RESUMO
Introducción. La detección de cepas de Escherichia coli enteroagregativas (ECEA) mediante la técnica de referencia (adherencia celular) presenta problemas de implementación metodológica en rutina hospitalaria. Métodos. Se validó la técnica de reacción en cadena de la polimerasa (PCR), en comparación con la de adherencia celular.Resultados. La PCR presentó, respecto a la técnica de adherencia a cultivo celular: sensibilidad de 0,93, especificidad de 1, valor predictivo positivo de 1 y valor predictivo negativo de 0,91. Conclusión. La PCR es alternativa viable para la detección habitual de cepas ECEA (AU)
Introduction. Detection of enteroaggregative strains of Escherichia coli (EAEC) by the gold standard technique (cellular adherence) is difficult to implement in routine clinical practice. Methods. The gold standard was compared with a PCR method for this purpose. Results. With respect to the cellular adherence technique, PCR performance was as follows: sensitivity 0.93, specificity 1, positive predictive value 1, and negative predictive value 0.91.Conclusion. The PCR method studied is a viable alternative for routine detection of EAEC strains (AU)
Assuntos
Humanos , Diarreia/microbiologia , Escherichia coli/isolamento & purificação , Infecções por Escherichia coli/microbiologia , Reação em Cadeia da Polimerase/métodos , Escherichia coli/patogenicidade , Adesão CelularRESUMO
INTRODUCTION: Detection of enteroaggregative strains of Escherichia coli (EAEC) by the gold standard technique (cellular adherence) is difficult to implement in routine clinical practice. METHODS: The gold standard was compared with a PCR method for this purpose. RESULTS: With respect to the cellular adherence technique, PCR performance was as follows: sensitivity 0.93, specificity 1, positive predictive value 1, and negative predictive value 0.91. CONCLUSION: The PCR method studied is a viable alternative for routine detection of EAEC strains.
