Evaluation of antimicrobial efficacy of immunoglobulin Y (IgY) against periodontal biofilm.

Background and Objectives: To determine the action of immunoglobulin Y (IgY) on supragingival microbiota and on subgingival microbiota in patients with gingivitis and periodontitis through microbial reduction assay. Methodology: 40 systemically healthy patients were divided into two groups (gingivitis and periodontitis) with 20 patients per group. Supragingival and subgingival plaque samples were collected from each patient in Group I and Group II, respectively. Sample 1 and Sample 2 from each patient were immediately transferred into sterile Eppendorf tube 1 and tube 2 with microbial transport media, respectively. Both the tubes were then immediately transferred into an anaerobic jar and sent to the microbial facility. IgY was then added to these samples. All the samples were collected in duplicate vials to check the in vitro antimicrobial activity of microbes with IgY and without IgY. Microbial reduction percentage was calculated based on the colony count comparing the colonies with and without IgY. Results: The mean CFUs in the gingivitis group with IgY samples was significantly lesser as compared to the periodontitis group. The mean CFUs in gingivitis and periodontitis group with IgY samples was significantly lesser as compared to those without IgY samples. Conclusion: IgY has a significant role in the reduction of bacterial count in supragingival and subgingival plaque samples. So, IgY when used as a local drug delivery agent or mouthwash, as an adjunct to scaling and root planing may reduce gingival and periodontal diseases but further studies showing its effect must be carried out to validate the same.

Analysis of antimicrobial activity of Karnataka propolis against oral pathogens - An in vitro study.

Background: Propolis, a natural antibiotic, which is in high demand in dentistry is a resinous substance. The main ingredient of propolis that is required for antibiotic effect is flavonoids and phenolic acids. Although propolis is a promising option for the control of oral microbes with lower related hazards and a good immunomodulator effect, its composition differs considerably depending on its botanical origin, the site and the season of collection. This original research aims to find the chemical composition and minimum inhibitory concentration (MIC) of propolis procured from different places of Karnataka state. The results would help the dentist and the pharmacist to select the best propolis to use as antibiotics in treating oral disease. Materials and Methods: Propolis sample from 5 different locations of Karnataka was procured from single apiary in Bangalore. Extraction of propolis using two different extracting solvents was carried out. The total phenolic content, total flavonoid content and MIC of each sample were analyzed. Results: Water extract propolis of Sullia and Hubli was highly active against tested organism with the MIC <0.312; alcohol extract of Sullia, Hubli and Chitradurga was moderately active with the MIC between 0.312 and 5 mg/ml. Vijayapura and Bagalkot were least active with the MIC >5 mg/ml at tested concentration. Conclusion: Propolis procured from different locations of Karnataka can be used as an antimicrobial agent with varying concentrations. However, when propolis is procured for therapeutic purpose, then it needs to be tested for its chemical composition before being utilized.

Malocclusion and related quality of life among 13- to 19-year-old students in Mysore City - a cross-sectional study.

PURPOSE: To assess malocclusion and related quality of life among 13- to 19-year-old students in Mysore City. MATERIALS AND METHODS: A total of 900 students aged 13-19 years selected based on multistage stratified random sampling were included in this cross-sectional study. A pre-designed format was used to record the sociodemographic details, oral hygiene practices, personal habits, malocclusion and oral health related quality of life. Malocclusion was assessed using Dental Aesthetic Index (DAI ) and quality of life using Oral Health Impact Profile-14 (OHIP-14). Further, the association between malocclusion and related quality of life was assessed. RESULTS: Normal or slight malocclusion was seen in 79.2% (n = 713). Definite malocclusion was seen in 12.8%, severe malocclusion in 5.7% and handicapping malocclusion in 2.3% of the subjects. The overall prevalence of malocclusion was found to be 20.8%. Out of the 900 study subjects 46.6% (n = 419) reported that malocclusion had some impact on their oral health related quality of life. The results of logistic regression demonstrated that those with malocclusion experienced a significant impact on quality of life (OR = 16.07, CI = 9.17-28.17). CONCLUSION: Malocclusion has an impact on the oral health related quality of life among 13- to 19-year-old students in Mysore City.

Antiplaque and antigingivitis efficacy of triphala and chlorhexidine mouthrinse among schoolchildren - a cross-over, double-blind, randomised controlled trial.

