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3.
J Biotechnol ; 77(2-3): 191-208, 2000 Feb 17.
Artigo em Inglês | MEDLINE | ID: mdl-10682279

RESUMO

Batch and continuous cultures of a newly isolated Clostridium butyricum strain were carried out on industrial glycerol, the major by-product of the bio-diesel production process. For both types of cultures, the conversion yield obtained was around 0.55 g of 1,3-propanediol formed per 1 g of glycerol consumed whereas the highest 1,3-propanediol concentration, achieved during the single-stage continuous cultures was 35-48 g l-1. Moreover, the strain presented a strong tolerance at the inhibitory effect of the 1,3-propanediol, even at high concentrations of this substance at the chemostat (e.g. 80 g l-1). 1,3-Propanediol was associated with cell growth whereas acetate and butyrate seemed non growth-associated products. At low and medium dilution rates (until 0.1 h-1), butyrate production was favoured, whereas at higher rates acetate production increased. The maximum 1,3-propanediol volumetric productivity obtained was 5.5 g l-1 h-1. A two-stage continuous fermentation was also carried out. The first stage presented high 1,3-propanediol volumetric productivity, whereas the second stage (with a lower dilution rate) served to further increase the final product concentration. High 1,3-propanediol concentrations were achieved (41-46 g l-1), with a maximum volumetric productivity of 3.4 g l-1 h-1. A cell concentration decrease was reported between the second and the first fermentor.


Assuntos
Clostridium/metabolismo , Glicerol/metabolismo , Propilenoglicóis/metabolismo , Reatores Biológicos , Indústria Química , Clostridium/crescimento & desenvolvimento
4.
Med Vet Entomol ; 4(3): 311-4, 1990 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-2132996

RESUMO

The prevalence of red deer hypodermosis and the life cycle of Hypodermia diana Brauer 1858 in three provinces of the south of Spain, Córdoba, Jaen and Ciudad Real, were studied by inspecting 254, thirty-eight and thirty-five deer from each province respectively. The prevalence of infestations was: Córdoba, 87.75%; Jaen, 92.10%, Ciudad Real, 91.42%. From this we deduced an overall prevalence of 88.67%, comprising 88.23% in male deer and 89.96% in female deer. The intensity of the parasitism in 213 of the 245 animals from Córdoba varied between one warble per animal to more than 100 in 28 of the inspected animals. The chronology of the life cycle during the hunting period (October to the end of February) was investigated.


Assuntos
Cervos/parasitologia , Dípteros/crescimento & desenvolvimento , Hipodermose/veterinária , Animais , Dípteros/isolamento & purificação , Feminino , Hipodermose/epidemiologia , Larva/isolamento & purificação , Masculino , Prevalência , Estações do Ano , Espanha/epidemiologia
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