[Introduction to forensic gerontology]. / Vvedenie v sudebno-meditsinskuyu gerontologiyu.

Objective - to substantiate the identification of a new direction in forensic medicine - forensic gerontology. A statistical analysis of the structure of forensic medical research of corpses in terms of age for a twenty-year period has been carried out. The data on the features of the expert assessment of injuries in old age taking into account geriatric syndromes and aging syndromes have been analyzed. The features and difficulties of postmortem diagnosis among persons aged 75+ in cases of nonviolent death were assessed and a significant disproportion in the structure of morbidity with pathology detected during autopsy was noted. The data obtained made it possible to substantiate the selection of a new direction of forensic science and outline the prospects for research in this area.

A Novel Method for Amyloid Detection in Human Tissue Load Using a Fluorescent Dye - Congo Red Analogue.

The aim of the study was to develop a new technology for the detection of amyloid in human tissues based on the fluorescent dye, disodium salt of 2,7-(1-amino-4-sulfo-2-naphthylazo)fluorene (DSNAF). MATERIALS AND METHODS: Synthesis of DSNAF was performed by diazotization of 2,7-diaminofluorene in a stream of argon followed by azo coupling with naphthionic acid. Identification of DSNAF was performed using MALDI mass spectrometry. Human myocardial samples from males and females aged from 85 to 98 years (n=11) were the material for the histochemical study. Myocardial paraffin sections were stained with a 0.1% aqueous solution of Congo red or with an aqueous solution (0.1 or 0.034%) of DSNAF under the same conditions. RESULTS: It has been demonstrated for the first time that a new fluorene-based analogue of Congo red, DSNAF, can be successfully used to identify amyloid deposits in histological sections of human myocardium. In terms of the specificity and intensity of amyloid staining, DSNAF is comparable to Congo red, which is the gold standard for detecting amyloid deposits. The fluorescence intensity of DSNAF when binding to amyloid fibrils is significantly higher than the intensity of Congo red fluorescence (with a lower intensity of background fluorescence of heart muscle tissue). This is especially useful for identifying small deposits of amyloid in the human tissues which is important when using small biopsies. CONCLUSION: The advantages of using DSNAF allow us to consider the developed technology for the detection of amyloid as a new promising method of identifying amyloid deposits in human tissues.