PURPOSE: To evaluate and compare the effect of triphala extract mouthrinse and chlorhexidine on dental plaque and gingivitis. MATERIALS AND METHODS: In this double blind, crossover study, 120 qualifying boarding-school students aged 13-16 years were randomised into three groups: 10% triphala, 0.2% chlorhexidine and negative control. The study was conducted in 3 phases of 1-month duration each and a washout period of 15 days. During the experimental period, subjects rinsed with the allocated mouthrinse once daily for 30 s under supervision. The plaque and gingival status was assessed using the Turesky modification of the Quigley and Hein plaque index (QHI) and the gingival index (Löe and Silness) at baseline and at the end of each phase. The results were tested for significance at P < 0.05. RESULTS: Triphala and chlorhexidine yielded a significant reduction in plaque and gingival index scores as compared to negative control (P < 0.001). No significant difference was found between the scores obtained with triphala and chlorhexidine mouthwashes. CONCLUSION: The antiplaque and antigingivitis activity of triphala closely parallels that of chlorhexidine.

Assessment of Microleakage in Class II Cavities having Gingival Wall in Cementum using Three Different Posterior Composites.

BACKGROUND: Dental composites are one of the most desired restorative materials today. Composite materials can be bonded successfully to human tooth enamel; however, developing the same degree of adhesion to dentin or cementum is a more challenging task. Polymerization contraction stress of dental composites is often associated with marginal and interfacial failures of bonded restorations. The magnitude of stress depends on composite composition and its ability to flow before gelation, which is related to the cavity configuration and curing characteristics of the composite. MATERIALS AND METHODS: This study was carried out on 24 extracted human molars and divided into three groups. Class II (slot) cavities were prepared on the mesial and distal surfaces of the teeth with the gingival wall in dentin/cementum, and the microleakage was observed. After preparation the teeth were randomly assigned into three groups of eight specimens each. The cavities were restored with: Group 1: Packable composite (Surefil, Dentsply); Group 2: Hybrid composite (Filtek Z250, 3M Dental Products); Group 3: Nanocomposite (Filtek Z350, 3M Dental Products). Sixteen samples of each group were subjected to 500 cycles of thermocycling between 5°C and 55°C. All the teeth were immersed in methylene blue for 8 h and then left in tap water for 12 h. The teeth were sectioned mesiodistally with a diamond disc, and examined under a stereomicroscope. RESULTS: The analysis indicated that packable composite showed more microleakage than all the other groups. Hybrid composite showed less microleakage than packable composite, but more leakage than nanocomposite. CONCLUSION: All composites under the study exhibited a certain amount of microleakage in the dentin/cementum margin. Packable composite showed the most microleakage and nanocomposite showed least microleakage. Since these results were obtained in vitro, long-term clinical trials are needed to fully understand the performance of these materials.

Oral health-related quality of life and nutritional status of institutionalized elderly population aged 60 years and above in Mysore City, India.

OBJECTIVE: To assess whether oral health-related quality of life (OHRQoL) is associated with nutritional status in the institutionalised elderly population of Mysore. BACKGROUND: Malnutrition in the elderly has an evident impact on their general health and quality of life. Analysis of data of the Geriatric Oral Health Assessment Index (GOHAI) and their association with the Mini Nutritional Assessment (MNA) results improves our understanding of the complex relationship between oral health and malnutrition. MATERIALS AND METHODS: The study was conducted among the institutionalised elderly population in Mysore city, Karnataka. Data on socio-demographic, oral health status were gathered. OHRQoL was evaluated using GOHAI, and malnutrition risk using MNA. RESULTS: Out of 141 elderly, 41.1% were men and 58.9% were women with mean age of 72.2 ±7.5 years. Mean GOHAI score was 47.03 ± 9.2, with 69.5% had low perception of oral health. Mean MNA score was 9.91 ± 2.4, 15.6% were malnourished, 52.5% were at risk of malnutrition and 31.9% were adequately nourished. A strong association was found between the mean GOHAI and MNA scores. CONCLUSION: Oral health-related quality of life was associated with nutritional deficit, and it requires a greater integration between dentistry and nutrition in the health promotion of older adults.

Staff's attitude regarding hospital waste management in the dental college hospitals of Bangalore city, India.

BACKGROUND: Growing urbanization has led to several changes in the healthcare sector. While on one hand, access to healthcare services are being provided to the community, thereby resulting in better health for all, improper management of biomedical waste emanating from these healthcare establishments has also given rise to many environmental and health problems (Acharya 2003). Although awareness in this issue has increased considerably over the last few years, sensitivity to this problem has been limited. Most hospitals are not actively involved in addressing this problem. MATERIALS AND METHODS: The present study was conducted to assess the attitude of waste management among staff of dental hospitals in Bangalore city, by issuing a pre-tested structured questionnaire. RESULTS: While 82.6% of attenders said that it is necessary to segregate waste into different categories at the point of origin, 61.5% of auxiliaries strongly disagreed that segregation of waste at source increases the risk of injury to waste handlers. As many as 33.5% of dentists strongly disagreed that segregation of waste at source increases the risk of injury to waste handlers and 53.6% agreed that segregation of waste at source does not increase the risk of injury to waste handlers. CONCLUSION: The findings of this study suggest that the staff had good attitude towards waste management among.