the isolated strain. The antimicrobial susceptibility testing was performed according to the CLSI criteria for Pasteurella spp. Additionally we conducted a test according to the EUCAST criteria.


Assuntos
Infecções por Actinobacillus/terapia , Actinobacillus equuli/isolamento & purificação , Antibacterianos/uso terapêutico , Bandagens , Mordeduras e Picadas/terapia , Cavalos/microbiologia , Infecções por Actinobacillus/diagnóstico , Infecções por Actinobacillus/microbiologia , Animais , Mordeduras e Picadas/diagnóstico , Mordeduras e Picadas/microbiologia , Pré-Escolar , Feminino , Humanos , Resultado do Tratamento
20.
Dalton Trans ; 42(40): 14460-72, 2013 Oct 28.
Artigo em Inglês | MEDLINE | ID: mdl-23969599

RESUMO

Raman spectroscopic measurements have been performed on aqueous solutions of Mn(ClO4)2, MnCl2, MnBr2, and Mn(NO3)2 in the terahertz region (40-600 cm(-1)) and to wavenumbers up to 4200 cm(-1) employing an absorption gap in these light pink coloured solutions. In Mn(ClO4)2 solutions of water and heavy water, the hexahydrate and its deuterate, [Mn(OH2)6](2+) and [Mn(OD2)6](2+), were characterized as showing a very weak, strongly polarized band at 354 cm(-1) and 338 cm(-1), respectively. These modes were assigned to ν1 MnO6 of [Mn(OH2)6](2+) and [Mn(OD2)6](2+). In Mn(NO3)2(aq), the undisturbed mode at 354 cm(-1), representative of manganese hexahydrate, was also detected in dilute solutions up to ~3 mol L(-1) and no sign of nitrato complex formation could be obtained. In fairly dilute MnCl2(aq) up to ~1.5 mol L(-1) the hexaaqua ions were identified but in concentrated solutions, chloro-complexes of Mn(2+) were detected in the form of [Mn(OH2)(6-n)Cl(n)]((+2-n)) with n equal to one or two. In MnBr2(aq) a comparable picture to the one in MnCl2(aq) could be obtained. Density functional theory (DFT) calculations on Mn(2+) water clusters were carried out to optimize the geometry and calculate the frequencies of the [Mn(OH2)6](2+) cluster. For this purpose an unrestricted B3LYP functional was used with a triple-ζ basis set 6-311+G(d,p). In order to include the hydration effects around the [Mn(OH2)6](2+), a continuum model approach was employed where the solvent is described by a structureless dielectric polarizable continuum using the Polarizable Continuum Model of Solvation (PCM). The gas phase cluster of [Mn(OH2)6](2+) led to lower MnO6 frequencies compared to the measured ones. A second, much larger cluster model, with 18 water molecules containing 6 waters in the first shell and an explicit second hydration shell, [Mn(OH2)6(OH2)12](2+), was modelled. Again, the cluster in vacuo and the cluster surrounded by a structureless polarizable continuum were considered. The larger cluster including the polarizable continuum gives the most realistic frequency value of ν1 MnO6 and the other MnO6 skeleton modes. The hydration enthalpy, ΔH(hyd(l)) at 298 K, of [Mn(OH2)6](2+)(aq) was calculated by applying a Born-Haber cycle and correcting for the heat of vaporization, ΔH(vap), of water and the solvation enthalpy, ΔH(solv), released by transferring the gas phase cluster into the solution. The theoretical hydration enthalpy is in fair agreement with the measured hydration enthalpy.


Assuntos
Brometos/química , Cloretos/química , Compostos de Manganês/química , Nitratos/química , Percloratos/química , Ânions/química , Teoria Quântica , Soluções , Análise Espectral Raman , Água/química
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