RESUMO
Objective To study the effect of Gabapentin(GBP)on the transient receptor potential vanilloid l(TRPV1)in dorsal root ganglion (DRG)of rats with chronic constriction injury(CCI). Methods SD male rats were used to establish CCI model and randomly divided into 3 groups,the control group,the CCI group,and the CCI+GBP group. Mechanical allodynia and thermal hyperalgesia were measured to evaluate the es?tablishment of CCI model and the analgesic effect of GBP. At the postoperative 8 days,all rats were sacrificed and dorsal root ganglions were collect?ed for further RT?PCR and Western?blotting analysis to detect expression of TRVP1 in DRG. Results The mRNA and protein expression of TRPV1 were both significantly increased in DRG after CCI operation and GBP could decrease mRNA expression of TRPV1 gene in DRG after CCI. Conclusion The expression of TRPV1 was increased in DRG after CCI operation which was decreased after administration of GBP.
RESUMO
Objective To investigate the feature of pathogenic bacteria in central venous catheter related infection (CVC-RI)and risk factor in the emergency intensive care unit (ICU).Methods One hundred and three patients were checked out bacteria from January 2006 to December 2008 in the ICU who were central venous catheter and corresponded with diagnostic criteria for CVC-RI.They were analyzed retrospectively.Results The ratio between Gram-postive bacteria (37.9%,39/103)and Gram-negative bacteria (42.7%,44/103)were not statistically significant,however,CVC-RI was related to applying duetal number,duct keeping on time and elect location.Duct staying in subclavian vein had the fewest chance.Conclusions It would be better to choose duct staying in subclavian vein.control remaining time and luminal number,and monitor changing pathogenic bacteria strictly.Main measures are taken to evade the pollution on the ental and external lumina.
